scholarly journals Effect of different drying methods on the composition of steviol glycosides in Stevia rebaudiana Bertoni leaves

2017 ◽  
Vol 31 (1) ◽  
pp. 139-144
Author(s):  
Irma Aranda-González ◽  
David Betancur-Ancona ◽  
Luis Chel-Guerrero ◽  
Yolanda Moguel-Ordóñez
Keyword(s):  
Low Cost ◽  
Stevia Rebaudiana ◽  
Hplc Method ◽  
Drying Methods ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Drying Method ◽  
Sun Drying ◽  
Stevia Rebaudiana Bertoni ◽  
Rebaudioside D

Abstract Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g−1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers.

scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

scholarly journals Effect of Algae Treatment On Stevia Rebaudiana Growth

2018 ◽  
Vol 46 (2) ◽  
pp. 73-77
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Blood Pressure ◽  
South America ◽  
Red Light ◽  
Stevia Rebaudiana ◽  
Reduce Blood Pressure ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Biomass Yields ◽  
Set Up

Abstract Stevia rebaudiana Bertoni is a small, perennial and herbaceous shrub which originated in Paraguay (South America). Stevia rebaudiana is not native to Hungary but its cultivation and consumption may have many benefits, e.g. to reduce blood pressure and as a non-caloric sweetener. Steviol glycosides, mostly stevioside and rebaudioside A, located in the leaves are about 200–300 times sweeter than sucrose. S. rebaudina cultivation in Hungary would offer many opportunities in healthcare and the sweet industry. With the aim of achieving good green biomass yields, the effect of MACC4 autotrophic and heterotrophic algae strains was investigated by testing them as both leaf and soil fertilizers in the soil of Stevia rebaudiana seedlings and in its aqueous rooting experiments. In one of the later set up, the formation of roots was improved by combining the application of red light and algae treatment.

scholarly journals METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF STEVIOSIDE, REBAUDIOSIDE-A, REBAUDIOSIDE C AND DULCOSIDE A CONTAINED IN STEVIA REBAUDIANA BERTONI USING HPLC-ELSD

2016 ◽  
Vol 8 (9) ◽  
pp. 1
Author(s):  
Supriyadi . ◽  
Siswandono . ◽  
Mochammad Yuwono
Keyword(s):  
Method Development ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
System Mode ◽  
Development And Validation

<p><strong>Objective</strong><strong>:</strong><strong> </strong>To develop and validate a selective HPLC-ELSD method for determination of steviol glycosides contained in <em>Stevia rebaudiana</em>, mainly stevioside, rebauside A, rebaudioside C, and dulcoside A. <strong></strong></p><p><strong>Methods: </strong>The chromatographic separation of stevioside, rebaudioside A, rebaudioside C, and dulcoside A was achieved using Phenomenex Luna column 250 mm x 4.6 mm i.d. in isocratic system mode with a mobile phase of acetonitrile-water (35: 65). The temperature of nebulization and evaporization of the ELS detector was set at 50 <sup>o</sup>C and 70 <sup>o</sup>C, respectively.<strong></strong></p><p><strong>Results: </strong>The good separation of stevioside, rebaudioside A, rebaudioside C, and dulcoside A was obtained, yielding the resolution of all the analytes more than 1.5. All the validation parameters like specificity, linearity, range, accuracy and precision met the acceptance criteria according to ICH guidelines.<strong></strong></p><p><strong>Conclusion: </strong>The proposed HPLC-ELSD method is simple and sensitive for the simultaneously detection and determination of stevioside, rebaudioside A, rebaudioside C and dulcoside A contained in <em>Stevia rebaudiana</em>. The method was successfully applied for the determination of the samples product of <em>Stevia rebaudiana</em>.</p><p><strong>Keywords: </strong>Stevioside, Rebaudioside A, Rebaudioside C, Dulcoside A, HPLC-ELSD</p>

scholarly journals Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni

2016 ◽  
Vol 8 (4) ◽  
pp. 1953-1958
Author(s):  
Neena Kumari ◽  
R. C. Rana ◽  
Y. P. Sharma ◽  
Suresh Kumar
Keyword(s):  
Growth And Development ◽  
Stevia Rebaudiana ◽  
Vegetative Stage ◽  
Growth Stages ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Flowering Stage ◽  
Plant Parts ◽  
Stevia Rebaudiana Bertoni ◽  
Green Stem

