Efficacy of exogenous hormone (GnRHa) for induced breeding of climbing perch Anabas testudineus (Bloch, 1792) and influence of operational sex ratio on spawning success

2016 ◽  
Vol 171 ◽  
pp. 114-120 ◽  
Author(s):  
Babita Mandal ◽  
Rajesh Kumar ◽  
P. Jayasankar
Keyword(s):  
Sex Ratio ◽  
Operational Sex Ratio ◽  
Exogenous Hormone ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Spawning Success

DOMESTIFIKASI IKAN BETOK Anabas testudineus MELALUI PEMIJAHAN DI WADAH BUDIDAYA (CLIMBING PERCH, Anabas testudineus DOMESTICATION THROUGH SPAWNING)

2016 ◽  
Vol 2 (4) ◽  
pp. 188
Author(s):  
Untung Bijaksana ◽  
Septiodrian R Balantek
Keyword(s):  
Rainy Season ◽  
Gonadal Development ◽  
Water Levels ◽  
Natural State ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Latent Time ◽  
Egg Diameter ◽  
Fish Gonads ◽  
Spawning Success

<p>Penelitian ini dimaksudkan sebagai upaya domestikasi Ikan Betok melalui pemijahan dalam wadah budidaya. Penelitian ini dilakukan di Laboratorium Basah Program Studi Budidaya Perairan Universitas Lambung Mangkurat . Tiga poin utama dalam mendukung keberhasilan pemijahan ikan, yaitu : perkembangan gonad , ovulasi dan pemijahan . Tentu perkembangan panjat bertengger gonad ikan terjadi pada awal musim kemarau puncak musim dan di awal musim hujan atau saat musim hujan , pemijahan terjadi . Keadaan alami ini mencoba untuk diadopsi dalam budidaya kontainer , dengan fashting selama 1 , 2 dan 3 hari dengan tingkat air , 10 , 15 dan 20 cm . Hasilnya adalah waktu yang laten dari 11,7 jam sampai 39 jam , fekunditas antara 5310-7376 butir , diameter telur 0,5 hingga 0,8 mm , tingkat fertilisasi antara 88,2-93,2 persen dan tingkat menetas antara 87 , 0-91,6 persen.</p><p>The study is intended as an attempt domestication through climbing perch spawning in the container cultivation. The research was conducted at the Laboratory of Wetlands Program University Faculty of Fisheries Aquaculture Lambung Mangkurat. Three main points in support of the spawning success of fish, namely: gonadal development, ovulation and spawning. Naturally development climbing perch fish gonads occurred in the early dry season to peak season and at the beginning of the rainy season or during the rainy season, spawning occurred. This natural state is trying to be adopted in the cultivation of container, with fashting for 1, 2 and 3 days with water levels, 10, 15 and 20 cm. The result is a latent time of 11.7 hours to 39 hours, fecundity between 5310 to 7376 grains, egg diameter 0.5 to 0.8 mm, the degree of fertilization between 88.2 to 93.2 percent and the degree of hatching between 87, 0 to 91.6 percent.</p>

scholarly journals BIOLOGI REPRODUKSI IKAN BETOK (Anabas testudineus) DI PAPARAN BANJIRAN LUBUK LAMPAM,KABUPATEN OGAN KOMERING ILIR

2015 ◽  
Vol 6 (3) ◽  
pp. 137
Author(s):  
Eko Prianto ◽  
Mohammad Mukhlis Kamal ◽  
Ismudi Muchsin ◽  
Endi Setiadi Kartamihardja
Keyword(s):  
Sex Ratio ◽  
Anabas Testudineus ◽  
Commercial Fish ◽  
Male And Female ◽  
Climbing Perch ◽  
Male Sex ◽  
First Maturity ◽  
Egg Diameter ◽  
Floodplain Area ◽  
Somatic Index

