Methods for Evaluation of Rat Epididymal Sperm Morphology

1993 ◽  
pp. 334-343 ◽  
Author(s):  
Ron Filler
Keyword(s):  
Sperm Morphology ◽  
Epididymal Sperm

scholarly journals Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus)

ISRN Anatomy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
A. M. Cruceño ◽  
J. C. de Rosas ◽  
M. Fóscolo ◽  
E. M. Chaves ◽  
L. Scardapane ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Reproductive Cycle ◽  
Sperm Morphology ◽  
Structural Parameters ◽  
Light And Electron Microscopy ◽  
Morphological Characteristics ◽  
Activity Period ◽  
Epididymal Sperm ◽  
Wild Rodent ◽  
Lagostomus Maximus

The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

scholarly journals The correlation between spermatozoa morphometry and the testosterone levels in the epididymis of aceh cow

2020 ◽  
Vol 151 ◽  
pp. 01020
Author(s):  
Sri Wahyuni ◽  
Gholib Gholib ◽  
Rizki Maulana ◽  
Dasrul Dasrul ◽  
Tongku N. Siregar ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Pearson Correlation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epididymal Sperm ◽  
Alternative Source ◽  
Testosterone Concentration ◽  
Pearson Correlation Test ◽  
Sperm Morphometry ◽  
Morphology And Morphometry ◽  
One Way Anova

The objective of this study was to determine the correlation between spermatozoa morphometry and testosterone concentration in the epididymal caput, corpus, and cauda of aceh cattle. The epididymal sperm swab slides were stained using Williams's staining to examined the morphology and morphometry of spermatozoa whereas the level of testosterone from epididymal caput, corpus, and cauda extract was measured using enzyme-linked immunosorbent assay (ELISA). The data of sperm morphology were presented descriptively whereas the data of sperm morphometry and testosterone concentration were analyzed using one way ANOVA. Pearson correlation test was conducted to know the correlation between the total length of spermatozoa (TLS) and testosterone concentration in three parts of epididymis. The result showed that the sperm morphology in caput, corpus, and cauda of epididymis was not different statistically. The TLS of aceh cattle at caput, corpus, and cauda epididymis were 69,70±0,8 µm, 70,90±0.39 µm, and 72,98±0.74 µm respectively and statistically different (p<0.05). In addition, the concentration of testosterone in caput, corpus, and cauda epididymis were 9,92±3,76 ng/g, 16.96±13,22 ng/g, and 15,63±11.80 ng/g respectively and also not different among three parts of epididymis (p>0.05). The positive correlation (r= 0.413, p= 0.416) was found between TLS and testosterone concentration at caput whereas in corpus and cauda those correlation were negative and not different (corpus: r= -0.638, p= 0.172 and cauda: r= -0.719, p= 0,108). In conclusion, the longest-sized spermatozoa found in the cauda epididymis are mature spermatozoa where the maturation process optimally takes place in caput epididymis. Testosterone found in the epididymis of aceh cow can be used as an alternative source of testosterone.

168 POST-THAW VIABILITY OF EPIDIDYMAL SPERM FROM WHITE-TAIL DEER THAT WAS FROZEN USING GLYCEROL OR DMSO AS THE CRYOPROTECTANT

2009 ◽  
Vol 21 (1) ◽  
pp. 183
Author(s):  
M. A. Stout ◽  
J. S. Saenz ◽  
G. T. Gentry ◽  
S. P. Leibo ◽  
K. R. Bondioli ◽  
...  
Keyword(s):  
Treatment Group ◽  
Sperm Morphology ◽  
Membrane Integrity ◽  
Slow Cool ◽  
Epididymal Sperm ◽  
Cold Environments ◽  
Normal Sperm ◽  
Nitrogen Vapor ◽  
Dmso Treatment ◽  
Medial Section

