Vector competence of Culex tritaeniorhynchus and Culex quinquefasciatus (Diptera: Culicidae) for duck Tembusu virus transmission

Acta Tropica ◽  
2021 ◽  
Vol 214 ◽  
pp. 105785
Author(s):  
Jitra Sanisuriwong ◽  
Nichapat Yurayart ◽  
Aunyaratana Thontiravong ◽  
Sonthaya Tiawsirisup
Keyword(s):  
Culex Quinquefasciatus ◽  
Vector Competence ◽  
Virus Transmission ◽  
Culex Tritaeniorhynchus ◽  
Duck Tembusu Virus ◽  
Tembusu Virus

scholarly journals Vector competence ofAnophelesandCulexmosquitoes for Zika virus

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3096 ◽  
Author(s):  
Brittany L. Dodson ◽  
Jason L. Rasgon
Keyword(s):  
Anopheles Gambiae ◽  
Culex Quinquefasciatus ◽  
Zika Virus ◽  
Anopheles Stephensi ◽  
Vector Competence ◽  
Mosquito Species ◽  
Plaque Assay ◽  
Virus Transmission ◽  
Serious Disease ◽  
Blood Meals

Zika virus is a newly emergent mosquito-borne flavivirus that has caused recent large outbreaks in the new world, leading to dramatic increases in serious disease pathology including Guillain-Barre syndrome, newborn microcephaly, and infant brain damage. AlthoughAedesmosquitoes are thought to be the primary mosquito species driving infection, the virus has been isolated from dozens of mosquito species, includingCulexandAnophelesspecies, and we lack a thorough understanding of which mosquito species to target for vector control. We exposedAnopheles gambiae,Anopheles stephensi, andCulex quinquefasciatusmosquitoes to blood meals supplemented with two Zika virus strains. Mosquito bodies, legs, and saliva were collected five, seven, and 14 days post blood meal and tested for infectious virus by plaque assay. Regardless of titer, virus strain, or timepoint,Anopheles gambiae,Anopheles stephensi, andCulex quinquefasciatusmosquitoes were refractory to Zika virus infection. We conclude thatAnopheles gambiae,Anopheles stephensi, andCulex quinquefasciatusmosquitoes likely do not contribute significantly to Zika virus transmission to humans. However, future studies should continue to explore the potential for other novel potential vectors to transmit the virus.

Interactions of duck Tembusu virus with Aedes aegypti and Aedes albopictus mosquitoes: vector competence and viral mutation

Acta Tropica ◽  
2021 ◽  
pp. 106051
Author(s):  
Nichapat Yurayart ◽  
Patchareeporn Ninvilai ◽  
Theeraphap Chareonviriyaphap ◽  
Theerayuth Kaewamatawong ◽  
Aunyaratana Thontiravong ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Vector Competence ◽  
Duck Tembusu Virus ◽  
Viral Mutation ◽  
Tembusu Virus

scholarly journals Vector competence of Aedes aegypti and Culex quinquefasciatus from the metropolitan area of Guadalajara, Jalisco, Mexico for Zika virus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Darwin Elizondo-Quiroga ◽  
Miriam Ramírez-Medina ◽  
Abel Gutiérrez-Ortega ◽  
Armando Elizondo-Quiroga ◽  
José Esteban Muñoz-Medina ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Zika Virus ◽  
Vector Competence ◽  
Virus Transmission ◽  
Blood Feeding ◽  
Surveillance Program ◽  
Virus Spread ◽  
Zikv Infection ◽  
Almost All

AbstractZika virus (ZIKV) is a mosquito-borne pathogen discovered in the late 40’s in Uganda during a surveillance program for yellow fever. By 2014 the virus reached Eastern Island in the Americas, and two years later, the virus spread to almost all countries and territories of the Americas. The mosquito Aedes aegypti has been identified as the main vector of the disease, and several researchers have also studied the vector competence of Culex quinquefasciatus in virus transmission. The aim of the present study was to evaluate the vector competence of Ae. aegypti and Cx. quinquefasciatus in order to understand their roles in the transmission of ZIKV in Guadalajara, Jalisco, Mexico. In blood feeding laboratry experiments, we found that Ae. aegypti mosquitoes showed to be a competent vector able to transmit ZIKV in this area. On the other hand, we found that F0 Cx. quinquefasciatus mosquitoes are refractory to ZIKV infection, dissemination and transmission.

scholarly journals Detailed Analyses of Zika Virus Tropism in Culex quinquefasciatus Reveal Systemic Refractoriness

mBio ◽  
2020 ◽  
Vol 11 (4) ◽  
Author(s):  
Hannah J. MacLeod ◽  
George Dimopoulos
Keyword(s):  
Real Time ◽  
Culex Quinquefasciatus ◽  
Real Time Pcr ◽  
Zika Virus ◽  
Vector Competence ◽  
Mosquito Species ◽  
Virus Transmission ◽  
Quantitative Real Time Pcr ◽  
Content Type ◽  
Qrt Pcr

