scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

scholarly journals Effect of different drying methods on the composition of steviol glycosides in Stevia rebaudiana Bertoni leaves

2017 ◽  
Vol 31 (1) ◽  
pp. 139-144
Author(s):  
Irma Aranda-González ◽  
David Betancur-Ancona ◽  
Luis Chel-Guerrero ◽  
Yolanda Moguel-Ordóñez
Keyword(s):  
Low Cost ◽  
Stevia Rebaudiana ◽  
Hplc Method ◽  
Drying Methods ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Drying Method ◽  
Sun Drying ◽  
Stevia Rebaudiana Bertoni ◽  
Rebaudioside D

Abstract Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g−1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers.

scholarly journals Effect of Algae Treatment On Stevia Rebaudiana Growth

2018 ◽  
Vol 46 (2) ◽  
pp. 73-77
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Blood Pressure ◽  
South America ◽  
Red Light ◽  
Stevia Rebaudiana ◽  
Reduce Blood Pressure ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Biomass Yields ◽  
Set Up

Abstract Stevia rebaudiana Bertoni is a small, perennial and herbaceous shrub which originated in Paraguay (South America). Stevia rebaudiana is not native to Hungary but its cultivation and consumption may have many benefits, e.g. to reduce blood pressure and as a non-caloric sweetener. Steviol glycosides, mostly stevioside and rebaudioside A, located in the leaves are about 200–300 times sweeter than sucrose. S. rebaudina cultivation in Hungary would offer many opportunities in healthcare and the sweet industry. With the aim of achieving good green biomass yields, the effect of MACC4 autotrophic and heterotrophic algae strains was investigated by testing them as both leaf and soil fertilizers in the soil of Stevia rebaudiana seedlings and in its aqueous rooting experiments. In one of the later set up, the formation of roots was improved by combining the application of red light and algae treatment.

scholarly journals METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF STEVIOSIDE, REBAUDIOSIDE-A, REBAUDIOSIDE C AND DULCOSIDE A CONTAINED IN STEVIA REBAUDIANA BERTONI USING HPLC-ELSD

2016 ◽  
Vol 8 (9) ◽  
pp. 1
Author(s):  
Supriyadi . ◽  
Siswandono . ◽  
Mochammad Yuwono
Keyword(s):  
Method Development ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
System Mode ◽  
Development And Validation

<p><strong>Objective</strong><strong>:</strong><strong> </strong>To develop and validate a selective HPLC-ELSD method for determination of steviol glycosides contained in <em>Stevia rebaudiana</em>, mainly stevioside, rebauside A, rebaudioside C, and dulcoside A. <strong></strong></p><p><strong>Methods: </strong>The chromatographic separation of stevioside, rebaudioside A, rebaudioside C, and dulcoside A was achieved using Phenomenex Luna column 250 mm x 4.6 mm i.d. in isocratic system mode with a mobile phase of acetonitrile-water (35: 65). The temperature of nebulization and evaporization of the ELS detector was set at 50 <sup>o</sup>C and 70 <sup>o</sup>C, respectively.<strong></strong></p><p><strong>Results: </strong>The good separation of stevioside, rebaudioside A, rebaudioside C, and dulcoside A was obtained, yielding the resolution of all the analytes more than 1.5. All the validation parameters like specificity, linearity, range, accuracy and precision met the acceptance criteria according to ICH guidelines.<strong></strong></p><p><strong>Conclusion: </strong>The proposed HPLC-ELSD method is simple and sensitive for the simultaneously detection and determination of stevioside, rebaudioside A, rebaudioside C and dulcoside A contained in <em>Stevia rebaudiana</em>. The method was successfully applied for the determination of the samples product of <em>Stevia rebaudiana</em>.</p><p><strong>Keywords: </strong>Stevioside, Rebaudioside A, Rebaudioside C, Dulcoside A, HPLC-ELSD</p>

scholarly journals Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni

2016 ◽  
Vol 8 (4) ◽  
pp. 1953-1958
Author(s):  
Neena Kumari ◽  
R. C. Rana ◽  
Y. P. Sharma ◽  
Suresh Kumar
Keyword(s):  
Growth And Development ◽  
Stevia Rebaudiana ◽  
Vegetative Stage ◽  
Growth Stages ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Flowering Stage ◽  
Plant Parts ◽  
Stevia Rebaudiana Bertoni ◽  
Green Stem

