Influence of tannase from Serratia marcescens strain IMBL5 on enhancing antioxidant properties of green tea

2020 ◽  
Vol 27 ◽  
pp. 101675 ◽  
Author(s):  
Berchmans Thiyonila ◽  
Mani Kannan ◽  
Naveenagandhi Paulin Reneeta ◽  
Thangaraj Ramya ◽  
Nagarajan Kayalvizhi ◽  
...  
Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2178
Author(s):  
Ana Margarida Teixeira ◽  
Clara Sousa

Medicinal plants have been used since antiquity to cure illnesses and injuries. In the last few decades, natural compounds extracted from plants have garnered the attention of scientists and the Camellia species are no exception. Several species and cultivars are widespread in Asia, namely in China, Japan, Vietnam and India, being also identified in western countries like Portugal. Tea and oil are the most valuable and appreciated Camellia subproducts extracted from Camellia sinensis and Camellia oleifera, respectively. The economic impact of these species has boosted the search for additional information about the Camellia genus. Many studies can be found in the literature reporting the health benefits of several Camellia species, namely C. sinensis, C. oleifera and Camellia japonica. These species have been highlighted as possessing antimicrobial (antibacterial, antifungal, antiviral) and antitumoral activity and as being a huge source of polyphenols such as the catechins. Particularly, epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG), and specially epigallocatechin-3-gallate (EGCG), the major polyphenols of green tea. This paper presents a detailed review of Camellia species’ antioxidant properties and biological activity.


2018 ◽  
Vol 5 (2) ◽  
pp. 60
Author(s):  
Analuddin Analuddin ◽  
Andi Septiana ◽  
Wa Ode Harlis

ABSTRAKPenelitian ini bertujuan untuk menentukan senyawa antioksidan teh hijau dan menjelaskan efektifitas teh hijau daun mangrove sebagai anticholesterol pada mencit. Senyawa kimia bahan antioksidaan teh hijau pada daun mangrove Bruguiera gymnorrhiza, B. parviflora, Rhizophora stylosa, R. mucronata, Lumnitzera racemosa, Ceriops tagal dan C. decandra dianalisis dengan GCMS, sedangkan khasiat teh hijau daun mangroves sebagai antikolesterol di ujikan pada mencit. Hasil penelitian menunjukan bahwa senyawa bahan teh hijau bervariasi diantara daun mangrove yaitu polifenol sederhana ditemukan pada daun semua jenis mangrove yang menjadi sampel penelitian dengan konsentrasi yang bervariasi. Senyawa katekin hanya ditemukan pada daun mangrove Ceriops decandra, L. racemosa, R. mucronata dan R. apiculata. Di sisi lain, flavonoid terdeteksi hanya pada daun C. tagal, B. gymnorrhiza dan R. stylosa, sedangkan senyawa T-flavin hanya ditemukan pada daun B. parviflora. Teh hijau daun mangrove mampu menurunkan kadar kolesterol mencit dengan kisaran 33,33 sampai 53,67% mengindikasikan besarnya potensi daun mangrove sebagai bahan  teh hijau antikolesterol.Kata kunci: bahan teh hijau, polifenol sederhana, katekin, flavonoid, antikolesterol, daun mangroveABSTRACTThis study aimed to determine the antioxidant properties of green tea material in mangrove leaves and elucidate their capacity on reducing the cholesterol of mice. The chemical properties in  leaves of Bruguiera gymnorrhiza, B. parviflora, Rhizophora stylosa, R. mucronata, Lumnitzera racemosa, Ceriops tagal and C. decandra were analyzed by GCMS, while their capability as anticholesterol of mice were examined. The results showed that simple polyphenols were found in all sampled mangrove leaves with different concentration, while the chatechine was found only in leaves of four mangroves including Ceriops decandra, L. racemosa, R. mucronata and R. apiculata. On the other hand, flavonoids was detected only in leaves of C. tagal,  B. gymnorrhiza and R. stylosa. Meanwhile, T-flavine was detected only in leaves of B. parviflora. However, green tea material for all of sampled mangroves showed high capacity to reduce cholesterol of mice that ranging from 33.33% to 53.67%, which indicated high potentitiality of mangroves leaves as green tea material of anticholesterol.Keywords: Green tea material, simple polyphenol, cathechin, flavonoid, anticholesterol, Mangroves leaves


2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Kittipot Sirichaiwetchakoon ◽  
Gordon Matthew Lowe ◽  
Griangsak Eumkeb

