Determination of the alleles of HLA I class in Georgian, Kurdish and Czech populations by ARMS-PCR method

1996 ◽  
Vol 47 (1-2) ◽  
pp. 54
Author(s):  
Bendukidze Nina ◽  
Meschishvili Elena ◽  
Ivašková Eva ◽  
Černá Marie ◽  
Churadze Tamaz ◽  
...  
Keyword(s):  
Arms Pcr ◽  
Pcr Method

scholarly journals Exon 9 LEPR Gene SNP Polymorphism of Hybrid Chickens F2Kambro Crossbreeds of ♀ F1Kambro with ♂ F1Kambro

2021 ◽  
Author(s):  
I Wayan Swarautama Mahardhika ◽  
Budi Setiadi Daryono
Keyword(s):  
Nucleotide Polymorphisms ◽  
Arms Pcr ◽  
Specific Protocol ◽  
Correlation Degree ◽  
Transversion Mutation ◽  
Gene Correlation ◽  
Exon 9 ◽  
Pcr Method ◽  
Dna Template

AbstractThe implementation of the T-ARMS PCR method in the detection of single nucleotide polymorphisms (SNPs) in the LEPR gene in chicken DNA samples has never been conducted. This research aims to design a specific protocol for exon 9 LEPR gene SNPs detection and detect LEPR gene expression or LEPR SNPs in Pelung chicken samples, F1Pelung, Layer, Broiler Cobb 500, F1Kambro chicken and F2Kambro chicken using the T-ARMS PCR method. Determination of LEPR gene correlation degree on Body Weight (BT) and Egg Productivity (PT) in F1 Kambro population and F2Kambro. Qualitative phenotype parameters showed six groups of segregated phenotypes compared to F1Kambro chicken. Growth of F2Kambro chicken weight reached 753.36 ± 155.31 grams in 8 weeks was not significant for F1Kambro chicken due to inbreeding depression (Fx = 25%, IR = 4.925%) and transversion of A LEPR allele mutations. Specific protocol detection of exon 9 LEPR gene SNPs using the T-ARMS PCR method can detect C127A LEPR mutations with IP: OP ratio 10:1 pmol / µM, chicken DNA template concentration of 100 ng / µL with annealing temperature of 55.7° C / 30s. The transversion mutation of C127A of LEPR exon 9 SNP were detected in DNA samples of F1Kambro hens (80%), F2Kambro roosters (20%), Broiler Cobb 500 hens (75%). The mutations were not detected in Layer, Pelung Blirik Hitam chicken and F1Pelung populations.

scholarly journals A rapid and cost‐effective multiplex ARMS‐PCR method for the simultaneous genotyping of the circulating SARS‐CoV‐2 phylogenetic clades

2021 ◽  
Author(s):  
Mohammad Tanvir Islam ◽  
ASM Rubayet Ul Alam ◽  
Najmuj Sakib ◽  
Mohammad Shazid Hasan ◽  
Tanay Chakrovarty ◽  
...  
Keyword(s):  
Cost Effective ◽  
Arms Pcr ◽  
Pcr Method

scholarly journals Identification of the Trichinella species by PCR method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 41-47
Author(s):  
Sasa Vasilev ◽  
Jelena Cvetkovic ◽  
Ivana Radovic ◽  
Ljiljana Sofronic-Milosavljevic
Keyword(s):  
Human Health ◽  
Health Problem ◽  
Continuous Monitoring ◽  
Human Consumption ◽  
Sylvatic Cycle ◽  
Trichinella Infection ◽  
High Prevalence ◽  
Pcr Method ◽  
Pig Meat

Serbia is country with a high prevalence of Trichinella infection in pigs, which continues to be a serious human health problem. In Serbia, only a few isolates of Trichinella found in pork have been genetically specified to date, and all were proven as T. spiralis. New data shows that in the sylvatic cycle in Serbia, at least in the Belgrade district, more than one Trichinella species co-exist (T. spiralis and T. britovi). Increased awareness of the possible overlap among sylvatic and domestic Trichinella cycles indicates the need for continuous monitoring of Trichinella species circulation and strongly points to the need that all isolates of Trichinella found in meat for human consumption should be subject to a determination of the Trichinella species (due to the risk of transmission of infection with T. britovi to domestic pigs and humans). This is why we examined using PCR the Trichinella larvae found in pig meat that caused a human outbreak (Trichinella infection) in Grocka (Belgrade district) during February 2011. The isolated larvae belonged to T. spiralis.

scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

2008 ◽  
Vol 24 (1-2) ◽  
pp. 117-126
Author(s):  
Vladimir Zimmermann ◽  
V. Kulísek ◽  
A. Copík ◽  
M. Odstrcil ◽  
Ondrej Debrecéni ◽  
...  
Keyword(s):  
Rectus Femoris ◽  
Muscle Fibres ◽  
Fat Cells ◽  
Average Thickness ◽  
Triceps Brachii ◽  
Surface Representation ◽  
Histochemical Analysis ◽  
Pcr Method ◽  
Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

Identication of HLA-B* and HLA-B*45 alleles using a nested ARMS-PCR method

1996 ◽  
Vol 47 (1-2) ◽  
pp. 9 ◽  
Author(s):  
Maria De Luca ◽  
Jonathan H Moses ◽  
Steven GE Marsh ◽  
Julia G Bodmer
Keyword(s):  
Arms Pcr ◽  
Pcr Method

Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method

2018 ◽  
Vol 44 (2) ◽  
pp. 217-224 ◽  
Author(s):  
M. A. Simonova ◽  
V. D. Pivovarov ◽  
D. Yu. Ryazantsev ◽  
M. A. Kostromina ◽  
T. I. Muravieva ◽  
...  
Keyword(s):  
Specific Class ◽  
Bet V 1 ◽  
Pcr Method ◽  
Class E

scholarly journals A new method for rapid detection of Listeria monocytogenes in food

2000 ◽  
Vol 18 (No. 3) ◽  
pp. 103-109 ◽  
Author(s):  
K. Zdeňková ◽  
K. Demnerová ◽  
G. Jeníková ◽  
J. Pazlarová
Keyword(s):  
Listeria Monocytogenes ◽  
Pcr Primers ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Special Importance ◽  
Ground Meat ◽  
Specific Detection ◽  
Gene Encoding ◽  
Pcr Method

Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which represents its main means of entry into the organism, is a topic of special importance. The original classic methods for the determination of Listeria monocytogenes are in general laborious and time-consuming procedures. In order to address this issue we developed a new rapid method for specific detection of Listeria monocytogenes in food samples. The method consists of three steps: i) enrichment of food microflora (16 h), ii) selective isolation of Listeria sp. exploiting immunomagnetic separation (2–3 h) followed by iii) precise identification of Listeria sp. and Listeria monocytogenes using duplex PCR. PCR primers specific to part of 16S rRNA were used in order to identify the members of Listeria genus. The specific identification of Listeria monocytogenes was accomplished exploiting a pair of primers specific to gene encoding invasion-associated protein – iap (4–5 h). Amplification products, 1003 bp and 593 bp respectively, were separated by electrophoresis and visualized by UV detection. The optimized IMS-PCR method was used to test the presence of Listeria sp. and Listeria monocytogenes in food samples (ground meat, low-fat milk and cheese [olomoucké tvarůžky]). A comparison of the efficiency of the bacteria enrichment step by IMS and centrifugation was also performed. The analysis time including enrichment is less than 24 h. The detection limit for Listeria monocytogenes was found between 101–102 cfu per 25 g of food sample.

scholarly journals Species level identification of thermotolerant campylobacters 

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 543-547 ◽  
Author(s):  
I. Kolackova ◽  
R. Karpiskova
Keyword(s):  
Species Identification ◽  
Mixed Cultures ◽  
Species Determination ◽  
The Czech Republic ◽  
Identification Process ◽  
Pcr Method ◽  
Phenotypic Methods ◽  
Level Identification ◽  
Campylobacter Spp

The aim of this study was to compare the phenotypic and genotypic based methods for species identification of thermotolerant campylobacters of human and food origin from the Czech Republic. Phenotypic methods are time-consuming and sometimes lead to intermediate results, therefore replacement by more specific and rapid methods are needed. Out of a total of 911 campylobacter strains tested, 800 human isolates were received from the clinical bacteriology laboratories from 5 regions and 111 foodstuff isolates (raw chicken and pork meat from retail market) originated from the routine examination in our laboratory. Based on the PCR method 85.1% of these strains were identified as C. jejuni, 12.5% as C. coli and 2.3% as mixed cultures of C. jejuni and C. coli. When species determination of campylobacters was based on conventional methods (hippurate hydrolysis test), 28.5% of the isolates were not identified correctly. The mixed cultures of campylobacters have not been detected without further subculturing of strains, which takes several days and enormously extends the identification process. The use of the PCR method showed to be a useful tool for species identification of Campylobacter spp.

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