Effect of Phytophthora infestans infection on superoxide dismutase dependent cytochrome c—reducing activities of leaves as related to resistance of potato plants to late blight

Plant Science ◽  
1991 ◽  
Vol 78 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Diana G. Ivanova ◽  
Nina V. Gughova ◽  
Mark N. Merzlyak ◽  
Galina V. Rassadina
Keyword(s):  
Superoxide Dismutase ◽  
Cytochrome C ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Plants

scholarly journals Development of Real-Time Isothermal Amplification Assays for On-Site Detection of Phytophthora infestans in Potato Leaves

Plant Disease ◽  
2017 ◽  
Vol 101 (7) ◽  
pp. 1269-1277 ◽  
Author(s):  
Mélissa Si Ammour ◽  
Guillaume J. Bilodeau ◽  
David Mathieu Tremblay ◽  
Hervé Van der Heyden ◽  
Thaer Yaseen ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Real Time ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Closely Related Species ◽  
Potato Plants ◽  
Crude Plant Extract ◽  
Time Loop ◽  
Potato Pathogens

Real-time loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) assays were developed targeting the internal transcribed spacer 2 region of the ribosomal DNA of Phytophthora infestans, the potato late blight causal agent. A rapid crude plant extract (CPE) preparation method from infected potato leaves was developed for on-site testing. The assay’s specificity was tested using several species of Phytophthora and other potato fungal and oomycete pathogens. Both LAMP and RPA assays showed specificity to P. infestans but also to the closely related species P. andina, P. mirabilis, P. phaseoli, and P. ipomoeae, although the latter are not reported as potato pathogen species. No cross-reaction occurred with P. capsici or with the potato pathogens tested, including P. nicotianae and P. erythroseptica. The sensitivity was determined using P. infestans pure genomic DNA added into healthy CPE samples. Both LAMP and RPA assays detected DNA at 50 fg/μl and were insensitive to CPE inhibition. The isothermal assays were tested with artificially inoculated and naturally infected potato plants using a Smart-DART platform. The LAMP assay effectively detected P. infestans in symptomless potato leaves as soon as 24 h postinoculation. A rapid and accurate on-site detection of P. infestans in plant material using the LAMP assay will contribute to improved late blight diagnosis and early detection of infections and facilitate prompt management decisions.

scholarly journals Arabidopsis late blight: Infection of a nonhost plant by Albugo laibachii enables full colonization by Phytophthora infestans

2015 ◽  
Author(s):  
Khaoula Belhaj ◽  
Liliana M. Cano ◽  
David C. Prince ◽  
Ariane Kemen ◽  
Kentaro Yoshida ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Molecular Mechanisms ◽  
Transcriptional Profiling ◽  
Unexpected Finding ◽  
Potato Plants ◽  
Nonhost Plant ◽  
Gene Expression Dynamics ◽  
Oomycete Pathogen ◽  
Arabidopsis Cells

AbstractThe oomycete pathogen Phytophthora infestans causes potato late blight, and as a potato and tomato specialist pathogen, is seemingly poorly adapted to infect plants outside the Solanaceae. Here, we report the unexpected finding that P. infestans can infect Arabidopsis thaliana when another oomycete pathogen, Albugo laibachii, has colonized the host plant. The behaviour and speed of P. infestans infection in Arabidopsis pre-infected with A. laibachii resemble P. infestans infection of susceptible potato plants. Transcriptional profiling of P. infestans genes during infection revealed a significant overlap in the sets of secreted-protein genes that are induced in P. infestans upon colonisation of potato and susceptible Arabidopsis, suggesting major similarities in P. infestans gene expression dynamics on the two plant species. Furthermore, we found haustoria of A. laibachii and P. infestans within the same Arabidopsis cells. This Arabidopsis - A. laibachii - P. infestans tripartite interaction opens up various possibilities to dissect the molecular mechanisms of P. infestans infection and the processes occurring in co-infected Arabidopsis cells.

Resistance to late blight (Phytophthora infestans (Mont.) de Bary) of potato plants regenerated fromin vitro selected calli

1993 ◽  
Vol 36 (4) ◽  
pp. 341-351 ◽  
Author(s):  
C. Cerato ◽  
L. M. Manici ◽  
S. Borgatti ◽  
R. Alicchio ◽  
R. Ghedini ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Plants

scholarly journals New Markers for Potato Late Blight Resistance and Susceptibility Using FTIR Spectroscopy

2012 ◽  
Vol 40 (1) ◽  
pp. 150 ◽  
Author(s):  
Abdelmoumen TAOUTAOU ◽  
Carmen SOCACIU ◽  
Doru PAMFIL ◽  
Florinela FETEA ◽  
Erika BALAZS ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Fourier Transform Infrared Spectroscopy ◽  
Late Blight Resistance ◽  
Fourier Transform Infrared ◽  
Potato Plants ◽  
Potato Resistance ◽  
Potato Crops

