scholarly journals Thin Degradable Coatings for Optimization of Osteointegration Associated with Simultaneous Infection Prophylaxis

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3495 ◽  
Author(s):  
Sophie Burtscher ◽  
Peter Krieg ◽  
Andreas Killinger ◽  
Ali Al-Ahmad ◽  
Michael Seidenstücker ◽  
...  
Keyword(s):  
Phase Composition ◽  
Calcium Phosphate ◽  
Release Kinetics ◽  
Titanium Substrate ◽  
Ceramic Coatings ◽  
Microbial Colonization ◽  
Flame Spraying ◽  
Infection Prophylaxis ◽  
Aseptic Prosthesis Loosening

One of the most common causes of implant failure is aseptic prosthesis loosening. Another frequent complication after prosthesis implant is the microbial colonization of the prosthesis surface, which often leads to a replacement of the prosthesis. One approach to reduce these complications is the application of bioactive substances to implant surfaces. Both an antibiotic prophylaxis and a faster osteointegration can be obtained by incorporation of bactericidal active metals in degradable calcium phosphate (CaP) coatings. In this study, thin degradable calcium phosphate ceramic coatings doped with silver (Ag), copper (Cu), and bismuth (Bi) on a titanium substrate were prepared with the aid of the high-velocity suspension flame spraying (HVSFS) coating process. To characterize the samples surface roughness, brightfield microscopy of the coatings, X-ray diffraction (XRD)-analysis for definition of the phase composition of the layers, Raman spectroscopy for determination of the phase composition of the contained metals, element-mapping for Cu-content verification, release kinetics for detection of metal ions and ceramic components of the coatings were carried out. The aim of this study was to evaluate in vitro biocompatibility and antimicrobial activity of the coatings. For biocompatibility testing, growth experiments were performed using the cell culture line MG-63. Cell viability was investigated by Giemsa staining and live/dead assay. The WST-1 kit was used to quantify cell proliferation and vitality in vitro and the lactate dehydrogenase (LDH) kit to quantify cytotoxicity. The formation of hydroxyapatite crystals in simulated body fluid was investigated to predict bioactivity in vivo. The Safe Airborne Antibacterial Assay with Staphylococcus aureus (S. aureus) was used for antimicrobial testing. The results showed good biocompatibility of all the metal doped CaP coatings, furthermore Cu and Ag doped layers showed significant antibacterial effects against S. aureus.

Resorbable Synthetic Bone Grafts Formed From a Silicon Stabilized Calcium Phosphate Bioceramic

1998 ◽  
Vol 550 ◽  
Author(s):  
S. Langstaff ◽  
M. Sayer ◽  
T. Smith ◽  
S. Pugh
Keyword(s):  
Calcium Phosphate ◽  
Chemical Reactivity ◽  
Calcium Hydroxyapatite ◽  
Bone Grafts ◽  
Ceramic Coatings ◽  
Substitution Reactions ◽  
Synthetic Bone ◽  
Characteristic Features ◽  
Synthetic Bone Grafts

AbstractSynthetic bone grafts resistant to random dissolution at physiological pH, yet capable of being gradually resorbed in vitro by osteoclasts have been created. Bulk ceramics and ceramic coatings formed from an additive stabilized colloidal sol possess two characteristic features: a phase mixture of calcium hydroxyapatite (HA) and a silicon stabilized tricalcium phosphate, and a microporous morphology based on inter-connected particles (0.2-1.0 μm). The characteristic phase composition arises during sintering through substitution reactions where silicon enters the calcium phosphate lattice under conditions of high chemical reactivity. Evidence for in vitro resorption lacunae on bulk ceramics is presented.

Thin Degradable Coatings for Optimization of Osteointegration Associated with Simultaneous Infection Prophylaxis

2016 ◽  
Vol 696 ◽  
pp. 171-176
Author(s):  
Anke Bernstein ◽  
Norbert Suedkamp ◽  
Hermann Otto Mayr ◽  
Rainer Gadow ◽  
Irina Arhire ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Cell Attachment ◽  
Calcium Phosphates ◽  
Thermal Spraying ◽  
In Vitro Cytotoxicity ◽  
Laser Scanning Microscopy ◽  
Cellular Growth ◽  
Flame Spraying ◽  
Infection Prophylaxis

