Effects of Bivalent Cations and Proteins on Thermotropic Properties of Phospholipid Membranes

1977 ◽  
pp. 485-496
Author(s):  
DEMETRIOS PAPAHADJOPOULOS
Keyword(s):  
Phospholipid Membranes ◽  
Bivalent Cations ◽  
Thermotropic Properties

Electronmicroscopic localization of ribonucleic acids in ciliate Bursaria truncatella during cell division

1990 ◽  
Vol 48 (3) ◽  
pp. 132-133
Author(s):  
Vladimir Popenko ◽  
Natalya Cherny ◽  
Maria Yakovleva
Keyword(s):  
Cell Division ◽  
High Specificity ◽  
Longitudinal Axis ◽  
Gold Complexes ◽  
Somatic Nucleus ◽  
Ribonucleic Acids ◽  
Biological Meaning ◽  
Bivalent Cations ◽  
Lowicryl K4m ◽  
Short Time

Highly polyploid somatic nucleus (macronucleus) of ciliate Bursaria truncatella under goes severe changes in morphology during cell division. At first, macronucleus (Ma) condences, diminishes in size and turns perpendicular to longitudinal axis of the cell. After short time, Ma turns again, elongates and only afterwards the process of division itself occurs. The biological meaning of these phenomena is not clear.Localization of RNA in the cells was performed on sections of ciliates B. truncatella, embedded in “Lowicryl K4M” at various stages: (1) before cell division (Figs. 2,3); (11) at the stage of macronucleus condensation; (111) during elongation of Ma (Fig.4); (1111) in young cells (0-5min. after division). For cytochemical labelling we used RNaseAcolloidal gold complexes (RNase-Au), which are known to bind to RNA containing cell ularstructures with high specificity. The influence of different parameters on the reliability and reproducibility of labelling was studied. In addition to the factors, discussed elsewhere, we found that the balance of mono- and bivalent cations is of great significance.

Interaction of Vasopressin with Human Blood Platelets: Dependency on Mg2+

1986 ◽  
Vol 56 (03) ◽  
pp. 260-262 ◽  
Author(s):  
Isabella Roos ◽  
Fabrizia Ferracin ◽  
Alfred Pletscher
Keyword(s):  
Phosphatidic Acid ◽  
Human Blood ◽  
Arginine Vasopressin ◽  
Specific Binding ◽  
Blood Platelets ◽  
Bivalent Cations ◽  
Platelet Membranes ◽  
Maximal Rise ◽  
Human Blood Platelets ◽  
Stimulation Of

SummaryArginine-vasopressin (AVP) in the presence of Mg2+ but not in the absence of bivalent cations led to accumulation of [32P]-phosphatidic acid ([32P]-PA) in human blood platelets. Mg2+ also enhanced the specific binding of [3H]-AVP to intact platelets. The concentrations of the cation which enabled AVP to cause half maximal rise of [32P]-PA and those inducing half maximal [3H]-AVP-binding were of the same order. It is concluded that the stimulation of phosphatidyl inositide breakdown by AVP in presence of Mg2+ is at least partially due to a Mg2+-induced enhancement of specific AVP-binding to the platelet membranes.

Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition

2020 ◽  
Vol 21 (13) ◽  
pp. 1304-1315
Author(s):  
Junmei Zhou ◽  
Lianghong Yin ◽  
Chenbin Wu ◽  
Sijia Wu ◽  
Jidong Lu ◽  
...  
Keyword(s):  
Bacterial Isolate ◽  
Ammonium Oxalate ◽  
Fermentation Condition ◽  
Bivalent Cations ◽  
Rdna Sequencing ◽  
Optimum Ph ◽  
Alkaline Conditions ◽  
Fermentation Parameters ◽  
Pulp And Paper Industries ◽  
Central Composite

Objective: Alkaline Carboxymethyl Cellulase (CMCase) is an attractive enzyme for the textile, laundry, pulp, and paper industries; however, commercial preparations with sufficient activity at alkaline conditions are scarce. Methods: High CMCase-producing bacterial isolate, SX9-4, was screened out from soil bacteria, which was identified as Flavobacterium sp. on the basis of 16S rDNA sequencing. Results: The optimum pH and temperature for CMCase reaction were 8.0 and 55°C, respectively. Alkaline CMCase was stable over wide pH (3.0-10.6) and temperature (25-55°C) ranges. Enzyme activity was significantly inhibited by the bivalent cations Mn2+ and Cu2+, and was activated by Fe2+. To improve the alkaline CMCase production of SX9-4, fermentation parameters were selected through onefactor- at-a-time and further carried out by response surface methodologies based on a central composite design. Conclusion: High CMCase production (57.18 U/mL) was achieved under the optimal conditions: 10.53 g/L carboxymethylcellulose sodium, 7.74 g/L glucose, 13.71 g/L peptone, and 5.27 g/L ammonium oxalate.

Association of the polioviral RNA polymerase complex with phospholipid membranes.

1976 ◽  
Vol 19 (2) ◽  
pp. 457-466 ◽  
Author(s):  
B E Butterworth ◽  
E J Shimshick ◽  
F H Yin
Keyword(s):  
Rna Polymerase ◽  
Phospholipid Membranes ◽  
Polymerase Complex

A family of uranyl oxide hydrate phases with bivalent cations [MII(H2O)4][(UO2)3O3(OH)2]·H2O (MII – Mn, Co, Ni, Zn): synthesis, characterization and chemical stability in aqueous solutions

2021 ◽  
Author(s):  
Nikolay G. Chernorukov ◽  
Oxana V. Nipruk ◽  
Kseniya A. Klinshova ◽  
Olga N. Tumaeva ◽  
Dmitry V. Sokolov
Keyword(s):  
Aqueous Solutions ◽  
Nuclear Fuel ◽  
Chemical Stability ◽  
Fuel Cycle ◽  
Nuclear Fuel Cycle ◽  
Oxide Hydrate ◽  
Uranium Compounds ◽  
Bivalent Cations

A series of new uranium compounds [MII(H2O)3][(UO2)3O3(OH)2]·2H2O (MII – Mn, Co, Ni, Zn) were synthesized for binding radionuclides in the environment and nuclear fuel cycle.

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