ABSTRACTA piezoelectric drop on demand printer has been used to print primary human osteoblast and bovine chondrocyte cells. After deposition the cells were incubated at 37°C and characterised using optical microscopy, SEM and cell viability assays. Cells showed a robust response to printing exhibiting signs of proliferation and spreading. Increasing the drop velocity results in a reduced cell survival and proliferation rates but both cell types grew to confluence after printing under all conditions studied.
Analysis of the dexamethasone (DEX), BMP2, TNFA, IGF1, prostaglandin E2 (PGE2) and 1,25 dihydroxyvitamin D3 (125DVD3)-regulated transcriptome in primary human osteoblast cultures