The colonization of biomaterials with bacteria represents the main cause of implant-associated infections. Both an antibiotic prophylaxis and a faster osteointegration can be obtained by incorporation of bactericidal active metals in degradable CaP coatings. At present there is no reliable method on the basis of thermal spraying to get thin homogeneous layers containing silver, copper and bismuth in bacteriostatic / bactericidal concentrations. The aim of the study was the development and optimization of high-velocity suspension flame spraying (HVSFS) process for producing thin resorbable bioactive ceramics coatings on the basis of degradable calcium phosphates. In these layers the bacteriostatic / bactericidal effective metal copper should be integrated. Cells were grown on the materials for 3, 7, 14, and 21 days. Live/dead assay was used to measure cell viability. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay. Cell morphology, cell attachment, and cell spreading were investigated using laser scanning microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 21 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified

Investigations on Vitro Behaviour of Ceramic Coatings Deposited by High Velocity Suspension Flame Spraying

2011 ◽  
Vol 493-494 ◽  
pp. 530-534 ◽  
Author(s):  
Anke Bernstein ◽  
Norbert Suedkamp ◽  
Hermann Otto Mayr ◽  
R. Gadow ◽  
A. Killinger ◽  
...  
Keyword(s):  
High Velocity ◽  
Cell Attachment ◽  
Ceramic Coatings ◽  
In Vitro Cytotoxicity ◽  
Cellular Growth ◽  
Flame Spraying ◽  
Biological Fixation ◽  
Coating Materials ◽  
Identical Cell

Bioceramics used as coatings show different biocompatibility and bioactive behavior in relation to their chemical and morphological behavior. Bioactive ceramics such as β-tricalcium phosphate (β-TCP) promote and enhance biological fixation. Stable coatings require an optimum between resorption rate, flexural strength and adhesive strength of the coating. Therefore new bioceramic coating materials that ensure the balance between loss of substances and osteointegration need to be designed and investigated. By modifying the high velocity suspension flame spraying (HVSFS) process parameters, five coatings with different materials were obtained. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay after 24, 48 and 72 h. Cells were grown on the materials for 3, 7, 14, and 21 days and counted. Cell morphology, cell attachment, and cell spreading were investigated using fluorescence microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified.

Effects of Mg Additives on Properties of Mg-Doped Hydroxyapatite Ceramics

2010 ◽  
Vol 76 ◽  
pp. 60-65 ◽  
Author(s):  
Aneta Zima ◽  
Anna Ślósarczyk ◽  
Zofia Paszkiewicz ◽  
Magdalena Staszewska ◽  
Waldemar Mróz ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Phase Composition ◽  
Calcium Phosphate ◽  
Chemical Stability ◽  
Hydroxyapatite Ceramics ◽  
Biological Studies ◽  
Number Of Cells ◽  
Cells Cultured ◽  
Mg Doped

In the studies undoped HA and HA modified with 0.3; 0.6; 0.9; 1.8 wt % of Mg were prepared by the wet method. Introduction of magnesium into HA structure influenced its thermal stability as well as phase composition, sinterability, microstructure, flexural strength and chemical stability of the obtained calcium-phosphate ceramics. The presence of magnesium promoted the decomposition of HA to βTCP above 800°C. Beyond a certain limit (0.9 wt %), Mg ions caused formation of MgO in Mg-HA ceramics. Chemical stability of Mg modified HA below 0.9 wt % Mg under in vitro conditions was similar to that of the undoped hydroxyapatite. Biological studies showed that the number of cells cultured on the surface of HA samples with 1.8 wt % Mg additive, probably due to the MgO content, was lower than on the pure HA ceramics.

Interest of High Shear Wet Granulation to Produce Drug Loaded Porous Calcium Phosphate Pellets for Bone Filling

2008 ◽  
Vol 396-398 ◽  
pp. 535-538 ◽  
Author(s):  
Marylène Viana ◽  
Amelie Désiré ◽  
Emilie Chevalier ◽  
Eric Champion ◽  
Roxana Chotard-Ghodsnia ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Release Kinetics ◽  
Drug Loading ◽  
Wet Granulation ◽  
High Shear ◽  
Solvent Evaporation Method ◽  
In Vitro Drug Release ◽  
High Shear Wet Granulation ◽  
Bone Filling

Spherical porous calcium phosphate pellets were fabricated by high shear wet granulation using native starch as a binder. After a heat treatment to eliminate the organic template, pellets were loaded with ibuprofen by solvent evaporation method. In vitro drug release kinetics was determined using an USP II apparatus (Prolabo Dissolution Tester, France). Results showed the interest of the calcination and of the increase in the binder-porogen content, to improve the drug loading (higher drug content) and prolong the release of the drug substance.

