Nitric oxide modulates metalloproteinase-2, collagen deposition and adhesion rate after polypropylene mesh implantation in the intra-abdominal wall

2012 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Ana I.S. Moretti ◽  
Francisco J.P. Souza Pinto ◽  
Vivian Cury ◽  
Marcia C. Jurado ◽  
Wagner Marcondes ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Collagen Deposition ◽  
Mesh Implantation ◽  
Adhesion Rate

BIOMECHANICAL PROPERTIES OF MESHES FOLLOWING IMPLANTATION IN THE RAT ABDOMINAL WALL MODEL

2016 ◽  
Vol 16 (03) ◽  
pp. 1650036
Author(s):  
NATASHA UDPA ◽  
SHAMA IYER ◽  
SEAN P. MCDONOUGH ◽  
YINGXIN GAO
Keyword(s):  
Mechanical Strength ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Biomechanical Properties ◽  
Uniaxial Tensile ◽  
Collagen Deposition ◽  
Collagen Types ◽  
Wall Model ◽  
Uniaxial Tensile Testing ◽  
Significant Difference

The objective of our study was to (1) evaluate mesh strength and collagen incorporation after 4 and 12 weeks of implantation in a rat abdominal wall model and (2) determine the relationship between collagen deposition and mechanical strength of a chitosan-coated polypropylene mesh. We implanted 0.5% chitosan-coated polypropylene mesh (PPM), collagen-coated PPM (PelvitexTM; C.R. Bard), and PPM (Avaulta Solo[Formula: see text]; C.R. Bard) using a rat abdominal defect model. Mechanical properties were determined from uniaxial tensile testing and collagen deposition of each mesh was evaluated 4 and 12 weeks post-implantation. We found that after implantation, the neo tissue of Ch-PPM is stiffer than the commercially available meshes. We also observed no significant difference in the ratio of collagen types I/III between mesh samples at 4 weeks or 12 weeks. We found no relationship between the ratio of collagen types I/III and the mechanical strength of mesh samples after implantation. The increased stiffness with chitosan coating could be due to increased muscle tissue ingrowth.

scholarly journals Fibroplasia after polypropylene mesh implantation for abdominal wall hernia repair in rats

2009 ◽  
Vol 24 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Márcia Vaz ◽  
Rodrigo Ketzer Krebs ◽  
Eduardo Neubarth Trindade ◽  
Manoel Roberto Maciel Trindade
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Type I Collagen ◽  
Giant Cells ◽  
Type Iii Collagen ◽  
Type I ◽  
Mesh Implantation ◽  
Time Required ◽  
Post Implantation ◽  
Time Point

PURPOSE: This study assessed the collagen deposition and correlated it with local inflammatory responses to evaluate the length of time required for fibroplasia when polypropylene meshes are used to repair incisional abdominal wall hernias in rats. METHODS: Thirty-six male Wistar rats underwent longitudinal resection of a peritoneal and musculoaponeurotic tissue segment (3x2 cm) of the abdominal wall followed by defect reconstruction with polypropylene mesh bridging over aponeurosis. The animals were divided into 6 groups according to the time points for the analysis of fibroplasia: 1, 2, 3, 7, 21 and 30 days post-implantation. Animals were sacrificed at each time point, and the site where the polypropylene mesh was implanted was evaluated histologically to assess inflammatory response and percentage of collagen using computer-assisted videomorphometry. RESULTS: Total collagen was found at the mesh site on the 3rd day post-implantation, and increased progressively on all subsequent days up to the 21st day, when it reached its highest percentage (p<0.001). Type III collagen increased progressively from the 3rd to the 21st days, when it reached its highest percentage (p<0.001); on the 30th day, it decreased significantly (p>0.001). Type I collagen was first found between the 7th and 21st days; it reached its highest percentage on the 21st day and then remained stable until the 30th day. The type I to type III collagen ratio increased significantly and progressively up to the 30th day (p<0.001). Neutrophils were found at the mesh site from the 1st to the 21st day post-implantation. Macrophages, giant cells and lymphocytes were seen on the 2nd day. Thirty days after mesh implantation, neutrophils disappeared, but the percentages of macrophages, giant cells and lymphocytes remained stable (p<0.001). CONCLUSIONS: This study showed that total collagen was first seen on the 3rd day post-implantation, with a higher percentage of type I collagen at the last observational time point. The prolonged healing inflammatory response and the persistence of chronic inflammation surrounding to the mesh did not affect the length of time required for fibroplasia.

scholarly journals Evaluation of peritoneal adhesions formation and tissue response to polypropylene - poli (2-hydroxyethyl methacrylate)-(polyHEMA) implant on rats' abdominal wall

