Development of Efficient Production System

ABSTRACTThe fungusThelonectria discophoraSANK 18292 produces the iminosugar nectrisine, which has a nitrogen-containing heterocyclic 5-membered ring and acts as a glycosidase inhibitor. In our previous study, an oxidase (designated NecC) that converts 4-amino-4-deoxyarabinitol to nectrisine was purified fromT. discophoracultures. However, the genes required for nectrisine biosynthesis remained unclear. In this study, the nectrisine biosynthetic gene cluster inT. discophorawas identified from the contiguous genome sequence around thenecCgene. Gene disruption and complementation studies and heterologous expression of the gene showed thatnecA,necB, andnecCcould be involved in nectrisine biosynthesis, during which amination, dephosphorylation, and oxidation occur. It was also demonstrated that nectrisine could be produced by recombinantEscherichia colicoexpressing thenecA,necB, andnecCgenes. These findings provide the foundation to develop a bacterial production system for nectrisine or its intermediates through genetic engineering.IMPORTANCEIminosugars might have great therapeutic potential for treatment of many diseases. However, information on the genes for their biosynthesis is limited. In this study, we report the identification of genes required for biosynthesis of the iminosugar nectrisine inThelonectria discophoraSANK 18292, which was verified by disruption, complementation, and heterologous expression of the genes involved. We also demonstrate heterologous production of nectrisine by recombinantE. coli, toward developing an efficient production system for nectrisine or its intermediates through genetic engineering.

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A modular environmental and economic assessment applied to the production of Hermetia illucens larvae as a protein source for food and feed

The International Journal of Life Cycle Assessment ◽

10.1007/s11367-021-01986-y ◽

2021 ◽

Author(s):

Raphaela Spykman ◽

Sayed Mahdi Hossaini ◽

Daniela A. Peguero ◽

Ashley Green ◽

Volker Heinz ◽

...

Keyword(s):

Environmental Impacts ◽

Production System ◽

Assessment System ◽

Microwave Drying ◽

Efficient Production ◽

Hermetia Illucens ◽

Processing Technologies ◽

Time Investment ◽

Efficiency Assessment ◽

Assessment Approach

Abstract Purpose The inclusion of insect protein into the food system has been proposed as a promising solution to ensure future food security and mitigate negative environmental impacts related to food production. However, the market volume for edible insects in Europe is still small; consequently, producers need a decision-support system to ensure the sustainable upscaling of the sector. The study analyzed environmental and economic impacts of insect production to identify the most eco-efficient production scenarios. Methods A novel modular eco-efficiency assessment approach was developed to analyze the production of dried Hermetia illucens larvae. An exemplary, industrial-scale insect production system was disaggregated into a total of 29 module variants that can be combined into 4608 distinct production scenarios, which are characterized by different feeds, energy efficiencies, and processing technologies. Environmental life cycle and cost assessments were carried out for each module variant, and eco-efficiency assessment was used to jointly assess these two sustainability dimensions. Additionally, the influence of the insect feed on the production system performance and impact was investigated by employing feed-specific scaling factors. These were used to aggregate module results into production scenario results. Results and discussion The most eco-efficient production scenarios include energy-efficient rearing facilities that rely on blanching and microwave drying for processing. The insect feed is the largest contributor to the environmental impacts and costs, but from an eco-efficiency standpoint, the choice of feed might not be crucial. Waste-type feeds (e.g., manure, fruit, and vegetable waste) have low environmental impacts and costs, but the production scenarios based on these feeds are less efficient. The low impacts of the feed are offset by higher impacts during the rearing and processing stages. Conversely, scenarios based on higher quality feeds (e.g., by-products like wheat middlings or distiller’s grains) require less resources, but the initial feed impacts and costs are higher. Moreover, of the feed types studied, only highly processed ones, such as compound chicken feed, should be avoided for insect rearing. Conclusions The developed modular assessment approach is efficient in assessing multiple potential insect production scenarios. It can be adapted to incorporate additional variations of the production system via additional modules. Limitations include the potential for redundant module combinations and the up-front time investment needed. Finally, the results are sensitive to methodological choices: thus, these should be carefully considered and communicated during the design of the modular assessment system.