Cryoprotectants are compounds that protect and maintain the viability of cells when being subjected to cold environments. In addition to glycerol, DMSO has been evaluated for post-thaw epididymal sperm viability in various endangered species, but has not yet been assessed in White-tail deer. Testicles (in the scrotum) were collected from hunter harvested sexually mature White-tail bucks (n = 7) during the peak rutting season. Testicles within the scrotum, once removed from the postmortem buck, were placed in a plastic Ziploc bag and then into a Styrofoam ice chest (at ambient temperature) and transported to the Embryo Biotechnology Laboratory (EBL). Upon arrival at the EBL, sperm were flushed in a retrograde flow out of a small incision at the medial section of the cauda epididymides. Epididymal sperm were flushed from the epididymis using a non glycerol extender. Sperm from both epididymides of each buck were pooled and allowed a slow cool to 4°C. Upon reaching 4°C, the pooled epididymal sperm were equally divided and either 6% glycerol (A) or 6% DMSO (B) was added to each sperm sample. After the addition of the cryoprotectant sperm were loaded into 0.5-mL plastic straws and cryopreserved using liquid nitrogen vapor. Total motility (TM) was determined subjectively using an inverted Nikon Diaphot microscope. Membrane integrity (MI) was determined using SYBR 14 and propidium iodide staining under a microscope equipped with epiflurescence. Morphology was determined using an eosin-nigrosin staining. A paired t-test was used for statistical analyses. There were no differences between the glycerol and DMSO treatments for any of the three parameters measured (Table 1). However, post-thaw total motility and membrane integrity were significantly lower for both treatment groups (A and B) when compared with pre-freeze values. Furthermore, the DMSO treatment group had significantly lower normal morphology when compared with pre-freeze values, but was not different than the glycerol treatment group. Our results indicate that cryopreservation of epididymal sperm from White-tail deer with either 6% glycerol or 6% DMSO yield similar results for total motility, membrane integrity, and normal sperm morphology. Table 1.Pre-freeze and post-thaw total sperm motility, membrane integrity and normal sperm morphology for White-tail deer epididymal sperm Tony Vidrine of the Louisiana Department of Wildlife and Fisheries.

Testicular testosterone: Estradiol ratio in domestic cats and its relationship to spermatogenesis and epididymal sperm morphology

2012 ◽  
Vol 78 (6) ◽  
pp. 1224-1234 ◽  
Author(s):  
G. Müller ◽  
A.J. Martino-Andrade ◽  
A.S. Santos ◽  
A.L. Reghelin ◽  
D.M. Garcia ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Domestic Cats ◽  
Epididymal Sperm

scholarly journals The frequency of morphological defects of epididymal sperm in exotic animal species of the family Bovidae kept in the Czech Republic

2021 ◽  
Vol 90 (3) ◽  
pp. 263-270
Author(s):  
Jaroslav Šípek ◽  
Věra Kopecká ◽  
Petra Přinosilová ◽  
Jiří Rubeš
Keyword(s):  
Czech Republic ◽  
Animal Species ◽  
Sperm Morphology ◽  
Epididymal Sperm ◽  
The Czech Republic ◽  
Developmental Abnormalities ◽  
Domestic Species ◽  
The Family ◽  
Morphological Defects ◽  
Exotic Animal

Epididymal sperm morphology was evaluated by strict criteria using the DeSMA software. A total of 134 animals from the Dvůr Králové ZOO were included in the study. The aim of this study was to determine the prevalence of morphological defects of epididymal sperm in animals of the family Bovidae in species that are not native to the Czech Republic and are bred here only in zoos. Our results showed that the frequency of abnormalities was higher in exotic species of the Bovidae family compared to domestic species. Furthermore, the acquired defects prevailed over developmental abnormalities. Cytoplasmic droplets are a characteristic feature of epididymal sperm. Their average prevalence in the whole Bovidae family is almost 30%. However, distal droplets are not considered to be sperm pathology and, therefore, they are not the most frequent defect. The most common abnormalities are acquired head defects, followed by acquired tail defects, and then by acquired neck defects, mainly represented by proximal cytoplasmic droplets. The lowest prevalence was found for developmental abnormalities, with heads being more often affected than tails. The remaining defects were sporadic.

119: Effect of Epididymitis on Semen Biochemical and Sperm Morphology Parameters

2005 ◽  
Vol 173 (4S) ◽  
pp. 32-32
Author(s):  
Petra Huwe ◽  
Roelof Menkveld ◽  
Martin Ludwig ◽  
Wolfgang Weidner
Keyword(s):  
Sperm Morphology ◽  
Morphology Parameters
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