Agmatine induces anxiolysis in the elevated plus maze task in adult rats

Background: Puberty is a developmental transition in which an estrogenic surge occurs, mediating the release of xenoestrogens, like aluminium. Aluminium’s effect on anxiety in rodents at the different developmental stages is inconsistent. Aims: This study aimed at investigating the effect of the metalloestrogenic property of aluminium on anxiety-like behavioral changes in prepubertal and young adult female rats. Objective: Considering this aim, our objective was to evaluate the anxiety-like behavior by the elevated plus maze in prepubertal and young adult female rats with or without acute exposure to aluminium. Methods: To address this property of aluminium, 5mg/Kg body weight (Al-5) and 10 mg/Kg body weight (Al-10) of aluminium was administered intraperitoneally to female rats at two developmental stages, prepubertal (PP; n = 8 for each dose) and young adult (YA; n = 6 for each dose) for two weeks. Post-treatment, three days behavioral assessment of the rats was done employing elevated plus maze. Results: Reduced escape latency was seen in Al-5, Al-10 pre-pubertal rats, and Al-5 young-adult rats on day 3. A significant reduction in open arm time was seen in the Al-5 young-adult rats. Aluminium treatment in the pre-pubertal rats reduced their head dipping and grooming. Reduced sniffing, head dipping, and stretch-attended posture in the treated young-adult female rats showed that they had impaired risk-taking tendency. Conclusion: Differential effect on the anxiety-like behavior in the pre-pubertal and young-adult female rats might be due to the metalloestrogenic property of aluminium, acting differently on the two age groups.

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Distinct ventral and dorsal hippocampus AP5 anxiolytic effects revealed in the elevated plus-maze task in rats

Neurobiology of Learning and Memory ◽

10.1016/j.nlm.2007.04.007 ◽

2007 ◽

Vol 88 (2) ◽

pp. 177-185 ◽

Cited By ~ 38

Author(s):

Luciane Pereira Nascimento Häckl ◽

Antônio Pádua Carobrez

Keyword(s):

Elevated Plus Maze ◽

Dorsal Hippocampus ◽

Maze Task ◽

Plus Maze

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Sensitivity to diazepam after a single session of forced swim stress in weaning Wistar rats

Acta Pharmaceutica ◽

10.2478/acph-2018-0027 ◽

2018 ◽

Vol 68 (3) ◽

pp. 381-388 ◽

Cited By ~ 1

Author(s):

Blandina Bernal-Morales ◽

Gabriel Guillén-Ruiz ◽

Jonathan Cueto-Escobedo ◽

Juan Francisco Rodríguez-Landa ◽

Carlos M. Contreras

Keyword(s):

Wistar Rats ◽

Elevated Plus Maze ◽

Open Field Test ◽

Clinical Applications ◽

Minimum Effective Dose ◽

Single Session ◽

Adult Rats ◽

Stressed Group ◽

Plus Maze ◽

Forced Swim Stress

Abstract The present study investigated the sensitivity to stress and diazepam in weaning (21-day old) Wistar rats. A single 15-min session of forced swimming was used to induce anxiety-like behavior. The group that was forced to swim exhibited an increase in anxiety-like behavior in the elevated plus maze (EPM) and open field test (OFT) compared to the non-stressed group. Diazepam (1 h before the tests) reduced anxiety-like behavior in rats forced to swim compared to the vehicle stressed group. The dose-response curve for diazepam indicated that the 0.5 mg kg−1 dose (1 h before the EPM and OFT) was the minimum effective dose in reducing anxiety-like behavior without altering locomotor activity in weaning rats. These results indicate that weaning rats can develop anxiety-like behavior after a brief, single session of stress, and that rats at this age are seemingly more sensitive to diazepam than adult rats, which may be taken into account for clinical applications.

