Influence of quantum dot's quantum yield to chemiluminescent resonance energy transfer

2008 ◽  
Vol 610 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Hai-Qiao Wang ◽  
Yong-Qiang Li ◽  
Jian-Hao Wang ◽  
Qiao Xu ◽  
Xiu-Qing Li ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Yield ◽  
Resonance Energy Transfer ◽  
Resonance Energy

scholarly journals Chemiluminescence resonance energy transfer–based nanoparticles for quantum yield–enhanced cancer phototheranostics

2020 ◽  
Vol 6 (21) ◽  
pp. eaaz8400 ◽  
Author(s):  
Jueun Jeon ◽  
Dong Gil You ◽  
Wooram Um ◽  
Jeongjin Lee ◽  
Chan Ho Kim ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Yield ◽  
Tumor Growth ◽  
Photochemical Reaction ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Photoacoustic Signal ◽  
Tumor Growth Inhibition ◽  
Type I ◽  
Photodynamic Therapy Of Cancer

Chemiluminescence (CL) has recently gained attention for CL resonance energy transfer (CRET)–mediated photodynamic therapy of cancer. However, the short duration of the CL signal and low quantum yield of the photosensitizer have limited its translational applications. Here, we report CRET-based nanoparticles (CRET-NPs) to achieve quantum yield–enhanced cancer phototheranostics by reinterpreting the hidden nature of CRET. Owing to reactive oxygen species (ROS)–responsive CO2 generation, CRET-NPs were capable of generating a strong and long-lasting photoacoustic signal in the tumor tissue via thermal expansion–induced vaporization. In addition, the CRET phenomenon of the NPs enhanced ROS quantum yield of photosensitizer through both electron transfer for an oxygen-independent type I photochemical reaction and self-illumination for an oxygen-dependent type II photochemical reaction. Consequently, owing to their high ROS quantum yield, CRET-NPs effectively inhibited tumor growth with complete tumor growth inhibition in 60% of cases, even with a single treatment.

Novel fluorescence resonance energy transfer optical sensors for vitamin B12 detection using thermally reduced carbon dots

2015 ◽  
Vol 39 (1) ◽  
pp. 501-507 ◽  
Author(s):  
Jilong Wang ◽  
Junhua Wei ◽  
Siheng Su ◽  
Jingjing Qiu
Keyword(s):  
Energy Transfer ◽  
Quantum Yield ◽  
Vitamin B12 ◽  
Fluorescence Resonance Energy Transfer ◽  
Optical Sensors ◽  
Carbon Dots ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fold Increase ◽  
Thermal Reduction

After thermal reduction, the quantum yield of thermally reduced carbon dots demonstrated a 5-fold increase over that of the original carbon dots.

Measuring System Calibration Factors by Unmixing the Excitation–Emission Spectra of One Dish of Cells

2021 ◽  
pp. 1-8
Author(s):  
Han Sun ◽  
Ao Yin ◽  
Lu Gao ◽  
Hongce Chen ◽  
Qilin Tang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Yield ◽  
Gaussian Function ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Emission Spectra ◽  
Measuring System ◽  
System Calibration ◽  
Statistical Frequency ◽  
Quantitative Fluorescence

Accurate predetermination of the quantum yield ratio (QA/QD) and the extinction coefficient ratio (KA/KD) between acceptor and donor is a prerequisite for quantitative fluorescence resonance energy transfer (FRET) imaging. We here propose a method to measure KA/KD and QA/QD by measuring the excitation–emission spectra (ExEm-spectra) of one dish of cells expressing m (≥3) kinds of FRET constructs. The ExEm-spectra images are unmixed to obtain the weight maps of donor (WD), acceptor (WA), and acceptor sensitization (WS). For each cell, the frequency distribution plots of the WS/WD and WS/WA images are fitted by using a single-Gaussian function to obtain the peak values of WS/WD (SD) and WS/WA (SA). The statistical frequency-SD/SA plots from all cells are fitted by using a multi-Gaussian function to obtain the peak values of both SD and SA, and then the ranges of WS/WD (RSD) and WS/WA (RSA) for each FRET construct are predetermined. Based on the predetermined RSD and RSA values of FRET constructs, our method is capable of automatically classifying cells expressing different FRET constructs. Finally, the WS/WD–WA/WD plot from different kinds of cells is linearly fitted to obtain KA/KD and QA/QD values.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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