In the present investigation, the dynamics of steviol glycosides (stevioside and rebaudioside-A) of Stevia rebaudiana with their growth stages were studied. The study aimed to examine the best stage of harvesting (month of the year) the crop with respect to maximum accumulation of stevioside and rebaudioside-A content in different plant parts (leaves, green stem and woody stem). The results showed that the maximum stevioside content in leaves (8.55%) was found in June month (vegetative stage). Rebaudioside-A content in leaves (7.00%) was at its peak in August (vegetative stage). Whereas, higher stevioside and rebaudioside-A content was found for green stem (0.93%) and woody stem (0.18%) during September month (flowering stage). Leaves showed maximum yields of stevioside (17.60g) and rebaudioside-A (13.75g) per plant in July month. The study indicated that it is economical to harvest the leaves of S. rebaudiana rather than harvesting whole aerial biomass in vegetative phase (July month).

Salt Stress-Induced Changes in In Vitro Cultured Stevia rebaudiana Bertoni: Effect on Metabolite Contents, Antioxidant Capacity and Expression of Steviol Glycosides-Related Biosynthetic Genes

2019 ◽  
Vol 38 (4) ◽  
pp. 1341-1353 ◽  
Author(s):  
Simone Ribeiro Lucho ◽  
Marcelo Nogueira do Amaral ◽  
Priscila Ariane Auler ◽  
Valmor João Bianchi ◽  
María Ángeles Ferrer ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Capacity ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Biosynthetic Genes ◽  
Stevia Rebaudiana Bertoni ◽  
Induced Changes

scholarly journals Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

2017 ◽  
Vol 9 (4) ◽  
pp. 2114-2126 ◽  
Author(s):  
Neena Kumari ◽  
Suresh Kumar
Keyword(s):  
Quantitative Estimation ◽  
Stevia Rebaudiana ◽  
Analytical Techniques ◽  
Extraction Methods ◽  
Estimation Methods ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
The World ◽  
Low Calorie Sweetener

The Stevia genus encompasses about 200 herbs and shrubs species. Stevia rebaudiana, one of the members has gained commercial importance as a natural low calorie sweetener, due to the presence of high con-centration of stevioside and rebaudioside - A (25% to 45% of stevioside content) in the leaves. The major processes involved in the production and quantification of steviol glycosides are extraction, purification and estimation. Various extraction methods have been used for extraction of steviol glycosides in the world. The extraction methods of steviol glycosides mostly differed at the stage of clarification of extracts. The present study is an attempt to summarize the scattered literature and reports on a single podium. Moreover, it also depicts up to date literature regarding numerous extraction, purification and quantitative estimation methods for steviol glycosides

scholarly journals Investigations into Enzymatic Bioconversion to Form Rebaudioside A from Stevioside

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Experimental Design ◽  
Statistical Design ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Statistical Experimental Design ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Perennial Shrub ◽  
Enzymatic Bioconversion ◽  
Full Factorial

Stevia rebaudiana Bertoni is a perennial shrub from South America that produces steviol glycosides which are 200-300 times sweeter than sugar. Stevioside and rebaudioside A are the main sweetening components of its leaves. Steviol glycosides are diterpenoids whose biosynthetic pathways have four steps in common with gibberellic acid formation. The most important enzyme in the biosynthetic pathway expressed by the gene UGT76G1 is referred to as UDP-glycosyltransferase 76G1. It converts stevioside into rebaudioside A. The former has a bitter aftertaste and is a poorer sweetener but is most abundant. This enzyme can be produced in a next generation recombinant way by Escherichia coli and Saccharomyces cerevisiae. Trichoderma longibrachiatum produces the enzyme β-1,3-glucanase enzyme, which can perform a transglycosylation between stevioside to gain rebaudioside A. In our study, a full-factorial statistical experimental design that applies different glycosyl donors, temperatures, enzyme-to-substrate ratios and pH's as factors in order to achieve higher Reb A ratios in S. rebaudiana extracts after transglycosylation is reported. The presented statistical design was appropriate to indicate relevant and significant factors, providing a good basis for an upcoming experimental design of a real-world optimization.