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Implementasi Algoritma Wagner-Within pada Manajemen Inventori di PT X

Business and Finance Journal ◽

10.33086/bfj.v3i1.420 ◽

2018 ◽

Vol 3 (1) ◽

Cited By ~ 2

Author(s):

Puspandam Katias ◽

Achmad Affandi

Keyword(s):

Production System ◽

Manufacturing Industry ◽

Raw Materials ◽

Raw Material ◽

Efficient Production ◽

Manufacturing Companies ◽

Inventory Cost ◽

Qualitative Descriptive ◽

Total Inventory ◽

Supporting Material

The increasing competition in the manufacturing industry caused increasing inconsumer demand of the quality and quantity of a good product. Therefore, manufacturing companies must have reliable services, policies and product qualities to satisfy its customers. So it needs to be supported by efficient production system and inventory system. To able to create an efficient production system then need a good raw material inventory planning. This research aims to compere how efficiently in planning raw materials inventory between Wagner-Within Algorithm with the actual concept that applied to PT X, Sidoarjo. The methodology of this research is qualitative descriptive research. The findings this research is engaged in packaging (woven bag and jumbo bag) with main raw materials such as plastic ore and supporting material in inner, thread, additive, and pigment. Based on the result of the analysis is known that the actual concept of the company gives the total inventory cost IDR 3.151.000.000 with the frequency of ordering 12 times while WagnerWithin Algorithm method provides a more efficient total inventory cost of Rp. 2.685.821.101 with8 times the frequency of ordering and can savings of 14.8% of total raw material inventory cost.

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A Production Planning Based on Shipping Record for Food Company

2020 International Symposium on Flexible Automation ◽

10.1115/isfa2020-9627 ◽

2020 ◽

Author(s):

Hiroya Uematsu ◽

Tatsuhiko Sakaguchi ◽

Naoki Uchiyama ◽

Daichi Hiramatsu ◽

Kensuke Hiramatsu

Keyword(s):

Production Planning ◽

Production System ◽

Numerical Experiments ◽

Efficient Production ◽

Traditional Food ◽

Demand Prediction ◽

Material Requirement Planning ◽

Material Requirement ◽

Make To Stock ◽

Stock Information

Abstract The production system of the food factory is basically a make-to-stock production. Therefore, to achieve efficient production, the integration of demand, production, and stock information is essential. In this paper, we propose a production planning method based on the demand prediction in order to decrease the stock in the factory of Japanese traditional food ‘Tsukudani’. In the proposed method, the demand is first predicted according to the shipment records, then the material requirement planning and the capacity requirement planning are done based on the demand prediction. Finally, we carry out numerical experiments to validate the effectiveness of the proposed method.

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Synthesis of two new lipid mediators from docosahexaenoic acid by combinatorial catalysis involving enzymatic and chemical reaction

Scientific Reports ◽

10.1038/s41598-020-76005-5 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Jong-Jae Yi ◽

Sun-Yeon Heo ◽

Jung-Hyun Ju ◽

Baek-Rock Oh ◽

Woo Sung Son ◽

...

Keyword(s):

Docosahexaenoic Acid ◽

Chemical Reaction ◽

Production System ◽

Enzymatic Reaction ◽

Lipid Mediators ◽

Ligand Docking ◽

Efficient Production ◽

Omega 3 ◽

Chemical Catalysis ◽

New Type

Abstract Omega-3 polyunsaturated fatty acids (PUFAs) have been known to have beneficial effects in the prevention of various diseases. Recently, it was identified that the bioactivities of omega-3 are related to lipid mediators, called pro-resolving lipid mediators (SPMs), converted from PUFAs, so they have attracted much attention as potential pharmaceutical targets. Here, we aimed to build an efficient production system composed of enzymatic and chemical catalysis that converts docosahexaenoic acid (DHA) into lipid mediators. The cyanobacterial lipoxygenase, named Osc-LOX, was identified and characterized, and the binding poses of enzyme and substrates were predicted by ligand docking simulation. DHA was converted into three lipid mediators, a 17S-hydroxy-DHA, a 7S,17S-dihydroxy-DHA (RvD5), and a 7S,15R-dihydroxy-16S,17S-epoxy-DPA (new type), by an enzymatic reaction and deoxygenation. Also, two lipid mediators, 7S,15R,16S,17S-tetrahydroxy-DPA (new type) and 7S,16R,17S-trihydroxy-DHA (RvD2), were generated from 7S,15R-dihydroxy-16S,17S-epoxy-DPA by a chemical reaction. Our study suggests that discovering new enzymes that have not been functionally characterized would be a powerful strategy for producing various lipid mediators. Also, this combination catalysis approach including biological and chemical reactions could be an effective production system for the manufacturing lipid mediators.

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