scholarly journals Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu (Hamilton, 1822)

2018 ◽  
Vol 11 ◽  
pp. 59-69 ◽  
Author(s):  
Mathialagan Kavitha ◽  
Manickam Raja ◽  
Pachiappan Perumal
Keyword(s):  
Freshwater Fish ◽  
Digestive Tract ◽  
Probiotic Potential ◽  
Bacillus Spp

scholarly journals pH and Temperature Dependent Gut Enzyme Niche in a Stomachless Herbivorous Freshwater Fish Amblypharyngodon mola (Hamilton, 1822)

2020 ◽  
Vol 12 (4) ◽  
pp. 729-741
Author(s):  
S. Debnath ◽  
S. I. Maiti ◽  
S. K. Saikia
Keyword(s):  
Bile Salt ◽  
Freshwater Fish ◽  
Digestive Tract ◽  
Hierarchical Clustering ◽  
Standard Length ◽  
Active Regions ◽  
Temperature Dependent ◽  
Optimum Ph ◽  
Temperature Levels ◽  
Optimum Ph And Temperature

The activities of digestive α-amylase (E. C. 3.2.1.1), total proteases, and bile salt-activated lipase (E. C. 3.1.1.-) along the digestive tract (lengthwise divided into five equal parts) of a stomachless freshwater fish (n = 10, weight = 4.354±0.316 g, standard length = 21.641±2.271 cm) were measured at different pH and temperature levels. Different optimum pH and temperature for the activity of α-amylase (8-9, 35°C), proteases (7-8, 45°C), and lipase (8, 45°C) were observed. The first two regions of the digestive tract showed comparatively higher activity of all enzymes. The hierarchical clustering technique revealed three different enzymatically active regions, more inclined to pH in the digestive tract of the studied fish. The present study also supports that the stomachless gut of A. mola has substantial resemblances to the intestinal part of the digestive tract of fish.

scholarly journals Natural Endotoxemia in Dogs—A Hidden Condition That Can Be Treated with a Potential Probiotic Containing Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici: A Study Model

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1367
Author(s):  
Maria-Catalina Matei ◽  
Sanda Maria Andrei ◽  
Victoria Buza ◽  
Mihai Sorin Cernea ◽  
Daria Antonia Dumitras ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Pediococcus Acidilactici ◽  
Probiotic Potential ◽  
New Information ◽  
Probiotic Treatment ◽  
Before And After ◽  
Bacillus Spp ◽  
Group 2 ◽  
Group 1

Spore-based Bacillus spp. products are considered to have a higher probiotic potential compared to products containing only lactic acid bacteria because their viability in the gastrointestinal (GI) tract is higher, even when GI environmental conditions are unfavorable. The aim of this study was to assess the effect of a Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici spore-based potential probiotic on the natural levels of postprandial endotoxemia. A total of 11 dogs completed the study: group 1—healthy dogs: n = 5; group 2—dogs with apparent dysbiosis: n = 6. For 30 days, the dogs were fed the probiotic product; clinical examinations and blood sampling were done before and after completion of the probiotic treatment. Endotoxin levels were assessed pre-meal, 6 h and 12 h post-meal, before initiation and after completion of the treatment. The results showed a decrease in endotoxin levels after treatment, especially 12 h post-meal (group 1: 20.60%; group 2: 44.93%). This study reports new information with regard to natural endotoxemia levels in dogs and suggests that a multi-strain formula (spore-based) consisting of B. subtilis, B. licheniformis and P. acidilactici is able to diminish endotoxin values.

scholarly journals The internal CO 2 threat to fish: high P CO 2 in the digestive tract

2019 ◽  
Vol 286 (1907) ◽  
pp. 20190832 ◽  
Author(s):  
Chris M. Wood ◽  
Junho Eom
Keyword(s):  
Climate Change ◽  
Rainbow Trout ◽  
Freshwater Fish ◽  
Digestive Tract ◽  
Venous Blood ◽  
External Environment ◽  
Current Concern ◽  
Anterior Intestine ◽  
The Mean

Our goal was to use novel fibreoptic sensors to make the first direct P CO 2 measurements in the digestive tracts of live freshwater fish (anaesthetized, artificially ventilated, 12°C). P CO 2 levels in gastrointestinal fluids were substantially higher than in blood, and were elevated after feeding. In the carnivorous, gastric rainbow trout, the mean P CO 2 in various parts of the tract increased from 7–13 torr (1 torr = 0.1333 kPa) during fasting to 20–41 torr after feeding, relative to arterial levels of 3.5–4 torr. In the agastric, omnivorous goldfish, the mean gut levels varied from 10–13 torr in fasted animals to 14–18 torr in fed animals, relative to arterial levels of 5–7 torr. These elevated P CO 2 values were associated with surprisingly high HC O 3 − concentrations (greater than 40 mmol l −1 ) in the intestinal chyme. Incubations of food pellets with acid or water revealed endogenous P CO 2 generation sufficient to explain gastric P CO 2 in fed trout and anterior intestine P CO 2 in fed goldfish. The impacts of possible equilibration with venous blood draining the tract are assessed. We conclude that fish are already coping with P CO 2 levels in the internal gastrointestinal environment many-fold greater than those of current concern in the external environment for climate change and aquacultural scenarios.

