GC determination of dimethyl selenide and trimethyl selenonium ions in aquatic systems using element specific detection

1990 ◽  
Vol 24 (12) ◽  
pp. 3099-3102 ◽  
Author(s):  
Dieter Tanzer ◽  
Klaus G. Heumann
2000 ◽  
Vol 18 (No. 3) ◽  
pp. 103-109 ◽  
Author(s):  
K. Zdeňková ◽  
K. Demnerová ◽  
G. Jeníková ◽  
J. Pazlarová

Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which represents its main means of entry into the organism, is a topic of special importance. The original classic methods for the determination of Listeria monocytogenes are in general laborious and time-consuming procedures. In order to address this issue we developed a new rapid method for specific detection of Listeria monocytogenes in food samples. The method consists of three steps: i) enrichment of food microflora (16 h), ii) selective isolation of Listeria sp. exploiting immunomagnetic separation (2–3 h) followed by iii) precise identification of Listeria sp. and Listeria monocytogenes using duplex PCR. PCR primers specific to part of 16S rRNA were used in order to identify the members of Listeria genus. The specific identification of Listeria monocytogenes was accomplished exploiting a pair of primers specific to gene encoding invasion-associated protein – iap (4–5 h). Amplification products, 1003 bp and 593 bp respectively, were separated by electrophoresis and visualized by UV detection. The optimized IMS-PCR method was used to test the presence of Listeria sp. and Listeria monocytogenes in food samples (ground meat, low-fat milk and cheese [olomoucké tvarůžky]). A comparison of the efficiency of the bacteria enrichment step by IMS and centrifugation was also performed. The analysis time including enrichment is less than 24 h. The detection limit for Listeria monocytogenes was found between 101–102 cfu per 25 g of food sample.


2016 ◽  
Vol 92 (01) ◽  
pp. 62-65 ◽  
Author(s):  
Richard Petrone ◽  
Kevin J. Devito ◽  
Carl Mendoza

The Hydrology Ecology And Disturbance (HEAD-1 and HEAD-2) research programs in the Western Boreal Plains of North-Central Alberta, has provided objective delineation and determination of landscape units characterized by geology and climate. From these landscape indices can be developed that provide information on the scale at which forest, wetland, and aquatic systems are linked to their surroundings and the potential response of an area to particular disturbances. In collaboration with industry, government and NGO planners and ecologists this work establishes a hydrologic risk planning process that evaluates the ecological risk and monetary costs of forest harvest on forest succession and water quality and quantity.


2017 ◽  
Vol 50 (4) ◽  
pp. 2137 ◽  
Author(s):  
K. Giouri ◽  
M. Vavelidis ◽  
V. Melfos ◽  
L. Papadopoulou

In surface aquatic systems, trace elements and especially heavy metals accumulate in sediments. Thus, the determination of sediment chemical characteristics is critical in the assessment of an aquatic environment’s quality. Thirteen sediment samples from Palea Kavala river (NE Macedonia, Greece) were studied for their content in Al, Fe, Cu, Cd, Mn, Pb, Zn. In order to evaluate the metal enrichment for environmental purposes, two geochemical indices were employed: Enrichment Factor (EF) and Geoaccumulation Index (Igeo). According to the results, Al was the most abundant major element in sediments with Fe and Mn to follow. Trace element content followed the order Pb>Zn>Cu>Cd. EF values revealed that samples are enriched in Cd and Pb, while Igeo values also suggested that there is considerable contamination concerning Cd and Pb. It is concluded that ore mineralizations which occur in Palea Kavala region contribute to the elevated concentrations of all the studied elements, since they comprise mainly Fe-Mn metal assemblages including minerals such as pyrite, goethite, chalcopyrite, galena, sphalerite and pyrolousite.


Sign in / Sign up

Export Citation Format

Share Document