Control of G1 to S cell cycle progression of Trypanosoma brucei S427c11 organisms under axenic conditions

AbstractPreviously we demonstrated that an excess of poly (ADP-ribose) in the nucleus makes procyclic parasites more sensitive to hydrogen peroxide. However, the effect of an altered-PAR metabolism under standard conditions has not been addressed yet. Here we have analyzed the behavior of the growth curve of transgenic parasites that present this phenotype and studied cell cycle progression in synchronized cultures by flow cytometry and immunofluorescence. We have demonstrated that an excess of nuclear poly (ADP-ribose) produces a delay in the G1 phase of the cell cycle. Moreover, for the first time we have shown that poly (ADP-ribose) occurs at specific points very close to the mature basal body, suggesting there could be a link between the kinetoplast and poly (ADP-ribose) metabolism.

Download Full-text

Role of Trypanosoma brucei Tim17 during cell survival and cell cycle progression

The FASEB Journal ◽

10.1096/fasebj.20.4.a493 ◽

2006 ◽

Vol 20 (4) ◽

Author(s):

Emmanuel K Peprah ◽

Minu Chaudhuri

Keyword(s):

Cell Cycle ◽

Cell Survival ◽

Trypanosoma Brucei ◽

Cell Cycle Progression ◽

Cycle Progression

Download Full-text

Regulators of Trypanosoma brucei Cell Cycle Progression and Differentiation Identified Using a Kinome-Wide RNAi Screen

PLoS Pathogens ◽

10.1371/journal.ppat.1003886 ◽

2014 ◽

Vol 10 (1) ◽

pp. e1003886 ◽

Cited By ~ 100

Author(s):

Nathaniel G. Jones ◽

Elizabeth B. Thomas ◽

Elaine Brown ◽

Nicholas J. Dickens ◽

Tansy C. Hammarton ◽

...

Keyword(s):

Cell Cycle ◽

Trypanosoma Brucei ◽

Cell Cycle Progression ◽

Rnai Screen ◽

Cycle Progression

Download Full-text

Maintaining the protective variant surface glycoprotein coat of African trypanosomes

Biochemical Society Transactions ◽

10.1042/bst0330981 ◽

2005 ◽

Vol 33 (5) ◽

pp. 981-982 ◽

Cited By ~ 1

Author(s):

G. Rudenko

Keyword(s):

Cell Cycle ◽

Trypanosoma Brucei ◽

Chronic Infection ◽

Cell Cycle Progression ◽

Surface Glycoprotein ◽

Variant Surface Glycoprotein ◽

Cycle Progression ◽

African Trypanosomes ◽

Immune Attack ◽

Protective Variant

The African trypanosome Trypanosoma brucei has a precarious existence as an extracellular parasite of the mammalian bloodstream, where it is faced with continuous immune attack. Key to survival is a dense VSG (variant surface glycoprotein) coat, which is repeatedly switched during the course of a chronic infection. New data demonstrate a link between VSG synthesis and cell cycle progression, indicating that VSG is monitored during the trypanosome cell cycle.

Download Full-text

Single-cell transcriptomic analysis of bloodstream Trypanosoma brucei reconstructs cell cycle progression and developmental quorum sensing

Nature Communications ◽

10.1038/s41467-021-25607-2 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Emma M. Briggs ◽

Federico Rojas ◽

Richard McCulloch ◽

Keith R. Matthews ◽

Thomas D. Otto

Keyword(s):

Cell Cycle ◽

Single Cell ◽

Trypanosoma Brucei ◽

Cell Cycle Progression ◽

Expression Patterns ◽

Tsetse Fly ◽

Cycle Progression ◽

Progressive Development ◽

Asynchronous Development

AbstractDevelopmental steps in the trypanosome life-cycle involve transition between replicative and non-replicative forms specialised for survival in, and transmission between, mammalian and tsetse fly hosts. Here, using oligopeptide-induced differentiation in vitro, we model the progressive development of replicative ‘slender’ to transmissible ‘stumpy’ bloodstream form Trypanosoma brucei and capture the transcriptomes of 8,599 parasites using single cell transcriptomics (scRNA-seq). Using this framework, we detail the relative order of biological events during asynchronous development, profile dynamic gene expression patterns and identify putative regulators. We additionally map the cell cycle of proliferating parasites and position stumpy cell-cycle exit at early G1 before progression to a distinct G0 state. A null mutant for one transiently elevated developmental regulator, ZC3H20 is further analysed by scRNA-seq, identifying its point of failure in the developmental atlas. This approach provides a paradigm for the dissection of differentiation events in parasites, relevant to diverse transitions in pathogen biology.

Download Full-text