Proteomic insights into protostane triterpene biosynthesis regulatory mechanism after MeJA treatment in Alisma orientale (Sam.) Juz.

2021 ◽  
pp. 140671
Author(s):  
Tian Rong ◽  
Zhang Chunchun ◽  
Gu Wei ◽  
Gu Yuchen ◽  
Xu Fei ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Meja Treatment ◽  
Alisma Orientale ◽  
Triterpene Biosynthesis

scholarly journals Methyl jasmonate promote protostane triterpenes accumulation by up-regulating the expression of squalene epoxidases in Alisma orientale

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rong Tian ◽  
Wei Gu ◽  
Yuchen Gu ◽  
Chao Geng ◽  
Fei Xu ◽  
...  
Keyword(s):  
Squalene Epoxidase ◽  
Enzyme Assays ◽  
Pharmacological Activities ◽  
E Coli ◽  
Meja Treatment ◽  
Alisma Orientale ◽  
Rate Limiting ◽  
Triterpene Biosynthesis

AbstractProtostane triterpenes, which are found in Alisma orientale, are tetracyclic triterpenes with distinctive pharmacological activities. The natural distribution of protostane triterpenes is limited mainly to members of the botanical family Alismataceae. Squalene epoxidase (SE) is the key rate-limiting enzyme in triterpene biosynthesis. In this study, we report the characterization of two SEs from A. orientale. AoSE1 and AoSE2 were expressed as fusion proteins in E. coli, and the purified proteins were used in functional research. In vitro enzyme assays showed that AoSE1 and AoSE2 catalyze the formation of oxidosqualene from squalene. Immunoassays revealed that the tubers contain the highest levels of AoSE1 and AoSE2. After MeJA induction, which is the main elicitor of triterpene biosynthesis, the contents of 2,3-oxidosqualene and alisol B 23-acetate increased by 1.96- and 2.53-fold, respectively. In addition, the expression of both AoSE proteins was significantly increased at four days after MeJA treatment. The contents of 2,3-oxidosqualene and alisol B 23-acetate were also positively correlated with AoSEs expression at different times after MeJA treatment. These results suggest that AoSE1 and AoSE2 are the key regulatory points in protostane triterpenes biosynthesis, and that MeJA regulates the biosynthesis of these compounds by increasing the expression of AoSE1 and AoSE2.

scholarly journals MicroRNAome Profile of Euphorbia kansui in Response to Methyl Jasmonate

2019 ◽  
Vol 20 (6) ◽  
pp. 1267
Author(s):  
Peng Li ◽  
Zheni Tian ◽  
Qing Zhang ◽  
Yue Zhang ◽  
Meng Wang ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Developmental Stages ◽  
Regulatory Mechanism ◽  
Pairwise Comparison ◽  
Enrichment Analysis ◽  
P Value ◽  
Target Mrnas ◽  
Meja Treatment ◽  
Euphorbia Kansui ◽  
Significant Gene

miRNAs play vital regulatory roles in different plant developmental stages and in plant response to biotic and abiotic stresses. However, information is limited on the miRNA regulatory mechanism to methyl jasmonate (MeJA). In this study, we used the microRNAome profile to illustrate the relevant regulatory mechanisms of Euphorbia kansui in response to methyl jasmonate (MeJA) through Illumina RNA-Seq. As a result, we identified 875 miRNAs corresponding to 11,277 target mRNAs, among them, 168 known miRNA families representing 6019 target mRNAs sequences were obtained. 452 miRNA-mRNA pairs presented an anti-correlationship (Cor < −0.50 and p-value of correlation ≤ 0.05). The miRNA with a fold change ≥ 2 and a p (p-Value) < 0.05 in pairwise comparison were identified as significant differentially expressed miRNAs (DEMs). The DEMs in MeJA treatment of 0, 24, 36 and 48 h were compared by using Short Time Expression Miner (STEM) cluster and 4 significant gene profiles (p-value ≤ 0.02) were identified. Through the kyoto encyclopedia of genes and genomes (KEGG) pathway and gene ontology (GO) enrichment analysis on all miRNA targets, we identified 33 mRNAs in terpenoid biosynthesis, which were regulated by miRNAs under MeJA treatment, so the miRNA maybe involved in the response of E. kansui plant to exogenous MeJA and the results would provide very useful information on illustrating the regulatory mechanism of E. kansui and also provide an overall view of the miRNAs response to MeJA stress of a non-model plant.

