Inhibition of growth and respiration of leishmania mexicana by the antitumor agent lonidamine

Inhibition of one Leishmania subspecies by exometabolites of another subspecies, a phenomenon not previously reported, is suggested by our recent observations in cell cloning experiments with Leishmania mexicana mexicana and Leishmania mexicana amazonensis. Clones were identified using the technique of schizodeme analysis. The phenomenon observed is clearly relevant to studies of parasite isolation, leishmanial metabolism, cross-immunity and chemotherapy.

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Gastric Ulcers Produced by Cisplatin

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099337 ◽

1981 ◽

Vol 39 ◽

pp. 582-583

Author(s):

S.K. Aggarwal ◽

J. San Antonio

Keyword(s):

Electron Microscopy ◽

Single Dose ◽

Side Effects ◽

Antitumor Agent ◽

Gastric Ulcers ◽

Enzyme Cytochemistry ◽

Intestinal Toxicity ◽

Ovarian Cancers ◽

Inner Surface

Cisplatin (cis-dichlorodiammineplatinum(II)) a potent antitumor agent is now available for the treatment of testicular and ovarian cancers. It is however, not free from its serious side effects including nephrotoxicity, gastro intestinal toxicity, myelosuppression, and ototoxicity. Here we now report that the drug produces peculiar bloating of the stomach in rats and induces acute ulceration.Wistar-derived rats weighing 200-250 g were administered cisplatin(9 mg/kg) ip as a single dose in 0.15 M NaCl. After 3 days the animals were sacrificed by decapitation. The stomachs were removed, the contents analyzed for pepsin and acidity. The inner surface was examined with a dissecting microscope after a moderate stretching for ulcers. Affected areas were fixed and processed for routine electron microscopy and enzyme cytochemistry.The drug treated animals kept on food and water consistently showed bloating and lesions (Fig. 1) with a frequency of 6-70 ulcers in the rumen section of the stomachs.

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Parathyroid gland before and after cisplatin treatment

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100128584 ◽

1987 ◽

Vol 45 ◽

pp. 860-861

Author(s):

S.K. Aggarwal ◽

J.M. Fadool

Keyword(s):

Parathyroid Gland ◽

Wistar Rats ◽

Antitumor Agent ◽

The Body ◽

Cross Linking ◽

Limiting Factor ◽

Hormonal Changes ◽

Primary Mechanism ◽

Before And After ◽

Dna Strands

Cisplatin (CDDP) a potent antitumor agent suffers from severe toxic side effects with nephrotoxicity being the major dose-limiting factor, The primary mechanism of its action has been proposed to be through its cross-linking DNA strands. It has also been shown to inactivate various transport enzymes and induce hypocalcemia and hypomagnesemia that may be the underlying cause for some of its toxicities. The present is an effort to study its influence on the parathyroid gland for any hormonal changes that control calcium levels in the body.Male Swiss Wistar rats (Crl: (WI) BR) weighing 200-300 g and of 60 days in age were injected (ip) with cisplatin (7mg/kg in normal saline). The controls received saline injections only. The animals were injected (iv) with calcium (0.5 ml of 10% calcium gluconate/day) and were killed by decapitation on day 1 through 5. Trunk blood was collected in heparinized tubes.

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Cytological changes induced by platinum-thymine in sarcoma-180 cells: Electron microscopy study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015900x ◽

1990 ◽

Vol 48 (3) ◽

pp. 290-291

Author(s):

Gustav Ofosu

Keyword(s):

Mammalian Cells ◽

Antitumor Agent ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Limiting Factor ◽

Sarcoma 180 ◽

Electron Microscopic ◽

Cytological Changes

Platinum-thymine has been found to be a potent antitumor agent, which is quite soluble in water, and lack nephrotoxicity as the dose-limiting factor. The drug has been shown to interact with DNA and inhibits DNA, RNA and protein synthesis in mammalian cells in vitro. This investigation was undertaken to elucidate the cytotoxic effects of piatinum-thymine on sarcoma-180 cells in vitro ultrastructurally, Sarcoma-180 tumor bearing mice were treated with intraperitoneal injection of platinum-thymine 40mg/kg. A concentration of 60μg/ml dose of platinum-thymine was used in in vitro experiments. Treatments were at varying time intervals of 3, 7 and 21 days for in vivo experiments, and 30, 60 and 120 min., 6, 12, and 24th in vitro. Controls were not treated with platinum-thymine.Electron microscopic analyses of the treated cells in vivo and in vitro showed drastic cytotoxic effect.

