Molecular cloning, expression and characterization of 100K gene of fowl adenovirus-4 for prevention and control of hydropericardium syndrome

Biologicals ◽  
2016 ◽  
Vol 44 (1) ◽  
pp. 19-23 ◽  
Author(s):  
M.S. Shah ◽  
A. Ashraf ◽  
M.I. Khan ◽  
M. Rahman ◽  
M. Habib ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Prevention And Control ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
And Control

scholarly journals Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1163
Author(s):  
So-Youn Youn ◽  
Ji-Youn Lee ◽  
You-Chan Bae ◽  
Yong-Kuk Kwon ◽  
Hye-Ryoung Kim
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Prevention ◽  
South Korea ◽  
Prevention And Control ◽  
Genetic Recombination ◽  
Detection Methods ◽  
Infectious Bronchitis ◽  
Reference Sequences ◽  
And Control

Infectious bronchitis viruses (IBVs) are evolving continuously via genetic drift and genetic recombination, making disease prevention and control difficult. In this study, we undertook genetic and pathogenic characterization of recombinant IBVs isolated from chickens in South Korea between 2003 and 2019. Phylogenetic analysis showed that 46 IBV isolates belonged to GI-19, which includes nephropathogenic IBVs. Ten isolates formed a new cluster, the genomic sequences of which were different from those of reference sequences. Recombination events in the S1 gene were identified, with putative parental strains identified as QX-like, KM91-like, and GI-15. Recombination detection methods identified three patterns (rGI-19-I, rGI-19-II, and rGI-19-III). To better understand the pathogenicity of recombinant IBVs, we compared the pathogenicity of GI-19 with that of the rGI-19s. The results suggest that rGI-19s may be more likely to cause trachea infections than GI-19, whereas rGI-19s were less pathogenic in the kidney. Additionally, the pathogenicity of rGI-19s varied according to the genotype of the major parent. These results indicate that genetic recombination between heterologous strains belonging to different genotypes has occurred, resulting in the emergence of new recombinant IBVs in South Korea.

scholarly journals Moving from rhetoric to action: how Africa can use scientific evidence to halt the COVID-19 pandemic

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Olushayo Oluseun Olu ◽  
Joy Luba Lomole Waya ◽  
Sylvester Maleghemi ◽  
John Rumunu ◽  
David Ameh ◽  
...  
Keyword(s):  
Prevention And Control ◽  
Preventive Measures ◽  
Negative Impact ◽  
Control Strategies ◽  
Scientific Evidence ◽  
Scale Up ◽  
High Impact ◽  
The Social ◽  
And Control

Abstract The ongoing pandemic of the coronavirus disease 2019 has spread rapidly to all countries of the world. Africa is particularly predisposed to an escalation of the pandemic and its negative impact given its weak economy and health systems. In addition, inadequate access to the social determinants of health such as water and sanitation and socio-cultural attributes may constrain the implementation of critical preventive measures such as hand washing and social distancing on the continent. Given these facts, the continent needs to focus on targeted and high impact prevention and control strategies and interventions which could break the chain of transmission quickly. We conclude that the available body of scientific evidence on the coronavirus disease 2019 holds the key to the development of such strategies and interventions. Going forward, we recommend that the African research community should scale up research to provide scientific evidence for a better characterization of the epidemiology, transmission dynamics, prevention and control of the virus on the continent.

scholarly journals Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jingyi Liu ◽  
Nan Mei ◽  
Yaling Wang ◽  
Xinjin Shi ◽  
Hongjun Chen
Keyword(s):  
Amino Acid Sequence Analysis ◽  
Serum Samples ◽  
Protein Amino Acid ◽  
Adenovirus Serotype ◽  
Linear Peptide ◽  
Hydropericardium Syndrome ◽  
Hexon Protein ◽  
Fowl Adenovirus ◽  
Fowl Adenovirus Serotype 4

AbstractFowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.

scholarly journals Airborne Transmission of a Serotype 4 Fowl Adenovirus in Chickens

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 262 ◽  
Author(s):  
Gang Li ◽  
Guanliu Yu ◽  
Yujuan Niu ◽  
Yumei Cai ◽  
Sidang Liu
Keyword(s):  
Infectious Disease ◽  
Infected Group ◽  
Economic Losses ◽  
Control Group ◽  
Airborne Transmission ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Viral Aerosol ◽  
Subclinical Symptoms ◽  
And Control

