Influence of manganese ions on cellular behavior of human osteoblasts in vitro

2007 ◽  
Vol 24 (5) ◽  
pp. 531-536 ◽  
Author(s):  
Frank Lüthen ◽  
Ulrike Bulnheim ◽  
Petra D. Müller ◽  
Joachim Rychly ◽  
Henrike Jesswein ◽  
...  
Keyword(s):  
Manganese Ions ◽  
Human Osteoblasts ◽  
Cellular Behavior

scholarly journals The Impact of Metal Ion Exposure on the Cellular Behavior of Human Osteoblasts and PBMCs: In Vitro Analyses of Osteolytic Processes

Materials ◽  
2017 ◽  
Vol 10 (7) ◽  
pp. 734 ◽  
Author(s):  
Anika Jonitz-Heincke ◽  
Jenny Tillmann ◽  
Annett Klinder ◽  
Simone Krueger ◽  
Jan Kretzer ◽  
...  
Keyword(s):  
Metal Ion ◽  
Human Osteoblasts ◽  
Cellular Behavior ◽  
The Impact

Cyanogenic glycoside amygdalin influences functions of human osteoblasts in vitro

2021 ◽  
Vol 56 (2) ◽  
pp. 109-116
Author(s):  
Radoslav Omelka ◽  
Veronika Kovacova ◽  
Vladimira Mondockova ◽  
Birgit Grosskopf ◽  
Adriana Kolesarova ◽  
...  
Keyword(s):  
Cyanogenic Glycoside ◽  
Human Osteoblasts

The effect of scaffold architecture on cellular behavior in vitro

2020 ◽  
Author(s):  
V. Elagin ◽  
S. Rodimova ◽  
N. Minaev ◽  
A. Shpichka ◽  
M. Karabut ◽  
...  
Keyword(s):  
Cellular Behavior ◽  
Scaffold Architecture

scholarly journals Adhesion Behaviour of Primary Human Osteoblasts and Fibroblasts on Polyether Ether Ketone Compared with Titanium under In Vitro Lipopolysaccharide Incubation

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2739 ◽  
Author(s):  
Korbinian Benz ◽  
Andreas Schöbel ◽  
Marisa Dietz ◽  
Peter Maurer ◽  
Jochen Jackowski
Keyword(s):  
Gene Expression ◽  
Protein Level ◽  
Binding Protein ◽  
Sem Images ◽  
Human Osteoblasts ◽  
Polyether Ether Ketone ◽  
Primary Human Osteoblasts ◽  
Titanium Surfaces ◽  
Ether Ketone

The aim of this in vitro pilot study was to analyse the adhesion behaviour of human osteoblasts and fibroblasts on polyether ether ketone (PEEK) when compared with titanium surfaces in an inflammatory environment under lipopolysaccharide (LPS) incubation. Scanning electron microscopy (SEM) images of primary human osteoblasts/fibroblasts on titanium/PEEK samples were created. The gene expression of the LPS-binding protein (LBP) and the LPS receptor (toll-like receptor 4; TLR4) was measured by real-time polymerase chain reaction (PCR). Immunocytochemistry was used to obtain evidence for the distribution of LBP/TLR4 at the protein level of the extra-cellular-matrix-binding protein vinculin and the actin cytoskeleton. SEM images revealed that the osteoblasts and fibroblasts on the PEEK surfaces had adhesion characteristics comparable to those of titanium. The osteoblasts contracted under LPS incubation and a significantly increased LBP gene expression were detected. This was discernible at the protein level on all the materials. Whereas no increase of TLR4 was detected with regard to mRNA concentrations, a considerable increase in the antibody reaction was detected on all the materials. As is the case with titanium, the colonisation of human osteoblasts and fibroblasts on PEEK samples is possible under pro-inflammatory environmental conditions and the cellular inflammation behaviour towards PEEK is lower than that of titanium.

In vitro evaluation of the direct effect of estradiol on human osteoblasts (HOB) and human mesenchymal stem cells (h-MSCs)

Injury ◽  
2006 ◽  
Vol 37 (3) ◽  
pp. S33-S42 ◽  
Author(s):  
Lucy DiSilvio ◽  
Jacqueline Jameson ◽  
Zakareya Gamie ◽  
Peter V. Giannoudis ◽  
Eleftherios Tsiridis
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Direct Effect ◽  
Human Mesenchymal Stem Cells ◽  
Human Osteoblasts ◽  
In Vitro Evaluation

scholarly journals Physical and Biological Characterization of Ferromagnetic Fiber Networks: Effect of Fibrin Deposition on Short-Term In Vitro Responses of Human Osteoblasts

