Genetic and clinical characterization of canine leishmaniasis caused by Leishmania (Leishmania) infantum in northeastern Argentina

An entomological survey was carried out in 2007 in two Pyrenean counties of Lleida province (north-eastern Spain), where cases of autochthonous canine leishmaniasis have been recently reported. Phlebotomus ariasi and P. perniciosus, vectors of Leishmania infantum in the Mediterranean area, were captured. The aim of the present study was to compare these phlebotomine populations with others captured in known leishmaniasis foci in Europe. Populations of these species were studied by analysing the polymorphism of seven enzymatic systems (HK, PGI, PGM, MDH, 6PGD, FUM and ACO) and compared with other specimens from endemic regions of France, Italy, Malta, Portugal and Spain captured in other campaigns, and also with previously published results. Phlebotomus ariasi was more polymorphic than P. perniciosus. Only the ACO locus had diagnostic alleles, but some other alleles show high characteristic frequencies for each species. The neighbour-joining trees separated two population groups in both species. On the basis of the isoenzyme study results, sand fly populations of the Pyrenean region in Lleida province are closely related to those of other nearby leishmaniasis endemic regions in France and Spain.

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IDENTIFICATION OF Leishmania infantum IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652015000200013 ◽

2015 ◽

Vol 57 (2) ◽

pp. 175-176 ◽

Cited By ~ 9

Author(s):

Lucrecia ACOSTA ◽

Ricardo DÍAZ ◽

Pedro TORRES ◽

Gustavo SILVA ◽

Marina RAMOS ◽

...

Keyword(s):

Leishmania Infantum ◽

Public Health Problem ◽

Human Case ◽

Lutzomyia Longipalpis ◽

New Locations ◽

Misiones Province ◽

The City ◽

Northeastern Argentina ◽

Zoonotic Visceral Leishmaniasis

The emergence of zoonotic visceral leishmaniasis (ZVL) in Latin America is a growing public health problem. The urbanization of ZVL has been observed in different countries around the world, and there are a growing number of reports drawing attention to the emergence of this infection in new locations, as well as its increase in previously established areas of endemicity. In the city of Posadas, Misiones province, Northeastern Argentina, the transmission of ZVL associated with canines and Lutzomyia longipalpis was first reported in 2006. In the city of Puerto Iguazú, also in Misiones province, the first human case of ZVL was reported in February 2014. From 209 surveyed dogs, 15 (7.17%) were identified as positive by serological and/or parasitological methods. Amplification was observed in 14 samples and in all cases the species implicated was Leishmania infantum. To the authors’ knowledge, this is the first molecular characterization of L. infantum from dogs in this area.

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Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis

Acta Tropica ◽

10.1016/j.actatropica.2016.05.010 ◽

2016 ◽

Vol 161 ◽

pp. 41-43 ◽

Cited By ~ 5

Author(s):

Viviana Pinedo-Cancino ◽

Márcia Dalastra Laurenti ◽

Norival Kesper ◽

Eufrosina Setsu Umezawa

Keyword(s):

Leishmania Infantum ◽

Canine Leishmaniasis ◽

Secreted Antigens

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Genomic and Molecular Characterization of Miltefosine Resistance in Leishmania infantum Strains with Either Natural or Acquired Resistance through Experimental Selection of Intracellular Amastigotes

PLoS ONE ◽

10.1371/journal.pone.0154101 ◽

2016 ◽

Vol 11 (4) ◽

pp. e0154101 ◽

Cited By ~ 46

Author(s):

Annelies Mondelaers ◽

Maria P. Sanchez-Cañete ◽

Sarah Hendrickx ◽

Eline Eberhardt ◽

Raquel Garcia-Hernandez ◽

...

