Identification and antibiotic sensitivity of microbial contaminants from callus cultures of garlic Allium sativum L. and Allium longicuspis Regei

Plant Science ◽  
1996 ◽  
Vol 113 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Martin Fellner ◽  
Wolfgang Kneifel ◽  
Dorothea Gregorits ◽  
Wolfgang Leonhardt
Keyword(s):  
Allium Sativum ◽  
Antibiotic Sensitivity ◽  
Callus Cultures ◽  
Microbial Contaminants ◽  
Allium Longicuspis

scholarly journals Microbial Contaminants of Cord Blood Units Identified by 16S rRNA Sequencing and by API Test System, and Antibiotic Sensitivity Profiling

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141152 ◽  
Author(s):  
Luís França ◽  
Catarina Simões ◽  
Marco Taborda ◽  
Catarina Diogo ◽  
Milton S. da Costa
Keyword(s):  
16S Rrna ◽  
Cord Blood ◽  
Antibiotic Sensitivity ◽  
Test System ◽  
16S Rrna Sequencing ◽  
Rrna Sequencing ◽  
Microbial Contaminants

scholarly journals Genetic Diversity among U.S. Garlic Clones as Detected Using AFLP Methods

2004 ◽  
Vol 129 (4) ◽  
pp. 559-569 ◽  
Author(s):  
Gayle M. Volk ◽  
Adam D. Henk ◽  
Christopher M. Richards
Keyword(s):  
Allium Sativum ◽  
Fragment Length Polymorphism ◽  
Cultural Practices ◽  
Aflp Analysis ◽  
Cultivation Conditions ◽  
National Plant Germplasm System ◽  
Commercial Sources ◽  
Allium Longicuspis ◽  
High Degree ◽  
Selection Of

Garlic (Allium sativum L.) has been clonally propagated for thousands of years because it does not produce seed under standard cultivation conditions. A single garlic accession frequently displays a high degree of phenotypic plasticity that is likely to be dependent upon soil type, moisture, latitude, altitude, and cultural practices. The diversity observed by collectors has occasionally led to the renaming of varieties as they are exchanged among growers and gardeners. As a result, there are numerous garlic varieties available both commercially and within the USDA National Plant Germplasm System (NPGS) that may be identical genotypically, yet have unique cultivar names. To address this possibility, we performed amplified fragment-length polymorphism (AFLP) analysis on a comprehensive selection of 211 Allium sativum and Allium longicuspis accessions from NPGS and commercial sources. We used several statistical approaches to evaluate how these clonal lineages are genetically differentiated and how these patterns of differentiation correspond to recognized phenotypic classifications. These data suggest that while there are extensive duplications within the surveyed accessions, parsimony and distance based analyses reveal substantial diversity that is largely consistent with major phenotypic classes.

scholarly journals Classification of Genetic Variation in Garlic (Allium sativum L.) Revealed by RAPD

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1102-1104 ◽  
Author(s):  
M. Al-Zahim ◽  
H.J. Newbury ◽  
B.V. Ford-Lloyd
Keyword(s):  
Cluster Analysis ◽  
Genetic Variation ◽  
Morphological Variation ◽  
Allium Sativum ◽  
Rapd Analysis ◽  
Wild Progenitor ◽  
Taxonomic Affinity ◽  
Oligonucleotide Primers ◽  
Allium Longicuspis

RAPD analysis was employed to assess genetic variation in named cultivars of garlic (Allium sativum L.), and to examine the relationships between cultivated garlic varieties and the wild progenitor Allium longicuspis. Twenty-seven accessions were subjected to RAPD analysis using 26 oligonucleotide primers. Of a total of 292 bands, 63 (21%) were polymorphic. Cluster analysis revealed groupings that in part reflected patterns of morphological variation. All bolting forms (including wild and cultivated) grouped separately from the nonbolting cultivars. A. longicuspis and var. ophioscorodon grouped together, indicating close taxonomic affinity. Based upon relative levels of variation within different groups, we suggest potential relationships within the A. sativum/A. longicuspis complex.

