Evaluation of the protective effects of DNA vaccines encoding an infection-related cysteine protease from Cryptocaryon irritans, the pathogenic organism of cryptocaryoniasis

Aquaculture ◽  
2021 ◽  
pp. 737641
Author(s):  
Yuho Watanabe ◽  
Yuzo Takada ◽  
Maho Kotake ◽  
Kosuke Zenke ◽  
Naoki Itoh ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Dna Vaccines ◽  
Protective Effects ◽  
Pathogenic Organism ◽  
Cryptocaryon Irritans

scholarly journals Legumes Protease Inhibitors as Biopesticides and Their Defense Mechanisms against Biotic Factors

2020 ◽  
Vol 21 (9) ◽  
pp. 3322 ◽  
Author(s):  
Lucio Rodríguez-Sifuentes ◽  
Jolanta Elzbieta Marszalek ◽  
Cristina Chuck-Hernández ◽  
Sergio O. Serna-Saldívar
Keyword(s):  
Protease Inhibitors ◽  
Nutrient Availability ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Defense Mechanisms ◽  
Proteolytic Enzymes ◽  
Protective Effects ◽  
Biotic Factors ◽  
Cysteine Protease Inhibitors

Legumes are affected by biotic factors such as insects, molds, bacteria, and viruses. These plants can produce many different molecules in response to the attack of phytopathogens. Protease inhibitors (PIs) are proteins produced by legumes that inhibit the protease activity of phytopathogens. PIs are known to reduce nutrient availability, which diminishes pathogen growth and can lead to the death of the pathogen. PIs are classified according to the specificity of the mechanistic activity of the proteolytic enzymes, with serine and cysteine protease inhibitors being studied the most. Previous investigations have reported the efficacy of these highly stable proteins against diverse biotic factors and the concomitant protective effects in crops, representing a possible replacement of toxic agrochemicals that harm the environment.

scholarly journals Identification of Polyvalent Vaccine Candidates From Extracellular Secretory Proteins in Vibrio alginolyticus

2021 ◽  
Vol 12 ◽  
Author(s):  
Yu-ming Peng ◽  
Jian-jun Tao ◽  
Su-fang Kuang ◽  
Ming Jiang ◽  
Xuan-xian Peng ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Dna Vaccines ◽  
Vibrio Alginolyticus ◽  
Innate Immune ◽  
Secretory Proteins ◽  
Protective Effects ◽  
Vaccine Candidates ◽  
Polyvalent Vaccine ◽  
Protective Antigens ◽  
Wide Range

Bacterial infections cause huge losses in aquaculture and a wide range of health issues in humans. A vaccine is the most economical, efficient, and environment-friendly agent for protecting hosts against bacterial infections. This study aimed to identify broad, cross-protective antigens from the extracellular secretory proteome of the marine bacterium Vibrio alginolyticus. Of the 69 predicted extracellular secretory proteins in its genome, 16 were randomly selected for gene cloning to construct DNA vaccines, which were used to immunize zebrafish (Danio rerio). The innate immune response genes were also investigated. Among the 16 DNA vaccines, 3 (AT730_21605, AT730_22220, and AT730_22910) were protective against V. alginolyticus infection with 47–66.7% increased survival compared to the control, while other vaccines had lower or no protective effects. Furthermore, AT730_22220, AT730_22910, and AT730_21605 also exhibited cross-immune protective effects against Pseudomonas fluorescens and/or Aeromonas hydrophila infection. Mechanisms for cross-protective ability was explored based on conserved epitopes, innate immune responses, and antibody neutralizing ability. These results indicate that AT730_21605, AT730_22220, and AT730_22910 are potential polyvalent vaccine candidates against bacterial infections. Additionally, our results suggest that the extracellular secretory proteome is an antigen pool that can be used for the identification of cross-protective immunogens.

scholarly journals Drug screening with human SMN2 reporter identifies SMN protein stabilizers to correct SMA pathology

2019 ◽  
Vol 2 (2) ◽  
pp. e201800268 ◽  
Author(s):  
Yiran Wang ◽  
Chongchong Xu ◽  
Lin Ma ◽  
Yongchao Mou ◽  
Bowen Zhang ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Motor Neurons ◽  
Cysteine Protease ◽  
Muscular Atrophy ◽  
Survival Motor Neuron ◽  
Cysteine Protease Inhibitor ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Pass Through ◽  
Smn Protein