In the present investigation, the dynamics of steviol glycosides (stevioside and rebaudioside-A) of Stevia rebaudiana with their growth stages were studied. The study aimed to examine the best stage of harvesting (month of the year) the crop with respect to maximum accumulation of stevioside and rebaudioside-A content in different plant parts (leaves, green stem and woody stem). The results showed that the maximum stevioside content in leaves (8.55%) was found in June month (vegetative stage). Rebaudioside-A content in leaves (7.00%) was at its peak in August (vegetative stage). Whereas, higher stevioside and rebaudioside-A content was found for green stem (0.93%) and woody stem (0.18%) during September month (flowering stage). Leaves showed maximum yields of stevioside (17.60g) and rebaudioside-A (13.75g) per plant in July month. The study indicated that it is economical to harvest the leaves of S. rebaudiana rather than harvesting whole aerial biomass in vegetative phase (July month).

scholarly journals New Diterpene Glycosides from Stevia rebaudiana Bertoni: Rebaudioside VIII and Rebaudioside IXd

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301
Author(s):  
Gil Ma ◽  
Allison Bechman ◽  
Cynthia Bunders ◽  
Krishna P. Devkota ◽  
Romila D. Charan ◽  
...  
Keyword(s):  
Spectral Data ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Mass Spectral Data ◽  
Complete Structure ◽  
Mass Spectral ◽  
Stevia Rebaudiana Bertoni ◽  
Rebaudioside D

With our interest of new diterpene glycosides with better taste profile than Rebaudioside M, we isolated and characterized two steviol glycosides with three additional glucose attached at the C-13 of Rebaudioside M or Rebaudioside D from an extract of Stevia rebaudiana Bertoni. Here we report the complete structure elucidations of diterpene glycosides on the basis of NMR experiments (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY, NOESY) and mass spectral data. Steviol glycosides that possess eight or nine glucose units are rarely reported in literature and could have an impact on the natural sweetener catalog.

scholarly journals Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

2017 ◽  
Vol 9 (4) ◽  
pp. 2114-2126 ◽  
Author(s):  
Neena Kumari ◽  
Suresh Kumar
Keyword(s):  
Quantitative Estimation ◽  
Stevia Rebaudiana ◽  
Analytical Techniques ◽  
Extraction Methods ◽  
Estimation Methods ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
The World ◽  
Low Calorie Sweetener

The Stevia genus encompasses about 200 herbs and shrubs species. Stevia rebaudiana, one of the members has gained commercial importance as a natural low calorie sweetener, due to the presence of high con-centration of stevioside and rebaudioside - A (25% to 45% of stevioside content) in the leaves. The major processes involved in the production and quantification of steviol glycosides are extraction, purification and estimation. Various extraction methods have been used for extraction of steviol glycosides in the world. The extraction methods of steviol glycosides mostly differed at the stage of clarification of extracts. The present study is an attempt to summarize the scattered literature and reports on a single podium. Moreover, it also depicts up to date literature regarding numerous extraction, purification and quantitative estimation methods for steviol glycosides

scholarly journals Investigations into Enzymatic Bioconversion to Form Rebaudioside A from Stevioside

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Experimental Design ◽  
Statistical Design ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Statistical Experimental Design ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Perennial Shrub ◽  
Enzymatic Bioconversion ◽  
Full Factorial

Stevia rebaudiana Bertoni is a perennial shrub from South America that produces steviol glycosides which are 200-300 times sweeter than sugar. Stevioside and rebaudioside A are the main sweetening components of its leaves. Steviol glycosides are diterpenoids whose biosynthetic pathways have four steps in common with gibberellic acid formation. The most important enzyme in the biosynthetic pathway expressed by the gene UGT76G1 is referred to as UDP-glycosyltransferase 76G1. It converts stevioside into rebaudioside A. The former has a bitter aftertaste and is a poorer sweetener but is most abundant. This enzyme can be produced in a next generation recombinant way by Escherichia coli and Saccharomyces cerevisiae. Trichoderma longibrachiatum produces the enzyme β-1,3-glucanase enzyme, which can perform a transglycosylation between stevioside to gain rebaudioside A. In our study, a full-factorial statistical experimental design that applies different glycosyl donors, temperatures, enzyme-to-substrate ratios and pH's as factors in order to achieve higher Reb A ratios in S. rebaudiana extracts after transglycosylation is reported. The presented statistical design was appropriate to indicate relevant and significant factors, providing a good basis for an upcoming experimental design of a real-world optimization.

scholarly journals Continuation of Reversed-Phase HPLC Analysis Studies of Steviol Glycosides Isolated From Stevia rebaudiana Bertoni

