Effects of the Essential Oil from Leaves of Alpinia zerumbet on Behavioral Alterations in Mice

In phytotherapy, the essential oil from the leaves of Alpinia zerumbet ( Alpinia speciosa K. Schum.) (EOAZ) is used for neuropsychiatric symptoms, such as depression, stress and anxiety, and chronic problems that are associated with reproductive hormone imbalances in women. The chemical composition of EOAZ was analyzed by GC/MS, and the EOAZ properties inducing behavioral alterations in mice were examined by behavioral observations (BO) and an elevated plus-maze task (EPM), widely used as a method for assessing anxiolytic-like behaviors. Five major compounds, p-cymene (28.0 ± 5.0%), 1,8-cineole (17.9 ± 4.2%), terpinen-4-ol (11.9 ± 6.3%), limonene (6.3 ± 2.2%), and camphor (5.2 ± 2.1%) were identified by retention indices, mass spectra and comparison with standards. Inhalational administration of EOAZ (8.7 ppm) induced unique jumping behaviors in mice. To further investigate the behavioral regulatory mechanisms of EOAZ, we administered an intraperitoneal injection of either 10 mg/kg 5-HTP or 10 mg/kg fluoxetine prior to the EOAZ inhalations. By 5-HTP or fluoxetine pretreatments, the jumping frequencies were significantly decreased. In EPM, EOAZ (0.087 and 8.7 ppm) obviously showed the anxiolytic-like activity in mice.

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The offspring of alcohol-exposed sires exhibit heightened ethanol intake and behavioral alterations in the elevated plus maze

Alcohol ◽

10.1016/j.alcohol.2021.01.009 ◽

2021 ◽

Vol 92 ◽

pp. 65-72

Author(s):

Jennifer Koabel ◽

Meghan McNivens ◽

Paul McKee ◽

Ricardo Pautassi ◽

Kelly Bordner ◽

...

Keyword(s):

Elevated Plus Maze ◽

Ethanol Intake ◽

Behavioral Alterations ◽

Plus Maze

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Adaptogenic activity of Cinnamomum camphora, Eucalyptus globulus, Lavandula stœchas and Rosmarinus officinalis essential oil used in North-African folk medicine

Cellular and Molecular Biology ◽

10.14715/cmb/2021.67.2.12 ◽

2021 ◽

Vol 67 (2) ◽

pp. 83-88

Author(s):

Mahieddine Boumendjel ◽

Abdennour Boucheker ◽

Sandra Feknous ◽

Faiza Taibi ◽

Naouel Rekioua ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Emotional Disorders ◽

Eucalyptus Globulus ◽

Elevated Plus Maze ◽

Rosmarinus Officinalis ◽

Cinnamomum Camphora ◽

Lavandula Stoechas ◽

Plus Maze ◽

Adaptogenic Activity

Depressive anxiety is one of the most emotional disorders in our industrial societies. Many treatments of phobias exist and are based on plant extracts therapies, which play an important role in the amelioration of the behavior. Our study aimed to evaluate the adaptogenic activity of different essential oils provided from local plants: Cinnamomum camphora (Camphora), Eucalyptus globulus (Blue gum), Lavandula stœchas (Topped lavender) and Rosmarinus officinalis (Rosemary) on Wistar rats. The adaptogenic activity was evaluated on the elevated plus-maze. The efficacy of the extract (200 mL/kg) was compared with the standard anxiolytic drug Diazepam® 1 mg. Animals administered by the essential oil of Lavandula stœchas, Cinnamomum camphora, Rosmarinus officinalis and Eucalyptus globulus showed a behavior similar to those treated with Diazepam®. For groups treated with the following essential oils: Rosmarinus officinalis, Lavandula stoechas and Cinnamomum camphora at a dose of 200 mL/kg, we notice an increase in the time spent on the open arms of the elevated plus-maze and a decrease in time spent on the closed arms of the elevated plus-maze, especially for Rosmarinus officinalis, which explains the anxiolytic effect of these plants. We also notice a decrease in the number of entries in closed arms, open arms and the number of passing to the central square. The increase in the number of entries to open arms with Eucalyptus globulus essential oil shows a reduction in anxiety behavior in rodents and this shows that these plants have an inhibitory effect.

