Molecular analysis confirms Laurenciella marilzae (Rhodophyta, Rhodomelaceae) in the Mediterranean Sea, a species often misidentified as Laurencia dendroidea

2020 ◽  
Vol 63 (6) ◽  
pp. 527-535
Author(s):  
Donatella Serio ◽  
Giovanni Furnari ◽  
Yola Metti
Keyword(s):  
Mediterranean Sea ◽  
Molecular Analysis ◽  
Molecular Data ◽  
Integrative Approach ◽  
Molecular Analyses ◽  
The Mediterranean ◽  
Laurencia Majuscula

AbstractIt was noted that Mediterranean specimens collected at different stations from around Sicily, Italy and referred to as Laurencia dendroidea (as Laurencia majuscula) were similar to the recently described species Laurenciella marilzae. Presented in this study are the results of an integrative approach using both morphology and molecular data (COI-5P + rbcL) to establish which taxon these specimens should be referred to. Molecular analyses show these specimens belong to Laurenciella, and strongly suggest they are within the species L. marilzae. Morphological examinations of these Mediterranean specimens were also detailed and found to support the conclusion that they belong to L. marilzae.

Cyrea Szymborska Gen. Et Sp. Nov., a New Textulariid Foraminifer from the Mediterranean Sea

2018 ◽  
Vol 48 (2) ◽  
pp. 156-163
Author(s):  
Maria Holzmann ◽  
Sylvain Rigaud ◽  
Shahrouz Amini ◽  
Ivan Voltski ◽  
Jan Pawlowski
Keyword(s):  
Mediterranean Sea ◽  
New Genus ◽  
Wall Structure ◽  
Lsu Rdna ◽  
Molecular Analyses ◽  
Rdna Sequences ◽  
Paraphyletic Group ◽  
The Mediterranean ◽  
Genus One ◽  
A New Species

Abstract Textulariid foraminifera are characterized by a multichambered test with an agglutinated wall structure. Recent molecular work has shown that the order Textulariida appears as a paraphyletic group within the class Globothalamea. While larger members of the textulariids are fairly well known, smaller forms like the ones described here are often overlooked or synonymized with known species. A new species and genus was isolated from algal samples collected on the French coast of the Mediterranean Sea. Cyrea szymborska gen. and sp. nov., has a trochospiral chamber arrangement, measuring 100–210 µm in diameter. The organic wall is brownish, opaque, and sparsely agglutinated with diatom frustules and mineral grains, mostly 20 µm or smaller in size. The proloculus is not agglutinated. The extra-umbilical aperture forms a low arch. It is smooth, not agglutinated, and located on the last chamber. The biochemistry of the organic wall was investigated using Raman spectroscopy, showing that it is made of a meshwork of proteoglycans. A combined analysis of SSU and LSU rDNA sequences confirms the position of Cyrea among textulariids, distantly from all other genera. Molecular analyses revealed two phylotypes belonging to the new genus, one of which is formally described here.

Multi-instar descriptions of cave dwelling Erythraeidae (Trombidiformes: Parasitengona) employing an integrative approach

Zootaxa ◽  
2019 ◽  
Vol 4717 (1) ◽  
pp. 137-184 ◽  
Author(s):  
SAMUEL GEREMIAS DOS SANTOS COSTA ◽  
HANS KLOMPEN ◽  
LEOPOLDO FERREIRA DE OLIVEIRA BERNARDI ◽  
LUCIANA CARDOSO GONÇALVES ◽  
DANTE BATISTA RIBEIRO ◽  
...  
Keyword(s):  
Life Cycle ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Species Delimitation ◽  
Morphological Changes ◽  
Molecular Data ◽  
Coi Gene ◽  
Integrative Approach ◽  
Larval Instars ◽  
Individual Host

