scholarly journals I-Wire Heart-on-a-Chip I: Three-dimensional cardiac tissue constructs for physiology and pharmacology

2017 ◽  
Vol 48 ◽  
pp. 68-78 ◽  
Author(s):  
Veniamin Y. Sidorov ◽  
Philip C. Samson ◽  
Tatiana N. Sidorova ◽  
Jeffrey M. Davidson ◽  
Chee C. Lim ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Three Dimensional ◽  
Tissue Constructs

Three-Dimensional Bioprinted Functional and Contractile Cardiac Tissue Constructs

2019 ◽  
Vol 229 (4) ◽  
pp. e59
Author(s):  
Sang J. Lee ◽  
John D. Jackson ◽  
James J. Yoo ◽  
Anthony Atala
Keyword(s):  
Cardiac Tissue ◽  
Three Dimensional ◽  
Tissue Constructs

scholarly journals Polymeric Biomaterials for the Treatment of Cardiac Post-Infarction Injuries

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1038
Author(s):  
Sonia Trombino ◽  
Federica Curcio ◽  
Roberta Cassano ◽  
Manuela Curcio ◽  
Giuseppe Cirillo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Polymeric Nanoparticles ◽  
Three Dimensional ◽  
Cardiac Regeneration ◽  
Biological Properties ◽  
Positive Influence ◽  
Regenerative Process ◽  
Current Status ◽  
Polymeric Biomaterials

Cardiac regeneration aims to reconstruct the heart contractile mass, preventing the organ from a progressive functional deterioration, by delivering pro-regenerative cells, drugs, or growth factors to the site of injury. In recent years, scientific research focused the attention on tissue engineering for the regeneration of cardiac infarct tissue, and biomaterials able to anatomically and physiologically adapt to the heart muscle have been proposed as valuable tools for this purpose, providing the cells with the stimuli necessary to initiate a complete regenerative process. An ideal biomaterial for cardiac tissue regeneration should have a positive influence on the biomechanical, biochemical, and biological properties of tissues and cells; perfectly reflect the morphology and functionality of the native myocardium; and be mechanically stable, with a suitable thickness. Among others, engineered hydrogels, three-dimensional polymeric systems made from synthetic and natural biomaterials, have attracted much interest for cardiac post-infarction therapy. In addition, biocompatible nanosystems, and polymeric nanoparticles in particular, have been explored in preclinical studies as drug delivery and tissue engineering platforms for the treatment of cardiovascular diseases. This review focused on the most employed natural and synthetic biomaterials in cardiac regeneration, paying particular attention to the contribution of Italian research groups in this field, the fabrication techniques, and the current status of the clinical trials.

Abstract 020: Cell-sheet-based Bioengineered Human Cardiac Tissue Using Pluripotent Stem Cells For Heart Repair And Disease Models

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Katsuhisa Matsuura ◽  
Tatsuya Shimizu ◽  
Nobuhisa Hagiwara ◽  
Teruo Okano
Keyword(s):  
Cardiac Tissue ◽  
Subcutaneous Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Embryoid Bodies ◽  
Cardiac Differentiation ◽  
Cardiac Cell ◽  
Cell Sheets ◽  
High Efficient ◽  
Human Cardiac Tissue

We have developed an original scaffold-free tissue engineering approach, “cell sheet engineering”, and this technology has been already applied to regenerative medicine of various organs including heart. As the bioengineered three-dimensional cardiac tissue is expected to not only function for repairing the broad injured heart but also to be the practicable heart tissue models, we have developed the cell sheet-based perfusable bioengineered three-dimensional cardiac tissue. Recently we have also developed the unique suspension cultivation system for the high-efficient cardiac differentiation of human iPS cells. Fourteen-day culture with the serial treatments of suitable growth factors and a small compound in this stirring system with the suitable dissolved oxygen concentration produced robust embryoid bodies that showed the spontaneous beating and were mainly composed of cardiomyocytes (~80%). When these differentiated cells were cultured on temperature-responsive culture dishes after the enzymatic dissociation, the spontaneous and synchronous beating was observed accompanied with the intracellular calcium influx all over the area even after cell were detached from culture dishes as cell sheets by lowering the culture temperature. The cardiac cell sheets were mainly composed of cardiomyocytes (~80%) and partially mural cells (~20%). Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid, and this propagation was inhibited by the treatment with some anti-arrhythmic drugs. When the triple layered cardiac tissue was transplanted onto the subcutaneous tissue of nude rats, the spontaneous pulsation was observed over 2 months and engrafted cardiomyocytes were vascularized with the host tissue-derived endothelial cells. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue. Now we are developing the vascularized thickened human cardiac tissue by the repeated layering of cardiac cell sheets on the artificial vascular bed in vitro.

