Molecular data confirm the existence of attached crustose tetrasporangial thalli in Phymatolithon calcareum (Melobesioideae, Hapalidiaceae, Rhodophyta) from the Mediterranean Sea

Molecular analysis confirms Laurenciella marilzae (Rhodophyta, Rhodomelaceae) in the Mediterranean Sea, a species often misidentified as Laurencia dendroidea

Botanica Marina ◽

10.1515/bot-2020-0030 ◽

2020 ◽

Vol 63 (6) ◽

pp. 527-535

Author(s):

Donatella Serio ◽

Giovanni Furnari ◽

Yola Metti

Keyword(s):

Mediterranean Sea ◽

Molecular Analysis ◽

Molecular Data ◽

Integrative Approach ◽

Molecular Analyses ◽

The Mediterranean ◽

Laurencia Majuscula

AbstractIt was noted that Mediterranean specimens collected at different stations from around Sicily, Italy and referred to as Laurencia dendroidea (as Laurencia majuscula) were similar to the recently described species Laurenciella marilzae. Presented in this study are the results of an integrative approach using both morphology and molecular data (COI-5P + rbcL) to establish which taxon these specimens should be referred to. Molecular analyses show these specimens belong to Laurenciella, and strongly suggest they are within the species L. marilzae. Morphological examinations of these Mediterranean specimens were also detailed and found to support the conclusion that they belong to L. marilzae.

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Morphological and molecular characterization of the toxic dinoflagellate Ostreopsis cf. ovata (Dinophyta) from Brazil (South Atlantic Ocean)

Revista de Biología Tropical ◽

10.15517/rbt.v65i3.29436 ◽

2017 ◽

Vol 65 (3) ◽

pp. 1022 ◽

Cited By ~ 4

Author(s):

Fernando Gómez ◽

Dajun Qiu ◽

Rubens M. Lopes ◽

Senjie Lin

Keyword(s):

Atlantic Ocean ◽

Mediterranean Sea ◽

Current Knowledge ◽

Molecular Data ◽

Resolving Power ◽

Lsu Rdna ◽

Rrna Gene ◽

Cell Dimensions ◽

Sw Atlantic ◽

The Mediterranean

Ostreopsis cf. ovata is a toxic epiphytic dinoflagellate widely distributed in warm waters that often co-occur with species of the genera Coolia, Fukuyoa, Gambierdiscus and Prorocentrum. We investigated a strain isolated from the coast of Ubatuba, Brazil (South-West Atlantic Ocean) by light and epifluorescence microscopies; we also report molecular data based on the LSU rDNA and ITS markers. Cells were 35-65 µm in the dorso-ventral diameter and 20-40 µm wide. We obtained the sequence of a ~1900 base pair region of the rRNA gene cistron. In the LSU rDNA phylogeny, the sequences under the names O. ovata and O. cf. ovata branched into three clades. The ITS marker showed greater resolving power and the sequences of O. ovata/O. cf. ovata split into five clades. Our ITS sequence branched in a clade with sequences of strains from the Mediterranean Sea, European Atlantic coasts, subtropical NE Atlantic, other sequences from Brazil at Rio de Janeiro, and a few sequences from Japan. The cell dimensions and thecal plate arrangement were under the variability range reported in other ocean regions. Our observations confirm O. cf. ovata as the most commonly recorded species of Ostreopsis in the SW Atlantic Ocean. Ostreopsis cf. ovata co-occurred with Coolia malayensis in Brazil and Asia, but it has been commonly reported from the Mediterranean Sea, where C. malayensis has not yet been recorded; while Coolia malayensis has been reported from the Caribbean Sea, but not O. ovata. With the current knowledge, it is difficult to understand the factors that determine the biogeography of the tropical epiphytic dinoflagellates.

