Effects of NOS inhibitors on the benzodiazepines-induced memory impairment of mice in the modified elevated plus-maze task

In phytotherapy, the essential oil from the leaves of Alpinia zerumbet ( Alpinia speciosa K. Schum.) (EOAZ) is used for neuropsychiatric symptoms, such as depression, stress and anxiety, and chronic problems that are associated with reproductive hormone imbalances in women. The chemical composition of EOAZ was analyzed by GC/MS, and the EOAZ properties inducing behavioral alterations in mice were examined by behavioral observations (BO) and an elevated plus-maze task (EPM), widely used as a method for assessing anxiolytic-like behaviors. Five major compounds, p-cymene (28.0 ± 5.0%), 1,8-cineole (17.9 ± 4.2%), terpinen-4-ol (11.9 ± 6.3%), limonene (6.3 ± 2.2%), and camphor (5.2 ± 2.1%) were identified by retention indices, mass spectra and comparison with standards. Inhalational administration of EOAZ (8.7 ppm) induced unique jumping behaviors in mice. To further investigate the behavioral regulatory mechanisms of EOAZ, we administered an intraperitoneal injection of either 10 mg/kg 5-HTP or 10 mg/kg fluoxetine prior to the EOAZ inhalations. By 5-HTP or fluoxetine pretreatments, the jumping frequencies were significantly decreased. In EPM, EOAZ (0.087 and 8.7 ppm) obviously showed the anxiolytic-like activity in mice.

Download Full-text

Effects of glycine or (±)-3-amino-l-hydroxy-2-pyrrolidone microinjections along the rostrocaudal axis of the dorsal periaqueductal gray matter on rats' performance in the elevated plus-maze task.

Behavioral Neuroscience ◽

10.1037/0735-7044.113.1.196 ◽

1999 ◽

Vol 113 (1) ◽

pp. 196-203 ◽

Cited By ~ 23

Author(s):

Karina V. Teixeira ◽

Antonio P. Carobrez

Keyword(s):

Gray Matter ◽

Elevated Plus Maze ◽

Periaqueductal Gray ◽

Maze Task ◽

Periaqueductal Gray Matter ◽

Dorsal Periaqueductal Gray ◽

Plus Maze ◽

Rostrocaudal Axis

Download Full-text

Agmatine induces anxiolysis in the elevated plus maze task in adult rats

Behavioural Brain Research ◽

10.1016/s0166-4328(02)00326-1 ◽

2003 ◽

Vol 141 (1) ◽

pp. 19-24 ◽

Cited By ~ 57

Author(s):

Daniel Lavinsky ◽

Nice Sarmento Arteni ◽

Carlos Alexandre Netto

Keyword(s):

Elevated Plus Maze ◽

Maze Task ◽

Adult Rats ◽

Plus Maze

Download Full-text

Behavioral Changes Induced by Angiotensin AT1 Receptors Blockade in the Rat Brain

European Psychiatry ◽

10.1016/s0924-9338(09)71092-2 ◽

2009 ◽

Vol 24 (S1) ◽

pp. 1-1 ◽

Cited By ~ 2

Author(s):

L. Hritcu ◽

W. Bild ◽

A. Ciobica ◽

V. Artenie ◽

I. Haulica

Keyword(s):

Angiotensin Ii ◽

Elevated Plus Maze ◽

Anxiety State ◽

Avoidance Task ◽

Maze Task ◽

Term Memory ◽

Y Maze ◽

Plus Maze ◽

The Brain

Aims:The brain renin-angiotensin system is involved in learning and memory, but the actual role of angiotensin II and its metabolites in this process has been difficult to comprehend. In the present study we assessed the role of the angiotensin AT1 receptors in certain behavioral effects of angiotensin II using their selective antagonist losartan and PD123319, intracerebroventricularly (icv) administrated.Methods:Male Wistar rats were divided into three groups: 1. sham-operated; 2. Losartan; 3. PD123319. All drugs were stereotaxically icv injected. Learning and memory tests began 2 weeks after the operation, and the ability of the rats to acquire the operant task was studied by means of Y-maze task and passive avoidance task, respectively. The anxiety state was measured in elevated plus maze.Results:Losartan and PD123319 significantly impaired spatial memory in Y-maze task, suggesting significant effects on short-term memory. In passive avoidance task, both angiotensin II antagonist, significantly decreased step-through-latency, suggesting significant effects on long-term memory. In elevated plus maze measuring anxiety, both drugs diminished anxiety state.Conclusions:Our results suggest the considerable involvement of the brain ATi angiotensin receptors in the cognition improving effects of angiotensin.

Download Full-text

Effect of Metformin on Doxorubicin-Induced Memory Dysfunction

Brain Sciences ◽

10.3390/brainsci10030152 ◽

2020 ◽

Vol 10 (3) ◽

pp. 152

Author(s):

Ibrahim Alharbi ◽

Hindi Alharbi ◽

Yasser Almogbel ◽

Abdullah Alalwan ◽

Ahmad Alhowail

Keyword(s):

