In vitro immunomodulatory effects of traditional Kampo medicine (Sho-saiko-to: SST) on peripheral mononuclear cells in patients with AIDS

1990 ◽  
Vol 44 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Y Inada ◽  
K Watanabe ◽  
M Kamiyama ◽  
T Kanemitsu ◽  
WS Clark ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Kampo Medicine ◽  
Immunomodulatory Effects ◽  
Peripheral Mononuclear Cells

scholarly journals Increased susceptibility of peripheral mononuclear cells of leukemic patients to HTLV-I infection in vitro

Blood ◽  
1987 ◽  
Vol 69 (4) ◽  
pp. 1175-1181 ◽  
Author(s):  
G Graziani ◽  
D Pasqualetti ◽  
M Lopez ◽  
C D'Onofrio ◽  
AM Testi ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Healthy Controls ◽  
Nk Activity ◽  
Acute Leukemias ◽  
Normal Cells ◽  
Healthy Donors ◽  
Peripheral Mononuclear Cells ◽  
Cord Blood Lymphocytes ◽  
Increased Susceptibility

Abstract Peripheral mononuclear cells (MNC) collected from 12 healthy donors and 44 leukemic patients at various stages of the disease were tested for natural killer (NK) activity and for their susceptibility to HTLV-I infection in vitro, measured in terms of percentage of p19 positive cells. MNC from leukemic donors at any stage of leukemia (ie, onset or relapse, ON/REL; complete remission or off-therapy, CR/OT donors) were highly susceptible to HTLV-I infection. This was true for acute leukemias of lymphoblastic (ALL) or nonlymphoblastic (ANLL) type. MNC of ON/REL patients were more susceptible to HTLV-I than those of CR/OT donors. In addition, leukemic blasts were more rapidly infected (ie, within five to seven days) than the HTLV-I-susceptible normal cord- blood lymphocytes. However, the presence of circulating blasts was not essential to virus susceptibility, since CR/OT MNC, presumably free of leukemic blasts, were still more susceptible to HTLV-I than normal cells. Basal NK function of MNC from leukemic patients was significantly lower than that detectable in healthy controls. However, no correlation was found between susceptibility to HTLV-I infection and NK activity.

Searching for Specific Responses to Copper Exposure: An In Vitro Copper Challenge in Peripheral Mononuclear Cells

2010 ◽  
Vol 142 (3) ◽  
pp. 407-414
Author(s):  
Miguel Arredondo ◽  
Alejandra Espinoza ◽  
Fernando Pizarro ◽  
Magdalena Araya
Keyword(s):  
Mononuclear Cells ◽  
Copper Exposure ◽  
Peripheral Mononuclear Cells

scholarly journals The aminobisphosphonate pamidronate controls influenza pathogenesis by expanding a γδ T cell population in humanized mice

2011 ◽  
Vol 208 (7) ◽  
pp. 1511-1522 ◽  
Author(s):  
Wenwei Tu ◽  
Jian Zheng ◽  
Yinping Liu ◽  
Sin Fun Sia ◽  
Ming Liu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
T Cell ◽  
Viral Replication ◽  
Mononuclear Cells ◽  
Immunodeficient Mice ◽  
H5n1 Influenza ◽  
Infected Cells ◽  
Peripheral Mononuclear Cells ◽  
Vγ9vδ2 T Cells

There are few antiviral drugs for treating influenza, and the emergence of antiviral resistance has further limited the available therapeutic options. Furthermore, antivirals are not invariably effective in severe influenza, such as that caused by H5N1 viruses. Thus, there is an urgent need to develop alternative therapeutic strategies. Here, we show that human Vγ9Vδ2 T cells expanded by the aminobisphosphonate pamidronate (PAM) kill influenza virus–infected cells and inhibit viral replication in vitro. In Rag2−/−γc−/− immunodeficient mice reconstituted with human peripheral mononuclear cells (huPBMCs), PAM reduces disease severity and mortality caused by human seasonal H1N1 and avian H5N1 influenza virus, and controls the lung inflammation and viral replication. PAM has no such effects in influenza virus–infected Rag2−/−γc−/− mice reconstituted with Vγ9Vδ2 T cell–depleted huPBMCs. Our study provides proof-of-concept of a novel therapeutic strategy for treating influenza by targeting the host rather than the virus, thereby reducing the opportunity for the emergence of drug-resistant viruses. As PAM has been commonly used to treat osteoporosis and Paget’s disease, this new application of an old drug potentially offers a safe and readily available option for treating influenza.