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Effects of the Essential Oil from Leaves of Alpinia zerumbet on Behavioral Alterations in Mice

Natural Product Communications ◽

10.1177/1934578x0900400128 ◽

2009 ◽

Vol 4 (1) ◽

pp. 1934578X0900400 ◽

Cited By ~ 8

Author(s):

Shio Murakami ◽

Mariko Matsuura ◽

Tadaaki Satou ◽

Shinichiro Hayashi ◽

Kazuo Koike

Keyword(s):

Essential Oil ◽

Mass Spectra ◽

Elevated Plus Maze ◽

Neuropsychiatric Symptoms ◽

Retention Indices ◽

Reproductive Hormone ◽

Behavioral Alterations ◽

Maze Task ◽

Plus Maze ◽

Alpinia Zerumbet

In phytotherapy, the essential oil from the leaves of Alpinia zerumbet ( Alpinia speciosa K. Schum.) (EOAZ) is used for neuropsychiatric symptoms, such as depression, stress and anxiety, and chronic problems that are associated with reproductive hormone imbalances in women. The chemical composition of EOAZ was analyzed by GC/MS, and the EOAZ properties inducing behavioral alterations in mice were examined by behavioral observations (BO) and an elevated plus-maze task (EPM), widely used as a method for assessing anxiolytic-like behaviors. Five major compounds, p-cymene (28.0 ± 5.0%), 1,8-cineole (17.9 ± 4.2%), terpinen-4-ol (11.9 ± 6.3%), limonene (6.3 ± 2.2%), and camphor (5.2 ± 2.1%) were identified by retention indices, mass spectra and comparison with standards. Inhalational administration of EOAZ (8.7 ppm) induced unique jumping behaviors in mice. To further investigate the behavioral regulatory mechanisms of EOAZ, we administered an intraperitoneal injection of either 10 mg/kg 5-HTP or 10 mg/kg fluoxetine prior to the EOAZ inhalations. By 5-HTP or fluoxetine pretreatments, the jumping frequencies were significantly decreased. In EPM, EOAZ (0.087 and 8.7 ppm) obviously showed the anxiolytic-like activity in mice.

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Effects of glycine or (±)-3-amino-l-hydroxy-2-pyrrolidone microinjections along the rostrocaudal axis of the dorsal periaqueductal gray matter on rats' performance in the elevated plus-maze task.

Behavioral Neuroscience ◽

10.1037/0735-7044.113.1.196 ◽

1999 ◽

Vol 113 (1) ◽

pp. 196-203 ◽

Cited By ~ 23

Author(s):

Karina V. Teixeira ◽

Antonio P. Carobrez

Keyword(s):

Gray Matter ◽

Elevated Plus Maze ◽

Periaqueductal Gray ◽

Maze Task ◽

Periaqueductal Gray Matter ◽

Dorsal Periaqueductal Gray ◽

Plus Maze ◽

Rostrocaudal Axis

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Intrahippocampal administration of BDNF in adult rats affects short-term behavioral plasticity in the Morris water maze and performance in the elevated plus-maze

Hippocampus ◽

10.1002/hipo.10220 ◽

2004 ◽

Vol 14 (7) ◽

pp. 802-807 ◽

Cited By ~ 97

Author(s):

Francesca Cirulli ◽

Alessandra Berry ◽

Flavia Chiarotti ◽

Enrico Alleva

Keyword(s):

Morris Water Maze ◽

Water Maze ◽

Behavioral Plasticity ◽

Elevated Plus Maze ◽

Short Term ◽

Adult Rats ◽

Plus Maze ◽

And Performance

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Effects of NOS inhibitors on the benzodiazepines-induced memory impairment of mice in the modified elevated plus-maze task

Behavioural Brain Research ◽

10.1016/j.bbr.2013.01.038 ◽

2013 ◽

Vol 244 ◽

pp. 100-106 ◽

Cited By ~ 11

Author(s):

Jolanta Orzelska ◽

Sylwia Talarek ◽

Joanna Listos ◽

Sylwia Fidecka

Keyword(s):

Memory Impairment ◽

Elevated Plus Maze ◽

Maze Task ◽

Plus Maze ◽

Nos Inhibitors

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Intergenerational Effects of Sevoflurane in Young Adult Rats

Anesthesiology ◽

10.1097/aln.0000000000002920 ◽

2019 ◽

Vol 131 (5) ◽

pp. 1092-1109 ◽

Cited By ~ 6

Author(s):

Ling-Sha Ju ◽

Jiao-Jiao Yang ◽

Ning Xu ◽

Jia Li ◽

Timothy E. Morey ◽

...

