Long-term donors versus non-donor men: Iron metabolism and the atherosclerotic process

2018 ◽  
Vol 272 ◽  
pp. 14-20 ◽  
Author(s):  
Peter Riško ◽  
Jan Pláteník ◽  
Richard Buchal ◽  
Jana Potočková ◽  
Pavel J. Kraml
Keyword(s):  
Iron Metabolism ◽  
Atherosclerotic Process

Iron Localization and Infectious Disease in Chronic Kidney Disease Patients

2016 ◽  
Vol 43 (4) ◽  
pp. 237-244 ◽  
Author(s):  
Takeshi Nakanishi ◽  
Takahiro Kuragano ◽  
Masayoshi Nanami ◽  
Yukiko Hasuike
Keyword(s):  
Infectious Disease ◽  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Iron Metabolism ◽  
Innate Immune ◽  
Intracellular Growth ◽  
Iron Administration ◽  
Lower Infection ◽  
Lower Infection Rate

For patients on dialysis, infection is the second leading cause of mortality. Iron metabolism should be considered in the pathogenesis of infectious disease, as high local iron concentrations favor the growth of many microbes. This review is intended to provide information regarding iron metabolism and infection in chronic kidney disease (CKD) patients. There are 2 reasons these patients may be vulnerable to infection: (1) the excessive iron administered to treat renal anemia could be associated with impairments of the host's innate immune response, (2) CKD-associated inflammation could cause dysregulated iron metabolism. Pathogenic microorganisms can be categorized as extracellular or intracellular pathogens. The proliferation site may determine the degree of virulence. In cases of mainly extracellular microbial growth, the host's strategy of sequestering iron in cells may efficiently inhibit proliferation. However, the same strategy may favor the intracellular growth of microorganisms. The administration of excessive amounts of iron may modify iron localization by an increase in the hepcidin concentration. We conclude that there is a need for large multicenter randomized controlled trials to evaluate the long-term safety of different iron administration patterns that allow for a lower infection rate while still producing efficient erythropoiesis in CKD patients.

Effects of Long-Term Erythropoiesis-Stimulating Agents on Iron Metabolism in Patients on Hemodialysis

2015 ◽  
Vol 19 (6) ◽  
pp. 582-589 ◽  
Author(s):  
Shoko Onuma ◽  
Hirokazu Honda ◽  
Yasuna Kobayashi ◽  
Toshinori Yamamoto ◽  
Tetsuo Michihata ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Erythropoiesis Stimulating Agents

Effects of hypoxia-induced intrauterine growth restriction on cardiac siderosis and oxidative stress

2012 ◽  
Vol 3 (5) ◽  
pp. 350-357 ◽  
Author(s):  
C. F. Rueda-Clausen ◽  
J. S. Morton ◽  
G. Y. Oudit ◽  
Z. Kassiri ◽  
Y. Jiang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Iron Metabolism ◽  
Female Offspring ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Intrauterine Growth ◽  
Adult Rat ◽  
Cardiac Siderosis ◽  
Myocardial Oxidative Stress

We have previously shown that adult rat offspring born intrauterine growth restricted (IUGR) as a result of a prenatal hypoxic insult exhibit several cardiovascular characteristics that are compatible with common manifestations of chronic iron toxicity. As hypoxia is one of the major regulators of iron absorption and metabolism, we hypothesized that hypoxia-induced IUGR offspring will have long-term changes in their ability to regulate iron metabolism leading to myocardial iron deposition and induction of myocardial oxidative stress. Pregnant Sprague Dawley rats were randomized to control (n = 8) or maternal hypoxia (11.5% oxygen; n = 8) during the last 6 days of pregnancy. At birth, litters were reduced to eight pups (four male and four female). At 4 or 12 months of age, offspring were euthanatized and samples (blood and myocardium) were collected. In only the male offspring, IUGR and aging were associated with an increase in myocardial markers of oxidative stress such as oxidized/reduced glutathione ratio and malondialdehyde. Aged male IUGR offspring also exhibited interstitial myocardial remodeling characterized by myocyte loss and disrupted extracellular matrix.Contrary to our hypothesis, however, neither IUGR nor aging were associated with changes in any systemic or local markers of iron metabolism. Our results suggest that hypoxic insults leading to IUGR produce long-term effects on the levels of oxidative stress and connective tissue distribution in the myocardium of male but not female offspring.

scholarly journals The prevalence of risk factors of cardiovascular diseases in persons with arterial hypertension exposed to occupational stress

2017 ◽  
Vol 95 (6) ◽  
pp. 535-544
Author(s):  
A. I. Telegina ◽  
R. A. Liferov ◽  
A. Ya. Fisun ◽  
R. G. Makiev ◽  
V. V. Gornov ◽  
...  
Keyword(s):  
Risk Factors ◽  
Occupational Stress ◽  
Emotional Stress ◽  
Cardiovascular Complications ◽  
Leading Role ◽  
High Prevalence ◽  
Atherosclerotic Process ◽  
High Level

