Facile preparation of an electrochemical aptasensor based on Au NPs/graphene sponge for detection of homocysteine

2021 ◽  
pp. 149735
Author(s):  
Xiao-Hong Wen ◽  
Xue-Feng Zhao ◽  
Bo-Fang Peng ◽  
Kai-Ping Yuan ◽  
Xiao-Xi Li ◽  
...  
2020 ◽  
pp. 1-8
Author(s):  
Mohadeseh Safaei ◽  
Hadi Beitollahi ◽  
Masoud Reza Shishehbore

The present research aimed to detect the cytochrome C using methylene blue (MB)-anchored ultrasensitive electrochemical aptamer conjugated with Au nanoparticles (NPs), as a new electrochemical sandwich immunoassay. Aptamer-Au NPs could act as sandwich amplification element and MB accumulation reagent. According to the results, the interaction of MB and guanine occurred strongly on aptamer probe. After switching the aptamer structure by the cytochrome C, the MB- labeled aptamer probe was forced to detach from the sensing interface. Moreover, the cytochrome C concentrations (0.05-10.0 µM) with 5.0 nM detection limit reduced linearly the peak current of MB. In conclusion, an acceptable selectivity was observed for the constructed aptasensor in detecting the cytochrome C.


2020 ◽  
Vol 16 (8) ◽  
pp. 1296-1303
Author(s):  
Xiaojuan Xia ◽  
Mei Li ◽  
Min Wang ◽  
Meng-Qiao Gu ◽  
Kuan-Neng Chi ◽  
...  

Ochratoxin is teratogenic, carcinogenic and immunotoxic to humans. It contains ochratoxin in many foods, so the detection of ochratoxin in food is particularly important. In this paper, gold nanoparticles (Au NPs)@g-C3N4 composite was synthesized by loading Au NPs on carbon nitride material, and it was immobilized on the surface of glassy carbon electrode by chitosan (Chit) as the substrate of electrochemical aptasensors. An ochratomycin A electrochemical aptasensor was constructed by hybridizing DNA1 and ochratoxin A (OTA) aptamers. The resulting hybrid strands were immobilized on the substrate of glassy carbon electrode. Electrochemical alternating current impedance (EIS) was used to detect the impedance value of the aptasensor when incubating different concentrations of OTA. The impedance value is inversely ratio to the concentration of OTA, achieving quantitative detection of OTA. The aptasensor can detect OTA with a linear range of 0.5–100 ng/mL, the linear correlation coefficient is 0.9506, and the detection limit is 0.167 ng/mL. This aptasensor provides a novel and efficient method for detecting OTA.


2018 ◽  
Vol 29 (23) ◽  
pp. 235706 ◽  
Author(s):  
Y Y Liu ◽  
X L Guo ◽  
L Zhao ◽  
L Zhu ◽  
Z T Chen ◽  
...  

TAPPI Journal ◽  
2015 ◽  
Vol 14 (3) ◽  
pp. 167-174 ◽  
Author(s):  
QIANQIAN WANG ◽  
J.Y. ZHU

Mixed office paper (MOP) pulp without deinking with an ash content of 18.1 ± 1.5% was used as raw material to produce nanofiller-paper. The MOP pulp with filler was mechanically fibrillated using a laboratory stone grinder. Scanning electron microscope imaging revealed that the ground filler particles were wrapped by cellulose nanofibrils (CNFs), which substantially improved the incorporation of filler into the CNF matrix. Sheets made of this CNF matrix were densified due to improved bonding. Specific tensile strength and modulus of the nanofiller-paper with 60-min grinding reached 48.4 kN·m/kg and 8.1 MN·m/kg, respectively, approximately 250% and 200% of the respective values of the paper made of unground MOP pulp. Mechanical grinding duration did not affect the thermal stability of the nanofiller-paper.


Author(s):  
Dorian Bader ◽  
Johannes Fröhlich ◽  
Paul Kautny

The facile preparation of three regioisomeric thienopyrrolocarbazoles applying a convenient C-H activation approach is presented. Derived from indolo[3,2,1-<i>jk</i>]carbazole, the incorporation of thiophene into the triarylamine framework significantly impacted the molecular properties of the parent scaffold. The developed thienopyrrolocarbazoles enrich the family of triarylamine donors and constitute a novel building block for functional organic materials.


Sign in / Sign up

Export Citation Format

Share Document