Cognitive Dysfunction ◽

Memory Impairment ◽

Elevated Plus Maze ◽

Male Rats ◽

Memory Impairments ◽

Glucose Levels ◽

Y Maze ◽

Altered Glucose ◽

Plus Maze ◽

With Memory

Doxorubicin (DOX) is widely used to treat many types of cancer; however, it is associated with chemotherapy-related complications such as cognitive dysfunction, known as chemobrain. Chemobrain affects up to 75% of cancer survivors, and there are currently no available therapeutic options. This study aims to examine whether metformin (MET) can protect against the neurotoxicity caused by DOX treatment. Forty male rats were divided into four groups (10 rats/group): control, DOX, DOX + MET, and MET. Rats treated with DOX received five doses of 4 mg/kg DOX weekly (cumulative dose: 20 mg/kg). For the DOX-MET and MET groups, MET (3 mg/mL) was dissolved in drinking water. Behavioral and glucose tests were performed one day after treatment was completed. We found DOX (4 mg/kg/week, 5 weeks) caused learning and memory impairment in the Y-maze, novel object recognition, and elevated plus maze behavioral tests. MET did not rescue these DOX-induced memory impairments. Neither DOX nor MET nor MET + DOX altered glucose levels following the treatment. In summary, DOX treatment is associated with memory impairment in rats, but MET does not rescue this cognitive dysfunction.

Download Full-text

Anxiolytic Effect of Ocimum basilicum Extract in Rats Tested by Elevated Plus-Maze Task

Avicenna Journal of Neuro Psych Physiology ◽

10.17795/ajnpp-31136 ◽

2015 ◽

Vol 2 (2) ◽

Author(s):

Zahra Nemati ◽

Samaneh Oveisi ◽

Alireza Komaki ◽

Siamak Shahidi

Keyword(s):

Elevated Plus Maze ◽

Anxiolytic Effect ◽

Ocimum Basilicum ◽

Maze Task ◽

Plus Maze

Download Full-text

Studying some neuroprotective effects of Calotropis procera extracts against scopolamine- induced neuropschiatric comorbidities in a rodent model of epilepsy

ROMANIAN BIOTECHNOLOGICAL LETTERS ◽

10.25083/rbl/26.6/3114-3119 ◽

2021 ◽

Vol 26 (6) ◽

pp. 3114-3119

Author(s):

PROSPER T. KINDA ◽

SAMSON GUENNE ◽

BASILE TINDANO ◽

NOUFOU OUEDRAOGO ◽

NABÉRÉ OUATTARA ◽

...

Keyword(s):

Elevated Plus Maze ◽

Neuroprotective Effect ◽

Bark Extract ◽

Calotropis Procera ◽

Root Bark ◽

Protective Effects ◽

Behavioral Tests ◽

Maze Task ◽

Y Maze ◽

Plus Maze

Many plants are largely used in alternative medicine of Burkina Faso for neuropsychiatric disorders treatment. However, their neuro-pharmacological properties are less evaluated through scientific studies. The present study aims to evaluate the neuroprotective effect of Calotropis procera leaves and root-bark aqueous extract, focusing on a scopolamine-induced model of epilepsy in rodents. In this study, we evaluated this plant extracts possible protective effects on the central nervous system, through the behavioral tests and the enzymes activity assays. Thus, elevated plus-maze test and Y-maze task were used to evaluate animals behavioral and UV/visible spectrophotometer methods were used to evaluate the enzyme’s activities in brain’s supernatant. Our results are showing no significant protective effects of leaves extract, but it revealed a significant neuroprotective effect of root-bark aqueous extract, as well as in the behavioral tests and the brain’s oxidative enzymes specific activity evaluation. Indeed, anti-amnesic and anxiolytic activities were observed through Y maze task and elevated plus maze tests for the groups of animals receiving root-bark extract (100 mg/kg b.w.). In these test, inhibition of disturbances of Time spent in Open Arms, Spontaneous Alternation, and Transfer Latency induced after scopolamine administration were recorded with animals received root-bark extract. Likewise, the superoxide dismutase and catalase activity disturbance induced by scopolamine were also inhibited in root-bark extract pre-administered group. Thus, our study provides biochemical and neuro-pharmacological data for traditional use of C. procera for neuropsychiatric disorders treatment, including scopolamine-induced epilepsy symptoms (mainly referring to the psychiatric comorbidities of this disorder).

Download Full-text

The effects of the administration of two different doses of manganese on short-term spatial memory and anxiety-like behavior in rats

Archives of Biological Sciences ◽

10.2298/abs1104031h ◽

2011 ◽

Vol 63 (4) ◽

pp. 1031-1036 ◽

Cited By ~ 3

Author(s):

M. Hogas ◽

A. Ciobica ◽

Simona Hogas ◽

Veronica Bild ◽

L. Hritcu

Keyword(s):

Spatial Memory ◽

Wistar Rats ◽

Elevated Plus Maze ◽

Psychomotor Development ◽

Short Term ◽

Maze Task ◽

Manganese Exposure ◽

Y Maze ◽

Plus Maze ◽

Different Doses

Manganese is a very well known neurotoxic agent. It has been mainly linked to impaired motor skills and disturbed psychomotor development. However, very few aspects are known about the cognitive deficits and behavioral consequences of chronic manganese exposure. In this context, we report herein our findings regarding short-term spatial memory, motor and anxiety-like behavior assessments in male Wistar rats exposed for 45 days to two different doses (3 mg/kg b.w., i.p. and 10 mg/kg b.w., i.p.) of manganese. Behavior testing (Y-maze task and elevated plus maze) was performed after 45 days of manganese administration. Chronic manganese exposure in Wistar rats led to behavioral alterations consisting of cognitive deficiencies in the Y-maze task and anxiety/compulsive-like behaviors in the elevated plus maze, but no motor disturbances as tested by the number of arm entries in the Y-maze. Additional work is necessary to understand the longterm effects of different doses and dosing regimens of manganese on cognitive/affective and motor functioning.

Download Full-text