2015 ◽  
Vol 21 (3-4) ◽  
pp. 463-474 ◽  
Author(s):  
Rose L. Spear ◽  
Brajith Srigengan ◽  
Suresh Neelakantan ◽  
Wolfram Bosbach ◽  
Roger A. Brooks ◽  
...  
Keyword(s):  
Biological Characterization ◽  
Fibrin Deposition ◽  
Short Term ◽  
Human Osteoblasts ◽  
Fiber Networks

In vitro Growth Pattern of Primary Human Osteoblasts on Calcium Phosphate- and Polymethylmethacrylate-Based Bone Cement

2017 ◽  
Vol 58 (5-6) ◽  
pp. 216-226
Author(s):  
Johannes Schauwecker ◽  
Mark Bock ◽  
Florian Pohlig ◽  
Heinz Mühlhofer ◽  
Jutta Tübel ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Calcium Phosphate ◽  
Cell Viability ◽  
Proliferation Rate ◽  
Control Analysis ◽  
Implant Loosening ◽  
Human Osteoblasts ◽  
Apoptosis Rate ◽  
Primary Human Osteoblasts

Background/Purpose: Polymethylmethacrylate (PMMA) and calcium phosphate (Ca-P) cements are widely used for arthroplasty surgery and augmentation of bone defects. However, aseptic implant loosening in absence of wear-induced osteolysis indicates an unfavourable interaction between the cement surface and human osteoblasts. Our underlying hypothesis is that cement surfaces directly modify cell viability, proliferation rate, and cell differentiation. Methods: To test this hypothesis, we examined primary human osteoblasts harvested from six individuals. These cells were pooled and subsequently seeded directly on cement pellets prepared from Palacos® R, Palacos® R+G, and Norian® Drillable cements. After incubation for 24 and 72 h, cell viability, proliferation rate, apoptosis rate, and cell differentiation were analysed. Results: Upon cultivation of human osteoblasts on cement surfaces, we observed a significantly reduced cell viability and DNA content compared to the control. Analysis of the apoptosis rate revealed an increase for cells on Palacos R and Norian Drillable, but a significant decrease on Palacos R+G compared to the control. Regarding osteogenic differentiation, significantly lower values of alkaline phosphatase enzyme activity were identified for all cement surfaces after 24 and 72 h compared to cultivation on tissue culture plastic, serving as control. Conclusions: In summary, these data suggest a limited biocompatibility of both PMMA and Ca-P cements, necessitating further research to reduce unfavourable cell-cement interactions and consequently extend implant survival.

Mn Blended Hydroxyapatite Nanoceramic: Bioactivity, Dielectric and Luminescence Studies

2013 ◽  
Vol 18 ◽  
pp. 43-59
Author(s):  
Megha Mahabole ◽  
Manjushree Bahir ◽  
Rajendra Khairnar
Keyword(s):  
Dielectric Constant ◽  
Calcium Ions ◽  
Nucleation And Growth ◽  
Partial Replacement ◽  
Apatite Structure ◽  
Manganese Ions ◽  
Photoluminescence Properties ◽  
In Vitro Bioactivity ◽  
Sbf Solution

Abstract: In this study, in-vitro bioactivity of manganese blended hydroxyapatite (Mn-HAp) pellets is carried out using simulated body fluid (SBF) solution. The incubated Mn-HAp samples are characterized by XRD, FTIR and SEM/EDAX. Dielectric and photoluminescence properties of Mn-HAp samples are studied as a function of incubation period in SBF. XRD profiles show that hexagonal apatite structure remains intact after partial replacement of calcium ions by manganese ions and even after incubation. The change in absorption due to phosphate group, depicted in FTIR spectra, for incubated samples confirms growth of apatite on Mn-HAp surface. SEM/ EDAX studies suggest that Mn-HAp surface promotes the growth of apatite without changing its structure due to apatite nucleation and growth on the surface of Mn-HAp. The value of dielectric constant of Mn-HAp increases after incubation. Increase in period of immersion in m-SBF leads to decrease in dielectric constant of manganese exchanged hydroxyapatite. The photoluminescence (PL) study reveals that the Mn-HAp can be used stable and efficient blue luminescent material.

scholarly journals The effect of cationically-modified phosphorylcholine polymers on human osteoblasts in vitro and their effect on bone formation in vivo

2017 ◽  
Vol 28 (9) ◽  
Author(s):  
Jonathan M. Lawton ◽  
Mariam Habib ◽  
Bingkui Ma ◽  
Roger A. Brooks ◽  
Serena M. Best ◽  
...  
Keyword(s):  
Bone Formation ◽  
Human Osteoblasts
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