scholarly journals Description of the cultivable bacterioma of the digestive tract and gills of Oreochromis niloticus and isolation of bacteria with probiotic potential

Author(s):  
MOROH Jean-Luc Aboya ◽  
KARAMOKO Detto ◽  
KOKORA Aya Philomène ◽  
ANGOUA Amanahan Mauricette Prisca ◽  
YAO Ange Olivier Parfait ◽  
...  
2018 ◽  
Vol 5 (2) ◽  
pp. 129-139
Author(s):  
Devi Craselly Sihombing ◽  
Ade Dwi Sasanti ◽  
Mohamad Amin

ABSTRACTSynbiotics are a balanced combination of probiotics and prebiotics. Synbiotics indirectly influence the population of bacteria in the digestive tract and survival of the fish. The purpose of this research is to determine the affection of synbiotic addition to diet for population of intestinal bacteria, feed efficiency, survival rate and growth of tilapia. This research conducted on October-December 2016 at Laboratorium Budidaya Perairan, Departement of Aquaculture, Faculty of Agriculture, Sriwijaya. This research methode used Completly Randomize Design with six treatments and three replications. Tilapia feed treated with different dose of sweet potato extract prebiotics. P0 treatment by feeding (commercial pellets without probiotics and prebiotics), P1 treatment (5 ml probiotic + 2,5 ml prebiotic/kg pellets), P2 treatment (5 ml probiotic + 5,0 ml prebiotic/kg pellets), P3 treatment (5 ml probiotic + 7,5 ml prebiotic/kg pellets), P4 treatment (5 ml probiotic + 10 ml prebiotic/kg pellets) and P5 treatment (5 ml probiotic + 12,5 ml prebiotic/kg pellets). Parameters to be observed is population of intestinal bacteria, feed efficiency, growth and survival rate of tilapia. The highest value population of intestinal bacteria is P5 treatment with 4,17x104 cfu/ml. P3 treatment has the highest value of feed efficiency 50,19%, growth of length 1,92 cm, growth of weight 8,87 gram and survival rate 88,89%. Keywords: synbiotic, probiotic, prebiotic, feed.


Author(s):  
Bessie Evelyn Oliva-Hernández ◽  
Francis Mariel Santos-Ruiz ◽  
Manuel Alejandro Muñoz-Wug ◽  
Juan Francisco Pérez-Sabino

Microplastics are plastic particles smaller than 5 mm found in the environment, which can cause health problems for aquatic organisms and humans, being classified as emerging pollutants. In Guatemala, Lake Amatitlán is one of the most polluted lakes as it receives discharges of wastewater, treated and untreated, as well as other waste from Guatemala City and other major urban populations. In a recent study, microplastics were found in sediments in Lake Amatitlán, so it was necessary to determine whether the lake's fish are affected by these pollutants, which can be harmful to their health. This research aimed to determine the microplastics in fish from Lake Amatitlán, for which 65 specimens of Nile tilapia (Oreochromis niloticus) were collected in October and December 2020, and January 2021. The collected fish were dissected in the laboratory, where the number and type of microplastics were analyzed in the digestive tract, after their separation by digestion and filtration. Lines or fibers were the most common type of microplastics, found in 63 specimens (96.9% of the analyzed specimens), ranging from 0 to 27 lines/fibers per individual. The frequency of other types of microplastics found were 0-3 fragments/individual, 0-2 films/individual, and 0-4 foams/individual, while microspheres were not found in any specimen. The presence of microplastics in tilapia from Lake Amatitlán is an indicator of contamination in the lake by pollutants that could affect the trophic network and represents a risk for the fish consumers, requiring the attention of environmental and health authorities. Keywords: emerging pollutants, Guatemala, pollution.


Author(s):  
Vagne Melo Oliveira ◽  
Caio Rodrigo Dias Assis ◽  
Helane Maria Silva Costa ◽  
Raquel Pereira Freitas Silva ◽  
Juliana Ferreira Santos ◽  
...  

2020 ◽  
Vol 2 (1) ◽  
pp. 23-31
Author(s):  
Alpina Bukhori ◽  
Sartini Sartini ◽  
Rahmiati Rahmiati

Lactic acid bakteria have characteristics that are able to ferment sugars or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this research was to isolates the lactic acid bacteria from the digestive tract of tilapia fish and to investigate the inhibitory power produced by lactic acid bacteria in inhibiting the growth of Staphylococcus aureus and Shigella sp. Isolation of lactic acid bacteria was done by using MRSA medium and to see the ability of lactic acid bacteria isolates in inhibiting the growth of Staphylococcus aureus and Shigella sp then used disc diffusion method. The isolates obtained were then characterized by morphology and biochemistry. From the research obtained two lactic acid bacteria that is sp1 and sp2. Lactic acid bacteria sp1 and sp2 are able to inhibit Staphylococcus aureus and Shigella sp with the biggest inhibition to Staphylococcus aureus by sp2 of 8.75 mm and the biggest inhibition of Shigella sp is shown by sp2 that is equal to 7.16 mm. Based on the results of isolation and characterization done in this research can be concluded that lactic acid bacteria of the digestive tract of fish tilapia sp1 and sp2 capable in inhibiting Staphylococcus aureus with a the number of 8.75 mm and Shigella sp with a of 7.16 mm.


2020 ◽  
Vol 2 (2) ◽  
pp. p129
Author(s):  
Victor Pwema Kiamfu ◽  
Jeff Nakweti K ◽  
Willy Lusasi Swana ◽  
Santos Kavumbu Mutanda ◽  
Clement Munganga Kilingwa ◽  
...  

Parasitic diseases of fish are very rarely highlighted in comparison with certain parasitic diseases transmitted to humans through animals or plants such as echinococcosis, bilharziasis or fasciolosis. The aim of this study is to provide knowledge on endoparasites of the human digestive tract that also infest the digestive tracts of two species of Cichlidae fish: Oreochromis niloticus Linnaeus, 1758 and Tilapia tholloni Sauvage, 1884 caught in the Congo River (Malebo Pool). Microscopic observations were made on 42 specimens of O. niloticus fish and 42 of T. tholloni. The results obtained showed that the digestive tracts of fish Oreochromis niloticus host 8 species of parasites grouped in 6 genera, 6 families, 4 classes and 2 branches. The species belonging to the phylum Nemathelminths branch were more abundant (58.8%) than Plathelminths (41.2%). Species Trichurus trichiura (23.5%), Ascaris lumbricoides (20.6%), Strongyloides stercoralis (14.7%), Schistosoma haematobium and Schistosoma intercarlantum (11.8%) were the most frequently observed. On the other hand, microscopic analyses of the digestive tract of Tilapia tholloni revealed the presence of five species of parasites belonging to 3 branches, 4 classes, 4 families and 4 genera. Nemathelminthes (58.3%) and Plathelminthes (25%) are the most represented phylum than Amoebians (16.7%). Enterobius vermicularis (33.3%), Trichocephalus trichuris (25%), Schistosoma haematobium (20.8%) and Entemoeba hystolica (16.7%) were frequently observed. These results confirm the presence of endoparasites in congolese freshwater fish. This information is important in setting up a strategy to protect fish and consumers against these pathogens.


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