2010 ◽  
Vol 25 (4) ◽  
pp. 337-341 ◽  
Author(s):  
Neusa Margarida Paulo ◽  
Sonia Maria Malmonge ◽  
Liliana Borges de Menezes ◽  
Flávia Gontijo de Lima ◽  
Aline de Moraes Faria ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Histopathological Examination ◽  
Adhesion Formation ◽  
Foreign Body Response ◽  
Tissue Response ◽  
Female Rats ◽  
Hydroxyethyl Methacrylate ◽  
Peritoneal Adhesions ◽  
Body Response

PURPOSE: To verify if the composit poli (2-hydroxyethyl methacrylate)-PolyHEMA/polypropylene mesh implanted in the female rat's abdominal wall could be suitable for the prevention of peritoneal adhesions, and for the evaluation of the tecidual response produced by this biomaterial. METHODS: Polypropylene meshes (Group PP, n=20) and polypropylene meshes coated with a layer of poli (2-hydroxyethyl methacrylate)-PolyHEMA (Group PH, n=20) were implanted on the abdominal wall of Wistar female rats. Ten animals from each group were submitted to euthanasia at 15 and 30 days of the postoperative period. RESULTS: The animals from the group PP presented visceral adhesions on the mesh surface, which was not observed in the ones from group PH. At the histopathological examination foreign body response was observed in both groups, whilst there was a greater intensity of inflammatory response in group PH on both moments. CONCLUSION: The poli (2-hydroxyethyl methacrylate) polyHEMA hydrogel associated to polypropylene mesh reduces visceral adhesion formation in rats, although it may be associated to greater inflammatory reaction.

scholarly journals Evaluations of Implanted Polypropylene Mesh After Surgical Removal Due to Eventration or Mesh Rejection

2017 ◽  
Vol 54 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Bogdan Stelian Mastalier Manolescu ◽  
Valentin Popescu ◽  
Marius Septimiu Petrutescu ◽  
Andrada Serafim ◽  
Izabela Cristina Stancu
Keyword(s):  
Gold Standard ◽  
Polypropylene Mesh ◽  
Microscopic Level ◽  
Surrounding Tissue ◽  
Surgical Removal ◽  
Postoperative Evaluation ◽  
Mesh Implantation ◽  
Individual Variations ◽  
The Right ◽  
Mesh Rejection

The Gold standard in parietal wall hernias is represented by mesh implantation. Both wall repair and hernia-associated complications are related to the anatomic location and biomechanics of the structures involved specificity. This work reports the results of the intraoperative and postoperative evaluation of synthetic polypropylene mesh integration. The mesh and the surrounding tissue, previously removed and preserved, have been analyzed macroscopically and at microscopic level. The tissue invaded the pores of the mesh and coated the monofilaments. The right balance between the mesh type and tissular response is hard to evaluate preoperatively, due to individual variations, but we can upgrade the criteria used to personalize the treatment for the best possible outcome.

scholarly journals The optical analysis of the abdominal wall using the biospeckle after implants of polypropylene mesh in rats

2009 ◽  
Vol 24 (6) ◽  
pp. 442-448
Author(s):  
Tâmara Maria Nieri ◽  
Marco Antonio de Oliveira Peres ◽  
Emerson Rodrigo da Silva ◽  
Inácio Maria Dal Fabbro ◽  
Mikiya Muramatsu ◽  
...  
Keyword(s):  
Animal Model ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Male Rats ◽  
Mesh Placement ◽  
Group 4 ◽  
Mesh Prosthesis ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

PURPOSE: To evaluate an experimental animal model to study the abdominal tissue activity considering its interaction with a polypropylene mesh, through the use of one of the optical phenomena of light Laser, the biospeckle. METHODS: Fifty Wistar male rats were divided into four groups: Group 1: ten animals not submitted to surgery; Group 2: ten animals submitted to surgery without polypropylene mesh; Group 3: 20 animals submitted to surgery followed by the mesh placement; Group 4: (sham) with ten animals. None of the animals presented post surgical complications being submitted to the optical tests at the 20th postoperative day. RESULTS: The analysis from the biospeckle tests, comparing the medians and standard deviations with T Student test, indicated that no significative difference was observed on the abdominal wall tissue activity in the four groups considered, with and without polypropylene mesh prosthesis implantation. CONCLUSION: The animal model is viable and the biospeckle open ways for a great number of experiments to be developed in evaluating tissue activity.

Tissue Reaction to Polypropylene Mesh: A Study of Oedema, Blood Flow, and Inflammation in the Abdominal Wall

1991 ◽  
Vol 23 (3-4) ◽  
pp. 240-249 ◽  
Author(s):  
S. Dabrowiecki ◽  
K. Svanes ◽  
J. Lekven ◽  
K. Grong
Keyword(s):  
Blood Flow ◽  
Abdominal Wall ◽  
Polypropylene Mesh ◽  
Tissue Reaction
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