Preparation and Characterization of Al2O3/ZrO2-MgO-CeO2 Composite Coatings Prepared by Flame Spraying

2011 ◽  
Vol 189-193 ◽  
pp. 410-415
Author(s):  
Wen Guang Zhao ◽  
Xin Zhi ◽  
Sheng Li An
Keyword(s):  
Phase Composition ◽  
Bond Strength ◽  
Composite Coatings ◽  
Ceramic Coatings ◽  
Flame Spraying ◽  
Precipitation Method ◽  
Flame Spray ◽  
X Ray Diffraction ◽  
The Difference ◽  
Co Precipitation

In the present work, ultra-fine Al2O3/ZrO2-MgO-CeO2powders were used as the starting materials to produce Al2O3-ZrO2composite coatings on 45# carbon steel by flame spray. The ultra-fine Al2O3/ZrO2-MgO-CeO2powders were prepared via the co-precipitation method accompanied a crushing sintering process. The microstructure, the phase compositions and the bond strength of the crushed sintered powders and the corresponding composite coatings were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD) and a tensile bond strength tests respectively. It was found that the phase composition, the surface topography and the coating adhesion were different from the difference kinds of coatings. Results indicated that the phase composition of the flame sprayed ceramic coatings consisted of tetragonal-ZrO2, cubic-ZrO2, ZTA (zirconia toughened alumina) and γ-Al2O3, with a small amount of α-Al2O3, and that bond strength values attained 45.1 MPa for 42.6wt.% Al2O3/57.4wt.% Mg0.13Ce0.1Zr0.77O1.87coating.

Bioactivity Assessment of Calcium Phosphate Coatings

2016 ◽  
Vol 720 ◽  
pp. 193-196
Author(s):  
Daniel Navarro da Rocha ◽  
Leila Rosa de Oliveira Cruz ◽  
Dindo Q. Mijares ◽  
Rubens Lincoln Santana Blazutti Marçal ◽  
José Brant de Campos ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Titanium Substrate ◽  
Chemical Deposition ◽  
Hydroxyapatite Coating ◽  
Deposition Method ◽  
Phosphate Coatings ◽  
Chemical Deposition Method ◽  
Calcium Phosphate Coatings

Nowadays, bioactive coatings or modifications on titanium surface have been tested in vitro and in vivo. In this study, two types of calcium phosphate coatings were produced by a chemical deposition method and their bioactivity assay in cell culture medium were investigated. The calcium phosphate coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy with field emission gun (FEG-SEM) analyses. Titanium substrate was successfully coated with brushite using chemical deposition method and, after a second step of conversion, the hydroxyapatite coating was obtained. The hydroxyapatite coating showed a bioactivity property after 14 days’ incubation in McCoy medium culture.

In Vitro Resorbability of 3D Printed Hydroxyapatite in Two Different pH Buffered Solutions

2019 ◽  
Vol 798 ◽  
pp. 71-76
Author(s):  
Faungchat Thammarakcharoen ◽  
Phee Palanuruksa ◽  
Jintamai Suwanprateeb
Keyword(s):  
Phase Composition ◽  
Calcium Phosphate ◽  
Water Solution ◽  
Sample Surface ◽  
Xrd Analysis ◽  
Octacalcium Phosphate ◽  
Deionized Water ◽  
Icp Oes ◽  
3D Printed

Resorability of 3D printed hydroxyapatite (3DP HA) in deionized water solution which was buffered with succinic acid-NaOH (pH 5.5) and Tris(hydroxymethyl aminomethane) (pH 7.4) for 1, 7, 14 and 28 days was carried out. Weight change and release of calcium (Ca) and phosphorus (P) were used to evaluate the sample resorption. It was found that the weight of samples soaking in both pH 5.5 and 7.4 solutions decreased with increasing soaking times, but the degree of decrease was greater at pH 5.5 than at pH 7.4. ICP-OES results showed that the release of Ca and P in both pH solutions increased with immersing times. The amount of Ca and P released at pH 5.5 was higher than at pH 7.4. Phase composition of the samples and the microstructure of the sample were characterized using XRD and SEM respectively. XRD analysis showed that hydroxyapatite (HA) and octacalcium phosphate (OCP) phases were found at the center of all samples, but the intensity of OCP peaks tended to decrease with increasing times. Only HA was found on the sample surface after immersion in both pH solutions at all soaking periods. After immersion, newly formed crystals were seen both at the center and/or on the surface of samples. These results suggested that pH could influence the resorption of the samples and also the formation of new calcium phosphate crystals.

Sign in / Sign up

Export Citation Format

Share Document