scholarly journals Stevia rebaudiana Bertoni: The Interaction of Night Interruption on Gas Exchange, Flowering Delay, and Steviol Glycosides Synthesis

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 543
Author(s):  
Jesús Antonio Rivera-Avilez ◽  
Alfredo Jarma-Orozco ◽  
Marcelo F. Pompelli
Keyword(s):  
Leaf Area ◽  
Vegetative Growth ◽  
Stevia Rebaudiana ◽  
Net Photosynthesis ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Beverage Industry ◽  
Food And Beverage Industry ◽  
Food And Beverage

The Stevia market is estimated to be USD 1.14 billion in 2028 due to its acceptance in the food and beverage industry. Stevia rebaudiana and its two more relevant edulcorants: stevioside (St) and rebaudioside A (Reb-A) can reach 450-fold sweeter than sucrose. The species is considered a long night plant, promoting flowering and shortening vegetative growth. Thus, to increase the leaf area and St and Reb-A increase, we broke the long night with a short light pulse, here called night interruption (NI). In this study, three NI times and two S. rebaudiana genotypes were tested to promote larger vegetative growth, flowering delay, and higher synthesis of steviol glycosides (SvGly). The main goal of this study was to demonstrate that NI increased net photosynthesis (9% to 20%), the internode length (59%), the leaf area (25%), while delays in 4 to 10 days of the flowering phase, impacting in 17% to 25% more St and Reb-A, respectively. Here we describe an inexpensive flowering delay, elongation of vegetative growth, allowing extended harvesting, which could yield four to five annual harvesting of leaves, increasing the production in 21% to 24% more St and Reb-A yield (kg ha−1).

A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]

2011 ◽  
Vol 91 (1) ◽  
pp. 1-27 ◽  
Author(s):  
Ashok Kumar Yadav ◽  
S. Singh ◽  
D. Dhyani ◽  
P. S. Ahuja
Keyword(s):  
Stevia Rebaudiana ◽  
Leaf Size ◽  
Mutation Breeding ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Efficient System ◽  
Perennial Plant ◽  
Stevia Rebaudiana Bertoni ◽  
Marker Loci ◽  
Additive Gene

