Pre-vascularization of in vitro three-dimensional tissues created by cell sheet engineering

Biomaterials ◽  
2010 ◽  
Vol 31 (14) ◽  
pp. 3903-3909 ◽  
Author(s):  
Nahoko Asakawa ◽  
Tatsuya Shimizu ◽  
Yukiko Tsuda ◽  
Sachiko Sekiya ◽  
Tadashi Sasagawa ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
Cell Sheet Engineering

Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer

2021 ◽  
pp. 088532822110278
Author(s):  
Atakan Tevlek ◽  
Halil Murat Aydin
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
Osteogenic Cell ◽  
Electrospun Membrane ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Sulfated Glycosaminoglycan ◽  
Sgag Content ◽  
In Vitro Performance

In this study, it was aimed to present an approach for the development of multi-layered tissue engineering constructs by using cell sheet engineering. Briefly, MC3T3-E1 mouse pre-osteoblast cells were cultured in temperature-responsive plates (Nunc Upcell®) in the presence of osteogenic medium and the resulting cell sheets were laminated with electrospun poly(L-lactic acid) (PLLA) membranes to obtain viable three-dimensional, thick constructs. The constructs prepared without PLLA membranes were used as control. The cell viability and death in the resulting structures were investigated by microscopic and colorimetric methods. The in vitro performance of the structures was discussed comparatively. Alkaline phosphatase (ALP) activity, collagen and sulfated glycosaminoglycan (sGAG) content values were calculated. The presented approach shows potential for engineering applications of complex tissues with at least two or more microenvironments such as osteochondral, corneal or vascular tissues.

Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation

2015 ◽  
Vol 103 (12) ◽  
pp. 3825-3833 ◽  
Author(s):  
Akiyuki Hasegawa ◽  
Yuji Haraguchi ◽  
Tatsuya Shimizu ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
Cell Sheet Engineering ◽  
Rapid Fabrication

scholarly journals A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
A. Cagdas Yorukoglu ◽  
A. Esat Kiter ◽  
Semih Akkaya ◽  
N. Lale Satiroglu-Tufan ◽  
A. Cevik Tufan
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethical Issues ◽  
Cell Sheet ◽  
Bone Tissue Regeneration ◽  
Differentiation Potential ◽  
Cell Sheet Engineering ◽  
Application Techniques

The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.

scholarly journals In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair

2018 ◽  
Vol 19 (8) ◽  
pp. 2272 ◽  
Author(s):  
Chi-Fen Hsieh ◽  
Zexing Yan ◽  
Ricarda Schumann ◽  
Stefan Milz ◽  
Christian Pfeifer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Sheet ◽  
Tendon Repair ◽  
Three Dimensional ◽  
Cell Types ◽  
Tendon Tissue ◽  
Cell Sheets ◽  
Tendon Tissue Engineering ◽  
Dimensional Cell

The poor and slow healing capacity of tendons requires novel strategies to speed up the tendon repair process. Hence, new and promising developments in tendon tissue engineering have become increasingly relevant. Previously, we have established a tendon progenitor cell line via ectopic expression of the tendon-related basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) in human bone marrow mesenchymal stem cells (hMSC-Scx). The aim of this study was to directly compare the characteristics of hMSC-Scx cells to that of primary human tendon stem/progenitors cells (hTSPCs) via assessment of self-renewal and multipotency, gene marker expression profiling, in vitro wound healing assay and three-dimensional cell sheet formation. As expected, hTSPCs were more naive than hMSC-Scx cells because of higher clonogenicity, trilineage differentiation potential, and expression of stem cell markers, as well as higher mRNA levels of several gene factors associated with early tendon development. Interestingly, with regards to wound healing, both cell types demonstrate a comparable speed of scratch closure, as well as migratory velocity and distance in various migration experiments. In the three-dimensional cell sheet model, hMSC-Scx cells and hTSPCs form compact tendinous sheets as histological staining, and transmission electron microscopy shows spindle-shaped cells and collagen type I fibrils with similar average diameter size and distribution. Taken together, hTSPCs exceed hMSC-Scx cells in several characteristics, namely clonogenicity, multipotentiality, gene expression profile and rates of tendon-like sheet formation, whilst in three-dimensional cell sheets, both cell types have comparable in vitro healing potential and collagenous composition of their three-dimensional cell sheets, making both cell types a suitable cell source for tendon tissue engineering and healing.

Reconstruction of functional endometrium-like tissue in vitro and in vivo using cell sheet engineering

2014 ◽  
Vol 446 (1) ◽  
pp. 335-340 ◽  
Author(s):  
Soichi Takagi ◽  
Tatsuya Shimizu ◽  
Goro Kuramoto ◽  
Ken Ishitani ◽  
Hideo Matsui ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Cell Sheet Engineering

scholarly journals Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Liling Ren ◽  
Dongyang Ma ◽  
Bin Liu ◽  
Jinda Li ◽  
Jia Chen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Vessels ◽  
Tissue Regeneration ◽  
Umbilical Vein ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Human Umbilical Vein ◽  
Immunodeficient Mice ◽  
Umbilical Vein Endothelial Cells

Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to form a thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to form a 3D construct by a modified cell sheet engineering technique.In vitroresults indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions.In vivoresults showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed thatin vitropreformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.

Cell Sheet-Based Vascularized Myocardial Tissue Fabrication

2018 ◽  
Vol 59 (3-4) ◽  
pp. 276-285 ◽  
Author(s):  
Hyoe Komae ◽  
Minoru Ono ◽  
Tatsuya Shimizu
Keyword(s):  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Myocardial Tissue ◽  
Engineering Technology ◽  
Cell Sheet Engineering ◽  
Human Cardiomyocytes ◽  
Whole Heart

Background: The development of regenerative medicine in recent years has been remarkable as tissue engineering technology and stem cell research have advanced. The ultimate goal of regenerative medicine is to fabricate human organs artificially. If fabricated organs can be transplanted medically, it will be the innovative treatment of diseases for which only donor organ transplantation is the definitive therapeutic method at present. Summary: Our group has reported successful fabrication of thick functional myocardial tissue in vivo and in vitro by using cell sheet engineering technology which requires no scaffolds. Thick myocardial tissue can be fabricated by stacking cardiomyocyte sheets on the vascular bed every 24 h, so that a vascular network can be formed within the myocardial graft. We call this procedure a multi-step transplantation procedure. After human-induced pluripotent stem cells were discovered and human cardiomyocytes became available, a thick, macroscopically pulsate human myocardial tissue was successfully constructed by using a multi-step transplantation procedure. Furthermore, our group succeeded in fabricating functional human myocardial tissue which can generate pressure. Here, we present our way of fabricating human myocardial tissue by means of cell sheet engineering technology. Key Messages: Our group succeeded in fabricating thick, functional human myocardium which can generate pulse pressure. However, there are still a few problems to be solved until clinically functional human cardiac tissue or a whole heart can be fabricated. Research on myocardial regeneration progresses at such a pace that we believe the products of this research will save many lives in the near future.

scholarly journals In Vitro Production of Human Ballooned Hepatocytes in a Cell Sheet-based Three-dimensional Model

2020 ◽  
Vol 26 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Botao Gao ◽  
Katsuhisa Sakaguchi ◽  
Katsuhisa Matsuura ◽  
Tetsuya Ogawa ◽  
Yuki Kagawa ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
Dimensional Model ◽  
In Vitro Production ◽  
Three Dimensional Model ◽  
A Cell ◽  
Vitro Production
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