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Distinct ventral and dorsal hippocampus AP5 anxiolytic effects revealed in the elevated plus-maze task in rats

Neurobiology of Learning and Memory ◽

10.1016/j.nlm.2007.04.007 ◽

2007 ◽

Vol 88 (2) ◽

pp. 177-185 ◽

Cited By ~ 38

Author(s):

Luciane Pereira Nascimento Häckl ◽

Antônio Pádua Carobrez

Keyword(s):

Elevated Plus Maze ◽

Dorsal Hippocampus ◽

Maze Task ◽

Plus Maze

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Chemical Composition of Essential Oil from Flower of ‘Shanzhizi’ (Gardenia jasminoides Ellis) and Involvement of Serotonergic System in Its Anxiolytic Effect

Molecules ◽

10.3390/molecules25204702 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4702

Author(s):

Nan Zhang ◽

Mu Luo ◽

Lei He ◽

Lei Yao

Keyword(s):

Essential Oil ◽

Elevated Plus Maze ◽

Anxiolytic Effect ◽

Serotonergic System ◽

Control Treatment ◽

Monoamine Neurotransmitters ◽

Neurotransmitter Receptor ◽

Gardenia Jasminoides ◽

Plus Maze ◽

Gardenia Jasminoides Ellis

Gardenia jasminoides Ellis is a famous fragrant flower in China. Previous pharmacological research mainly focuses on its fruit. In this study, the essential oil of the flower of ‘Shanzhizi’, which was a major variety for traditional Chinese medicine use, was extracted by hydro distillation and analyzed by GC-MS. Mouse anxiety models included open field, elevated plus maze (EPM), and light and dark box (LDB), which were used to evaluate its anxiolytic effect via inhalation. The involvement of monoamine system was studied by pretreatment with neurotransmitter receptor antagonists WAY100635, flumazenil and sulpiride. The monoamine neurotransmitters contents in the prefrontal cortex (PFC) and hippocampus after aroma inhalation were also analyzed. The results showed that inhalation of G. jasminoides essential oil could significantly elevated the time and entries into open arms in EPM tests and the time explored in the light chamber in LDB tests with no sedative effect. WAY100635 and sulpiride, but not flumazenil, blocked its anxiolytic effect. Inhalation of G. jasminoides essential oil significantly down-regulated the 5-HIAA/5-HT in the PFC and reduced the 5-HIAA content in hippocampus compared to the control treatment. In conclusion, inhalation of gardenia essential oil showed an anxiolytic effect in mice. Monoamine, especially the serotonergic system, was involved in its anxiolytic effect.

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Role of 5-HT1A Receptor in the Anxiolytic-Relaxant Effects of Bergamot Essential Oil in Rodent

International Journal of Molecular Sciences ◽

10.3390/ijms21072597 ◽

2020 ◽

Vol 21 (7) ◽

pp. 2597 ◽

Cited By ~ 1

Author(s):

Laura Rombolà ◽

Damiana Scuteri ◽

Chizuko Watanabe ◽

Shinobu Sakurada ◽

Kengo Hamamura ◽

...

Keyword(s):

Essential Oil ◽

Elevated Plus Maze ◽

Relaxant Effect ◽

Serotonergic Receptors ◽

Selective Agonist ◽

Plus Maze ◽

Way 100635 ◽

Pharmacological Data ◽

Citrus Bergamia

The essential oil obtained by the fresh fruit of Citrus bergamia Risso et Poiteau is used worldwide in aromatherapy to reduce pain, facilitate sleep induction, and/or minimize the effects of stress-induced anxiety. Preclinical pharmacological data demonstrate that bergamot essential oil (BEO) modulates specific neurotransmissions and shows an anxiolytic-relaxant effect not superimposable to that of the benzodiazepine diazepam, suggesting that neurotransmissions, other than GABAergic, could be involved. Several studies on essential oils indicate a role for serotonergic (5-HT) neurotransmission in anxiety. Interestingly, among serotonergic receptors, the 5-HT1A subtype seems to play a key role in the control of anxiety. Here, we report that modulation of the 5-HT1A receptor by selective agonist ((±)8-OH-DPAT) or antagonist (WAY-100635) may influence some of the anxiolytic-relaxant effects of BEO in Open Field and Elevated Plus Maze tests.