The Variant rs145204276 of GAS5 is Associated with the Development and Prognosis of Gastric Cancer

2018 ◽  
Vol 27 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Qianjun Li ◽  
Gang Ma ◽  
Huimin Guo ◽  
Suhua Sun ◽  
Ying Xu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Survival Rate ◽  
Cancer Progression ◽  
Regulatory Mechanism ◽  
Tumor Stage ◽  
Kaplan Meier ◽  
Non Coding Rna ◽  
Early Tumor ◽  
Long Non Coding Rna ◽  
Clinicopathological Variables

Background & Aims: Down-regulation of the growth arrest specific transcript 5 (GAS5) (long non-coding RNA) is associated with cell proliferation of gastric cancer (GC) and a poor prognosis. We aimed to investigate whether the variant rs145204276 of GAS5 is associated with the prognosis of GC in the Chinese population, and to unveil the regulatory mechanism underlying the GAS5 expression in GC tissues.Method: 1,253 GC patients and 1,354 healthy controls were included. The frequency of the genotype del/del and the allele del of rs145204276 were compared between the patients and the controls and between different subgroups of patients classified by clinicopathological variables. The overall survival rate was analyzed according to the Kaplan-Meier method using the log-rank test.Results: The frequency of genotype del/del was significantly lower in patients than in the controls (7.0% vs. 9.1%, p = 0.001). Kaplan-Meier analysis showed that genotype del/del was significantly associated with a higher survival rate (p = 0.01). Patients with late tumor stage were found to have a significantly lower rate of genotype del/del than those with an early tumor stage (4.9% vs. 8.8%, p = 0.01). Patients with UICC III and IV were found to have a significantly lower rate of genotype del/del than those with UICC I and II (5.3% vs. 8.1%, p = 0.02).Conclusion: The variant rs145204276 of GAS5 is associated with the development and prognosis of GC. The allele del of rs145204276 is associated with a remarkably lower incidence of cancer progression and metastasis.

2195-P: Identification of the Regulatory Mechanism of mTORC1 Signaling Activity in Pancreatic Beta Cells in GCN2 Knockout Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2195-P
Author(s):  
MICHIYO KUDO ◽  
SHUN-ICHIRO ASAHARA ◽  
AYUMI KANNO ◽  
YOSHIAKI KIDO
Keyword(s):  
Beta Cells ◽  
Knockout Mice ◽  
Pancreatic Beta Cells ◽  
Regulatory Mechanism ◽  
Mtorc1 Signaling ◽  
Signaling Activity

Organic Consumer Behavior- Special reference to cereal /cereal products in the southern part of Tamilnadu.

Think India ◽  
2019 ◽  
Vol 22 (3) ◽  
pp. 757-767
Author(s):  
J. ELANCHEZHIAN ◽  
Dr. K. KALAICHELVI
Keyword(s):  
Organic Agriculture ◽  
Regulatory Mechanism ◽  
Organic Food ◽  
State Governments ◽  
Consumer Awareness ◽  
Cereal Products ◽  
Food Standards ◽  
Organic Products ◽  
The Usa ◽  
The Government

Consumers’ interest in organic products is increasing globally. As IFOAM 2016 report, only 1.2 % of the land has been utilized in organic agriculture method. The overall organic market has achieved 89.7 billion $ in 2016 in that, & 48.4 a billion in sales accounted for the USA and German alone. Total registered organic producer in the worldwide is 2.7 million in that India is the leading country which has 835,200 organic producers. But many of them are a small farmer, and they had shared 1.49 million hectares only. The Government of India (GOI) and the state governments have taken several steps to improve the regulatory mechanism and frame several schemes to incentivize organic farming. 2017 December, Food Standards and Safety Authority of India (FSSAI) have recognized both the certification systems (NPOP and PGS-India) valid for organic food products. From these steps, GOI has tried to create confidence in the organic products, so that, domestic consumers and export countries can trust Indian organic products. But still, the organic sector in India suffered from some unique characteristic that is the absence of proper branding, package, consumer awareness, purchasing power, and supply chain issues (Agarwal, 2018).

Sign in / Sign up

Export Citation Format

Share Document