Soybean ATP sulfurylase, a homodimeric enzyme involved in sulfur assimilation, is abundantly expressed in roots and induced by cold treatment

2006 ◽  
Vol 450 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Pallavi Phartiyal ◽  
Won-Seok Kim ◽  
Rebecca E. Cahoon ◽  
Joseph M. Jez ◽  
Hari B. Krishnan
Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1681-1686 ◽  
Author(s):  
Rachel Pinto ◽  
Quing Xui Tang ◽  
Warwick J. Britton ◽  
Thomas S. Leyh ◽  
James A. Triccas

Sulfur metabolism has been implicated in the virulence, antibiotic resistance and anti-oxidant defence of Mycobacterium tuberculosis. Despite its human disease relevance, sulfur metabolism in mycobacteria has not yet been fully characterized. ATP sulfurylase catalyses the synthesis of activated sulfate (adenosine 5′-phosphosulfate, APS), the first step in the reductive assimilation of sulfate. Expression of the M. tuberculosis cysD gene, predicted to encode the adenylyl-transferase subunit of ATP sulfurylase, is upregulated by the bacilli inside its preferred host, the macrophage. This study demonstrates that cysD and cysNC orthologues exist in M. tuberculosis and constitute an operon whose expression is induced by sulfur limitation and repressed by the presence of cysteine, a major end-product of sulfur assimilation. The cysDNC genes are also induced upon exposure to oxidative stress, suggesting regulation of sulfur assimilation by M. tuberculosis in response to toxic oxidants. To ensure that the cysDNC operon encoded the activities predicted by its primary sequence, and to begin to characterize the products of the operon, they were expressed in Escherichia coli, purified to homogeneity, and tested for their catalytic activities. The CysD and CysNC proteins were shown to form a multifunctional enzyme complex that exhibits the three linked catalytic activities that constitute the sulfate activation pathway.


2014 ◽  
Vol 289 (15) ◽  
pp. 10919-10929 ◽  
Author(s):  
Jonathan Herrmann ◽  
Geoffrey E. Ravilious ◽  
Samuel E. McKinney ◽  
Corey S. Westfall ◽  
Soon Goo Lee ◽  
...  

1975 ◽  
Vol 34 (03) ◽  
pp. 780-794 ◽  
Author(s):  
Dianne M Kenney ◽  
Francis C Chao ◽  
James L Tullis ◽  
Gail S Conneely

SummaryThe uptake and binding of antimitotic alkaloid colchicine has been demonstrated in washed preparations of human platelets. A silicone oil technique was adapted so that both uptake and binding of 14C-colchicine were examined in the same platelet preparations. The time dependence and amount of colchicine taken up and bound by different platelet preparations during a 90 to 120 min incubation period were highly reproducible. Both colchicine uptake and binding by intact platelets, and colchicine binding by preparations of lysed platelets were specific and temperature dependent. Colchicine uptake was slowly reversible. Magnesium and GTP enhanced colchicine binding by lysed platelet preparations but calcium decreased binding.Exposure of platelets to either cold (4° C) or to thrombin, which disrupt platelet microtubules, produced significant increases in colchicine uptake and binding. The thrombin effect was maximal at 37° C and resulted in a greater increase in uptake and binding than that produced by either cold treatment alone or, by cold treatment followed by incubation with thrombin at 37° C. The amount of increase in uptake and binding produced by thrombin was independent of both thrombin (1–5 Units/109 platelets) and colchicine concentrations (1–50 × 10−6M).It is postulated that thrombin may initiate the formation, or make available, colchicine binding sites (microtubule subunits) within platelets.


2012 ◽  
Vol 67 (2) ◽  
pp. 106-110
Author(s):  
P. Stratton
Keyword(s):  

2013 ◽  
Vol 22 (3-4) ◽  
pp. 229-254
Author(s):  
Márcio Luís Pinto Domingues

AbstractObjective:To search the English language literature for original research addressing the effect of cryotherapy on motor performance and recovery.Data Sources:We searched MEDLINE, the Physiotherapy Evidence Database, SPORT Discus, Pubmed, and the Cochrane Reviews database, from 1976 to 2009 to identify randomized clinical trials of cryotherapy, systematic reviews, original articles and methods of cryotherapy. Key words used were cryotherapy, return to participation, cold treatment, ice, injury.Data Synthesis:Brief review including assessment of cryotherapy as a tool of performance and a recovery method.Conclusions:Most studies suggest that a short rewarming time would be beneficial (a couple minutes), which is very reasonable in sports. Also, cooling techniques differ in its result accordingly to the procedures and objectives used. Finally, the type of tissue cooled plays a large role (ie. Joint vs. Muscle).


1998 ◽  
Vol 76 (7) ◽  
pp. 1188-1197 ◽  
Author(s):  
Heli M. Jutila b. Erkkilä

Seed banks of two seashore meadows were studied on the west coast of Finland (latitude 61°30'-61°33'N, longitude 21°28'-21°41'E). Samples were taken in June to a depth of 10 cm in the geolittoral zone of the grazed and ungrazed transects. The grazed samples were halved lengthwise: one half was grown immediately, the other after cold treatment. One third of the all samples was treated as controls, one third was watered with brackish water, and one third was given a pesticide treatment. Altogether, 13 926 seedlings germinated and 25 species were identified (three annuals, two biennials, and the rest perennials). Most seedlings were perennial monocots, with Juncus gerardii Loisel. the most abundant species. The seed bank was significantly larger and richer in the ungrazed site than in the grazed site. Cold treatment reduced the number of germinating species and seedlings. In the grazed and non-cold-treated samples, the numbers of species and seedlings were highest in the pesticide treatment. In ungrazed samples there were no significant differences among treatments. After the cold treatment, the least number of species and seedlings was produced by the salt-water treatment. Changing brackish water to tap water led to a burst of germination, especially of J. gerardii. The seed bank of the upper geolittoral zone was richer than that of the middle geolittoral. The multivariate classification and ordination groupings are based on the abundances of J. gerardii and Glaux maritima L.; different treatments were not distinguishable. There was a low resemblance between the seed bank and the aboveground vegetation.Key words: seed bank, salinity, pesticide, seashore meadow, cold treatment, vegetation.


2021 ◽  
Vol 13 (5) ◽  
pp. 2826
Author(s):  
Yan Tong ◽  
Hui Huang ◽  
YuHua Wang

Trihelix transcription factors play important roles in plant growth, development and various stress responses. In this study, we identified 32 trihelix family genes (DoGT) in the important Chinese medicinal plant Dendrobium officinale. These trihelix genes could be classified into five different subgroups. The gene structure and conserved functional domain of these trihelix genes were similar in the same subfamily but diverged between different subfamilies. Various stresses responsive cis-elements presented in the promoters of DoGT genes, suggesting that the trihelix genes might respond to the environmental stresses. Expressional changes of DoGT genes in three tissues and under cold treatment suggested that trihelix genes were involved in diverse functions during D. officinale development and cold tolerance. This study provides novel insights into the phylogenetic relationships and functions of the D. officinaletrihelix genes, which will aid future functional studies investigating the divergent roles of trihelix genes belonging to other species.


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