scholarly journals Optimizing the ultrasonic-assisted extraction of antioxidants from Ulva lactuca algal biomass using factorial design

2021 ◽  
Author(s):  
Sayed Rashad ◽  
Ghadir El-Chaghaby ◽  
Eder C. Lima ◽  
Glaydson Simoes dos reis
Keyword(s):  
Factorial Design ◽  
Algal Biomass ◽  
Ulva Lactuca ◽  
Extraction Technique ◽  
Extraction Solvent ◽  
Assisted Extraction ◽  
Study Results ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Optimum Extraction

AbstractUltrasonic-assisted extraction (UAE) is increasingly emerging as a highly effective extraction technique. This extraction technique is affected by several experimental factors. The present work aimed to optimize the ultrasonic-assisted extraction of antioxidants from Ulva lactuca (sea lettuce), widespread macroalgae growing along the Mediterranean coast. In this respect, a full-factorial design (23) was employed to assess the effect of three different factors at two levels and their interactions on the extraction of antioxidants from sea lettuce algal biomass. The studied factors were extraction solvent, time of extraction, and temperature. The two levels chosen for extraction solvent were 100% ethanol and 50% ethanol, for the sonication time (1 h and 3 h) and temperature (25 °C and 40 °C). All experiments were done using an ultrasonic bath, and the biomass to solvent ratio was kept at 1:5. Total antioxidant capacity and quercetin concentration were set as the two responses for optimum output. The results showed that the temperature and solvent were the dominating factors that significantly affect the extraction process. The optimum extraction parameters were extraction time of 1 h, 50% ethanol, and temperature of 25 °C. Under these conditions, the maximum value for TAA was calculated as 2166.51 mg of ascorbic acid equivalent per gram (mg/g of AAE), and quercetin showed a maximum calculated value of 42.5 mg/g with combined desirability of 0.91 for the two responses. The present study results indicate that U. lactuca can be used as a source of antioxidants and phenolic compounds that can be applied in food and medicine at optimum extraction conditions. Graphical abstract

Application of factorial design in optimization of ultrasonic-assisted extraction of aluminum in juices and soft drinks

Talanta ◽  
2006 ◽  
Vol 70 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Nusrat Jalbani ◽  
Tasneem G. Kazi ◽  
Bilal M. Arain ◽  
M.K. Jamali ◽  
Hassan I. Afridi ◽  
...  
Keyword(s):  
Factorial Design ◽  
Soft Drinks ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

scholarly journals Response surface optimization of ultrasonic assisted extraction of flavonoids from Brucea javanica

2018 ◽  
Vol 238 ◽  
pp. 04005
Author(s):  
Aiping Fei ◽  
Junyu Jiang ◽  
Yong Wang ◽  
Zhigang Fang ◽  
Yingxue Teng ◽  
...  
Keyword(s):  
Response Surface ◽  
Extraction Process ◽  
Process Conditions ◽  
Brucea Javanica ◽  
Response Surface Optimization ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Surface Optimization ◽  
Optimum Extraction

This text was mainly studied the response surface optimization of ultrasonic assisted extraction of flavonoids from Brucea javanica. The optimum extraction process conditions were obtained by color reaction to prove the presence of flavonoids and by response surface simulation and quantification, the optimum condition was: ethanol concentration was 90%, the ratio of liquid to solid was 10:1, and the extraction time was 2h, the total extraction rate was the largest, up to about 1.43%.

Optimization for the Process of Chlorophyll a Extraction by Orthogonal Method

2014 ◽  
Vol 1051 ◽  
pp. 423-427
Author(s):  
Jun Sen Wu ◽  
Chen Chen Liu ◽  
Bing Li ◽  
Xiu Zhi Wan
Keyword(s):  
Chlorophyll A ◽  
Orthogonal Experiment ◽  
Ultrasonic Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Extraction Agent ◽  
Optimum Extraction ◽  
Single Factor Experiment

The 90% acetone as extraction agent used in order to obtain the ultrasonic assisted extraction conditions of chlorophyll a. The ultrasonic power, ultrasonic time, centrifugation time and rotational speed as the four study factors and the chlorophyll a concentration as the target, here, the L9(34) orthogonal table of orthogonal experiment design are used in order to determine the optimum extraction contractions, basing on the single factor experiment. The experimental results shows the algae containing water can get better extraction effect, after ultrasonic 30 minutes in 225W and 6000r/min speed centrifugal 9 minutes and then mixing, repeat ultrasound centrifugation steps.

Contrastive Experimental of the Ultrasonic-Assisted Extraction and Oscillation Extraction of Lead from Soil Using EDTA

2013 ◽  
Vol 781-784 ◽  
pp. 2301-2304
Author(s):  
Wen Cao ◽  
Xue Jin Zhou ◽  
Yun Tao Gao
Keyword(s):  
Red Soil ◽  
Extraction Rate ◽  
Extraction Time ◽  
Experimental Result ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Optimum Extraction

Ultrasonic-assisted extraction of lead from red soil using EDTA was investigated, the experimental result was compared with that of oscillation extraction to evaluate the performance of ultrasonic-assisted extraction. The optimum extraction conditions were 0.006 mol/L of EDTA, 0.006 mol/L of EDTA and 90 min of ultrasonic-assisted extraction or 240 min of oscillation extraction. Under the optimum extraction conditions, the extraction rate of 76.33% and 74.30% was obtained for ultrasonic-assisted extraction and oscillation extraction, respectively. The ultrasonic-assisted extraction could shorter the extraction time with a improved extraction rate effectively.

scholarly journals Optimization of Ultrasonic-Assisted Extraction, Characterization, and Antioxidant Activities of Polysaccharides From Sojae Semen Praeparatum

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110206
Author(s):  
Yongshuai Jing ◽  
Ruijuan Zhang ◽  
Lan Li ◽  
Danshen Zhang ◽  
Yu Liu ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Deae Cellulose ◽  
Chemical Compositions ◽  
Hydroxyl Groups ◽  
Ultrasonic Frequency ◽  
Ultrasonic Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

In this study, response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction parameters of Sojae Semen Praeparatum polysaccharides (SSPP-80), the optimum conditions were determined as follows: ultrasonic frequency of 100 W, ultrasonic power of 80 Hz, ultrasonic temperature of 52℃, ultrasonic time of 23 minutes, and liquid to raw material ratio of 40 mL/g. Based on these conditions, polysaccharides extraction rate was 7.72% ± 0.26%. Then, 2 novel polysaccharides (SSPP-80‐1, SSPP-80‐2) were isolated from SSPP by DEAE-cellulose 52 chromatography. The chemical compositions, physicochemical properties, and structure of SSPPs were investigated by simultaneous thermal analyzer (TGA), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FI-IR), and high-performance liquid chromatography (HPLC). The results showed that SSPP-80 and 2 fractions were mainly composed of mannose (Man), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara). In addition, the antioxidant activities were evaluated against the DPPH and hydroxyl radical in vitro, the IC50 of SSPP-80, SSPP-80‐1 and SSPP-80‐2 against DPPH free radical were 4.407, 8.267, and 5.204 mg/mL, respectively, whereas the IC50values for removing hydroxyl groups were 5.318, 3.516, and 4.016 mg/mL, respectively. It demonstrated that SSPP-80 and 2 fractions had certain antioxidant activity. Theoretical basis for use of Sojae Semen Praeparatum polysaccharides was provided by this study.

Sign in / Sign up

Export Citation Format

Share Document