scholarly journals Exon 9 LEPR Gene SNP Polymorphism of Hybrid Chickens F2Kambro Crossbreeds of ♀ F1Kambro with ♂ F1Kambro

2021 ◽  
Author(s):  
I Wayan Swarautama Mahardhika ◽  
Budi Setiadi Daryono
Keyword(s):  
Nucleotide Polymorphisms ◽  
Arms Pcr ◽  
Specific Protocol ◽  
Correlation Degree ◽  
Transversion Mutation ◽  
Gene Correlation ◽  
Exon 9 ◽  
Pcr Method ◽  
Dna Template

AbstractThe implementation of the T-ARMS PCR method in the detection of single nucleotide polymorphisms (SNPs) in the LEPR gene in chicken DNA samples has never been conducted. This research aims to design a specific protocol for exon 9 LEPR gene SNPs detection and detect LEPR gene expression or LEPR SNPs in Pelung chicken samples, F1Pelung, Layer, Broiler Cobb 500, F1Kambro chicken and F2Kambro chicken using the T-ARMS PCR method. Determination of LEPR gene correlation degree on Body Weight (BT) and Egg Productivity (PT) in F1 Kambro population and F2Kambro. Qualitative phenotype parameters showed six groups of segregated phenotypes compared to F1Kambro chicken. Growth of F2Kambro chicken weight reached 753.36 ± 155.31 grams in 8 weeks was not significant for F1Kambro chicken due to inbreeding depression (Fx = 25%, IR = 4.925%) and transversion of A LEPR allele mutations. Specific protocol detection of exon 9 LEPR gene SNPs using the T-ARMS PCR method can detect C127A LEPR mutations with IP: OP ratio 10:1 pmol / µM, chicken DNA template concentration of 100 ng / µL with annealing temperature of 55.7° C / 30s. The transversion mutation of C127A of LEPR exon 9 SNP were detected in DNA samples of F1Kambro hens (80%), F2Kambro roosters (20%), Broiler Cobb 500 hens (75%). The mutations were not detected in Layer, Pelung Blirik Hitam chicken and F1Pelung populations.

Determination of the alleles of HLA I class in Georgian, Kurdish and Czech populations by ARMS-PCR method

1996 ◽  
Vol 47 (1-2) ◽  
pp. 54
Author(s):  
Bendukidze Nina ◽  
Meschishvili Elena ◽  
Ivašková Eva ◽  
Černá Marie ◽  
Churadze Tamaz ◽  
...  
Keyword(s):  
Arms Pcr ◽  
Pcr Method

scholarly journals The Application of UTY and SRY Molecular Markers for Determination of Unknown Sex Samples in Bali Cattle

2020 ◽  
Vol 21 (1) ◽  
pp. 55
Author(s):  
Indriawati Indriawati ◽  
Slamet Diah Volkandari ◽  
Endang Tri Margawati
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Accurate Information ◽  
Blood Samples ◽  
Unknown Sample ◽  
Pooled Dna ◽  
Pcr Method ◽  
Bali Cattle ◽  
Dna Template

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.

scholarly journals Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits

2015 ◽  
Vol 58 (1) ◽  
pp. 165-169
Author(s):  
D. Liu ◽  
Z. Wang ◽  
W. Ma ◽  
Y. Gao ◽  
A. Li ◽  
...  
Keyword(s):  
Growth Traits ◽  
Average Daily Gain ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Interacting Protein ◽  
Association Analyses ◽  
Arms Pcr ◽  
Pcr Rflp ◽  
Cheap Method ◽  
Pcr Method

Abstract. Nuclear receptor-interacting protein 1 (NRIP1) specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors to inhibit transcription. Previous work has demonstrated this protein to be a key regulator in modulating transcriptional activity of many transcription factors, some of which are closely related to development and growth. In this study, we have successfully genotyped two newly identified bovine NRIP1 single-nucleotide polymorphisms (SNPs) (c.605A > G and c.1301G > A) using the T-ARMS-PCR method and validated the accuracy by means of PCR-RFLP assay using 1809 individuals of 9 different cattle breeds. The association analyses results indicated that c.605A > G locus was significantly associated with body weight and average daily gain in Nanyang cattle at 18 months (P < 0.05). Thus it can be inferred that T-ARMS-PCR is a rapid, reliable, and cheap method for SNP genotyping and that c.605A > G polymorphism in bovine NRIP1 is associated with growth traits. These findings will be of benefit for the application of DNA markers related to growth traits in marker-assisted selection (MAS), and will improve the promotion of beef cattle.