Ikan betok (Anabas testudineus) adalah salah satu jenis ikan ekonomis penting yang dihasilkan dari perairan paparan banjiran. Penelitian yang bertujuan untuk mengkaji beberapa aspek biologi reproduksi ikan betok di paparan banjiran Lubuk Lampam, Kabupaten Ogan Komering Ilir dilakukan pada bulan Nopember 2012-Oktober 2013. Sampel ikan ditangkap setiap bulanmenggunakan alat tangkap jaring dan bengkirai.Analisis data meliputi sebaran frekuensi ukuran panjang, nisbah kelamin, tingkat kematangan gonad, indeks kematangan gonad, ukuran pertamakali matang gonad, potensi reproduksi dan pola reproduksi. Jumlah sampel ikan betok yang diperoleh sebanyak 540 ekor, terdiri dari 187 ekor ikan jantan dan 353 ekor ikan betina, dengan kisaran panjang ikan betina antara 27-224 mm dan ikan jantan antara 48-243 mm. Rasio kelamin ikan jantan dan betina adalah 0,53 : 1. Tingkat kematangan gonad (TKG) ikan jantan dan betina yang paling banyak ditemui adalah TKG I dengan frekuensi tertinggi pada ukuran 116-132 mm dan 114-129 mmmasing-masing berjumlah 34 dan 33 ekor. Perkembangan tingkat kematangan gonad ikan betok dipengaruhi oleh perubahan tinggimuka air secara musiman. Indek kematangan gonad ikan jantan pada TKGIV berkisar 1,3-15,0%dan ikan betina berkisar antara 1,2 17,1%. Ukuran pertama kali matang gonad ikan betina adalah pada panjang total 160 mm dan ikan jantan pada panjang total 177 mm. Fekunditas ikan betok berkisar antara 224–182.736 butir dengan diameter telur berkisar antara 0,465-1,026 mmdengan pola pemijahan secara sebagian. Climbing perch, Anabas testudineus is a dominant commercial fish inhabit floodplain area of Lubuk Lampam, Ogan Komering Ilir Regency. A study aimed to investigate some aspects of the reproductive biology of climbing perch has been conducted at floodplain of Lubuk Lampam, Ogan Komering Ilir regency from November 2012 to October 2013. Fish sampling was conducted every month using nets and bamboo trap. The data analysis includes lenght frequency distribution, sex ratio, the gonado maturity, gonado somatic index, the size at first maturity, fecundity and reproductive patterns. Climbing perch sample amounted of 540 specimen compose of 187 males and 353 females, with the lenght frequency between 27-224 mm (female) and 48-243 mm (male). Sex ratio of the male and female of the climbing perch was 0,53 : 1. The gonado maturity of male and female are mostly at the first level with the highest frequency between 116-132 mm and 114-129 mm, equivalent to 34 and 33 specimen, respectively. The development of gonado maturity of climbing perch was influenced by seasonally of water level fluctuation. Gonado somatic index of maturity of male and female range 1.3-15.0% and 1.2 -17.1%, respectively. The size at the first maturity of the female was 160 mm lenght and of the male was 177 mm lenght. The fecundity ranges 224 to182,736 eggs with the egg diameter ranges 0.465-1.026 mm and the climbing perch was classified into partially spawner.

scholarly journals Pembetinaan ikan betok, Anabas testudineus (Bloch, 1792) menggunakan larutan susu dan kedelai melalui perendaman larva

2018 ◽  
Vol 17 (2) ◽  
pp. 123
Author(s):  
Helmi Zuryani ◽  
Boby Muslimin ◽  
Khusnul Khotimah
Keyword(s):  
Sex Ratio ◽  
Fish Larvae ◽  
Cow’S Milk ◽  
Chemical Laboratory ◽  
Anabas Testudineus ◽  
Cow's Milk ◽  
Climbing Perch ◽  
Material Sample ◽  
The University ◽  
Biology Laboratory

The ability of climbing perch, Anabas testudineus (Bloch, 1792) to grow effectively can be done for a sex reversal using natural steroids active ingredient which are derived from cow’s milk through immersion of the fish larvae. This research aims to increase the ratio of the female sex, survival, and growth of the larvae of climbing perch. The research material is a juvenile of 7 days old which is gained from induce breeding methods. The research was conducted at a hatchery. Sex ratio was observed in biology laboratory and water quality was observed in chemical laboratory of the University of Muhammadiyah Palembang from March to May 2016. Post-larva was immersed with three media for the treatment i.e. cow’s milk (P1), soya milk (P2) and the mix (P3). The immersion was done by 10 hours; used 2 ml L-1 dozed on each part and 50 post-larva were immersed on jar sized 10 liters. After immersing the post-larva was reared during 60 days in aquarium sized 30x30x30 cm3 for observation of sex ratio and growth. Sampling method used is a random sampling. The post-larva and water were observed every 10 days with 40% material sample of post-larva. The result showed that best grow is in P1 (61.11%). The length growth is 3.77 cm, biomass growth is 1.69 g, and produce sex ratio of female is 77.78%. AbstrakKemampuan ikan betok, Anabas testudineus (Bloch, 1792) untuk tumbuh secara efektif dapat dilakukan dengan pembe-tinaan menggunakan bahan aktif steroid alami yang berasal dari susu sapi melalui perendaman larva. Penelitian ini ber-tujuan untuk meningkatkan nisbah kelamin betina, sintasan, dan pertumbuhan larva ikan betok. Bahan uji pada peneliti-an ini adalah larva usia tujuh hari yang didapatkan dari hasil pemijahan induk dengan penyuntikan secara semi alami. Tempat pemeliharaan larva dilakukan di panti benih. Penentuan nisbah kelamin larva ikan dilakukan di laboratorium biologi dan pengamatan air diuji di Laboratorium Kimia Fakultas Pertanian Universitas Muhammadiyah Palembang dari bulan Maret sampai bulan Mei 2016. Perlakuan uji dilakukan dengan perendaman larva pada media, yaitu susu sapi (P1), susu kedelai (P2), dan campuran keduanya (P3). Masing-masing perlakuan direndam selama 10 jam dengan dosis perlakuan sebesar 2ml L-1 dan larva sebanyak 50 ekor L-1direndam pada stoples ukuran 10 liter. Setelah proses peren-daman, larva dipelihara dalam akuarium ukuran 30x30x30 cm3 selama 60 hari untuk diamati nisbah kelamin dan tingkat pertumbuhannya. Metode sampling yang digunakan adalah pengambilan sampel secara acak. Sampling pada ikan uji dan air diamati setiap 10 hari sekali dengan jumlah sampling sebanyak 40% pada ikan. Hasil penelitian menunjukkan perlakuan perendaman dengan larutan susu sapi adalah yang tertinggi, sintasan larva ikan betok sebesar 61,11%, per-tumbuhan panjang sebesar 3,77 cm, pertumbuhan bobot sebesar 1,69 g, dan nisbah kelamin betina sebesar 77,78% .