scholarly journals Increased susceptibility of peripheral mononuclear cells of leukemic patients to HTLV-I infection in vitro

Blood ◽  
1987 ◽  
Vol 69 (4) ◽  
pp. 1175-1181
Author(s):  
G Graziani ◽  
D Pasqualetti ◽  
M Lopez ◽  
C D'Onofrio ◽  
AM Testi ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Healthy Controls ◽  
Nk Activity ◽  
Acute Leukemias ◽  
Normal Cells ◽  
Healthy Donors ◽  
Peripheral Mononuclear Cells ◽  
Cord Blood Lymphocytes ◽  
Increased Susceptibility

Peripheral mononuclear cells (MNC) collected from 12 healthy donors and 44 leukemic patients at various stages of the disease were tested for natural killer (NK) activity and for their susceptibility to HTLV-I infection in vitro, measured in terms of percentage of p19 positive cells. MNC from leukemic donors at any stage of leukemia (ie, onset or relapse, ON/REL; complete remission or off-therapy, CR/OT donors) were highly susceptible to HTLV-I infection. This was true for acute leukemias of lymphoblastic (ALL) or nonlymphoblastic (ANLL) type. MNC of ON/REL patients were more susceptible to HTLV-I than those of CR/OT donors. In addition, leukemic blasts were more rapidly infected (ie, within five to seven days) than the HTLV-I-susceptible normal cord- blood lymphocytes. However, the presence of circulating blasts was not essential to virus susceptibility, since CR/OT MNC, presumably free of leukemic blasts, were still more susceptible to HTLV-I than normal cells. Basal NK function of MNC from leukemic patients was significantly lower than that detectable in healthy controls. However, no correlation was found between susceptibility to HTLV-I infection and NK activity.

scholarly journals Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-20
Author(s):  
Takahiro Teshima ◽  
Yunosuke Yuchi ◽  
Ryohei Suzuki ◽  
Hirotaka Matsumoto ◽  
Hidekazu Koyama
Keyword(s):  
T Cells ◽  
Extracellular Vesicles ◽  
Cd8 T Cells ◽  
Immune Cells ◽  
Cd4 T Cells ◽  
Mononuclear Cells ◽  
Immunomodulatory Effects ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Adipose tissue-derived mesenchymal stem cells (ADSCs) have anti-inflammatory and immunomodulatory characteristics. Many studies have suggested that the immunomodulation of ADSCs is largely mediated by secreted paracrine factors. Various factors are secreted from ADSCs, among which extracellular vesicles are considered to play a major role in the communication between ADSCs and target cells. Several studies have reported the function of canine ADSC-derived extracellular vesicles (cADSC-EVs), but few studies have reported the immunomodulatory effects of cADSC-EVs on immune cells. The purpose of this study was to investigate the effects of cADSC-EVs on in vitro-stimulated CD4+ T cells isolated from peripheral blood mononuclear cells (PBMCs). cADSC-EVs were isolated from cADSCs under naive conditions or primed conditions by tumor necrosis factor-α (TNFα) and interferon-γ (IFNγ). The expression levels of several microRNAs in cADSC-EVs were altered by priming with TNFα and IFNγ. Culturing PBMCs stimulated with concanavalin A in the presence of naive or primed cADSC-EVs inhibited the differentiation of PBMCs and CD4+ T cells and promoted apoptosis of PBMCs. CD4+, CD8+, and CD4+CD8+ T cells were decreased, while CD3+CD4-CD8- T cells were increased. T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cells were analyzed by flow cytometry. cADSC-EVs inhibited the proliferation of Th1 and Th17 cells and enhanced Th2 and Treg cell proliferation. However, CD4+ T cells that had incorporated labeled cADSC-EVs comprised only a few percent of all cells. Therefore, these responses of stimulated CD4+ T cells may be due to not only direct effects of cADSC-EVs but also to indirect effects through interactions between cADSC-EVs and other immune cells. In conclusion, cADSC-EVs exert immunosuppressive effects on stimulated CD4+ T cells in vitro. These findings may be useful for further studies of immune diseases.

Sign in / Sign up

Export Citation Format

Share Document