Tea is one of the most popular beverages in the world. Camellia sinensis tea (CST) or green tea is widely regarded as a potent antioxidant. In Thailand, Pluchea indica (L.) Less. tea (PIT) has been commercially available as a health-promoting drink. This study focused on free radical scavenging activities of PIT, and its ability to protect isolated human low-density lipoproteins (LDL) from oxidation by chemical agents. A preliminary study to investigate the antioxidant nature of PIT was undertaken. These included common antioxidant assays involving 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), hypochlorous acid (HOCl), and its potential to scavenge peroxynitrite. In separated experiments, isolated human LDL was challenged with either 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), copper (Cu2+), or 3-Morpholinosydnonimine hydrochloride (SIN-1) to induce LDL oxidation. PIT exhibited antioxidant activity in all test systems and performed significantly better than CST in both DPPH (P<0.05; IC50PIT=245.85±15.83 and CST=315.41±24.18 μg/ml) and peroxynitrite scavenging assays. PIT at 75 μg/ml almost fully prevented the peroxynitrite over a 5 h period. Moreover, it displayed similar properties to CST during the antioxidation of isolated human LDL using AAPH, Cu2+, SIN-1, and hypochlorous acid scavenging assays. However, it revealed a significantly lower ABTS scavenging activity than CST (P<0.05; IC50PIT=30.47±2.20 and CST=21.59±0.67 μg/ml). The main constituents of the PIT were identified using LC-MS/MS. It contained 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), and 4,5-O-dicaffeoylquinic acid (4,5-CQ). In conclusion, caffeoyl derivatives in PIT could play an important role in potent antioxidant properties. So, it may be further developed to be antioxidant beverages for preventing atherosclerosis and cardiovascular diseases associated with oxidative stress.


2012 ◽  
Vol 92 (11) ◽  
pp. 2244-2249 ◽  
Author(s):  
Anna Pȩkal ◽  
Paulina Dróżdż ◽  
Magdalena Biesaga ◽  
Krystyna Pyrzynska

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 327 ◽  
Author(s):  
Frederico V. R. Castro ◽  
Mariana A. Andrade ◽  
Ana Sanches Silva ◽  
Maria Fátima Vaz ◽  
Fernanda Vilarinho

Active packaging is becoming progressively more significant as a response to the dynamic changes in current consumer demand and market tendencies. Active packaging is projected to interact directly with the packaged food or with the headspace within the package with the aim of maintaining or extending product quality and shelf-life. Aiming for sustainability, the potential application as biodegradable films of whey protein concentrate (WPC) was evaluated. Aromatic plant’s extracts present high antioxidant properties, representing an alternative for synthetic food additives. The main objective of this study was to verify the effectiveness of an edible WPC film incorporated with a plant-based extract on retarding the lipid oxidation of fresh salmon. Green tea extract (GTE) was chosen to be incorporated into the active film. Fresh salmon was packaged with the control film (WPC) and with active film (WPC–GTE). The oxidation level of non-packaged samples and packaged samples were tested for different storage times. Four methods were applied to evaluate lipid oxidation state of fresh salmon: peroxide value, p-anisidine value, thiobarbituric acid reactive substances (TBARS) assay, and monitoring of hexanal. The results obtained in this study indicate that the whey protein active film was successfully produced, and it was effective in delaying lipid oxidation of fresh salmon samples until the 14th day of storage.


2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Yadav KC ◽  
Ashok Parajuli ◽  
Bishnu Bahadur Khatri ◽  
Lila Devi Shiwakoti

Tea is a popular drink with refreshing and functional properties. Bud, 1st leaf, and 2nd leaf of five varieties of tea clones (Gumti Takda-78, Ambari, Chiniya, and Tinali, which are popular in tea plantation area of Nepal) were collected and used for preparing green and orthodox black tea to study antioxidant activity, phytochemicals profile, chemical content, and sensory parameters. One or two leaves were hand-plucked from each bush to get a sample of about 100 leaves and processed for green and orthodox black tea for different clones of tea plants. Phytochemicals, antioxidant activity, and caffeine content were found higher in bud followed by 1st leaf and 2nd leaf for all clones of tea plants. Both types of tea (green and black) from Gumti were significantly (p<0.05) higher having tannin content, flavonoid content, total polyphenol content, caffeine content, and IC50 value of 49.15 and 36.23 (mg GAE/g dry extract), 358.9 and 350.4 (mg QE/g dry extract), 590.5 and 570 (mg GAE/g dry extract), 2.85 and 2.94%, and 45.15 & 51.88 μg/mL, respectively. Green and orthodox tea from Takda-78 was found higher in caffeine content and the least in Tinali for both types of tea. Moisture, water extract, total ash, acid-insoluble ash, and crude fiber content in tea (green and black) from Gumti were found to be 5.4% & 5.37%, 65.89% & 71.46%, 5.524% & 6.52%, 0.46% & 0.57%, and 7.96% & 10.27%, respectively. The ratio of theaflavin and thearubigin (TF : TR) was found 1 : 8.61, 1 : 9.36, 1 : 9.70, 1 : 12.87, and 1 : 6.36 in Takda-78, Ambari, Gumti, Chiniya, and Tinali respectively. The total quality score in green tea (85.13%) and black tea (85.78%) from Gumti was significantly higher than others. Phytochemicals and antioxidant properties of green tea were significantly (p<0.05) higher than those of orthodox black tea for all clones of tea plant. This study suggests Gumti variety to be used in green and orthodox black tea processing for higher phytochemical, chemical, sensory quality, and antioxidant activity.


Author(s):  
SHENG-DUN LIN ◽  
CHIH-HUNG LIANG ◽  
EN-HUI LIU ◽  
JENG-LEUN MAU

2009 ◽  
Vol 89 (6) ◽  
pp. 627-485 ◽  
Author(s):  
Masood Dehkharghanian ◽  
Stéphane Salmieri ◽  
Monique Lacroix ◽  
Mookambeswaran A. Vijayalakshmi

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