Yadav, A. K., Singh, S., Dhyani, D. and Ahuja, P. S. 2011. A review on the improvement of Stevia [Stevia rebaudiana (Bertoni)]. Can. J. Plant Sci. 91: 1–27. Stevia rebaudiana (Bertoni) is a herbaceous perennial plant (2n=22) of genus Stevia Cav., which consists of approximately 230 species of herbaceous, shrub and sub-shrub plants. Leaves of stevia produce diterpene glycosides (stevioside and rebaudiosides), non-nutritive, non-toxic, high-potency sweeteners and may substitute sucrose as well as other synthetic sweetners, being 300 times sweeter than sucrose. In addition to its sweetening property, it has medicinal values and uses. Stevia is self-incompatible plant and the pollination behaviour is entomophilous. Rebaudioside-A is of particular interest among the glycosides produced in the leaves of stevia because of the most desirable flavour profile, while, stevioside is responsible for aftertaste bitterness. Development of new varieties of S. rebaudiana with a higher content of rebaudioside-A and a reduced content of stevioside is the primary aim of plant breeders concerned with the improvement and utilization of this source of natural sweeteners. The proportions of rebaudioside-A and -C are controlled by a single additive gene known to be co-segregating suggesting synthesis by the same enzyme. Stevioside and rebaudioside-A are negatively correlated, while rebaudioside-A and -C are positively correlated. Conventional plant breeding approaches such as selection and intercrossing among various desirable genotypes is the best method for improving quality traits in a highly cross-pollinated crop like stevia. Various plant types with larger amounts of specific glycoside have already been patented, such as RSIT 94-1306, RSIT 94-75, RSIT 95-166-1 through selection and intercrossing. Composites and synthetics can be used to capture part of the available heterosis because of the high degree of natural out-crossing and the absence of an efficient system of pollination control. Synthetics and composites like “AC Black Bird” and “PTA-444” have already been developed. Polyploidy results in better adaptability of individuals and increased organ and cell sizes. Tetraploids have larger leaf size, thickness and have potential use in increasing biomass and yield in comparison with diploid strains. Characters of interest with low variability in the population may be improved through mutation breeding. Use of biotechnological approaches, such as tissue culture for the mass propagation of elite genotypes, anther culture for development of pure homozygous doubled haploid and molecular marker technology for identification of marker loci linked to rebaudioside-A trait, can create new opportunities for plant breeders. Understanding the mechanism and pathway of biosynthesis of steviol glycosides can help to improve the glycoside profile by up-regulation and down-regulation of genes.

scholarly journals Tetra-glucopyranosyl Diterpene ent-Kaur-16-en-19-oic Acid and ent-13(S)-Hydroxyatisenoic Acid Derivatives from a Commercial Extract of Stevia rebaudiana (Bertoni) Bertoni

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3328 ◽  
Author(s):  
Wilmer Perera ◽  
Ion Ghiviriga ◽  
Douglas Rodenburg ◽  
Kamilla Alves ◽  
Frank Wiggers ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
2D Nmr ◽  
The United States ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Chemical Structures ◽  
Stevia Rebaudiana Bertoni ◽  
Commercial Extract ◽  
United States Food ◽  
States Food

Stevia rebaudiana and its diterpene glycosides are one of the main focuses of food companies interested in developing novel zero calorie sugar substitutes since the recognition of steviol glycosides as Generally Recognized as Safe (GRAS) by the United States Food and Drug Administration. Rebaudioside A, one of the major steviol glycosides of the leaves is more than 200 times sweeter than sucrose. However, its lingering aftertaste makes it less attractive as a table-top sweetener, despite its human health benefits. Herein, we report the purification of two novel tetra-glucopyranosyl diterpene glycosides 1 and 3 (rebaudioside A isomers) from a commercial Stevia rebaudiana leaf extract compounds, their saponification products compounds 2 and 4, together with three known compounds isolated in gram quantities. Compound 1 was determined to be 13-[(2-O-β-d-glucopyranosyl-6-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]ent-kaur-16-en-19-oic acid-β-d-glucopyranosy ester (rebaudioside Z), whereas compound 3 was found to be 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]ent-hydroxyatis-16-en-19-oic acid -β-d-glucopyranosy ester. Two new tetracyclic derivatives with no sugar at position C-19 were prepared from rebaudiosides 1 and 3 under mild alkaline hydrolysis to afford compounds 2 13-[(2-O-β-d-glucopyranosyl-6-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]ent-kaur-16-en-19-oic acid (rebaudioside Z1) and 4 13-[(2-O-β-d-glucopyranosyl-3-O-β-d-glucopyranosyl-β-d-glucopyranosyl) oxy]ent-hydroxyatis-16-en-19-oic acid. Three known compounds were purified in gram quantities and identified as rebaudiosides A (5), H (6) and J (7). Chemical structures were unambiguously elucidated using different approaches, namely HRESIMS, HRESI-MS/MS, and 1D-and 2D-NMR spectroscopic data. Additionally, a high-quality crystal of iso-stevioside was grown in methanol and its structure confirmed by X-ray diffraction.

Sign in / Sign up

Export Citation Format

Share Document