Modeled structure-based computational redesign of a glycosyltransferase for the synthesis of rebaudioside D from rebaudioside A

2020 ◽  
Vol 159 ◽  
pp. 107626
Author(s):  
Min Lin ◽  
Fei Wang ◽  
Yushan Zhu
Keyword(s):  
Rebaudioside A ◽  
Modeled Structure ◽  
Rebaudioside D

scholarly journals Correction to RQ3, A Natural Rebaudioside D Isomer, Was Obtained from Glucosylation of Rebaudioside A Catalyzed by the CGTase Toruzyme 3.0 L

2020 ◽  
Vol 68 (42) ◽  
pp. 11875-11876
Author(s):  
Qingbin Guo ◽  
Tongtong Zhang ◽  
Nifei Wang ◽  
Yongmei Xia ◽  
Zhuoyu Zhou ◽  
...  
Keyword(s):  
Rebaudioside A ◽  
Rebaudioside D

scholarly journals Effect of different drying methods on the composition of steviol glycosides in Stevia rebaudiana Bertoni leaves

2017 ◽  
Vol 31 (1) ◽  
pp. 139-144
Author(s):  
Irma Aranda-González ◽  
David Betancur-Ancona ◽  
Luis Chel-Guerrero ◽  
Yolanda Moguel-Ordóñez
Keyword(s):  
Low Cost ◽  
Stevia Rebaudiana ◽  
Hplc Method ◽  
Drying Methods ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Drying Method ◽  
Sun Drying ◽  
Stevia Rebaudiana Bertoni ◽  
Rebaudioside D

Abstract Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g−1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers.

RQ3, A Natural Rebaudioside D Isomer, Was Obtained from Glucosylation of Rebaudioside A Catalyzed by the CGTase Toruzyme 3.0 L

2019 ◽  
Vol 67 (28) ◽  
pp. 8020-8028 ◽  
Author(s):  
Qingbin Guo ◽  
Tongtong Zhang ◽  
Nifei Wang ◽  
Yongmei Xia ◽  
Zhuoyu Zhou ◽  
...  
Keyword(s):  
Rebaudioside A ◽  
Rebaudioside D

scholarly journals Sweetness profiles of glycosylated rebaudioside A and its binary mixtures with allulose and maltitol

2021 ◽  
Author(s):  
Jinsil Jung ◽  
SooHyun Kim ◽  
Sunghee Park ◽  
Jae-Hee Hong
Keyword(s):  
Binary Mixtures ◽  
Bitter Taste ◽  
Descriptive Analysis ◽  
Sensory Profile ◽  
Rebaudioside A ◽  
Relative Sweetness ◽  
Alternative Sweetener ◽  
Rebaudioside D ◽  
Sensory Profiles ◽  
Choice Method

AbstractRebaudioside A is a promising natural alternative sweetener but they produce increased bitterness, astringency, and unpleasant aftertastes. Glycosylation and blending with different sweeteners are known to improve the sensory characteristics of rebaudioside A. The present study was conducted to identify the relative sweetness and sensory profile of glycosyl rebaudioside A (g-reb A). The relative sweetness of g-reb A compared to 5% sucrose was determined using the two-alternative forced choice method. The sensory profiles of g-reb A and its mixtures with allulose and maltitol (1:1 ratio) were compared to those of rebaudioside A, rebaudioside D, rebaudioside M, sucralose, allulose, maltitol, and sucrose using descriptive analysis conducted by eight trained panelists. The relative sweetness of g-reb A was 155, which was lower than that of rebaudioside A. In addition, the bitter taste and aftertaste, astringency, and sweet onset of g-reb A were decreased compared to those of rebaudioside A.

scholarly journals Daily Light Integral Influences Steviol Glycoside Biosynthesis and Relative Abundance of Specific Glycosides in Stevia

HortScience ◽  
2015 ◽  
Vol 50 (10) ◽  
pp. 1479-1485 ◽  
Author(s):  
Jennifer M. Evans ◽  
Veronica A. Vallejo ◽  
Randolph M. Beaudry ◽  
Ryan M. Warner
Keyword(s):  
Solar Radiation ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Daily Light Integral ◽  
Steviol Glycoside ◽  
Long Day ◽  
Genetic And Environmental Factors ◽  
Rebaudioside D ◽  
Light Integral

