Native ion mobility-mass spectrometry and related methods in structural biology

2013 ◽  
Vol 1834 (6) ◽  
pp. 1239-1256 ◽  
Author(s):  
A. Konijnenberg ◽  
A. Butterer ◽  
F. Sobott
Keyword(s):  
Mass Spectrometry ◽  
Structural Biology ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry

scholarly journals Structural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility–mass spectrometry

2019 ◽  
Vol 476 (21) ◽  
pp. 3125-3139 ◽  
Author(s):  
Daniel Shiu-Hin Chan ◽  
Jeannine Hess ◽  
Elen Shaw ◽  
Christina Spry ◽  
Robert Starley ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Structural Biology ◽  
Ion Mobility ◽  
Biosynthetic Pathway ◽  
Screening Tool ◽  
Native Mass Spectrometry ◽  
Ion Mobility Mass Spectrometry ◽  
E Coli ◽  
Structural Insights

Abstract CoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility–mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.

scholarly journals EM∩IM: software for relating ion mobility mass spectrometry and electron microscopy data

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 70-75 ◽  
Author(s):  
Matteo T. Degiacomi ◽  
Justin L. P. Benesch
Keyword(s):  
Electron Microscopy ◽  
Mass Spectrometry ◽  
Structural Biology ◽  
Cross Sections ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Density Maps ◽  
Transmission Electron ◽  
Microscopy Data ◽  
Means Of Transmission

EM∩IM enables the calculation of collision cross-sections from electron density maps obtained, for example, by means of transmission electron microscopy. This capability will further aid the integration of ion mobility mass spectrometry with modern structural biology.

ChemInform Abstract: Applications and Future of Ion Mobility Mass Spectrometry in Structural Biology

ChemInform ◽  
2013 ◽  
Vol 44 (39) ◽  
pp. no-no
Author(s):  
Abraham Lopez ◽  
Teresa Tarrago ◽  
Marta Vilaseca ◽  
Ernest Giralt
Keyword(s):  
Mass Spectrometry ◽  
Structural Biology ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry

Applications and future of ion mobility mass spectrometry in structural biology

2013 ◽  
Vol 37 (5) ◽  
pp. 1283 ◽  
Author(s):  
Abraham López ◽  
Teresa Tarragó ◽  
Marta Vilaseca ◽  
Ernest Giralt
Keyword(s):  
Mass Spectrometry ◽  
Structural Biology ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry

scholarly journals Computational Strategies and Challenges for Using Native Ion Mobility Mass Spectrometry in Biophysics and Structural Biology

2020 ◽  
Vol 92 (16) ◽  
pp. 10872-10880 ◽  
Author(s):  
Timothy M. Allison ◽  
Perdita Barran ◽  
Sarah Cianférani ◽  
Matteo T. Degiacomi ◽  
Valérie Gabelica ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Biology ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

Sign in / Sign up

Export Citation Format

Share Document