Keyword(s):

Molecular Characterization ◽

Leishmania Infantum ◽

Acquired Resistance ◽

Intracellular Amastigotes ◽

Selection Of

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Is the Prevalence of Leishmania infantum Linked to Breeds in Dogs? Characterization of Seropositive Dogs in Ibiza

Animals ◽

10.3390/ani11092579 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2579

Author(s):

Maria Edo ◽

Pablo Jesús Marín-García ◽

Lola Llobat

Keyword(s):

Immune Response ◽

Leishmania Infantum ◽

Protozoan Parasite ◽

Disease Stage ◽

Doberman Pinscher ◽

Th2 Immune Response ◽

Mediterranean Island ◽

Antibody Titers ◽

The Relationship

Leishmaniosis is an important zoonotic protozoan disease primarily spread to the Mediterranean region by Leishmania infantum, the predominant protozoan species, which accounts for the majority of cases. Development of disease depends on the immune response of the definitive host and, predictably, their genetic background. Recent studies have revealed breed-typical haplotypes that are susceptible to the spread of the protozoan parasite. The objective of this study was to analyze the prevalence of leishmaniosis on a Mediterranean island and determine the relationship between disease prevalence and breed. In addition, information on seropositive animals was recorded to characterize animals affected by the disease. To study the prevalence, a total of 3141 dogs were analyzed. Of these, the 149 infected animals were examined for age, sex, antibody titer, and disease stage. We observed a prevalence of 4.74%, which varied between breeds (p < 0.05). The Doberman Pinscher and Boxer breeds had the highest prevalence of leishmaniosis. Significant differences were observed between breeds with common ancestors, emphasizing the important genetic component. Finally, regarding the characterization of seropositive animals, the distribution is similar to other studies. We discovered a relationship (p < 0.05) between the number of antibody titers and the clinical disease stage, which was also present in Leishmania infantum, suggesting that the development of the disease depends on the humoral or Th2 immune response with ineffective antibodies.

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Identification and Evolutionary Characterization of Papillomavirus Sequences in New World Monkeys (Genera Sapajus and Alouatta) from Argentina

10.21203/rs.3.rs-994896/v1 ◽

2021 ◽

Author(s):

Candelaria Sanchez Fernandez ◽

Elisa M Bolatti ◽

Andres C.A. Culasso ◽

Diego Chouhy ◽

Martin M Kowalewski ◽

...

Keyword(s):

Pcr Amplification ◽

Pcr Primers ◽

Host Tree ◽

New World Monkeys ◽

Alouatta Caraya ◽

Captive Populations ◽

L1 Protein ◽

Viral Sequences ◽

Northeastern Argentina

Abstract Objective: In this study, we investigated the occurrence of papillomavirus (PV) infection in non-human primates (NHP, Platyrrhine) of northeastern Argentina by using broad-spectrum PCR primers at the L1 gene. In addition, we conducted a phylogenetic and coalescence analysis of viral sequences to explore their evolutionary history and evaluate the co-speciation hypothesis in the context of primate evolution. Methods: We obtained samples of 57 individuals from wild and captive populations of Alouatta caraya, Sapajus nigritus and Sapajus cay. We assessed PV infection by PCR amplification with the CUT primer system and sequencing of 337 bp (112 amino acids) of the L1 protein. The viral sequences were analyzed by phylogenetic and Bayesian coalescence methods to estimate the age of the most common recent ancestor (tMCRA) with BEAST, v1.4.8 software. We evaluated viral/host tree congruence with TreeMap v3.0. Results: We identified two novel putative PV sequences of the genus Gamma- PV in Sapajus sp and Alouatta caraya (SPV1 and AcPV1, respectively). The tMRCA of SPV1 was estimated at 11,941,682 years before present (ybp) and that of AcPV1 at 46,638,071 ybp, both predating the coalescence times of their hosts: 6.4 million years (MYA) and 6.8 MYA, respectively. Based on the comparison of primate and viral phylogenies, we could not reject the null hypothesis that the PV tree is no more congruent with the host tree than a random tree would be (P>0.05). Thus, a model of virus-host coevolution was rejected. Conclusion: This study presents the first report of PV infection in Platyrrhine species from Argentina, expands the range of described hosts for these viruses, and proposes new scenarios for their origin and dispersal.