The life cycle of Parasitengona includes major morphological changes precluding an instar association based only on the morphology. This makes rearing and/or molecular data necessary to associate the heteromorphic instars. Most of the described species are known from either post larval instars or larva. Following a previous study on Palearctic Erythraeidae, in the present study the instar association was made through an integrative approach including rearing trials and molecular analysis of the cytochrome oxidase I (COI) gene with the Bayesian Generalized Mixed Yule Coalescent (bGMYC) algorithm for species delimitation. Two new cave dwelling Erythraeidae (Trombidiformes: Parasitengona) species are described Lasioerythraeus jessicae sp. nov. and Leptus sidorchukae sp. nov. including all active instars. Additionally, a complete description of the previously unknown adults of Charletonia rocciai Treat & Flechtmann, 1979 is provided with notes on the larva and deutonymph. We also demonstrate experimentally that Ch. rocciai larvae are not attached to the same individual host during the entire feeding stage. We discuss the presence of troglomorphisms in Le. sidorchukae sp. nov.; and the distribution of the species. 

scholarly journals Morphological and molecular characterization of the toxic dinoflagellate Ostreopsis cf. ovata (Dinophyta) from Brazil (South Atlantic Ocean)

2017 ◽  
Vol 65 (3) ◽  
pp. 1022 ◽  
Author(s):  
Fernando Gómez ◽  
Dajun Qiu ◽  
Rubens M. Lopes ◽  
Senjie Lin
Keyword(s):  
Atlantic Ocean ◽  
Mediterranean Sea ◽  
Current Knowledge ◽  
Molecular Data ◽  
Resolving Power ◽  
Lsu Rdna ◽  
Rrna Gene ◽  
Cell Dimensions ◽  
Sw Atlantic ◽  
The Mediterranean

Ostreopsis cf. ovata is a toxic epiphytic dinoflagellate widely distributed in warm waters that often co-occur with species of the genera Coolia, Fukuyoa, Gambierdiscus and Prorocentrum. We investigated a strain isolated from the coast of Ubatuba, Brazil (South-West Atlantic Ocean) by light and epifluorescence microscopies; we also report molecular data based on the LSU rDNA and ITS markers. Cells were 35-65 µm in the dorso-ventral diameter and 20-40 µm wide. We obtained the sequence of a ~1900 base pair region of the rRNA gene cistron. In the LSU rDNA phylogeny, the sequences under the names O. ovata and O. cf. ovata branched into three clades. The ITS marker showed greater resolving power and the sequences of O. ovata/O. cf. ovata split into five clades. Our ITS sequence branched in a clade with sequences of strains from the Mediterranean Sea, European Atlantic coasts, subtropical NE Atlantic, other sequences from Brazil at Rio de Janeiro, and a few sequences from Japan. The cell dimensions and thecal plate arrangement were under the variability range reported in other ocean regions. Our observations confirm O. cf. ovata as the most commonly recorded species of Ostreopsis in the SW Atlantic Ocean. Ostreopsis cf. ovata co-occurred with Coolia malayensis in Brazil and Asia, but it has been commonly reported from the Mediterranean Sea, where C. malayensis has not yet been recorded; while Coolia malayensis has been reported from the Caribbean Sea, but not O. ovata. With the current knowledge, it is difficult to understand the factors that determine the biogeography of the tropical epiphytic dinoflagellates. 

scholarly journals Molecular data and updated morphological description of Flabellina rubrolineata (Nudibranchia: Flabellinidae) from the Red and Arabian seas

2020 ◽  
Vol 30 (4) ◽  
pp. 183-194
Author(s):  
Irina A. Ekimova ◽  
Tatiana I. Antokhina ◽  
Dimitry M. Schepetov
Keyword(s):  
Indian Ocean ◽  
Mediterranean Sea ◽  
Red Sea ◽  
Phylogenetic Trees ◽  
Molecular Data ◽  
Wide Distribution ◽  
Morphological Description ◽  
West Pacific ◽  
West Indian Ocean ◽  
The Mediterranean

Flabellina rubrolineata was believed to have a wide distribution range, being reported from the Mediterranean Sea (non-native), the Red Sea, the Indian Ocean and adjacent seas, and the Indo-West Pacific and from Australia to Hawaii. In the present paper, we provide a redescription of Flabellina rubrolineata, based on specimens collected near the type locality of this species in the Red Sea. The morphology of this species was studied using anatomical dissections and scanning electron microscopy. To place this species in the phylogenetic framework and test the identity of other specimens of F. rubrolineata from the Indo-West Pacific we sequenced COI, H3, 16S and 28S gene fragments and obtained phylogenetic trees based on Bayesian and Maximum likelihood inferences. Our morphological and molecular results show a clear separation of F. rubrolineata from the Red Sea from its relatives in the Indo-West Pacific. We suggest that F. rubrolineata is restricted to only the Red Sea, the Arabian Sea and the Mediterranean Sea and to West Indian Ocean, while specimens from other regions belong to a complex of pseudocryptic species.

