Ultrasonic-assisted extraction of polysaccharide from Dendrobium officinale: Kinetics, thermodynamics and optimization

AbstractUltrasonic-assisted extraction (UAE) is increasingly emerging as a highly effective extraction technique. This extraction technique is affected by several experimental factors. The present work aimed to optimize the ultrasonic-assisted extraction of antioxidants from Ulva lactuca (sea lettuce), widespread macroalgae growing along the Mediterranean coast. In this respect, a full-factorial design (23) was employed to assess the effect of three different factors at two levels and their interactions on the extraction of antioxidants from sea lettuce algal biomass. The studied factors were extraction solvent, time of extraction, and temperature. The two levels chosen for extraction solvent were 100% ethanol and 50% ethanol, for the sonication time (1 h and 3 h) and temperature (25 °C and 40 °C). All experiments were done using an ultrasonic bath, and the biomass to solvent ratio was kept at 1:5. Total antioxidant capacity and quercetin concentration were set as the two responses for optimum output. The results showed that the temperature and solvent were the dominating factors that significantly affect the extraction process. The optimum extraction parameters were extraction time of 1 h, 50% ethanol, and temperature of 25 °C. Under these conditions, the maximum value for TAA was calculated as 2166.51 mg of ascorbic acid equivalent per gram (mg/g of AAE), and quercetin showed a maximum calculated value of 42.5 mg/g with combined desirability of 0.91 for the two responses. The present study results indicate that U. lactuca can be used as a source of antioxidants and phenolic compounds that can be applied in food and medicine at optimum extraction conditions. Graphical abstract

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Determination of nonylphenol and nonylphenol ethoxylates in environmental solid samples by ultrasonic-assisted extraction and high performance liquid chromatography-fluorescence detection

Journal of Chromatography A ◽

10.1016/j.chroma.2007.01.101 ◽

2007 ◽

Vol 1146 (2) ◽

pp. 157-163 ◽

Cited By ~ 43

Author(s):

L. Núñez ◽

E. Turiel ◽

J.L. Tadeo

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Fluorescence Detection ◽

High Performance ◽

Nonylphenol Ethoxylates ◽

Solid Samples ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

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Optimization of Ultrasonic-Assisted Extraction, Characterization, and Antioxidant Activities of Polysaccharides From Sojae Semen Praeparatum

Natural Product Communications ◽

10.1177/1934578x211020622 ◽

2021 ◽

Vol 16 (5) ◽

pp. 1934578X2110206

Author(s):

Yongshuai Jing ◽

Ruijuan Zhang ◽

Lan Li ◽

Danshen Zhang ◽

Yu Liu ◽

...

Keyword(s):

Antioxidant Activities ◽

Deae Cellulose ◽

Chemical Compositions ◽

Hydroxyl Groups ◽

Ultrasonic Frequency ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

In this study, response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction parameters of Sojae Semen Praeparatum polysaccharides (SSPP-80), the optimum conditions were determined as follows: ultrasonic frequency of 100 W, ultrasonic power of 80 Hz, ultrasonic temperature of 52℃, ultrasonic time of 23 minutes, and liquid to raw material ratio of 40 mL/g. Based on these conditions, polysaccharides extraction rate was 7.72% ± 0.26%. Then, 2 novel polysaccharides (SSPP-80‐1, SSPP-80‐2) were isolated from SSPP by DEAE-cellulose 52 chromatography. The chemical compositions, physicochemical properties, and structure of SSPPs were investigated by simultaneous thermal analyzer (TGA), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FI-IR), and high-performance liquid chromatography (HPLC). The results showed that SSPP-80 and 2 fractions were mainly composed of mannose (Man), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara). In addition, the antioxidant activities were evaluated against the DPPH and hydroxyl radical in vitro, the IC50 of SSPP-80, SSPP-80‐1 and SSPP-80‐2 against DPPH free radical were 4.407, 8.267, and 5.204 mg/mL, respectively, whereas the IC50values for removing hydroxyl groups were 5.318, 3.516, and 4.016 mg/mL, respectively. It demonstrated that SSPP-80 and 2 fractions had certain antioxidant activity. Theoretical basis for use of Sojae Semen Praeparatum polysaccharides was provided by this study.

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