D.P13 In vitro antioxidant properties of astaxanthin on rainbow trout immune cells

Abstract Kynurenic acid (KYNA), an endogenous neuroprotectant formed along the kynurenine pathway of tryptophan degradation, is a selective ligand of the GPR35 receptor, which can be found on the surface of various populations of human immune cells. In infections and inflammations, KYNA produces an anti-inflammatory effect through this receptor, by depressing the synthesis of reactive oxygen species and pro-inflammatory cytokines. However, it is still unrecognized whether receptors for kynurenic acid are also localized on immune cells of poikilothermic animals, or whether KYNA is able to affect these cells. The objective of this study has been to determine the effect of different concentrations of kynurenic acid (12.5 μM to 10 mM) on the viability and mitogenic response of lymphocytes and on the activity of phagocytic cells isolated from blood and the spleen of rainbow trout. The results imply low toxicity of kynurenic acid towards fish immune cells, and the proliferative effect observed at the two lowest concentrations of KYNA (12.5 μM and 25 μM) seems indicative of endogenous kynurenic acid being capable of activating fish lymphocytes. Non-toxic, micromole concentrations of KYNA, however, had no influence on the mitogenic response of lymphocytes nor on the activity of phagocytes in rainbow trout under in vitro conditions. There is some likelihood that such an effect could be observed at lower, nanomole concentrations of KYNA.

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In Vitro and In Vivo Immunomodulator Activities of Allium sativum L.

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/4984659 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 9

Author(s):

Mouna Moutia ◽

Norddine Habti ◽

Abdallah Badou

Keyword(s):

Immune Cells ◽

Allium Sativum ◽

Therapeutic Potential ◽

Inflammatory Diseases ◽

Biological Activities ◽

Antioxidant Properties ◽

Allergic Airway Inflammation ◽

Cytokine Gene Expression

Allium Sativum L. (garlic), which is a species of the onion family, Alliaceae, is one of the most used plants in traditional medicine worldwide. More than 200 chemicals with diverse properties have been found in garlic extracts. Several garlic compounds were suggested to be efficient in improving various pathologies including certain types of cancer. This paper is an overview of data about garlic biological activities in vitro and/or in vivo on immune cells, on the development of certain inflammatory diseases, and on different types of carcinomas and sarcomas. Garlic and its compounds were found to have notable antioxidant properties. Garlic therapeutic potential has also been studied in several inflammatory diseases such as allergic-airway inflammation, inflammatory bowel disease, arthritic rheumatism, and atherosclerosis. Furthermore, garlic was found to be able to maintain the immune system homeostasis and to exhibit beneficial effects on immune cells especially through regulation of proliferation and cytokine gene expression. Finally, we will show how major garlic components such as sulfur compounds and polyphenols might be responsible for the garlic biological activities revealed in different situations. If identified, specific compounds present in garlic could potentially be used in therapy.

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Induction of foxp3 during the Crosstalk between Antigen Presenting Like-Cells MHCII+CD83+ and Splenocytes CD4+IgM− in Rainbow Trout

Biology ◽

10.3390/biology10040324 ◽

2021 ◽

Vol 10 (4) ◽

pp. 324

Author(s):

Byron Morales-Lange ◽

Ivan Nombela ◽

María Del Mar Ortega-Villaizán ◽

Mónica Imarai ◽

Paulina Schmitt ◽

...

Keyword(s):

Gene Expression ◽

Rainbow Trout ◽

Immune Cells ◽

Mhc Ii ◽

Different Types ◽

Piscirickettsia Salmonis ◽

Antigen Presenting ◽

Treg Phenotype ◽

Stimulation Of

In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Using these splenocytes, co-cultures of 24 and 48 h are used to determine the gene expression of master transcriptional factors that coordinate the polarization of T cells (t-bet, gata3, and foxp3). The results show a proportional upregulation of foxp3 (compared to t-bet and gata3) in co-cultures (at 24 h) of IFNγ-induced splenocytes with and without stimulation of Piscirickettsia salmonis proteins. In addition, foxp3 upregulation was established in co-cultures with IFNγ-induced cells and in cells only stimulated previously with P. salmonis proteins at 48 h of co-culture. These results show a potential communication between antigen-presenting-like cells and lymphocyte in the spleen, which could be induced towards a Treg phenotype.

