scholarly journals Modulation of anisotropy in electrospun tissue-engineering scaffolds: Analysis of fiber alignment by the fast Fourier transform

Biomaterials ◽  
2006 ◽  
Vol 27 (32) ◽  
pp. 5524-5534 ◽  
Author(s):  
Chantal Ayres ◽  
Gary L. Bowlin ◽  
Scott C. Henderson ◽  
Leander Taylor ◽  
Jackie Shultz ◽  
...  
2008 ◽  
Vol 19 (5) ◽  
pp. 603-621 ◽  
Author(s):  
Chantal E. Ayres ◽  
B. Shekhar Jha ◽  
Hannah Meredith ◽  
James R. Bowman ◽  
Gary L. Bowlin ◽  
...  

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2479
Author(s):  
Bo Kyung Park ◽  
In Chul Um

Recently, the electrospun silk web has been intensively studied in terms of its biomedical applications, including tissue engineering scaffolds, due to its good biocompatibility, cytocompatibility, and biodegradability. In this study, the effect of relative humidity (RH) conditions on the morphology of electrospun silk fiber and the electrospinning production rate of silk solution was examined. In addition, the effect of RH on the molecular conformation of electrospun silk web was examined using Fourier transform infrared (FTIR) spectroscopy. As RH was increased, the maximum electrospinning rate of silk solution and fiber diameter of the resultant electrospun silk web were decreased. When RH was increased to 60%, some beads were observed, which showed that the electrospinnability of silk formic acid solution deteriorated with an increase in RH. The FTIR results showed that electrospun silk web was partially β-sheet crystallized and RH did not affect the molecular conformation of silk.


2011 ◽  
Vol 17 (2) ◽  
pp. 156-166 ◽  
Author(s):  
Steven Frank Kemeny ◽  
Alisa Morss Clyne

AbstractFiber alignment plays a critical role in the structure and function of cells and tissues. While fiber alignment quantification is important to experimental analysis and several different methods for quantifying fiber alignment exist, many studies focus on qualitative rather than quantitative analysis perhaps due to the complexity of current fiber alignment methods. Speed and sensitivity were compared in edge detection and fast Fourier transform (FFT) for measuring actin fiber alignment in cells exposed to shear stress. While edge detection using matrix multiplication was consistently more sensitive than FFT, image processing time was significantly longer. However, when MATLAB functions were used to implement edge detection, MATLAB's efficient element-by-element calculations and fast filtering techniques reduced computation cost 100 times compared to the matrix multiplication edge detection method. The new computation time was comparable to the FFT method, and MATLAB edge detection produced well-distributed fiber angle distributions that statistically distinguished aligned and unaligned fibers in half as many sample images. When the FFT sensitivity was improved by dividing images into smaller subsections, processing time grew larger than the time required for MATLAB edge detection. Implementation of edge detection in MATLAB is simpler, faster, and more sensitive than FFT for fiber alignment quantification.


2019 ◽  
Author(s):  
AS Arampatzis ◽  
K Theodoridis ◽  
E Aggelidou ◽  
KN Kontogiannopoulos ◽  
I Tsivintzelis ◽  
...  

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.


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