ABSTRACT The role of Culex quinquefasciatus in Zika virus transmission has been debated since the epidemic of Zika occurred in the Americas in 2015 to 2016. The majority of studies have found no evidence that C. quinquefasciatus or other Culex species are competent vectors of Zika virus, and the few studies that have proposed Zika vector status for C. quinquefasciatus have relied predominantly on quantitative real-time PCR (qRT-PCR) for viral detection. We assessed the infectious range of pre- and post-epidemic Zika virus isolates in order to classify mosquito samples based on titer infectiousness and demonstrated that two strains of C. quinquefasciatus, including one previously found to be competent, are highly resistant to infection with these Zika isolates compared to Aedes aegypti and are not competent for virus transmission. Further dissection of the dynamics of Zika exposure in both A. aegypti and C. quinquefasciatus revealed that while virus transmission by C. quinquefasciatus is blocked at the levels of the midgut and salivary glands, viral RNA persists in these tissues for prolonged periods post-exposure. We assessed Zika entry dynamics in both Aedes and Culex cells, and our results suggest that Zika virus infection in Culex cells may be blocked downstream of cell entry. These findings strongly suggest that C. quinquefasciatus is not a vector of Zika virus and additionally inform the use of qRT-PCR in vector competence assays as well as our understanding of barriers to arbovirus infection in non-susceptible mosquito species. IMPORTANCE Understanding which mosquito species transmit an emerging arbovirus is critical to effective vector control. During the Zika virus epidemic in 2015 to 2016, Aedes mosquitoes were confirmed as vectors. However, studies addressing the vector status of Culex quinquefasciatus mosquitoes presented conflicting evidence and remain an outstanding source of confusion in the field. Here, we established a robust cell-based assay to identify infectious titers of Zika virus and assessed the virus titers in C. quinquefasciatus by quantitative real-time PCR (qRT-PCR). We found that while low levels of virus were detected in C. quinquefasciatus, these titers did not correspond to infectious virus, and these mosquitoes did not transmit virus in the saliva. We also present evidence that the virus may enter Culex cells before infection is disrupted. Our findings are important for future studies incriminating vector species using qRT-PCR for virus detection and offer new information on how virus transmission is blocked by mosquitoes.

scholarly journals Cloning, Analysis and Anti-Duck Tembusu Virus Innate Immune Response of Cherry Valley Duck TRIM32

2021 ◽  
pp. 101048
Author(s):  
Tianxu Li ◽  
Xiaofang Hu ◽  
Tingting Zhang ◽  
Xingdong Song ◽  
Huihui Zhang ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Duck Tembusu Virus ◽  
Cherry Valley Duck ◽  
Tembusu Virus

Detection of antibodies to duck tembusu virus in human population with or without the history of contact with ducks

2021 ◽  
Author(s):  
Rojjanaporn Pulmanausahakul ◽  
Kunjimas Ketsuwan ◽  
Thitigun Jaimipuk ◽  
Duncan R. Smith ◽  
Prasert Auewarakul ◽  
...  
Keyword(s):  
Human Population ◽  
Duck Tembusu Virus ◽  
History Of ◽  
Tembusu Virus

scholarly journals Duck Tembusu virus exhibits neurovirulence in BALB/c mice

2013 ◽  
Vol 10 (1) ◽  
pp. 260 ◽  
Author(s):  
Shuang Li ◽  
Xiaoxia Li ◽  
Lijiao Zhang ◽  
Yongyue Wang ◽  
Xiuling Yu ◽  
...  
Keyword(s):  
Duck Tembusu Virus ◽  
Tembusu Virus

scholarly journals Sequential Infection of Aedes aegypti Mosquitoes with Chikungunya Virus and Zika Virus Enhances Early Zika Virus Transmission

Insects ◽  
2018 ◽  
Vol 9 (4) ◽  
pp. 177 ◽  
Author(s):  
Tereza Magalhaes ◽  
Alexis Robison ◽  
Michael Young ◽  
William Black ◽  
Brian Foy ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Blood Meal ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Vector Competence ◽  
Virus Transmission ◽  
Mosquito Vector ◽  
Molecular Aspects ◽  
Virus Interactions ◽  
Sequential Infection

In urban settings, chikungunya, Zika, and dengue viruses are transmitted by Aedes aegypti mosquitoes. Since these viruses co-circulate in several regions, coinfection in humans and vectors may occur, and human coinfections have been frequently reported. Yet, little is known about the molecular aspects of virus interactions within hosts and how they contribute to arbovirus transmission dynamics. We have previously shown that Aedes aegypti exposed to chikungunya and Zika viruses in the same blood meal can become coinfected and transmit both viruses simultaneously. However, mosquitoes may also become coinfected by multiple, sequential feeds on single infected hosts. Therefore, we tested whether sequential infection with chikungunya and Zika viruses impacts mosquito vector competence. We exposed Ae. aegypti mosquitoes first to one virus and 7 days later to the other virus and compared infection, dissemination, and transmission rates between sequentially and single infected groups. We found that coinfection rates were high after sequential exposure and that mosquitoes were able to co-transmit both viruses. Surprisingly, chikungunya virus coinfection enhanced Zika virus transmission 7 days after the second blood meal. Our data demonstrate heterologous arbovirus synergism within mosquitoes, by unknown mechanisms, leading to enhancement of transmission under certain conditions.

Sign in / Sign up

Export Citation Format

Share Document