Influence of dentin pre-treatment with NaOCl on the morphology of adhesive interface of self-etching adhesive systems

Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.

Download Full-text

Does tin pre-treatment enhance the bond strength of adhesive systems to enamel?

Journal of Dentistry ◽

10.1016/j.jdent.2013.03.009 ◽

2013 ◽

Vol 41 (7) ◽

pp. 642-652 ◽

Cited By ~ 7

Author(s):

Nadine Schlueter ◽

Anne Peutzfeldt ◽

Carolina Ganss ◽

Adrian Lussi

Keyword(s):

Bond Strength ◽

Adhesive Systems ◽

Pre Treatment

Download Full-text

Quantitative and qualitative evaluation of resin-dentin bond strengths: in vitro effects of surface treatment with metalloproteinase inhibitors

Journal of Research in Dentistry ◽

10.19177/jrd.v2e32014251-260 ◽

2014 ◽

Vol 2 (3) ◽

pp. 251

Author(s):

Marcelo Totti ◽

Marcelo Goulart ◽

Laerte Ohse Fagundes ◽

Thaís Thomé ◽

Ewerton Nochi Conceição ◽

...

Keyword(s):

Bond Strength ◽

Qualitative Evaluation ◽

Adhesive System ◽

Layer Formation ◽

Adhesive Systems ◽

Hybrid Layer ◽

Mmp Inhibitor ◽

Pre Treatment ◽

In Vitro Effects

AIM: The objective of this study was to evaluate the use of MMPs inhibitors (chlorhexidine and EDTA) in bond strength and quality of the hybrid layer of adhesive restorations in normal dentin using two ethanol-based total-etch adhesive systems. MATERIAL AND METHODS: Thirty-two extracted human molars were coronally sectioned and randomly divided into 8 groups (n=4), depending on the surface pre-treatment and adhesive system used. The total-etch adhesive systems Single Bond 2 (2-step) and Adper Scotchbond Multi-Purpose Plus (3-step) were used as follows: 1) according to manufacturer's instructions (etching with 37% phosphoric acid (H3PO4) for 15 s); 2) etching with H3PO4 for 15 s, followed by 2% chlorhexidine for 120 s; 3) etching with 0.1 M EDTA for 60 s; 4) etching with 0.1 M EDTA followed by 2% chlorhexidine for 120 s. Teeth were incrementally restored with composite resin (Filtek Z350XT). After water storage for 24 h, teeth were double-sectioned, yielding stick specimens of 1.0 mm² bonded area, and then subjected to microtensile bond strength (MTBS) test at 0.5 mm/min. Additional specimens were gold-sputtered to be analyzed under scanning electron microscopy (SEM). Data (in Mega Pascal) were subjected to Kruskal-Wallis and Dunn (p <0.05) tests. RESULTS: The etching protocol (37% H3PO4 or EDTA) interfered with hybrid layer formation, monomer penetration and the MTBS. Funnel shaped resin tags were observed when dentin was etched with 37% H3PO4. In these specimens, MTBS were also higher. EDTA conditioning produced thin hybrid layers and smaller MTBS, regardless the adhesive system used. Chlorhexidine application after conditioning resulted in no apparent differences between both evaluated techniques (37% H3PO4 or EDTA). CONCLUSION: The use of chlorhexidine as a MMP inhibitor does not alter immediate bond strength values and does not interfere with hybrid layer formation.

Download Full-text

Quantitative comparison of permeability in the adhesive interface of four adhesive systems

The Journal of Korean Academy of Conservative Dentistry ◽

10.5395/jkacd.2009.34.1.051 ◽

2009 ◽

Vol 34 (1) ◽

pp. 51

Author(s):

Juhea Chang ◽

Keewook Yi ◽

Hae-Young Kim ◽

In Bog Lee ◽

Byeong Hoon Cho ◽

...

Keyword(s):

Quantitative Comparison ◽

Adhesive Systems ◽

Adhesive Interface

Download Full-text

In vitro Study of the Structure and Adhesive Interface in Direct Restorations with Experimental Nanocomposite Materials and Adhesive Systems

Materiale Plastice ◽

10.37358/mp.18.4.5088 ◽

2018 ◽

Vol 55 (4) ◽

pp. 620-625

Author(s):

Anca Porumb ◽

Adrian Almasi ◽

Liana Todor ◽

Cristian Adrian Ratiu ◽

Gabriela Ciavoi ◽

...