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Effect on Emotional Behavior and Stress by Inhalation of the Essential oil from Chamaecyparis obtusa

Natural Product Communications ◽

10.1177/1934578x1300800428 ◽

2013 ◽

Vol 8 (4) ◽

pp. 1934578X1300800 ◽

Cited By ~ 3

Author(s):

Hikaru Kasuya ◽

Erika Hata ◽

Tadaaki Satou ◽

Masaki Yoshikawa ◽

Shinichiro Hayashi ◽

...

Keyword(s):

Essential Oil ◽

Elevated Plus Maze ◽

Growth Factor Receptor ◽

Glass Container ◽

Chamaecyparis Obtusa ◽

Emotional Behavior ◽

Antibacterial And Antifungal Activity ◽

Plus Maze ◽

Antibacterial And Antifungal ◽

The Brain

Various effects have been reported in the literature for the essential oil from Chamaecyparis obtusa (EOCO), such as antibacterial and antifungal activity. In this study, we examined the effect of EOCO on emotional behavior and stress-induced biomarkers. Male ICR mice, aged 5 weeks at the start of each experiment, were individually housed in cages for 1 week. After placing each mouse in a glass container and exposing it to EOCO for 90 min, we then investigated the influence on emotional behavior using the elevated-plus maze (EPM) test, which is one of the evaluation methods for anxiolytic-like behavior. Significant anxiolytic-like effects were observed for the 7.0 mg/L air EOCO ( P<0.05). After the EPM test, mice were dissected and changes in the stress-induced biomarkers within the brain were investigated by examining the amounts of fast nerve growth factor receptor (NGFR) and activity regulated cytoskeletal-associated protein (Arc) gene expression, and brain-derived neurotrophic factor (BDNF) and galactokinase 1 (GLK1) protein expression. Significant increases were observed in the amount of NGFR after inhalation of 7.0 mg/L air EOCO ( P<0.05). These results indicate that EOCO has both anxiolytic-like and stress mitigation effects.

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Effects of glycine or (±)-3-amino-l-hydroxy-2-pyrrolidone microinjections along the rostrocaudal axis of the dorsal periaqueductal gray matter on rats' performance in the elevated plus-maze task.

Behavioral Neuroscience ◽

10.1037/0735-7044.113.1.196 ◽

1999 ◽

Vol 113 (1) ◽

pp. 196-203 ◽

Cited By ~ 23

Author(s):

Karina V. Teixeira ◽

Antonio P. Carobrez

Keyword(s):

Gray Matter ◽

Elevated Plus Maze ◽

Periaqueductal Gray ◽

Maze Task ◽

Periaqueductal Gray Matter ◽

Dorsal Periaqueductal Gray ◽

Plus Maze ◽

Rostrocaudal Axis

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Effects of essential oil from Chamaecyparis obtusa on cytokine genes in the hippocampus of maternal separation rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2013-0224 ◽

2014 ◽

Vol 92 (2) ◽

pp. 95-101 ◽

Cited By ~ 10

Author(s):

Hae Jeong Park ◽

Su Kang Kim ◽

Won Sub Kang ◽

Jong-Min Woo ◽

Jong Woo Kim

Keyword(s):

Essential Oil ◽

Life Stress ◽

Early Life ◽

Maternal Separation ◽

Elevated Plus Maze ◽

Early Life Stress ◽

Chamaecyparis Obtusa ◽

Gene Expressions ◽

Cytokine Genes ◽

Plus Maze

We investigated the effects of an essential oil from Chamaecyparis obtusa (EOCO) on early life stress, using maternal separation (MS) rats and a microarray method to analyze the changes in gene expressions caused by EOCO in the hippocampus of MS rats. Rats in the MS groups were separated from their respective mothers from postnatal day (pnd) 14 to 28. Rats in the EOCO-treated groups were exposed to EOCO for 1 or 2 h by inhalation from pnd 21 to 28. The EOCO-treated MS rats showed decreased anxiety-related behaviors compared with the untreated MS rats in the elevated plus-maze (EPM) test. In the microarray analysis, we found that EOCO downregulated the expressions of cytokine genes such as Ccl2, Il6, Cxcl10, Ccl19, and Il1rl in the hippocampus of MS rats, and also confirmed that using reverse transcriptase – PCR. In particular, the expressions of Ccl2 and Il6 were predominantly decreased by EOCO in the hippocampus of MS rats. Interestingly, protein expression was also reduced by EOCO in MS rats. These results indicate that EOCO decreases MS-induced anxiety-related behaviors, and modulates cytokines, particularly Ccl2 and Il6, in the hippocampus of MS rats.

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