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Molecular data and updated morphological description of Flabellina rubrolineata (Nudibranchia: Flabellinidae) from the Red and Arabian seas

Ruthenica, Russian Malacological Journal ◽

10.35885/ruthenica.2021.30(4).1 ◽

2020 ◽

Vol 30 (4) ◽

pp. 183-194

Author(s):

Irina A. Ekimova ◽

Tatiana I. Antokhina ◽

Dimitry M. Schepetov

Keyword(s):

Indian Ocean ◽

Mediterranean Sea ◽

Red Sea ◽

Phylogenetic Trees ◽

Molecular Data ◽

Wide Distribution ◽

Morphological Description ◽

West Pacific ◽

West Indian Ocean ◽

The Mediterranean

Flabellina rubrolineata was believed to have a wide distribution range, being reported from the Mediterranean Sea (non-native), the Red Sea, the Indian Ocean and adjacent seas, and the Indo-West Pacific and from Australia to Hawaii. In the present paper, we provide a redescription of Flabellina rubrolineata, based on specimens collected near the type locality of this species in the Red Sea. The morphology of this species was studied using anatomical dissections and scanning electron microscopy. To place this species in the phylogenetic framework and test the identity of other specimens of F. rubrolineata from the Indo-West Pacific we sequenced COI, H3, 16S and 28S gene fragments and obtained phylogenetic trees based on Bayesian and Maximum likelihood inferences. Our morphological and molecular results show a clear separation of F. rubrolineata from the Red Sea from its relatives in the Indo-West Pacific. We suggest that F. rubrolineata is restricted to only the Red Sea, the Arabian Sea and the Mediterranean Sea and to West Indian Ocean, while specimens from other regions belong to a complex of pseudocryptic species.

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Molecular evidence of three species in the Pseudocaranx dentex complex (Carangidae) in Australian waters

Marine and Freshwater Research ◽

10.1071/mf18445 ◽

2020 ◽

Vol 71 (4) ◽

pp. 518 ◽

Cited By ~ 1

Author(s):

Douglas Bearham ◽

Myrto Robert ◽

Jennifer A. Chaplin ◽

Glenn I. Moore ◽

David V. Fairclough ◽

...

Keyword(s):

Mediterranean Sea ◽

Southern Africa ◽

Molecular Data ◽

Morphological Data ◽

Molecular Evidence ◽

Future Studies ◽

Identification Of Species ◽

The Mediterranean ◽

Proper Interpretation ◽

Conservation And Management

The discovery and identification of species is fundamental to the documentation, conservation and management of biodiversity. The taxonomy of the antitropical Pseudocaranx dentex complex (Carangidae) is confused and inconsistently reported. Previous morphological analyses concluded that this complex consisted of three species, namely P. sp. ‘dentex’, P. georgianus and P. dinjerra, in Australian waters. This study used genetic (COI sequence) and morphological data to evaluate the validity of this conclusion. The COI data showed the presence of three discrete lineages within this complex, which appear to correspond to the above-mentioned three species. They also suggested that P. sp. ‘dentex’ is closely related to, and possibly the same species as, P. dentex from southern Africa and the Mediterranean Sea. Also, the extent of morphological and geographical overlap between P. georgianus and P. dinjerra was greater than previously documented which, uncorrected, could lead to identification errors and present challenges for monitoring and management of harvested stocks of these species. By answering important taxonomic questions, our results will facilitate the proper interpretation of the results of past studies and the design of future studies of the P. dentex complex. They have also highlighted the value of molecular data for identifying species in morphologically conservative taxa.

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Molecular data suggest a hybrid origin for the invasive Caulerpa racemosa (Caulerpales, Chlorophyta) in the Mediterranean Sea

Journal of Evolutionary Biology ◽

10.1046/j.1420-9101.2002.00370.x ◽

2002 ◽

Vol 15 (1) ◽

pp. 122-133 ◽

Cited By ~ 37

Author(s):

C. Durand ◽

M. Manuel ◽

C. F. Boudouresque ◽

A. Meinesz ◽

M. Verlaque ◽

...

Keyword(s):

Mediterranean Sea ◽

Molecular Data ◽

Hybrid Origin ◽

Caulerpa Racemosa ◽

The Mediterranean

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Does GenBank provide a reliable DNA barcode reference to identify small alien oysters invading the Mediterranean Sea?