2015 ◽  
Vol 4 (2) ◽  
pp. 87 ◽  
Author(s):  
Srinivasarao Meneni ◽  
Venkata Sai Prakash Chaturvedula
Keyword(s):  
High Performance ◽  
Food Additives ◽  
Stevia Rebaudiana ◽  
Reversed Phase ◽  
Hplc Method ◽  
Ideal Condition ◽  
Steviol Glycosides ◽  
Expert Committee ◽  
Stevia Rebaudiana Bertoni ◽  
Different Temperatures

<p>Additional High Performance Liquid Chromatography (HPLC) studies were performed on the nine sweet steviol glycosides reported in Joint Expert Committee on Food Additives (JECFA) namely rebaudioside A, steviolbioside, stevioside, rubusoside, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside F, and dulcoside A isolated from the leaves of <em>Stevia rebaudiana</em>. Using Reversed-Phase (RP) HPLC method, individual retention times and area for nine naturally occurring <em>ent</em>-kaurane diterpene glycosides of <em>S. rebaudiana</em> have been determined at five different temperatures: 40, 45, 50, 55, and 60 ºC at three different pH 2.4, 2.6, and 2.8. HPLC results suggested that temperatures 50 and 55 ºC at pH 2.4 would be ideal condition for better separation of steviol glycosides.</p>

scholarly journals Stevia rebaudiana Bertoni: The Interaction of Night Interruption on Gas Exchange, Flowering Delay, and Steviol Glycosides Synthesis

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 543
Author(s):  
Jesús Antonio Rivera-Avilez ◽  
Alfredo Jarma-Orozco ◽  
Marcelo F. Pompelli
Keyword(s):  
Leaf Area ◽  
Vegetative Growth ◽  
Stevia Rebaudiana ◽  
Net Photosynthesis ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Beverage Industry ◽  
Food And Beverage Industry ◽  
Food And Beverage

The Stevia market is estimated to be USD 1.14 billion in 2028 due to its acceptance in the food and beverage industry. Stevia rebaudiana and its two more relevant edulcorants: stevioside (St) and rebaudioside A (Reb-A) can reach 450-fold sweeter than sucrose. The species is considered a long night plant, promoting flowering and shortening vegetative growth. Thus, to increase the leaf area and St and Reb-A increase, we broke the long night with a short light pulse, here called night interruption (NI). In this study, three NI times and two S. rebaudiana genotypes were tested to promote larger vegetative growth, flowering delay, and higher synthesis of steviol glycosides (SvGly). The main goal of this study was to demonstrate that NI increased net photosynthesis (9% to 20%), the internode length (59%), the leaf area (25%), while delays in 4 to 10 days of the flowering phase, impacting in 17% to 25% more St and Reb-A, respectively. Here we describe an inexpensive flowering delay, elongation of vegetative growth, allowing extended harvesting, which could yield four to five annual harvesting of leaves, increasing the production in 21% to 24% more St and Reb-A yield (kg ha−1).

scholarly journals β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

2021 ◽  
Vol 9 ◽  
Author(s):  
Anastasia Zerva ◽  
Koar Chorozian ◽  
Anastasia S. Kritikou ◽  
Nikolaos S. Thomaidis ◽  
Evangelos Topakas
Keyword(s):  
Low Cost ◽  
Stevia Rebaudiana ◽  
Cost Effective ◽  
Sweet Taste ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Glycosyl Hydrolases ◽  
Maximum Conversion ◽  
Acid Whey ◽  
Industrial Byproducts

Stevia rebaudiana Bertoni is a plant cultivated worldwide due to its use as a sweetener. The sweet taste of stevia is attributed to its numerous steviol glycosides, however, their use is still limited, due to their bitter aftertaste. The transglycosylation of steviol glycosides, aiming at the improvement of their taste, has been reported for many enzymes, however, glycosyl hydrolases are not extensively studied in this respect. In the present study, a β-glucosidase, MtBgl3a, and a β-galactosidase, TtbGal1, have been applied in the transglycosylation of two steviol glycosides, stevioside and rebaudioside A. The maximum conversion yields were 34.6 and 33.1% for stevioside, while 25.6 and 37.6% were obtained for rebaudioside A conversion by MtBgl3a and TtbGal1, respectively. Low-cost industrial byproducts were employed as sugar donors, such as cellulose hydrolyzate and acid whey for TtbGal1- and MtBgl3a- mediated bioconversion, respectively. LC-HRMS analysis identified the formation of mono- and di- glycosylated products from stevioside and rebaudioside A. Overall, the results of the present work indicate that both biocatalysts can be exploited for the design of a cost-effective process for the modification of steviol glycosides.

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