scholarly journals Zebrus pallaoroi sp. nov.: a new species of goby (Actinopterygii: Gobiidae) from the Mediterranean Sea with a dna-based phylogenetic analysis of the Gobius-lineage

2021 ◽  
pp. 1-33
Author(s):  
Marcelo Kovačić ◽  
Radek Šanda ◽  
Katarína Čekovská ◽  
Tereza Soukupová ◽  
Jasna Vukić
Keyword(s):  
New Species ◽  
Mediterranean Sea ◽  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
The Body ◽  
Integrative Approach ◽  
Fish Family ◽  
Posterior Nostril ◽  
The Mediterranean ◽  
A New Species

Abstract The gobies (Gobiidae) are the most diverse fish family in the Mediterranean Sea. Nevertheless, knowledge on their diversity, taxonomy, and phylogenetic relationships is still inadequate. The phylogenetic analyses reveal two genetically highly distinct clades among specimens identified as Zebrus zebrus. A new species, Zebrus pallaoroi sp. nov., is described based on an integrative approach. The neotype of Zebrus zebrus is designated. Genetic data confirm a pronounced level of divergence between Z. pallaoroi and Z. zebrus, with the mean genetic distance on cytochrome b being 18.1% and 1.07% on rhodopsin. Phylogenetic relationships within the Gobius-lineage were estimated on both markers. Morphologically, Z. pallaoroi is distinguished from the only congener Z. zebrus by having a snout longer than its eye, posterior nostril about 4/5–9/10 of the anterior nostril, eye diameter 4.3−4.7 in head length, ventrolateral head ridges transversally connected on the anterior side by a short transversal ridge, anterior membrane midline depth about 2/3 of the spinous ray, head canal pore α diameter about half of the distance between pore ρ and ρ1, suborbital sensory papillae row 5i going downwards to or near the level of row d, the distance between row 5i and row d absent or much smaller than the length of row 5i, and the body with ten to eleven vertical dark brown bands. Zebrus pallaoroi was recorded from the southern Adriatic, northern Ionian, and northern and western Aegean Seas, and is a cryptobenthic fish from very shallow waters.

Molecular evidence of three species in the Pseudocaranx dentex complex (Carangidae) in Australian waters

2020 ◽  
Vol 71 (4) ◽  
pp. 518 ◽  
Author(s):  
Douglas Bearham ◽  
Myrto Robert ◽  
Jennifer A. Chaplin ◽  
Glenn I. Moore ◽  
David V. Fairclough ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Southern Africa ◽  
Molecular Data ◽  
Morphological Data ◽  
Molecular Evidence ◽  
Future Studies ◽  
Identification Of Species ◽  
The Mediterranean ◽  
Proper Interpretation ◽  
Conservation And Management

The discovery and identification of species is fundamental to the documentation, conservation and management of biodiversity. The taxonomy of the antitropical Pseudocaranx dentex complex (Carangidae) is confused and inconsistently reported. Previous morphological analyses concluded that this complex consisted of three species, namely P. sp. ‘dentex’, P. georgianus and P. dinjerra, in Australian waters. This study used genetic (COI sequence) and morphological data to evaluate the validity of this conclusion. The COI data showed the presence of three discrete lineages within this complex, which appear to correspond to the above-mentioned three species. They also suggested that P. sp. ‘dentex’ is closely related to, and possibly the same species as, P. dentex from southern Africa and the Mediterranean Sea. Also, the extent of morphological and geographical overlap between P. georgianus and P. dinjerra was greater than previously documented which, uncorrected, could lead to identification errors and present challenges for monitoring and management of harvested stocks of these species. By answering important taxonomic questions, our results will facilitate the proper interpretation of the results of past studies and the design of future studies of the P. dentex complex. They have also highlighted the value of molecular data for identifying species in morphologically conservative taxa.