Spinal muscular atrophy (SMA), the leading genetic cause of infant mortality, is caused by reduced levels of functional survival motor neuron (SMN) protein. To identify therapeutic agents for SMA, we established a versatile SMN2-GFP reporter line by targeting the human SMN2 gene. We then screened a compound library and identified Z-FA-FMK as a potent candidate. Z-FA-FMK, a cysteine protease inhibitor, increased functional SMN through inhibiting the protease-mediated degradation of both full-length and exon 7–deleted forms of SMN. Further studies reveal that CAPN1, CAPN7, CTSB, and CTSL mediate the degradation of SMN proteins, providing novel targets for SMA. Notably, Z-FA-FMK mitigated mitochondriopathy and neuropathy in SMA patient–derived motor neurons and showed protective effects in SMA animal model after intracerebroventricular injection. E64d, another cysteine protease inhibitor which can pass through the blood–brain barrier, showed even more potent therapeutic effects after subcutaneous delivery to SMA mice. Taken together, we have successfully established a human SMN2 reporter for future drug discovery and identified the potential therapeutic value of cysteine protease inhibitors in treating SMA via stabilizing SMN proteins.

Immunohistochemical localization of lysosomal cysteine protease cathepsins B and L in monkey hippocampal neurons after transient ischemia

1999 ◽  
Vol 19 (3) ◽  
pp. 302-310
Author(s):  
Yukihiko Kohda ◽  
Katsuhiro Tsuchiya ◽  
Junkoh Yamashita ◽  
Masaki Yoshida ◽  
Takashi Ueno ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Hippocampal Neurons ◽  
Immunohistochemical Localization ◽  
Transient Ischemia ◽  
Lysosomal Cysteine Protease

Melatonin reduces high levels of lipid peroxidation induced by potassium iodate in porcine thyroid

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Paulina Iwan ◽  
Jan Stepniak ◽  
Malgorzata Karbownik-Lewinska
Keyword(s):  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Membrane Lipids ◽  
Chemical Properties ◽  
Iodine Concentration ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Protective Effects ◽  
Potassium Iodate ◽  
Hormone Synthesis

Abstract. Iodine is essential for thyroid hormone synthesis. Under normal iodine supply, calculated physiological iodine concentration in the thyroid is approx. 9 mM. Either potassium iodide (KI) or potassium iodate (KIO3) are used in iodine prophylaxis. KI is confirmed as absolutely safe. KIO3 possesses chemical properties suggesting its potential toxicity. Melatonin (N-acetyl-5-methoxytryptamine) is an effective antioxidant and free radical scavenger. Study aims: to evaluate potential protective effects of melatonin against oxidative damage to membrane lipids (lipid peroxidation, LPO) induced by KI or KIO3 in porcine thyroid. Homogenates of twenty four (24) thyroids were incubated in presence of either KI or KIO3 without/with melatonin (5 mM). As melatonin was not effective against KI-induced LPO, in the next step only KIO3 was used. Homogenates were incubated in presence of KIO3 (200; 100; 50; 25; 20; 15; 10; 7.5; 5.0; 2.5; 1.25 mM) without/with melatonin or 17ß-estradiol. Five experiments were performed with different concentrations of melatonin (5.0; 2.5; 1.25; 1.0; 0.625 mM) and one with 17ß-estradiol (1.0 mM). Malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA) concentration (LPO index) was measured spectrophotometrically. KIO3 increased LPO with the strongest damaging effect (MDA + 4-HDA level: ≈1.28 nmol/mg protein, p < 0.05) revealed at concentrations of around 15 mM, thus corresponding to physiological iodine concentrations in the thyroid. Melatonin reduced LPO (MDA + 4-HDA levels: from ≈0.97 to ≈0,76 and from ≈0,64 to ≈0,49 nmol/mg protein, p < 0.05) induced by KIO3 at concentrations of 10 mM or 7.5 mM. Conclusion: Melatonin can reduce very strong oxidative damage to membrane lipids caused by KIO3 used in doses resulting in physiological iodine concentrations in the thyroid.

Protective Effects of Self-Forgiveness on Alcohol Abuse Depend on Severity of Shame

2009 ◽  
Author(s):  
Phillip A. Ianni ◽  
Kenneth E. Hart ◽  
Stephen Hibbard ◽  
Michelle Carroll ◽  
Tobi Wilson ◽  
...  
Keyword(s):  
Alcohol Abuse ◽  
Protective Effects ◽  
Self Forgiveness

Protective Effects of Sense of Community and Ethnic Identity among Urban Adolescents from Diverse Ethnocultural Groups

2009 ◽  
Author(s):  
Pauline Garcia-Reid ◽  
Christina Hamme Peterson ◽  
Robert James Reid ◽  
Paul W. Speer ◽  
N. Andrew Peterson
Keyword(s):  
Ethnic Identity ◽  
Sense Of Community ◽  
Urban Adolescents ◽  
Protective Effects ◽  
Ethnocultural Groups
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