Serotype 4 fowl adenovirus (FAdV-4) is the main pathogen for hydropericardium syndrome (HPS) in chickens. It has caused major economic losses in the global poultry industry. Currently, FAdV-4′s transmission routes in chickens remain unclear. Here we investigate the airborne transmission routes of FAdV-4 in chickens. A total of 45 ten-day-old chickens were equally divided into three groups (infected group/isolator A, airborne group/isolator B, and control group/isolator C). Of note, isolators A and B were connected by a leak-free pipe. The results showed that the virus could form a viral aerosol, detected in isolators two days post infection (dpi). The viral aerosol reached a peak at 4 dpi in the infected group. Healthy chickens in the airborne group were infected by the virus at 8 dpi. The chickens of the airborne group demonstrated subclinical symptoms capable of shedding the virus for some time. This finding suggests that FAdV-4 can be efficiently transmitted among chickens by aerosol transmission. These findings have significant implications for developing strategies to control this infectious disease epidemic.

Characterization of the live salmonid movement network in Ireland: Implications for disease prevention and control

2015 ◽  
Vol 122 (1-2) ◽  
pp. 195-204 ◽  
Author(s):  
T. Yatabe ◽  
S.J. More ◽  
F. Geoghegan ◽  
C. McManus ◽  
A.E. Hill ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Prevention And Control ◽  
And Control

scholarly journals Why New Vaccines for the Control of Ectoparasite Vectors Have Not Been Registered and Commercialized?

Vaccines ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 75 ◽  
Author(s):  
José de la Fuente ◽  
Agustín Estrada-Peña
Keyword(s):  
Global Health ◽  
Molecular Interactions ◽  
Prevention And Control ◽  
Vector Borne Diseases ◽  
Recent Advances ◽  
Vector Borne ◽  
A Priority ◽  
And Control ◽  
New Vaccines

The prevention and control of vector-borne diseases is a priority for improving global health. Despite recent advances in the characterization of ectoparasite-host-pathogen molecular interactions, vaccines are not available for most ectoparasites and vector-borne diseases that cause millions of deaths yearly. In this paper, in response to the question of why new vaccines for the control of ectoparasite vectors have not been registered and commercialized, and to contribute developing new effective vaccines against ectoparasite vectors, we propose challenges and approaches to be addressed.

scholarly journals Characterization and pathogenicity of fowl adenovirus serotype 4 isolated from eastern China

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Kai Wang ◽  
Haiwei Sun ◽  
Yunzhang Li ◽  
Zhiwei Yang ◽  
Jianqiang Ye ◽  
...  
Keyword(s):  
Eastern China ◽  
Economic Loss ◽  
Adenovirus Serotype ◽  
Viral Loads ◽  
Cytokine Profiles ◽  
Hydropericardium Syndrome ◽  
Fowl Adenovirus ◽  
Tissue Tropisms ◽  
And Control ◽  
Fowl Adenovirus Serotype 4

Abstract Background Fowl adenovirus outbreaks have occurred in China since June 2015. This virus is an emerging infectious disease that causes hydropericardium syndrome and inclusion body hepatitis (HPS-IBH), resulting in significant economic loss to poultry farmers. Five fowl adenovirus (FAdV) strains (HN, AQ, AH726, JS07 and AH712) were isolated from Jiangsu and Anhui provinces. Results Phylogenetic analysis revealed that the five isolates belonged to species C fowl adenovirus serotype 4. An 11 amino-acid deletion in ORF29, relative to an older viral isolate, JSJ13, was observed for all five strains described here. In chicken experiments, 80–100% birds died after intramuscular inoculation and displayed lesions characteristic of HPS-IBH. The viral DNA copies were further detected by hexon-probe based real-time polymerase chain reaction (PCR) in the chicken samples. The viral loads and cytokine profiles were recorded in all the organs after infections. Despite minor genetic differences, the 5 strains displayed significantly different tissue tropisms and cytokine profiles. Conclusions Our data enhance the current understanding some of the factors involved in the pathogenicity and genetic diversity of the FAdV serotype 4 (FAdV-4) in China. Our work provides theoretical support for the prevention and control of HPS-IBH in chickens.

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