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315414001027 ◽

2014 ◽

Vol 95 (1) ◽

pp. 111-122 ◽

Cited By ~ 12

Author(s):

F. Crocetta ◽

P. Mariottini ◽

D. Salvi ◽

M. Oliverio

Keyword(s):

Mediterranean Sea ◽

Native Species ◽

Dna Barcode ◽

Genetic Data ◽

Molecular Data ◽

The Public ◽

Variable Species ◽

The Mediterranean ◽

Species Specific

The Mediterranean Sea is currently under siege by a conspicuous alien pressure, and, within some families (e.g. the Ostreidae), the number of native species seems to be remarkably outnumbered by that of the alien ones. We wanted to test the reliability of the molecular data currently available on the small alien oysters recently invading the Mediterranean Sea. Samples from Greece and Turkey, encompassing the known species-specific morphological variation, were sequenced for the markers with the widest taxonomic coverage in the group of small oysters (i.e. the 16S rDNA and the COI). The sequences obtained have been compared with those available in GenBank, and a possible identification at the species level has been finally tested in a DNA-barcoding fashion. The present results clearly demonstrated that our samples belong to a single, morphologically highly variable species. Their 16S sequences were closely related to a sequence registered under the name Dendostrea folium, with a genetic distance which does not warrant conspecificity. Additionally, a remarkable number of sequences retrieved from the GenBank (of both genes) did not form a monophyletic group according to the published classification of the vouchers, suggesting—at least in part—an origin from specimens not properly identified. Both genes seem promising for use as DNA-barcode, although the COI will probably prove more effective. Therefore, we urge the availability of a baseline of oyster pedigreed DNA barcode sequences in the public databases, to allow the use of such genetic data to reliably monitor bio-invasions in the Mediterranean Sea.

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The genus Acanthochitona (Mollusca: Polylacophora) in the Mediterranean Sea: morphological and molecular data

Scientia Marina ◽

10.3989/scimar.2011.75n1171 ◽

2011 ◽

Vol 75 (1) ◽

pp. 171-180 ◽

Cited By ~ 4

Author(s):

Bruno Sabelli ◽

Antonio Bonfitto ◽

Bruno Dell’Angelo ◽

Francesca Evangelisti

Keyword(s):

Mediterranean Sea ◽

Molecular Data ◽

The Mediterranean ◽

Morphological And Molecular Data

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First record of Marphysa chirigota (Annelida: Eunicidae) in the Mediterranean Sea (Gulf of Tunis)

Mediterranean Marine Science ◽

10.12681/mms.25248 ◽

2021 ◽

Author(s):

MARWA CHAIBI ◽

CHIARA ROMANO ◽

ATF AZZOUNA ◽

DANIEL MARTIN

Keyword(s):

16S Rrna ◽

Mediterranean Sea ◽

First Record ◽

Molecular Data ◽

Western Mediterranean ◽

Tunisian Coast ◽

Gulf Of Tunis ◽

Soft Bottoms ◽

The Mediterranean ◽

Marphysa Sanguinea

To date, the genus Marphysa is represented by only three species, Marphysa sanguinea, Marphysa aegypti and Marphysa birgeri in the Mediterranean Sea. Combining morphological, molecular data (16S rRNA and cytochrome c oxidase subunit I mitochondrial loci) and environmental information, we are here presenting the first Mediterranean report of Marphysa chirigota, based on the specimens collected at Radès Station (Gulf of Tunis, western Mediterranean). The current information on the distribution of of the Marphysa species strongly supports that M. sanguinea inhabits hard bottoms and has a restricted distribution close to its type location (south English coast and nearby NE European Atlantic). The specimens from Radès Station, as well as all those reported as M. sanguinea along the Tunisian coast, were found in the shallow water soft bottoms. Therefore, we suggest that the presence of M. sanguinea in Tunisia seems is doubtful, and all Marphysa species reports from Tunisia might correspond to M. chirigota.

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