Based on the literature data and the results of their own research, the authors emphasize the importance of studying adverse effects of high emotional load during stress-induced hypertension and draw attention to the high prevalence of modifiable risk factors of cardiovascular disease among servicemen exposed to occupational stress. It has been shown that lifestyle of hypertensive subjects under heavy stress is characterized by irrational changes in eating behavior, high prevalence of smoking, increased alcohol consumption, and low physical activity. The leading role of long-term emotional stress was demonstrated as an independent risk factor of hypertension in servicemen exposed to long-term occupational psycho-emotional stress. Analysis of the intima-media complex thickness in brachiocephalic arteries, depending on the level of psychosocial stress demonstrated that the group of the examined servicemen with hypertension showed changes that might be due to the development of atherosclerotic process, the response to increased flow, and arterial wall tension at a high level of stress. These changes are unidirectional regardless of the duration of hypertension history. Results of evaluation of the overall risk of developing cardiovascular complications based on the SCORE scale in the servicemen with established and newly diagnosed hypertension under heavy stress suggest its enhancement in the next 10 years which makes necessary implementation of a system of measures for preventing and correcting pathological conditions caused by stressful loads. Stratification of risk factors is essential for early diagnosis of hypertension and the choice of adequate therapy in subjects undergoing high psycho-emotional stress.

scholarly journals Associations among Erythroferrone and Biomarkers of Erythropoiesis and Iron Metabolism, and Treatment with Long-Term Erythropoiesis-Stimulating Agents in Patients on Hemodialysis

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0151601 ◽  
Author(s):  
Hirokazu Honda ◽  
Yasuna Kobayashi ◽  
Shoko Onuma ◽  
Keigo Shibagaki ◽  
Toshitaka Yuza ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Erythropoiesis Stimulating Agents

Shunt coronarography in patients with recurrent stenocardia in long term after aorto-coronary bypass

Vestnik ◽  
2021 ◽  
pp. 97-101
Author(s):  
Ж.Б. Турлыгазы ◽  
Д.Ж. Байдиллаева ◽  
Р.А. Бакриев ◽  
А.Б. Канатаева ◽  
А.Г. Шымырбай ◽  
...  
Keyword(s):  
Surgical Intervention ◽  
Coronary Bypass ◽  
First Year ◽  
Dense Adhesion ◽  
Endothelial Layer ◽  
Pericardial Cavity ◽  
Shunt Occlusion ◽  
Shunt Dysfunction ◽  
Atherosclerotic Process

Проблема профилактики и диагностики стеноза - окклюзирования шунтов после аорто - коронарного шунтирования в отдаленном периоде остается нерешенной. Наиболее часто закрываются шунты в течение первого года, возникновение окклюзии венозных шунтов в течение первого года после операции наблюдаются у 25-30% больных, в дальнейшем в течении 5-7 лет частота окклюзии составляет около 2% в год, после этого срока 5% в год. Артериальные шунты остаются проходимы до 98%, и в основном причиной их дисфункции является прогрессирование атеросклеротического процесса и технические погрешности. Основными причинами которые могут привести к нарушению функции шунта в отдаленном периоде считают [1, 2, 4, 6, 8] следующие: 1-техническое (повреждение эндотелиального слоя и стенки аутовенозного трансплантата при его взятии (ретроспективный анализ), чрезмерная длина и перегиб шунта (на шунтографии), натяжение шунта из-за недостаточной его длины, неправильный выбор места наложения дистального анастомоза) [11,12,13]. 2- анатомические факторы[3, 5, 7] . 3 - общие факторы (низкая объемная скорость кровотока по шунту, нестабильность общей гемодинамики, массивные сращения в полости перикарда, гиперкоагуляция, гнойный медиастинит, длительное лихорадочное состояние и неадекватный прием антикоагулянтов. 4 - прогрессирование атеросклероза [9]. 5- использование венозных трансплантантов как одна из важных причин стеноза - окклюзии шунта [10]. The Problem of stenosis prevention and diagnostics - occlusion of shunts after aorto-coronary bypass in long term remains unaddressed. Typically, shunts are closed within the first year, emergence of phleboid shunts occlusion within the first year after surgical intervention is observed in 25-30% of patients, and further frequency of occlusion within 5-7 years is about 2% per year, 5% per year after this term. Arterial shunts is passable up to 98%, and mainly the reason for their dysfunction is the atherosclerotic process progression and technical faults. The main reasons which can results in shunt dysfunction in long term are the following [1, 2, 4, 6, 8]: 1-technical (damage of endothelial layer and paries of autovenous transplant during its drawing (retrospective analysis), excess length and shunt bend (at the shuntography), shunt tension because of its insufficient length, improper location of distal anastomosis application) [11,12,13]. 2- anatomical factors [3, 5, 7] . 3 - general factors (low volumetric blood flow along the shunt, instability of general hemodynamics, dense adhesion in pericardial cavity, hypercoagulability, purulent mediastinitis, prolonged febrile state and inadequate intake of anticoagulants. 4 - atherosclerosis prgression [9]. 5- using venous transplants as one of the important reasons of stenosis - shunt occlusion [10].