Keyword(s):

Clinical Importance ◽

In Vitro Study ◽

Adhesive System ◽

Nanocomposite Materials ◽

Variable Number ◽

Adhesive Systems ◽

Adhesive Interface ◽

Chemistry Research ◽

Self Etch

Using nanotechnology to produce composite materials it has been possible to obtain materials with similar or better mechanical properties than previous generation of microhybrids, and with exceptional aesthetics and chandelier due to the particularities of nanomaterials. At the same time, the improvement of adhesive techniques to enamel and dentin allowed the expansion of the area of direct composite restorations with a better prognostic over time.Eight carie-free molars were used and each of them was prepared with two class II cavities, which were filled using the C13 and C20 nanocomposites and the A1 and A2 experimental adhesive systems prepared at the Raluca Ripan Chemistry Research Institute. The teeth were divided into four groups of two teeth each, depending on the adhesive system and the composite material used. Scanning electron microscopy images (SEM) have described the adhesive interface and structure characteristics for the two nanocomposite materials and for the two studied adhesive systems.The studied nanocomposites have a variable number of porosities and air bubbles, with a clinical importance which is yet hard to establish.The experimental adhesives used produce a good interface and infiltration of the smear-layer but have not been visualized inside the dentinal canals.In vitro studies are necessary to evaluate the durability of adhesion in the case of self-etch adhesives in one or two steps.

Download Full-text

MICROLEAKAGE PATTERN AT TOOTH-ADHESIVE INTERFACE UNDER METAL-BRACKETS BONDED WITH CONVENTIONAL OR FLOWABLE NANO-ADHESIVE SYSTEMS

Egyptian Dental Journal ◽

10.21608/edj.2017.75031 ◽

2017 ◽

Vol 63 (1) ◽

pp. 815-821

Author(s):

Rania Badawy ◽

Nabil Abou-Shamaa ◽

Walid Elsayed

Keyword(s):

Adhesive Systems ◽

Adhesive Interface

Download Full-text

10-MDP Based Dental Adhesives: Adhesive Interface Characterization and Adhesive Stability—A Systematic Review

Materials ◽

10.3390/ma12050790 ◽

2019 ◽

Vol 12 (5) ◽

pp. 790 ◽

Cited By ~ 19

Author(s):

Eunice Carrilho ◽

Miguel Cardoso ◽

Manuel Marques Ferreira ◽

Carlos Marto ◽

Anabela Paula ◽

...

Keyword(s):

Systematic Review ◽

Chemical Interaction ◽

Adhesive System ◽

Cochrane Library ◽

Adhesion Forces ◽

Adhesive Systems ◽

Acid Base ◽

Functional Monomers ◽

Adhesive Interface ◽

Adhesion Stability

The incorporation of functional monomers in dental adhesive systems promotes chemical interaction with dental substrates, resulting in higher adhesion forces when compared to micromechanical adhesion only. The 10-MDP monomer, whose chemical structure allows for a polar behavior which is favorable to adhesion, also promotes the protection of collagen fibers through the formation of MDP-calcium salts. This systematic review aimed to characterize the interface created by 10-MDP containing adhesive systems through an evaluation of the following parameters: Formation of nano-layered structures, capacity to produce an acid-base resistant zone, and adhesion stability. The research was conducted using PubMed, Cochrane Library, Web of Science and Embase, limited to English, Spanish, and Portuguese articles. The research was done according to the PICO strategy. The 10-MDP monomer has the capacity to produce an acid-base resistant zone on the adhesive interface, which increases the response to acid-base challenges. The adhesion established by these systems is stable over time. To have the best of these adhesive solutions, a scrubbing technique must be used to apply the adhesive system on dental substrates, in order to improve monomers infiltration and to create a stable bond. Time must be given for the solution to infiltrate, hybridize and form the MDP-Ca, improving adhesive stability.

Download Full-text

Morphological characterization of the tooth/adhesive interface

Brazilian Dental Journal ◽

10.1590/s0103-64402006000300001 ◽

2006 ◽

Vol 17 (3) ◽

pp. 179-185 ◽

Cited By ~ 6

Author(s):

Sandra Kiss Moura ◽

José Fortunato Ferreira Santos ◽

Rafael Yagüe Ballester

Keyword(s):

Composite Resin ◽

Morphological Characteristics ◽

Adhesive System ◽

Morphological Characterization ◽

Single Bond ◽

Third Molars ◽

Adhesive Systems ◽

Adhesive Interface ◽

Etch And Rinse

The purpose of this study was to assess the morphological characteristics of the tooth/adhesive interface using different adhesive systems in MOD restorations under scanning electron microscopy (SEM). The tested hypothesis was that the morphology of the bonding interface would vary in different areas of MOD restorations for the three adhesive systems. MOD cavities were prepared in 12 sound extracted human third molars and restored with Filtek Z250 composite resin and one of the following adhesive systems: Experimental ABF (n=4), Clearfil SE Bond (n=4) self-etching primers and Single Bond etch-and-rinse adhesive system (n=4). After 24-h storage in distilled water at 37ºC, teeth were sectioned and prepared for SEM. The interfacial morphology varied depending on the adhesive system and also on the evaluated area. The null hypothesis was accepted because the morphology of the tooth/adhesive interface reflected the characteristics of both the dental substrate and the adhesive systems.

Download Full-text