The biosynthesis of steviol glycosides is affected by both genetic and environmental factors. To evaluate the influence of total daily solar radiation or daily light integral (DLI) under long-day conditions on steviol glycoside concentration, we grew Stevia rebaudiana under ambient irradiance or varying levels of shading at different times of the year in both greenhouse and field environments, resulting in DLIs ranging from 3.55 to 20.31 mol·m−2·d−1 in the greenhouse and 10.32 to 39.7 mol·m−2·d−1 in the field. Total steviol glycoside concentration of selected leaves from greenhouse-grown plants increased as DLI increased up to ca. 10 mol·m−2·d−1, remaining constant with further increases in DLI, and was similar across the range of DLIs evaluated in the field. DLI influenced both the concentration and the relative proportions of specific steviol glycosides. Rebaudioside A concentration increased as DLI increased from 3.55 to 8.53 mol·m−2·d−1, remaining similar with further increases in DLI. Rebaudioside D and stevioside concentration of selected leaves from field-grown plants decreased by 22% and 13%, respectively, as DLI increased from 10.32 to 39.7 mol·m−2·d−1, while rebaudioside A and M concentrations remained similar across this DLI range. Collectively, these results indicate that the greatest influence of DLI on steviol glycoside concentration occurs under relatively low DLIs (<10 mol·m−2·d−1). However, higher DLIs can significantly affect the synthesis of minor glycosides of increasing commercial importance including rebaudioside D.

scholarly journals Isolation and Structure Elucidation of Rebaudioside D2 from Bioconversion Reaction of Rebaudioside A to Rebaudioside D

2014 ◽  
Vol 9 (8) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Indra Prakash ◽  
Cynthia Bunders ◽  
Krishna P. Devkota ◽  
Romila D. Charan ◽  
Catherine Ramirez ◽  
...  
Keyword(s):  
Spectral Data ◽  
Structure Elucidation ◽  
Rebaudioside A ◽  
Mass Spectral Data ◽  
Complete Structure ◽  
Mass Spectral ◽  
Steviol Glycoside ◽  
Extensive Analysis ◽  
Rebaudioside D

We report the isolation and complete structure of an isomer of rebaudioside D, known as rebaudioside D2. This novel steviol glycoside was isolated from a bioconversion reaction of rebaudioside A to rebaudioside D. Rebaudioside D2 possesses a relatively rare 1→6 sugar linkage, which was discovered by extensive analysis of NMR (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data.

scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

scholarly journals The Application of Pureed Butter Beans and a Combination of Inulin and Rebaudioside A for the Replacement of Fat and Sucrose in Sponge Cake: Sensory and Physicochemical Analysis

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 254
Author(s):  
Aislinn M. Richardson ◽  
Andrey A. Tyuftin ◽  
Kieran N. Kilcawley ◽  
Eimear Gallagher ◽  
Maurice G. O’Sullivan ◽  
...  
Keyword(s):  
Physicochemical Analysis ◽  
Acceptance Testing ◽  
Rebaudioside A ◽  
Simultaneous Reduction ◽  
Sponge Cake ◽  
Overall Acceptability ◽  
Sensory Acceptance ◽  
Intensity Parameters ◽  
Sensory Parameters ◽  
Instrumental Texture

Determining minimum levels of fat and sucrose needed for the sensory acceptance of sponge cake while increasing the nutritional quality was the main objective of this study. Sponge cakes with 0, 25, 50 and 75% sucrose replacement (SR) using a combination of inulin and Rebaudioside A (Reb A) were prepared. Sensory acceptance testing (SAT) was carried out on samples. Following experimental results, four more samples were prepared where fat was replaced sequentially (0, 25, 50 and 75%) in sucrose-replaced sponge cakes using pureed butter beans (Pbb) as a replacer. Fat-replaced samples were investigated using sensory (hedonic and intensity) and physicochemical analysis. Texture liking and overall acceptability (OA) were the only hedonic sensory parameters significantly affected after a 50% SR in sponge cake (p < 0.05). A 25% SR had no significant impact on any hedonic sensory properties and samples were just as accepted as the control sucrose sample. A 30% SR was chosen for further experiments. After a 50% fat replacement (FR), no significant differences were found between 30% sucrose-replaced sponge cake samples in relation to all sensory (hedonic and intensity) parameters investigated. Flavour and aroma intensity attributes such as buttery and sweet and, subsequently, liking and OA of samples were negatively affected after a 75% FR (p < 0.05). Instrumental texture properties (hardness and chewiness (N)) did not discriminate between samples with increasing levels of FR using Pbb. Moisture content increased significantly with FR (p < 0.05). A simultaneous reduction in fat (42%) and sucrose was achieved (28%) in sponge cake samples without negatively affecting OA. Optimised samples contained significantly more dietary fibre (p < 0.05).

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