Cellular Iron Metabolism Studies Demonstate Safety of Magnetic Tracking of Mesenchymal Stem Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4320-4320
Author(s):  
Edyta Pawelczyk ◽  
Ali S. Arbab ◽  
Sunil Pundit ◽  
Joseph A. Frank
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Light Microscopy ◽  
Iron Metabolism ◽  
Short Term ◽  
Protein Levels ◽  
Cellular Iron ◽  
Magnetic Labeling ◽  
And Storage

Abstract Background: Ferumoxides-protamine sulfate (Fe-Pro) complexes are used for intracellular magnetic labeling of mesenchymal stem cells (MSCs) to non invasively monitor cell trafficking and tissue distribution by in vivo magnetic resonance imaging (MRI). It has been previously shown that there was no short or long- term toxic effects on cell’s viability, proliferation, reactive oxygen species and biological functions including differentiation capacity of MSCs, however the effects of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles labeling on cellular iron metabolism and storage is unknown. Objective: Evaluate the effect of using Fe-Pro complexes for magnetic cell labeling in MSCs on the expression of transferrin receptor (TfR-1) and ferritin, proteins involved in intracellular iron metabolism and storage. Methods: Confluent MSCs were labeled with Fe-Pro complexes at the ratio of 100 ug/mL to 6 ug/mL. After labeling, cells were washed and cultured for 2 months. TfR-1 and ferritin expression were evaluated at the gene and protein levels in total cell lysates, on day 1, 3, 7, 14, 28 and 35 days post-labeling, by real-time polymerase chain reaction (RT-PCR) and Western blotting. MSCs were evaluated for the presence of iron by Prussian blue stain and light microscopy. In addition, TfR-1 and ferritin levels were determined for Fe-Pro labeled and unlabeled MSCs, allowed to slowly multiply, as a result of dividing the cells in half when they reached confluence. Results: All cells were Prussian blue positive on light microscopy following labeling with Fe-Pro. Labeling of MSCs grown to confluence resulted in short-term increase of TfR-1 mRNA (day 1 and day 3, p= 0.0056) without changes in TfR-1 protein levels. Fe- Pro labeled MSC demonstrated an increase in ferritin gene expression on day 7 and 14 (p=0.0003) compared to unlabeled cells, while protein levels were higher compared to unlabeled MSC at each time point (p=0.005). However, ferritin protein levels from Fe-Pro labeled MSCs were not significantly increased after 7 passages. Conclusions: Magnetic labeling of cells with FDA approved ferumoxides complexed to protamine sulfate elicited short-term changes in cellular iron metabolism and storage that was dependent on ability to divide. Fe-Pro labeling did not have any long-term implications validating the safety of Fe-Pro labeling technique for MSCs trafficking studies by MRI.

Carotid replacement with Dacron graft in 292 patients

Vascular ◽  
2016 ◽  
Vol 24 (6) ◽  
pp. 580-589 ◽  
Author(s):  
Igor Koncar ◽  
Jelena Z Ribac ◽  
Nikola S Ilic ◽  
Marko Dragas ◽  
Perica Mutavdzic ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Clinical Examination ◽  
Dacron Graft ◽  
Graft Replacement ◽  
Early Results ◽  
Eversion Endarterectomy ◽  
Atherosclerotic Process ◽  
Graft Reconstruction

Introduction In case of highly atherosclerotic carotid process, carotid graft replacement might be a potential solution for successful procedure. Many studies evaluated the results of vein and polytetrafluorethilen (PTFE) graft usage at the carotid bifurcation, while the experience on the Dacron graft due to extensive atherosclerotic process is missing. The aim of our study was to evaluate 30-day and long-term results of the Dacron graft on carotid artery used in patients with extensive atherosclerotic disease. Material and methods This retrospective study analysed early and long-term neurological outcome as well as Dacron graft patency in patients operated with carotid reconstruction. Early results were confirmed by follow-up clinical examination, whereas late results were assessed by follow-up clinical examination as well as duplex sonographic examination at least 1 year after the surgery. As for statistical methods we used descriptive analysis tests, Chi-square test, and logistic regression. Results Carotid graft replacement was performed in 292 patients, before endarterectomy in 155 (53.09%), or after already attempted unsuccessful eversion endarterectomy in 137 (46.91%). Nineteen (6.5%) patients had a stroke due to ipsilateral and contralateral ischaemia or haemorrhagic in 17 (5.8%), 1 (0.3%) and 1 (0.3%) patients, respectively. Significantly higher rate of strokes occurred when the graft reconstruction was used after the failure of endarterectomy (8.5% vs. 3.5%, p = 0.029). Stroke and death rate was 7.19%. Factors that increased risk of early stroke were the length of plaque in the internal carotid artery measured intraoperatively ( p = 0.025) and the surgical tactic to perform graft reconstruction after attempted extensive endarterectomy ( p = 0.029). Conclusion Low number of patients with carotid stenosis has extensive atherosclerotic process longer than 4 cm that might jeopardise eversion endarterectomy. Carotid graft replacement with Dacron graft provide early results that are comparable with other conduits; however, in such patients reconstruction should be selected individually based on surgical experience and anatomical distribution of stenotic disease. Due to high risk of stroke, only symptomatic patients with such extensive atherosclerotic disease should be operated.

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