Development of a simple method for the determination of single nucleotide polymorphisms

2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Simple Method ◽  
Single Nucleotide

scholarly journals A rapid and cost‐effective multiplex ARMS‐PCR method for the simultaneous genotyping of the circulating SARS‐CoV‐2 phylogenetic clades

2021 ◽  
Author(s):  
Mohammad Tanvir Islam ◽  
ASM Rubayet Ul Alam ◽  
Najmuj Sakib ◽  
Mohammad Shazid Hasan ◽  
Tanay Chakrovarty ◽  
...  
Keyword(s):  
Cost Effective ◽  
Arms Pcr ◽  
Pcr Method

Determination of the correlation relationship between irrigation and distribution of agricultural lands

2021 ◽  
Vol 07 (02) ◽  
pp. 29-35
Author(s):  
Kamil Zakir oğlu İbrahimli ◽  
Keyword(s):  
Key Words ◽  
Statistical Method ◽  
Negative Correlation ◽  
Agricultural Lands ◽  
Correlation Relationship ◽  
Positive Correlation ◽  
Correlation Methods ◽  
Correlation Degree ◽  
The Relationship

The importance of the statistical method is great in the study of natural and economic processes. It is possible to conduct analyzes and obtain results with the help of this method. Sometimes phenomena and processes co-exist and develop interdependently. In this case, it is necessary to determine the relationship between them, to express this relationship. Key words: perfect positive correlation, perfect negative correlation, direction of correlation, degree of correlation, methods of calculating correlation, Spearman᾽s method

scholarly journals Identification of the Trichinella species by PCR method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 41-47
Author(s):  
Sasa Vasilev ◽  
Jelena Cvetkovic ◽  
Ivana Radovic ◽  
Ljiljana Sofronic-Milosavljevic
Keyword(s):  
Human Health ◽  
Health Problem ◽  
Continuous Monitoring ◽  
Human Consumption ◽  
Sylvatic Cycle ◽  
Trichinella Infection ◽  
High Prevalence ◽  
Pcr Method ◽  
Pig Meat

Serbia is country with a high prevalence of Trichinella infection in pigs, which continues to be a serious human health problem. In Serbia, only a few isolates of Trichinella found in pork have been genetically specified to date, and all were proven as T. spiralis. New data shows that in the sylvatic cycle in Serbia, at least in the Belgrade district, more than one Trichinella species co-exist (T. spiralis and T. britovi). Increased awareness of the possible overlap among sylvatic and domestic Trichinella cycles indicates the need for continuous monitoring of Trichinella species circulation and strongly points to the need that all isolates of Trichinella found in meat for human consumption should be subject to a determination of the Trichinella species (due to the risk of transmission of infection with T. britovi to domestic pigs and humans). This is why we examined using PCR the Trichinella larvae found in pig meat that caused a human outbreak (Trichinella infection) in Grocka (Belgrade district) during February 2011. The isolated larvae belonged to T. spiralis.

scholarly journals Histochemical analysis of skeletal muscular tissues of pigs according to genotype MYF4

2008 ◽  
Vol 24 (1-2) ◽  
pp. 117-126
Author(s):  
Vladimir Zimmermann ◽  
V. Kulísek ◽  
A. Copík ◽  
M. Odstrcil ◽  
Ondrej Debrecéni ◽  
...  
Keyword(s):  
Rectus Femoris ◽  
Muscle Fibres ◽  
Fat Cells ◽  
Average Thickness ◽  
Triceps Brachii ◽  
Surface Representation ◽  
Histochemical Analysis ◽  
Pcr Method ◽  
Muscular Tissues

The results of histochemical analysis of three muscles m. triceps brachii (MTB), m. longissimus thoracicus (MLT) and m. rectus femoris (MRF) of two groups of pigs created according to the genotypes MYF 4 are presented. Determination of MYF 4 genotypes was made by PCR method and for histochemical analysis was used 5 animals detected as homozygote MYF 4- AA type and 5 animals of heterozygote genotype myogenin-AB out of the total of 25 individual animals tested. The histochemical analysis proved that homozygotes AA have had bigger fat cells than heterozygotes AB in three studied muscles in average. The size of fat cells in MLT - 41.10?m or 38.50 ?m respectively dominated in both groups of animals. Percentage surface representation of interstitial tissues was higher in the studied muscles of heterozygote MYF 4-AB. The volume of ligaments was the highest in MRF (3.80% or 3.90% respectively) in both groups (myogenin - AA and AB). The average thickness was of three studied muscles muscle fibres higher at homozygote genotype myogenin-AA than in heterozygote myogenin-AB. The thickest fibres in both genotypes were in MRF (88.60 ?m, and 84.72 ?m respectively) and the lowest in MTB (73.30 and 69.40 ?m respectively). The highest values of muscle fibres thickness were detected in ?-White fibres. Their percentage surface representation corresponded to this in all three types of muscles of both studied genotype myogenin groups.

scholarly journals A Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e62126 ◽  
Author(s):  
Chen Zhang ◽  
Ying Liu ◽  
Brian Z. Ring ◽  
Kai Nie ◽  
Mengjie Yang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Arms Pcr
Sign in / Sign up

Export Citation Format

Share Document