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Characterization of a protein with unknown function (LinJ.30.3360) in Leishmania amazonensis and Leishmania infantum

Experimental Parasitology ◽

10.1016/j.exppara.2020.108048 ◽

2021 ◽

Vol 221 ◽

pp. 108048

Author(s):

Andreza Geisiane Maia Souza ◽

Ivana Helena Rocha Oliveira ◽

Aliani Moura Fonseca ◽

Thamires Queiroz-Oliveira ◽

Érica S. Martins-Duarte ◽

...

Keyword(s):

Unknown Function ◽

Leishmania Infantum ◽

Leishmania Amazonensis

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CHARACTERIZATION OF CANINE LEISHMANIASIS BY PCR-RFLP IN CUIABA, MATO GROSSO, BRAZIL

Archives of Veterinary Science ◽

10.5380/avs.v17i2.21092 ◽

2012 ◽

Vol 17 (2) ◽

Author(s):

Arleana Do Bom Parto Ferreira Almeida ◽

Daphine Ariadne Jesus de Paula ◽

Valéria Dutra ◽

Edson Moleta Colodel ◽

Luciano Nakazato ◽

...

Keyword(s):

Urban Areas ◽

Canine Leishmaniasis ◽

Prevention Measures ◽

Surrounding Area ◽

Mato Grosso ◽

Significant Difference ◽

Pcr Rflp ◽

Cutaneous Form ◽

Canine Infection

Leishmaniases are neglected zoonoses that are increasing in Brazil. The dog is considered the main reservoir of the visceral form in urban areas of Brazil and also important in maintaining the cycle of transmission of the cutaneous form in endemic areas. We used PCR-RFLP to identify the species of Leishmania involved in canine infection in Cuiaba City, Mato Grosso. Samples of bone marrow and lymph were collected from 181 dogs, of which 7.2% tested positive with indirect immunofluorescence and 24.9% using PCR-RFLP; a significant difference (p ≤ 0.05), had been possible to characterize the species Leishmania (L.) chagasi. This will aid in developing prevention measures and in the control of disease in Cuiaba and the surrounding area.

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Prokaryotic Expression and Antigenic Characterization of Three Recombinant Leishmania Antigens for Serological Diagnosis of Canine Leishmaniasis

Clinical and Diagnostic Laboratory Immunology ◽

10.1128/cdli.10.6.1153-1156.2003 ◽

2003 ◽

Vol 10 (6) ◽

pp. 1153-1156 ◽

Cited By ~ 21

Author(s):

S. Rosati ◽

M. Ortoffi ◽

M. Profiti ◽

A. Mannelli ◽

W. Mignone ◽

...

Keyword(s):

Antibody Test ◽

Canine Leishmaniasis ◽

Gene Fragment ◽

Leishmania Chagasi ◽

Serological Tests ◽

Reading Frame ◽

Antigenic Characterization ◽

Immunofluorescent Antibody Test ◽

Immunodominant Epitopes

ABSTRACT Three recombinant antigens of Leishmania chagasi (= L. infantum) were expressed in prokaryotic systems and evaluated (using a panel of dog sera characterized by parasitological and serological immunofluorescent antibody test [IFAT] techniques) as diagnostic markers of infection. The whole open reading frame encoding K9, the gene fragment encoding the repetitive sequence of K26, and the 3′-terminal gene fragment encoding a single 39-amino-acid subunit of the kinesin-related protein K39 (K39sub) were amplified from L. infantum DNA and cloned into a pGEX-2T expression vector in frame with glutathione S-transferase (GST). The sensitivity and specificity of enzyme-linked immunosorbent assays (ELISAs) using K26 as an antigen (evaluated with sera from 20 parasitologically positive and 20 parasitologically negative dogs) were both 100% (95% confidence interval [CI] = 83.2 to 100). When K9 and K39sub were used, sensitivity was 95% (95% CI = 75.1 to 99.9) and specificity was 100% (95% CI = 83.2 to 100). Using 182 field sera, a good agreement was found between the recombinant K26 ELISA and IFAT (K = 0.92; 95% CI = 0.86 to 0.98) results and between the K9 and K39sub ELISA (used in parallel) and IFAT (K = 0.87; 95% CI = 0.80 to 0.95) results. The results demonstrate that each antigen carries immunodominant epitopes and that their combination may further increase the sensitivity of currently available serological tests.

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