The potato (Solanum tuberosum) is the 3rd most important crop worldwide, and Phytophthora infestans is the most devastating pathogen to potato crops. In this study, it has identified markers for resistance and susceptibility to late blight using potato genotypes that differ in their resistance/susceptibility to Phytophthora infestans. Using Fourier transform infrared spectroscopy, many absorbance bands have been identified as specific to resistant potato plants, and others were specific to susceptible potato plants. For each case (resistance/susceptibility), three bands were identified: 941-1180 cm-1, 1336-1483 cm-1, and 1483-1703 cm-1 and 1056-1294 cm-1, 1442-1585 cm-1, and 1585-1832 cm-1, respectively. As it is a simple, rapid, and inexpensive technology, FTIR (Fourier Transform Infrared) spectroscopy offers an excellent opportunity for studying potato resistance/susceptibility to late blight.

scholarly journals Preemergence Infection of Potato Sprouts by Phytophthora infestans in the Highland Tropics of Ecuador

Plant Disease ◽  
2008 ◽  
Vol 92 (4) ◽  
pp. 569-574 ◽  
Author(s):  
Peter Kromann ◽  
Arturo Taipe ◽  
Jorge L. Andrade-Piedra ◽  
Lisa Munk ◽  
Gregory A. Forbes
Keyword(s):  
Disease Management ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Seed Potato ◽  
Soil Surface ◽  
Potato Tubers ◽  
Potato Plants ◽  
Management Approaches

Experiments were conducted to determine whether preemergence infection of potato sprouts by Phytophthora infestans occurs in the highland tropics of Ecuador. In three separate experiments in the field, P. infestans was identified on the preemerged sprouts of 49, 5, and 43% of tubers, respectively, which had been removed from soil prior to emergence. Tubers had been planted within 10 m of approximately 300-m2 plots with mature potato plants severely infected with late blight. Infection potential of potato sprouts also was evaluated in the greenhouse by applying 10-ml sporangial suspensions (50 and 250 sporangia/ml) daily for 10 days to the soil surface of pots planted with sprouted seed potato tubers. The daily inoculation rate of 50 sporangia/ml (15.9 × 103 sporangia/m2) resulted in sprout infection in 100% of inoculated pots and roughly corresponded to the sporangial deposition accumulated over 24 h in the field. Deposition had been measured at 1 m from a severely infected potato plot. Our study demonstrated the potential for preemergence infection of potato sprouts by P. infestans in the highlands of Ecuador, where year-round aerial inoculum is present. Preemergence infection is consistent with high levels of disease sometimes seen in recently emerged potato fields. These experiments indicate a need to reconsider disease management approaches.

scholarly journals Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1115
Author(s):  
Antonina Sorokan ◽  
Galina Benkovskaya ◽  
Guzel Burkhanova ◽  
Darya Blagova ◽  
Igor Maksimov
Keyword(s):  
Bacillus Subtilis ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Leptinotarsa Decemlineata ◽  
Systemic Resistance ◽  
Pr Genes ◽  
Mycelium Growth ◽  
Modern Approach ◽  
Potato Plants ◽  
Leptinotarsa Decemlineata Say

Novel properties of a previously obtained Bacillus subtilis 26DCryChS strain are described. The B. subtilis 26DCryChS strain is able to produce Cry1Ia δ-endotoxin from B. thuringiensis B-5351 and to exist in internal plant tissues of potato plants in the same manner as the endophytic B. subtilis 26D source strain (487 ± 53 and 420 ± 63 CFU*103/g, respectively). B. subtilis 26DCryChS, as much as the original B. subtilis 26D strain, inhibited mycelium growth of oomycete Phytophthora infestans (Mont.) de Bary and reduced late blight symptoms development on plants by 35% compared with non-treated ones, as well as showed insecticidal activity against Leptinotarsa decemlineata. Production of the fluorescent GFP protein in the B. subtilis 26D genome allowed visualizing the endophytes around damaged sites on beetle intestines. Bacillus strains under investigation induced systemic resistance to P. infestans and L. decemlineata through the activation of the transcription of PR genes in potato plants. Thus, the B. subtilis 26DCryChS strain was able to induce transcription of jasmonate-dependent genes and acquired the ability to promote transcription of a salicylate-dependent gene (PR1) in plants infected with the late blight agent and damaged by Colorado potato beetle larvae. The B. subtilis 26DCryChS strain could be put forward as a modern approach for biocontrol agents design.

scholarly journals INFLUENCE OF BACILLUS SUBTILIS 26D ON GROWTH PARAMETERS AND IAA CONTENT IN POTATO PLANTS INFECTED WITH PHYTOPHTHORA INFESTANS

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (2) ◽  
pp. 89-93
Author(s):  
A.V. Sorokan ◽  
◽  
S.V. Veselova ◽  
I.V. Maksimov ◽  
◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Growth Parameters ◽  
Dry Mass ◽  
Plant Diseases ◽  
Negative Effects ◽  
Potato Plants ◽  
Endophytic Microorganisms ◽  
Regulation Of Growth

Plant diseases, including late blight caused by Phytophthora infestans, have long-term negative effects on plant growth and productivity. Auxins play an important role both in the regulation of growth processes and in the interaction of plants with pathogenic and endophytic microorganisms. It was shown that the presence of endophytic bacteria B. subtilis 26D in potato tissues promoted a rapid recovery of the growth rates of shoots, wet and dry mass of roots after P. infestans infection, and also reduced the area of late blight damaged zones on the leaves. At the same time, the effect of the pathogen led to an increase in the IAA content in the shoots, which was not observed in infected plants containing B. subtilis 26D endophytic cells.

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