53P Expression of sodium-dependent phosphate transporter NaPi2b is downregulated in malignant ovarian tumors after neoadjuvant chemotherapy

Phosphorus is known as a key element associated with growth, energy, and cell signaling. In plants, phosphate transporters (PHTs) are responsible for moving and distributing phosphorus in cells and organs. PHT genes have been genome-wide identified and characterized in various plant species, however, these genes have not been widely identified based on available genomic data in Camellia sativa, which is an important oil seed plant. In the present study, we found 66 PHT genes involved in phosphate transporter/translocate in C. sativa. The recognized genes belonged to PHTs1, PHTs2, PHTs4, PHOs1, PHO1 homologs, glycerol-3-PHTs, sodium dependent PHTs, inorganic PHTs, xylulose 5-PHTs, glucose-6-phosphate translocators, and phosphoenolpyruvate translocators. Our finding revealed that PHT proteins are divers based on their physicochemical properties such as Isoelectric point (pI), molecular weight, GRAVY value, and exon-intron number(s). Besides, the expression profile of PHT genes in C. sativa based on RNA-seq data indicate that PHTs are involved in response to abiotic stresses such as cold, drought, salt, and cadmium. The tissue specific expression high expression of PHO1 genes in root tissues of C. sativa. In additions, four PHTs, including a PHT4;5 gene, a sodium dependent PHT gene, and two PHO1 homolog 3 genes were found with an upregulation in response to aforementioned studied stresses. In the current study, we found that PHO1 proteins and their homologs have high potential to post-translation modifications such as N-glycosylation and phosphorylation. Besides, different cis-acting elements associated with response to stress and phytohormone were found in the promoter region of PHT genes. Overall, our results show that PHT genes play various functions in C. Sativa and regulate Camellia responses to external and intracellular stimuli. The results can be used in future studies related to the functional genomics of C. sativa.

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Novel Immunogenic Epitopes in the NaPi-IIb Protein: Production of Monospecific Antibodies Using Synthetic Peptides Outlined on Isoform Specific Regions of the Type IIb Sodium-Dependent Phosphate Transporter (NaPi-IIb)

Journal of Cancer Therapy ◽

10.4236/jct.2016.72015 ◽

2016 ◽

Vol 07 (02) ◽

pp. 129-151

Author(s):

Ângela Alice Amadeu Megale ◽

Jorge Mário da Costa Ferreira Júnior ◽

Letícia Batista Azevedo Rangel ◽

Maria Del Pilar Estevez Diz ◽

Max Senna Mano ◽

...

Keyword(s):

Synthetic Peptides ◽

Protein Production ◽

Phosphate Transporter ◽

Sodium Dependent ◽

Monospecific Antibodies

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Role of the sodium-dependent phosphate transporter PIT1 in bone growth in vivo

Bone ◽

10.1016/j.bone.2011.03.171 ◽

2011 ◽

Vol 48 ◽

pp. S107

Author(s):

A. Bourgine ◽

P. Pilet ◽

S. Diouani ◽

S. Sourice ◽

J. Lesoeur ◽

...

Keyword(s):

Bone Growth ◽

Phosphate Transporter ◽

Sodium Dependent

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Lipid peroxidation in tissues of ovarian tumors after neoadjuvant chemotherapy.

Journal of Clinical Oncology ◽

10.1200/jco.2017.35.15_suppl.e17036 ◽

2017 ◽

Vol 35 (15_suppl) ◽

pp. e17036-e17036

Author(s):

Oksana E. Kravtsova ◽

Galina Andreevna Nerodo ◽

Vera P. Nikitina ◽

Ekaterina V. Verenikina ◽

Ivan Nikitin ◽

...

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Neoadjuvant Chemotherapy ◽

Tumor Tissue ◽

Ovarian Tissue ◽

Ovarian Tumors ◽

Benign Tumors ◽

Sod Activity ◽

Diene Conjugates ◽

Copper Zinc

e17036 Background: The purpose of the study was to analyze neoadjuvant chemotherapy (NACT) effect on parameters of monoaminergic, free radical and hydrolytic systems in tissues of malignant ovarian tumors. Methods: Activity of parameters of monoaminergic, free radical and hydrolytic systems was determined in ovarian tissue of 92 patients with ovarian cancer. Tissues of tumorous and contralateral (non-tumorous) ovaries were studied, and results were compared to the data in 30 patients with uterine fibroids and other benign tumors. Results: Tumorous ovaries showed the decrease in vitamins E and A by 2.6 and 4.1 times, respectively, compared to the norm. The levels in contralateral ovaries were 5 and 2.8 times lower than the norm, respectively. SOD in tumor tissue exceeded the norm by 46%. Content of copper-zinc-SOD amounted to 15.7% of the total activity in healthy ovaries, while in tumorous ones – 8.2%. Diene conjugates in tumors were increased by 42% compared to the healthy ovaries. Contralateral ovaries showed 2.1 times increase in SOD activity compared to the norm; catalase activity was decreased by 35% which was similar to the value in tumor tissue. Diene conjugates in contralateral ovaries were increased by 38% compared to the healthy ovaries and did not differ from the value in tumor. MDA level was decreased by 4.1 times and did not differ from the value in tumor. Tumorous ovaries after NACT showed an increase in vitamins A and E by 1.8 and 3.2 times, respectively, compared to the levels before treatment; copper-zinc-SOD activity increased by 40.3%. Lipid peroxidation changed as well: diene conjugates level increased by 24.7% and MDA decreased by 47.7% compared to the values before treatment. Levels of the main antioxidant enzymes – SOD and catalase – in contralateral ovaries after NACT were similar to the normal ones; diene conjugates were close to the norm, vitamin E decreased by 40%, vitamin A – by 30%, MDA content did not change. Conclusions: Morphologically healthy tissue of the contralateral ovary in the organism affected by malignant tumor demonstrates signs of imbalance in antioxidant factors similar to those in the tumorous ovary. NACT results in normalization of metabolism indices in both tumorous and contralateral ovaries.

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