scholarly journals (8) Summary of Fertility Characteristics of the USDA Garlic Collection When Grown in Pullman, Washington

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1039B-1039
Author(s):  
Barbara C. Hellier ◽  
Marie Pavelka
Keyword(s):  
Seed Production ◽  
Flower Development ◽  
Allium Sativum ◽  
Pollen Viability ◽  
Flower Color ◽  
Plant Introduction ◽  
Pollen Production ◽  
Viable Pollen ◽  
Western Regional ◽  
Allium Longicuspis

The USDA garlic (Allium sativum and Allium longicuspis) collection is maintained at the ARS, Western Regional Plant Introduction Station (WRPIS) in Pullman, Wash. This collection comprises 269 accessions, of which 153 are hardneck (flower producing) types. The fertility characteristics of these accessions was evaluated in the field at Pullman, Wash. After the spathes opened, bulbils were removed from all the evaluated accessions to facilitate flower development. The umbel and flower characteristics taken were anther color, flower color, flower shape, stigma position, flowers per umbel, umbel diameter, umbel shape, umbel defects, bulbil size, bulbil color, ease of bulbil removal, spathe opening, pollen production, and pollen viability. Of the 153 accessions, 10 produced only partial scapes with bulbils midstalk and no seed production capability. Viable pollen was shed in 85 accessions with viability ranging from 8% to 85%. Open-pollinated seed was generated by 19 of the Pullman, Wash., grown accessions. Seed production was low with yields from 6 to 91 seeds per accession.

scholarly journals Microbial Contaminants in Fresh and Extended Turkey Semen and their Sensitivity to Antibiotics

2021 ◽  
Vol 41 (1) ◽  
pp. 1-6
Author(s):  
I.M Alkali ◽  
S.O. Asuku ◽  
M.B. Umar ◽  
A. Abba ◽  
A. Mustapha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Key Words ◽  
Nalidixic Acid ◽  
Enterococcus Faecalis ◽  
Antibiotic Sensitivity ◽  
Microbial Culture ◽  
Microbial Contaminants ◽  
Semen Extender ◽  
Culture Identification

Microorganisms that inhabit the avian cloaca usually contaminate poultry semen which could easily spread throughout an entire flock. This study was conducted to determine the presence of microbial contaminants in turkey semen and evaluate their antibiotic sensitivity. Semen was collected from each tom, pooled and then divided into two aliquots A and B. Aliquot A was immediately  evaluated for microbial contaminants and antibiotic sensitivity while aliquot B, was extended and preserved for 24 hours at 40 C and thereafter microbial culture, identification and antibiotic sensitivity were conducted. Escherichia coli, Enterococcus faecalis, Bacillus subtilis, Corynebacteria species and a fungal organism Candida albican were isolated and identified in both aliquots. All the identified organisms were sensitive to pefloxacin, gentamicin and ciprofloxacin, while Enterococcus faecalis, Bacillus subtilis and Corynebacteria species were resistant to Ampicillin-cloxacillin, cefuroxime, amoxicillin and ceftriaxone. Escherichia coli was only resistant to co-trimoxazole, ofloxacin and nalidixic acid. The study concludes that, Escherichia coli, Enterococcus foecalis, Bacillus subtilis, Corynebacteria species and Candida albican were found to be turkey semen contaminants and were resistant to penicillin and streptomycin combination in turkey semen extender but sensitive to pefloxacin, gentamicin and ciprofloxacin. Key words: Microbial contaminants, turkey semen, extender, antibiotic sensitivity

scholarly journals Efficient Plant Regeneration from Callus Cultures in Garlic (Allium sativum L.).

1995 ◽  
Vol 12 (3) ◽  
pp. 259-265
Author(s):  
Kenji SATO ◽  
Takeshi ENDO ◽  
Tomoko KUSAYANAGI ◽  
Jun-ichi SHIGETA
Keyword(s):  
Plant Regeneration ◽  
Allium Sativum ◽  
Callus Cultures
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