Molecular data suggest a hybrid origin for the invasive Caulerpa racemosa (Caulerpales, Chlorophyta) in the Mediterranean Sea

2002 ◽  
Vol 15 (1) ◽  
pp. 122-133 ◽  
Author(s):  
C. Durand ◽  
M. Manuel ◽  
C. F. Boudouresque ◽  
A. Meinesz ◽  
M. Verlaque ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Molecular Data ◽  
Hybrid Origin ◽  
Caulerpa Racemosa ◽  
The Mediterranean

scholarly journals New discoveries in Eastern Mediterranean mesophotic sponge grounds: updated checklist and description of three novel sponge species

2021 ◽  
Author(s):  
TAL IDAN ◽  
SIGAL SHEFER ◽  
TAMAR FELDSTEIN ◽  
MICHA ILAN
Keyword(s):  
Mediterranean Sea ◽  
Molecular Analysis ◽  
Novel Species ◽  
Eastern Mediterranean ◽  
Baseline Data ◽  
Sponge Species ◽  
Congeneric Species ◽  
The Mediterranean

Rich and diverse mesophotic sponge grounds were recently discovered along the easternmost part of the Mediterranean Sea (Israeli coast). Six sites have been surveyed over the last decade (2009-2019) and the sponge specimens discovered and collected in these expeditions have increased the known sponge richness of the Israeli fauna by ~34%. Here we provide an updated checklist along with the distribution of the various species in seven mesophotic sponge grounds along the coast. Nine mesophotic species have been added, seven of which are new to the Israeli coast. Moreover, we describe three novel species: Hemiasterella verai sp. nov., Axinella venusta sp. nov., and Plakortis mesophotica sp. nov. H. verai sp. nov. differs from other congeneric species in both shape and color; in addition, it has thinner megascleres with more abundant oxeas and microscleres containing both oxyasters and strongylasters. A. venusta sp. nov. is a rather stiff and smooth axinellid with only a few short protruding spicules, and differs from other congeneric species mainly in shape and color. P. mesophotica sp. nov. is characterized by many folds and grooves, and molecular analysis revealed only a 96% similarity with Plakorits simplex.The discovery of these novel species highlights the importance of studying ecosystems that are not readily accessible. Further research of these mesophotic sponge grounds is needed in order to establish the community baseline data and support conservation attempts for these unique habitats.

Does GenBank provide a reliable DNA barcode reference to identify small alien oysters invading the Mediterranean Sea?

2014 ◽  
Vol 95 (1) ◽  
pp. 111-122 ◽  
Author(s):  
F. Crocetta ◽  
P. Mariottini ◽  
D. Salvi ◽  
M. Oliverio
Keyword(s):  
Mediterranean Sea ◽  
Native Species ◽  
Dna Barcode ◽  
Genetic Data ◽  
Molecular Data ◽  
The Public ◽  
Variable Species ◽  
The Mediterranean ◽  
Species Specific

The Mediterranean Sea is currently under siege by a conspicuous alien pressure, and, within some families (e.g. the Ostreidae), the number of native species seems to be remarkably outnumbered by that of the alien ones. We wanted to test the reliability of the molecular data currently available on the small alien oysters recently invading the Mediterranean Sea. Samples from Greece and Turkey, encompassing the known species-specific morphological variation, were sequenced for the markers with the widest taxonomic coverage in the group of small oysters (i.e. the 16S rDNA and the COI). The sequences obtained have been compared with those available in GenBank, and a possible identification at the species level has been finally tested in a DNA-barcoding fashion. The present results clearly demonstrated that our samples belong to a single, morphologically highly variable species. Their 16S sequences were closely related to a sequence registered under the name Dendostrea folium, with a genetic distance which does not warrant conspecificity. Additionally, a remarkable number of sequences retrieved from the GenBank (of both genes) did not form a monophyletic group according to the published classification of the vouchers, suggesting—at least in part—an origin from specimens not properly identified. Both genes seem promising for use as DNA-barcode, although the COI will probably prove more effective. Therefore, we urge the availability of a baseline of oyster pedigreed DNA barcode sequences in the public databases, to allow the use of such genetic data to reliably monitor bio-invasions in the Mediterranean Sea.

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