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Eryngium caeruleum essential oil as a promising natural additive: in vitro antioxidant properties and its effect on lipid oxidation of minced rainbow trout meat during storage at refrigeration temperature

SSRN Electronic Journal ◽

10.2139/ssrn.3783481 ◽

2021 ◽

Author(s):

Sara Banikhademi ◽

Majid Aminzare ◽

Hassan Hassanzad Azar ◽

Mohammad Reza Mehrasbi

Keyword(s):

Rainbow Trout ◽

Essential Oil ◽

Lipid Oxidation ◽

Antioxidant Properties ◽

Natural Additive

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The Influence of Fucoxanthin Immobilized on Porous Aluminum-Silicon Carrier Surface on the Functional Activities of Immunocytes in Mice

KnE Life Sciences ◽

10.18502/kls.v7i1.10109 ◽

2022 ◽

Author(s):

Alexander Lykov ◽

Lubov Rachkovskaya ◽

Ruslan Gevorgiz ◽

Svetlana Zheleznova ◽

Olga Poveshchenko

Keyword(s):

Nitric Oxide ◽

Survival Rate ◽

Toxic Effect ◽

Immune Cells ◽

Antioxidant Properties ◽

Myeloperoxidase Activity ◽

Porous Aluminum ◽

Cylindrotheca Closterium ◽

Aluminum Silicon

Fucoxanthin is a natural carotenoid obtained from seaweed which exhibits antioxidant properties. This research aimed to assess whether fucoxanthin, immobilized on aluminum-silicon carrier particles, has a toxic effect on immune cells. The viability, proliferation, nitric oxide production and myeloperoxidase activity of thymocytes and splenocytes of mice in vitro were studied. It was shown that fucoxanthin, immobilized on aluminum-silicon carrier particles, increased the survival rate and proliferation of mature immunocytes (splenocytes) after 24 hours exposure and increased the survival rate of naïve immunocytes (thymocytes) when exposed for 120 hours. In terms of myeloperoxidase, the activity of the immune cells was not affected by fucoxanthin immobilized on the carrier particles. The obtained results indicated that fucoxanthin, immobilized on particles of an aluminum-silicon carrier, did not have a toxic effect on mouse immunocytes. Keywords: Cylindrotheca closterium, fucoxanthin, γ-aluminum oxide, polydimethylsiloxane, thymocytes, splenocytes, viability, proliferation, nitric oxide, myeloperoxidase activity

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Aryl Hydrocarbon Receptor Signaling Is Functional in Immune Cells of Rainbow Trout (Oncorhynchus mykiss)

International Journal of Molecular Sciences ◽

10.3390/ijms21176323 ◽

2020 ◽

Vol 21 (17) ◽

pp. 6323

Author(s):

Jun-Young Song ◽

Ayako Casanova-Nakayama ◽

Anja-Maria Möller ◽

Shin-Ichi Kitamura ◽

Kei Nakayama ◽

...

Keyword(s):

Immune System ◽

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Aromatic Hydrocarbons ◽

Immune Cells ◽

Polynuclear Aromatic Hydrocarbons ◽

Diverse Range ◽

Rainbow Trout Oncorhynchus Mykiss

The arylhydrocarbon receptor (AhR) is an important signaling pathway in the immune system of mammals. In addition to its physiological functions, the receptor mediates the immunotoxic actions of a diverse range of environmental contaminants that bind to and activate the AhR, including planar halogenated aromatic hydrocarbons (PHAHs or dioxin-like compounds) and polynuclear aromatic hydrocarbons (PAHs). AhR-binding xenobiotics are immunotoxic not only to mammals but to teleost fish as well. To date, however, it is unknown if the AhR pathway is active in the immune system of fish and thus may act as molecular initiating event in the immunotoxicity of AhR-binding xenobiotics to fish. The present study aims to examine the presence of functional AhR signaling in immune cells of rainbow trout (Oncorhynchus mykiss). Focus is given to the toxicologically relevant AhR2 clade. By means of RT-qPCR and in situ hybdridization, we show that immune cells of rainbow trout express ahr 2α and ahr 2β mRNA; this applies for immune cells isolated from the head kidney and from the peripheral blood. Furthermore, we show that in vivo as well as in vitro exposure to the AhR ligand, benzo(a)pyrene (BaP), causes upregulation of the AhR-regulated gene, cytochrome p4501a, in rainbow trout immune cells, and that this induction is inhibited by co-treatment with an AhR antagonist. Taken together, these findings provide evidence that functional AhR signaling exists in the immune cells of the teleost species, rainbow trout.

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In vitro mercury-related cytotoxicity and functional impairment of the immune cells of rainbow trout (Oncorhynchus mykiss)

Aquatic Toxicology ◽

10.1016/0166-445x(94)90046-9 ◽

1994 ◽

Vol 29 (1-2) ◽

pp. 37-48 ◽

Cited By ~ 33

Author(s):

Isabelle Voccia ◽

Krzysztof Krzystyniak ◽

Muriel Dunier ◽

Denis Flipo ◽

Michel Fournier

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Functional Impairment ◽

Immune Cells ◽

Rainbow Trout Oncorhynchus Mykiss

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QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp350-354 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

GITA MISHRA ◽

HEMESHWER KUMAR CHANDRA ◽

NISHA SAHU ◽

SATENDRA KUMAR NIRALA ◽

MONIKA BHADAURIA

Keyword(s):

Reducing Sugars ◽

Antioxidant Properties ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Qualitative And Quantitative ◽

The Family ◽

Ethanolic Extracts ◽

Antioxidant Evaluation ◽

In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

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