scholarly journals Isolation and characterization of two virulent Aeromonads associated with haemorrhagic septicaemia and tail-rot disease in farmed climbing perch Anabas testudineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Abhishek Mazumder ◽  
Hrishikesh Choudhury ◽  
Abhinit Dey ◽  
Dandadhar Sarma
Keyword(s):  
Natural Infection ◽  
Winter Season ◽  
Pcr Amplification ◽  
Body Parts ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Isolation And Characterization ◽  
Biochemical Characterisation ◽  
Rot Disease

AbstractDiseased Anabas testudineus exhibiting signs of tail-rot and ulcerations on body were collected from a fish farm in Assam, India during the winter season (November 2018 to January 2019). Swabs from the infected body parts were streaked on sterilized nutrient agar. Two dominant bacterial colonies were obtained, which were then isolated and labelled as AM-31 and AM-05. Standard biochemical characterisation and 16S rRNA and rpoB gene sequencing identified AM-31 isolate as Aeromonas hydrophila and AM-05 as Aeromonas jandaei. Symptoms similar to that of natural infection were observed on re-infecting both bacteria to disease-free A. testudineus, which confirmed their virulence. LC50 was determined at 1.3 × 104 (A. hydrophila) and 2.5 × 104 (A. jandaei) CFU per fish in intraperitoneal injection. Further, PCR amplification of specific genes responsible for virulence (aerolysin and enterotoxin) confirmed pathogenicity of both bacteria. Histopathology of kidney and liver in the experimentally-infected fishes revealed haemorrhage, tubular degeneration and vacuolation. Antibiotic profiles were also assessed for both bacteria. To the best of our knowledge, the present work is a first report on the mortality of farmed climbing perch naturally-infected by A. hydrophila as well as A. jandaei, with no records of pathogenicity of the latter in this fish.

scholarly journals Sex Chromosome Meiotic Drive in Stalk-Eyed Flies

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1169-1180 ◽  
Author(s):  
Daven C Presgraves ◽  
Emily Severance ◽  
Gerald S Willrinson
Keyword(s):  
Sex Ratio ◽  
Sex Chromosome ◽  
Sex Ratios ◽  
Natural Populations ◽  
Meiotic Drive ◽  
Operational Sex Ratio ◽  
Genetic Modifiers ◽  
Ratio Distortion ◽  
The Impact ◽  
Sex Ratio Distortion

Meiotically driven sex chromosomes can quickly spread to fixation and cause population extinction unless balanced by selection or suppressed by genetic modifiers. We report results of genetic analyses that demonstrate that extreme female-biased sex ratios in two sister species of stalk-eyed flies, Cyrtodiopsis dalmanni and C. whitei, are due to a meiotic drive element on the X chromosome (Xd). Relatively high frequencies of Xd in C. dalmanni and C. whitei (13–17% and 29%, respectively) cause female-biased sex ratios in natural populations of both species. Sex ratio distortion is associated with spermatid degeneration in male carriers of Xd. Variation in sex ratios is caused by Y-linked and autosomal factors that decrease the intensity of meiotic drive. Y-linked polymorphism for resistance to drive exists in C. dalmanni in which a resistant Y chromosome reduces the intensity and reverses the direction of meiotic drive. When paired with Xd, modifying Y chromosomes (Ym) cause the transmission of predominantly Y-bearing sperm, and on average, production of 63% male progeny. The absence of sex ratio distortion in closely related monomorphic outgroup species suggests that this meiotic drive system may predate the origin of C. whitei and C. dalmanni. We discuss factors likely to be involved in the persistence of these sex-linked polymorphisms and consider the impact of Xd on the operational sex ratio and the intensity of sexual selection in these extremely sexually dimorphic flies.

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