PARASITES OF FRESHWATER FISH: V. PARASITIC HELMINTHS OF THE MUSKALLUNGE, ESOX M. MASQUINONGY MITCHILL, IN THE ST. LAWRENCE WATERSHED

1951 ◽  
Vol 29 (4) ◽  
pp. 290-295 ◽  
Author(s):  
L. P. E. Choquette
Keyword(s):  
Freshwater Fish ◽  
Digestive Tract ◽  
Parasitic Helminths ◽  
Leptorhynchoides Thecatus ◽  
Triaenophorus Nodulosus

The incidence of the following species of helminths recovered from the digestive tract of 218 muskallunge, Esox m. masquinongy, from various localities in the St. Lawrence watershed is recorded: Azygia augusticauda, A. longa, Triaenophorus nodulosus, Proteocephalus pinguis, Neoechinorhynchus cylindratus, Leptorhynchoides thecatus, Metabronema salvelini, and Rhaphidascaris canadensis. One hundred and ninety-two, or 88% of the fish examined were found to harbor one or more species. The most commonly found species were T. nodulosus and A. longa. In all cases the number of worms recovered per host was small.

scholarly journals PROBIOTIC POTENTIAL OF Bacillus cereus AGAINST Vibrio spp. IN POST-LARVAE SHRIMPS

2018 ◽  
Vol 31 (2) ◽  
pp. 495-503 ◽  
Author(s):  
JULIANA MARIA ADERALDO VIDAL ◽  
MAURÍCIO NOGUEIRA DA CRUZ PESSÔA ◽  
FERNANDO LEANDRO DOS SANTOS ◽  
PAULO DE PAULA MENDES ◽  
MIKO SHINOZAKI MENDES
Keyword(s):  
Weight Gain ◽  
Bacillus Cereus ◽  
Probiotic Bacteria ◽  
Beneficial Effects ◽  
Antimicrobial Substances ◽  
Probiotic Potential ◽  
Histopathological Lesions ◽  
Significant Difference ◽  
Bacillus Spp ◽  
Lower Weight Gain

ABSTRACT Bacillus spp. have been used against diseases caused by bacteria that affect cultured shrimp, providing beneficial effects on the host shrimps by altering their microbial community, and improving zootechnical indexes. The objective of this work was to evaluate the effects of a diet supplemented with Bacillus cereus-a bacterium with probiotic potential-on post-larvae Litopenaeus vannamei shrimps grown in laboratory. The experiment lasted for fifteen days and consisted of six treatments-control (T1), probiotic (T2), Vibrio parahaemolyticus (VP) (T3), probiotic and VP (T4), V. alginolyticus (VA) (T5), and probiotic and VA (T6). The survival rate, weight gain, colonization capacity of the probiotic bacteria, pathogen count, and histopathological lesions were evaluated. There was no significant difference (p=0.05) in survival between treatments. The groups with pathogens and without probiotics presented lower weight gain. The result of the Bacillus cereus count in the treatments T2, T4 and T6 were significantly different (p<0.05), the probiotic bacteria were more aggressive in competing for space and nutrients when compared to V. parahaemolyticus than when compared to V. alginolyticus. Animals fed with the probiotic presented lower counts of these pathogens than those fed without the probiotic (p<0.05). No histopathological lesions were found in the organs and tissues of the shrimps. Bacillus cereus showed a high colonizing capacity in post-larvae shrimps, causing a significant reduction of pathogens, probably by secreting antimicrobial substances and the competitive exclusion, which justifies their use as probiotic bacteria.

scholarly journals Description of the cultivable bacterioma of the digestive tract and gills of Oreochromis niloticus and isolation of bacteria with probiotic potential

2020 ◽  
Vol 7 (5) ◽  
pp. 14-20
Author(s):  
MOROH Jean-Luc Aboya ◽  
KARAMOKO Detto ◽  
KOKORA Aya Philomène ◽  
ANGOUA Amanahan Mauricette Prisca ◽  
YAO Ange Olivier Parfait ◽  
...  
Keyword(s):  
Digestive Tract ◽  
Oreochromis Niloticus ◽  
Probiotic Potential

scholarly journals High-Quality Genome Sequence Assembly of R.A73 Enterococcus faecium Isolated from Freshwater fish mucus Exhibiting high Probiotic Potential

2020 ◽  
Author(s):  
Rim El Jeni ◽  
Kais Ghedira ◽  
Monia El Bour ◽  
Sonia Abdelhak ◽  
Alia Ben Kahla ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Genome Sequencing ◽  
Enterococcus Faecium ◽  
Genome Annotation ◽  
Whole Genome ◽  
Industrial Manufacturing ◽  
Probiotic Potential ◽  
Genome Sequence Assembly ◽  
Fish Mucus ◽  
High Quality Genome

Abstract Background: Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that promote health. Genome sequencing and functional genomics investigations of LABs varieties provide rapid and important information about their diversity and their evolution, revealing a significant molecular basis.This study investigates the whole genome sequences of the Enterococcus faecium genome (HG937697), isolated from the mucus of freshwater fish in Tunisian dams. Genomic DNA was extracted using the Quick-GDNA kit (Zymoresearch) and sequenced using the Illumina HiSeq2500 system. Sequences quality assessment was performed using FastQC software. The complete genome annotation was carried out with the RAST web server then NCBI PGAAP. Results: The Enterococcus faecium R.A73 assembled in 28 contigs consisting of 2,935,283 bps. The genome annotation revealed 2,884 genes in total including 2,834 coding sequences and 50 RNAs containing 3 rRNAs (one rRNA 16s, one rRNA 23s and one rRNA 5s) and 47 tRNAs. Twenty-two genes implicated in bacteriocin production are identified within the Enterococcus faecium R.A73 genome.Conclusions: Data obtained provide insights to further investigate the effective strategy for testing this Enterococcus faecium R.A73 strain in the industrial manufacturing process. Studying their metabolism with bioinformatics tools represents the future challenge and contribution to improving the utilization of the multi-purpose bacteria in food.

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