Bioprinting in ophthalmology: current advances and future pathways

2019 ◽  
Vol 25 (3) ◽  
pp. 496-514 ◽  
Author(s):  
Nataraj Poomathi ◽  
Sunpreet Singh ◽  
Chander Prakash ◽  
Rajkumar V. Patil ◽  
P.T. Perumal ◽  
...  
Keyword(s):  
3D Printing ◽  
Cardiac Tissue ◽  
Human Life ◽  
Three Dimensional ◽  
3D Models ◽  
Eye Diseases ◽  
Biological Research ◽  
Content Type ◽  
Almost All ◽  
Time Of Operation

Purpose Bioprinting is a promising technology, which has gained a recent attention, for application in all aspects of human life and has specific advantages in different areas of medicines, especially in ophthalmology. The three-dimensional (3D) printing tools have been widely used in different applications, from surgical planning procedures to 3D models for certain highly delicate organs (such as: eye and heart). The purpose of this paper is to review the dedicated research efforts that so far have been made to highlight applications of 3D printing in the field of ophthalmology. Design/methodology/approach In this paper, the state-of-the-art review has been summarized for bioprinters, biomaterials and methodologies adopted to cure eye diseases. This paper starts with fundamental discussions and gradually leads toward the summary and future trends by covering almost all the research insights. For better understanding of the readers, various tables and figures have also been incorporated. Findings The usages of bioprinted surgical models have shown to be helpful in shortening the time of operation and decreasing the risk of donor, and hence, it could boost certain surgical effects. This demonstrates the wide use of bioprinting to design more precise biological research models for research in broader range of applications such as in generating blood vessels and cardiac tissue. Although bioprinting has not created a significant impact in ophthalmology, in recent times, these technologies could be helpful in treating several ocular disorders in the near future. Originality/value This review work emphasizes the understanding of 3D printing technologies, in the light of which these can be applied in ophthalmology to achieve successful treatment of eye diseases.

A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation

2019 ◽  
Vol 25 (4) ◽  
pp. 243-252 ◽  
Author(s):  
Bai Yang ◽  
Cecillia Lui ◽  
Enoch Yeung ◽  
Hiroshi Matsushita ◽  
Anjana Jeyaram ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Three Dimensional

scholarly journals Computer modelling and simulation of a novel printing head for complex tissue engineering constructs

2020 ◽  
Vol 318 ◽  
pp. 01045
Author(s):  
Gokhan Ates
Keyword(s):  
Tissue Engineering ◽  
Computer Modelling ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Biological Properties ◽  
Functionally Graded ◽  
Mixing Efficiency ◽  
Tissue Constructs ◽  
Multiple Materials ◽  
Graded Structures

In tissue engineering, three-dimensional functional scaffolds with tailored biological properties are needed to be able to mimic the hierarchical structure of biological tissues. Recent developments in additive biomanufacturing allow to extrude multiple materials enabling the fabrication of more sophisticated tissue constructs. These multi-material biomanufacturing systems comprise multiple printing heads through which individual materials are sequentially printed. Nevertheless, as more printing heads are added the fabrication process significantly decreases, since it requires mechanical switching among the physically separated printheads to enable printing multiple materials. In addition, this approach is not able to create biomimetic tissue constructs with property gradients. To address these limitations, this paper presents a novel static mixing extrusion printing head to enable the fabrication of multi-material, functionally graded structures using a single nozzle. Computational fluid dynamics (CFD) was used to numerically analyze the influence of Reynolds number on the flow pattern of biomaterials and mixing efficiency considering different miscible materials.

Bioengineered Muscle Implants

2000 ◽  
Author(s):  
G. L. Bowlin ◽  
Barbara Wise ◽  
L. Terracio ◽  
D. G. Simpson
Keyword(s):  
Injection Site ◽  
Cardiac Tissue ◽  
Treatment Options ◽  
Three Dimensional ◽  
Small Animal ◽  
Scar Tissue ◽  
Specific Domain ◽  
Complete Replacement ◽  
Cell Based Therapy ◽  
And Function

Abstract Fundamental research has defined many of the mechanistic events that mediate congenital malformations and the pathological disease processes that alter cardiac structure and function. Despite these efforts, there are a limited number of clinical treatment options available for many of these conditions. In many cases, even for disease processes that cause focal defects in the ventricular wall, the only viable treatment is the complete replacement of the damaged organ by transplant. Unfortunately, the supply of cardiac tissue that is available for transplant therapy remains chronically, and critically, short of demand. The reconstruction of a specific domain of dysfunctional ventricular tissue with a cell-based therapy is a potential avenue of treatment. One of the most direct strategies in this type of treatment regime is the injection of a suspension of fetal or neonatal cardiac myocytes into the injured domain. In small animal models, two limitations have become apparent with this strategy. First, differentiated myocytes do not undergo migration when they are injected into scar tissue and as a result they tend to remain concentrated in the vicinity of the injection site. Second, the myocytes that survive in the injection site are not well integrated into the healthy tissue and contract at rates that are independent of the surrounding myocardium. The long-term objective of this project is to circumvent the limitations of injection therapy by fabricating a cardiac muscle prosthesis that mimics the three dimensional architecture of the intact heart.

scholarly journals Three‐dimensional bioprinting human cardiac tissue chips of using a painting needle method

2019 ◽  
Vol 116 (11) ◽  
pp. 3136-3142 ◽  
Author(s):  
Shohei Chikae ◽  
Akifumi Kubota ◽  
Haruka Nakamura ◽  
Atsushi Oda ◽  
Akihiro Yamanaka ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Three Dimensional ◽  
Human Cardiac Tissue
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