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Alterations of cell surfaces as a pathogenetic factor in psoriasis. Possible loss of contact inhibition of growth

Archives of Dermatology ◽

10.1001/archderm.107.1.38 ◽

1973 ◽

Vol 107 (1) ◽

pp. 38-46 ◽

Cited By ~ 30

Author(s):

C. E. Orfanos

Keyword(s):

Contact Inhibition ◽

Cell Surfaces ◽

Inhibition Of Growth ◽

Pathogenetic Factor ◽

Loss Of Contact

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DIFFERENCES IN GROWTH AND THYROID FUNCTION OF PEKIN AND MUSCOVY DUCKS EITHER UNTREATED OR TREATED WITH STILBOESTROL

Acta Endocrinologica ◽

10.1530/acta.0.0500411 ◽

1965 ◽

Vol 50 (3) ◽

pp. 411-417 ◽

Cited By ~ 3

Author(s):

Hans von Faber ◽

Wolfgang Häussermann

Keyword(s):

Thyroid Function ◽

Pekin Ducks ◽

Inhibition Of Growth ◽

Marked Inhibition ◽

Muscovy Ducks ◽

Effect On Growth

ABSTRACT Pekin ducks grow faster and show a faster and higher thyroidal 131I uptake than Muscovy ducks. Treatment with a total of 15 mg stilboestrol during the first 4 weeks had no effect on growth, thyroid weight or thyroidal 131I uptake in male or female Pekin ducklings. In male Muscovy ducklings the same treatment caused a marked inhibition of growth, a decrease in thyroid weight and a lowered thyroidal 131I uptake. Thyroxine, administered simultaneously in physiological doses, did not prevent inhibition of growth. It is therefore concluded that this inhibition is not due to the lowered thyroid function.

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ROLE OF THE THYROID GLAND IN DEVELOPMENT AND MAINTENANCE OF RENAL HYPERTENSION IN RATS

Acta Endocrinologica ◽

10.1530/acta.0.xxxiv0411 ◽

1960 ◽

Vol XXXIV (III) ◽

pp. 411-429 ◽

Cited By ~ 3

Author(s):

Melvin J. Fregly ◽

Kenneth M. Cook

Keyword(s):

Blood Pressure ◽

Rate Increase ◽

Renal Hypertension ◽

The Other ◽

Elevated Blood Pressure ◽

Unit Weight ◽

Elevated Blood ◽

Inhibition Of Growth ◽

Testicular Size

ABSTRACT The anti-thyroid drugs, thiouracil, propylthiouracil, and methimazole, prevented both development of elevated blood pressure and cardiac hypertrophy usually accompanying kidney encapsulation with latex envelopes. These drugs also reduced elevated blood pressure of rats with hypertension of 13 to 40 weeks' duration prior to drug administration. Addition of desiccated thyroid powder to diet containing an anti-thyroid drug overcame the anti-hypertensive effect of the latter. Withdrawal of thyroid powder only was followed by return of blood pressure to previous low level within 3 weeks. The results suggest that the anti-hypertensive effect of these drugs is related directly to the hypothyroidism produced rather than to extrathyroidal effects of the drugs. Comparison of potencies of the 3 drugs in terms of anti-hypertensive effect, inhibition of growth rate, increase in testicular size, and increase in thyroid size suggests that propylthiouracil and methimazole are equally potent per unit weight of drug. Thiouracil has approximately half the potency of the other two.

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Effect of Vial shaking on Loading Time of Epirubicin into Drug-Eluting Bead

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v5i3.8878 ◽

2015 ◽

Vol 5 (3) ◽

Cited By ~ 1

Author(s):

Kenji Ikeda ◽

Yusuke Kawamura ◽

Masahiro Kobayashi ◽

Taito Fukushima ◽

Yushi Sorin ◽

...

Keyword(s):

Antitumor Agent ◽

Loading Rates ◽

Vortex Mixer ◽

Long Time ◽

Group A ◽

Drug Eluting ◽

Large Hepatocellular Carcinoma ◽

Group B ◽

Time Required ◽

Short Time

Background: Although DC Bead has been useful in treatment of multiple and large hepatocellular carcinoma, loading time of doxorubicin into the DC Bead takes a long time of 30-120 minutes. Epirubicin is also used as an antitumor agent together with DC Bead, but its loading efficiency was not sufficiently elucidated. Methods: To shorten loading time of epirubicin into DC Bead (100-300µm, 300-500µm, 500-700µm), we examined the following three methods after mixing the drug: (a) let stand in room temperature, (b) agitated for 30 seconds with Vortex mixer, and (c) sonicated for 30 seconds with ultrasonic cleaner. After loading of epirubicin by each method, supernatant concentration for epirubicin was assayed at 5, 10, 30, 60, and 120 minutes. Results: Epirubicin loading rates for small bead (100-300µm) at 5 minutes were 82.9 % in group a, 93.8% in group b, and 79.9 % in group c. Similarly, medium bead (300-500µm), 40.1% in group a, 65.7% in group b and 45.5% in group c, respectively. In large-sized bead (500-700µm), loaded rates of epirubicin were 38.8% in group a, 59.0% in group b and 48.0% in group c. Agitation of mixture of epirubicin and DC Bead with Vortex mixer significantly shortened the loading time, but sonication did not affect the time required. Microscopic examination did not lead to any morphological change of microspheres in all the methods. Conclusions: Short time of agitation with Vortex mixer reduced the necessary time for loading of epirubicin in every standard of DC Bead.

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