Calcium phosphate ceramic coatings on porous titanium: effect of structure and composition on electrophoretic deposition, vacuum sintering and in vitro dissolution

One of the most common causes of implant failure is aseptic prosthesis loosening. Another frequent complication after prosthesis implant is the microbial colonization of the prosthesis surface, which often leads to a replacement of the prosthesis. One approach to reduce these complications is the application of bioactive substances to implant surfaces. Both an antibiotic prophylaxis and a faster osteointegration can be obtained by incorporation of bactericidal active metals in degradable calcium phosphate (CaP) coatings. In this study, thin degradable calcium phosphate ceramic coatings doped with silver (Ag), copper (Cu), and bismuth (Bi) on a titanium substrate were prepared with the aid of the high-velocity suspension flame spraying (HVSFS) coating process. To characterize the samples surface roughness, brightfield microscopy of the coatings, X-ray diffraction (XRD)-analysis for definition of the phase composition of the layers, Raman spectroscopy for determination of the phase composition of the contained metals, element-mapping for Cu-content verification, release kinetics for detection of metal ions and ceramic components of the coatings were carried out. The aim of this study was to evaluate in vitro biocompatibility and antimicrobial activity of the coatings. For biocompatibility testing, growth experiments were performed using the cell culture line MG-63. Cell viability was investigated by Giemsa staining and live/dead assay. The WST-1 kit was used to quantify cell proliferation and vitality in vitro and the lactate dehydrogenase (LDH) kit to quantify cytotoxicity. The formation of hydroxyapatite crystals in simulated body fluid was investigated to predict bioactivity in vivo. The Safe Airborne Antibacterial Assay with Staphylococcus aureus (S. aureus) was used for antimicrobial testing. The results showed good biocompatibility of all the metal doped CaP coatings, furthermore Cu and Ag doped layers showed significant antibacterial effects against S. aureus.

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Ultrastructural study of degradation of calcium phosphate ceramic by human monocytes and modulation of this activity by HILDA/LIF cytokine.

Journal of Histochemistry & Cytochemistry ◽

10.1177/44.10.8813078 ◽

1996 ◽

Vol 44 (10) ◽

pp. 1131-1140 ◽

Cited By ~ 15

Author(s):

M D Benahmed ◽

D Heymann ◽

M Berreur ◽

M Cottrel ◽

A Godard ◽

...

Keyword(s):

Calcium Phosphate ◽

Culture Medium ◽

Marked Decrease ◽

Calcium Phosphate Ceramic ◽

Multinuclear Cells ◽

Residual Bodies ◽

First Time

Biodegradation of ceramics in vivo is achieved essentially by monocytes and multinuclear cells (osteoclasts). Monocytes are the key element in this process because they intervene first at the biomaterial implantation site during inflammatory reaction. In this work, in vitro studies were conducted on an ultrastructural scale to determine the specific behavior of these cells with regard to a calcium phosphate (CaP) ceramic. Two types of phagocytosis were observed when cells came into contact with the biomaterial: either CaP crystals were taken up alone and then dissolved in the cytoplasm after disappearance of the phagosome membrane or they were incorporated together with large quantities of culture medium, in which case dissolution occurred after the formation of heterophagosomes. Phagocytosis of CaP coincided with autophagy and the accumulation of residual bodies in the cells. Addition of HILDA/LIF factor to these cultures induced a very marked decrease in phagocytotic activity directed at the capture of CaP crystals and culture medium. Autophagy was reduced, and residual bodies were rare or absent. This study specifies the role of monocytes in CaP biodegradation and demonstrates for the first time that HILDA/LIF has a biological effect on this cell line.

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Microwave assisted-in situ synthesis of porous titanium/calcium phosphate composites and their in vitro apatite-forming capability

Composites Part B Engineering ◽

10.1016/j.compositesb.2015.08.046 ◽

2015 ◽

Vol 83 ◽

pp. 50-57 ◽

Cited By ~ 20

Author(s):

Man-Tik Choy ◽

Chak-Yin Tang ◽

Ling Chen ◽

Wing-Cheung Law ◽

Chi-Pong Tsui ◽

...

Keyword(s):

Calcium Phosphate ◽

Porous Titanium ◽

In Situ Synthesis ◽

Microwave Assisted

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In-Vitro Dissolution Characteristics of Calcium Phosphate/Calcium Sulphate Based Hybrid Biomaterials

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.254-256.585 ◽

2003 ◽

Vol 254-256 ◽

pp. 585-588

Author(s):

C.P. Cleere ◽

G.M. Insley ◽

M.E. Murphy ◽

P.N. Maher ◽

A.M. Murphy

Keyword(s):

Calcium Phosphate ◽

Calcium Sulphate ◽

In Vitro Dissolution ◽

Hybrid Biomaterials

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Resorbable Synthetic Bone Grafts Formed From a Silicon Stabilized Calcium Phosphate Bioceramic

MRS Proceedings ◽

10.1557/proc-550-313 ◽

1998 ◽

Vol 550 ◽

Cited By ~ 2

Author(s):

S. Langstaff ◽

M. Sayer ◽

T. Smith ◽

S. Pugh

Keyword(s):

Calcium Phosphate ◽

Chemical Reactivity ◽

Calcium Hydroxyapatite ◽

Bone Grafts ◽

Ceramic Coatings ◽

Substitution Reactions ◽

Synthetic Bone ◽

Characteristic Features ◽

Synthetic Bone Grafts

AbstractSynthetic bone grafts resistant to random dissolution at physiological pH, yet capable of being gradually resorbed in vitro by osteoclasts have been created. Bulk ceramics and ceramic coatings formed from an additive stabilized colloidal sol possess two characteristic features: a phase mixture of calcium hydroxyapatite (HA) and a silicon stabilized tricalcium phosphate, and a microporous morphology based on inter-connected particles (0.2-1.0 μm). The characteristic phase composition arises during sintering through substitution reactions where silicon enters the calcium phosphate lattice under conditions of high chemical reactivity. Evidence for in vitro resorption lacunae on bulk ceramics is presented.

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Gene Expression of C2C12 Cells on Calcium Phosphate Ceramic In Vitro

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.288-289.265 ◽

2005 ◽

Vol 288-289 ◽

pp. 265-268 ◽

Cited By ~ 1

Author(s):

Yan Fei Tan ◽

Ling Li Zhang ◽

Xin Lai He ◽

Wei Qiang Xiao ◽

Hong Song Fan ◽

...

Keyword(s):

Calcium Phosphate ◽

Type I Collagen ◽

C2c12 Cell ◽

C2c12 Cells ◽

Type I ◽

Calcium Phosphate Ceramic ◽

Mtt Method ◽

Difference Expression

The osteoinduction of Calcium Phosphate (CaP) had been proved and generally been investigated by in vivo implantation. However, the mechanism of the osteoinductivity was not clear and it was difficult to judge the osteoinductivity in vitro. In this study, Mouse C2C12 cell line, a kind of myoblast precursor cell, was employed to co-culture with CaP. The induction of cell differentiation by materials was tested by MTT method, fluorescence observation, especially the mRNA expression of Osteocalcin, Type I collagen and Fibronectin by RT-PCR. It was founded that C2C12 cells could be induced to expression osteocalcin when growth on the surface of the HA/TCP ceramics. At the same time, the ceramics with different composition and sintering temperature seemed to induce difference expression level of the related genes. The results proved that phase composition was one of the most important factors in the regulation of bone-related genes. This study provided a potential model to evaluate the osteoinductivity of CaP ceramics in vitro.

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