Keyword(s):

Adverse Effects ◽

Prepulse Inhibition ◽

Germ Cells ◽

Elevated Plus Maze ◽

Acoustic Startle ◽

Chloride Ion ◽

Male Rats ◽

Sprague Dawley ◽

Adult Rats ◽

Plus Maze

Abstract Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New Background Sevoflurane administered to neonatal rats induces neurobehavioral abnormalities and epigenetic reprogramming of their germ cells; the latter can pass adverse effects of sevoflurane to future offspring. As germ cells are susceptible to reprogramming by environmental factors across the lifespan, the authors hypothesized that sevoflurane administered to adult rats could induce neurobehavioral abnormalities in future offspring, but not in the exposed rats themselves. Methods Sprague-Dawley rats were anesthetized with 2.1% sevoflurane for 3 h every other day between postnatal days 56 and 60. Twenty-five days later, exposed rats and nonexposed controls were mated to produce offspring. Results Adult male but not female offspring of exposed parents of either sex exhibited deficiencies in elevated plus maze (mean ± SD, offspring of both exposed parents vs. offspring of control parents, 35 ± 12 vs. 15 ± 15 s, P < 0.001) and prepulse inhibition of acoustic startle (offspring of both exposed parents vs. offspring of control parents, 46.504 ± 13.448 vs. 25.838 ± 22.866%, P = 0.009), and increased methylation and reduced expression of the potassium ion-chloride ion cotransporter KCC2 gene (Kcc2) in the hypothalamus. Kcc2 was also hypermethylated in sperm and ovary of the exposed rats. Surprisingly, exposed male rats also exhibited long-term abnormalities in functioning of the hypothalamic-pituitary-gonadal and -adrenal axes, reduced expression of hypothalamic and hippocampal Kcc2, and deficiencies in elevated plus maze (sevoflurane vs. control, 40 ± 24 vs. 25 ± 12 s, P = 0.038) and prepulse inhibition of startle (sevoflurane vs. control, 39.905 ± 21.507 vs. 29.193 ± 24.263%, P < 0.050). Conclusions Adult sevoflurane exposure affects brain development in male offspring by epigenetically reprogramming both parental germ cells, while it induces neuroendocrine and behavioral abnormalities only in exposed males. Sex steroids may be required for mediation of the adverse effects of adult sevoflurane in exposed males.

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Behavioral Changes Induced by Angiotensin AT1 Receptors Blockade in the Rat Brain

European Psychiatry ◽

10.1016/s0924-9338(09)71092-2 ◽

2009 ◽

Vol 24 (S1) ◽

pp. 1-1 ◽

Cited By ~ 2

Author(s):

L. Hritcu ◽

W. Bild ◽

A. Ciobica ◽

V. Artenie ◽

I. Haulica

Keyword(s):

Angiotensin Ii ◽

Elevated Plus Maze ◽

Anxiety State ◽

Avoidance Task ◽

Maze Task ◽

Term Memory ◽

Y Maze ◽

Plus Maze ◽

The Brain

Aims:The brain renin-angiotensin system is involved in learning and memory, but the actual role of angiotensin II and its metabolites in this process has been difficult to comprehend. In the present study we assessed the role of the angiotensin AT1 receptors in certain behavioral effects of angiotensin II using their selective antagonist losartan and PD123319, intracerebroventricularly (icv) administrated.Methods:Male Wistar rats were divided into three groups: 1. sham-operated; 2. Losartan; 3. PD123319. All drugs were stereotaxically icv injected. Learning and memory tests began 2 weeks after the operation, and the ability of the rats to acquire the operant task was studied by means of Y-maze task and passive avoidance task, respectively. The anxiety state was measured in elevated plus maze.Results:Losartan and PD123319 significantly impaired spatial memory in Y-maze task, suggesting significant effects on short-term memory. In passive avoidance task, both angiotensin II antagonist, significantly decreased step-through-latency, suggesting significant effects on long-term memory. In elevated plus maze measuring anxiety, both drugs diminished anxiety state.Conclusions:Our results suggest the considerable involvement of the brain ATi angiotensin receptors in the cognition improving effects of angiotensin.

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