Harnessing in situ glutathione for effective ROS generation and tumor suppression via nanohybrid-mediated catabolism dynamic therapy

Abstract The growth of undesired bacteria causes numerous problems. Here, we show that locally enhanced electric field treatment (LEEFT) can cause rapid bacteria inactivation by electroporation without any side reactions. The bacteria inactivation is studied in situ at the single-cell level on a lab-on-a-chip that has nanowedge-decorated electrodes. Rapid bacteria inactivation occurs specifically at nanowedge tips where the electric field is enhanced due to the lightning-rod effect. The mechanism study shows that the bacteria inactivation is caused by electroporation induced by the locally enhanced electric field. The bacteria inactivation performance depends on the strength of the enhanced electric field instead of the applied voltage, and no ROS generation is detected when >90% bacteria inactivation is achieved. Quick membrane pore closure under moderate LEEFT indicates that electroporation is the predominant mechanism. LEEFT only requires facile treatment to achieve bacteria inactivation, which is safe for treating delicate samples and energy-efficient for large scale applications. The findings in this work can provide strong supports for the future applications of LEEFT.

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A smart nano-V2O5/ODA-coated mesh for a co-responsive photo-induced wettability transition and ROS generation for in situ water purification

Journal of Materials Chemistry A ◽

10.1039/c8ta07094f ◽

2018 ◽

Vol 6 (37) ◽

pp. 18003-18009 ◽

Cited By ~ 14

Author(s):

Ruixiang Qu ◽

Weifeng Zhang ◽

Xiangyu Li ◽

Yanan Liu ◽

Tzungyu Shih ◽

...

Keyword(s):

Water Purification ◽

Ros Generation

A smart nano-V2O5/ODA-coated mesh with co-responsive photo-induced wettability transition and ROS generation was fabricated.

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Targeting Oxidative Stress Injury after Ischemic Stroke in Conscious Rats: Limited Benefits with Apocynin Highlight the Need to Incorporate Long Term Recovery

Stroke Research and Treatment ◽

10.1155/2013/648061 ◽

2013 ◽

Vol 2013 ◽

pp. 1-13 ◽

Cited By ~ 3

Author(s):

Robert M. Weston ◽

Bin Lin ◽

Gregory J. Dusting ◽

Carli L. Roulston

Keyword(s):

Oxidative Stress ◽

Infarct Volume ◽

Neurological Deficits ◽

Ros Generation ◽

Protective Effects ◽

Conscious Rat ◽

Stress Injury ◽

Cell Responses

NADPH oxidase is a major source of superoxide anion following stroke and reperfusion. This study evaluated the effects of apocynin, a known antioxidant and inhibitor of Nox2 NADPH, on neuronal injury and cell-specific responses to stroke induced in the conscious rat. Apocynin treatment (50 mg/kg i.p.) commencing 1 hour prior to stroke and 24 and 48 hours after stroke significantly reduced infarct volume in the cortex by~ 60%, but had no effect on striatal damage or neurological deficits.In situdetection of reactive oxygen species (ROS) using dihydroethidium fluorescence revealed that increased ROS detected in OX-42 positive cells following ischemia was reduced in apocynin-treated rats by~ 51%, but surprisingly increased in surrounding NeuN positive cells of the same rats by~ 27%, in comparison to the contralateral hemisphere. Reduced ROS from activated microglia/macrophages treated with apocynin was associated with reduced Nox2 immunoreactivity without change to the number of cells. These findings confirm the protective effects of apocynin and indicate a novel mechanism via reduced Nox2 expression. We also reveal compensatory changes in neuronal ROS generation as a result of Nox2 inhibition and highlight the need to assess long term individual cell responses to inhibitors of oxidative stress.

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In Situ Generation of Hydrogen Peroxide Using Polymetallic-Doped g-C3N4 for Pollutant Removal

Applied Sciences ◽

10.3390/app112210797 ◽

2021 ◽

Vol 11 (22) ◽

pp. 10797

Author(s):

Liyan Wang ◽

Jianqing Ma ◽

Qianhui Guo ◽

Liang Liu ◽

Jiangnan Shou ◽

...

Keyword(s):

Organic Contaminants ◽

Economic Benefits ◽

Pollutant Removal ◽

High Price ◽

Ros Generation ◽

Ternary Metal ◽

Pollutants Removal ◽

Acid Red 73 ◽

Metal Doped

Fenton reaction is a powerful technology for pollutants’ removal from water. However, the cost of H2O2 becomes one of the major stumbling blocks in its application. H2O2 has a relatively high price and is easily decomposed during transportation and use; therefore, in situ synthesis of H2O2 could improve economic benefits effectively. In this study, a Fe/Ni/Pd ternary metal-doped graphitic carbon nitride (FeNi-Pd@CN) is prepared, and in situ H2O2 generation using formic acid as hydrogen sources for organics removal was proved. The catalyst is advantageous, as H2O2 could accumulate to 1.69 mmol/L in 150 min when pumping air rather than oxygen gases in other studies. Furthermore, 92.0% of Acid Red 73 (200 mg/L) and 93.2% of tetracycline hydrochloride (10 mg/L) could be removed in 150 min without any pH adjustment. Characterization results show that the catalyst has good stability in metal leaching and reuse tests. It is proved that •OH and •O2− are the main reactive oxygen species, and a synergistic effect between Fe and Ni exists that enhances ROS generation for organics degradation. This work offers a promising method to remove refectory organic contaminants from industrial wastewater.

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Site-specific sonocatalytic tumor suppression by chemically engineered single-crystalline mesoporous titanium dioxide sonosensitizers

Journal of Materials Chemistry B ◽

10.1039/c7tb00938k ◽

2017 ◽

Vol 5 (24) ◽

pp. 4579-4586 ◽

Cited By ~ 29

Author(s):

Xi Wang ◽

Wenping Wang ◽

Luodan Yu ◽

Yang Tang ◽

Jiaying Cao ◽

...

Keyword(s):

Titanium Dioxide ◽

Mesoporous Silica ◽

Tumor Suppression ◽

Site Specific ◽

Single Crystalline ◽

Dynamic Therapy ◽

Mesoporous Titanium Dioxide

Mesoporous silica nanocrystals have been developed as sonosensitizers for efficient dynamic therapy of tumors.

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Cr(V)–Cr(III) in-situ transition promotes ROS generation to achieve efficient cancer therapy

Biomaterials ◽

10.1016/j.biomaterials.2021.120991 ◽

2021 ◽

pp. 120991

Author(s):

Mingkai Chen ◽

Xiaoting Huang ◽

Huatian Shi ◽

Jie Lai ◽

Li Ma ◽

...

Keyword(s):

Cancer Therapy ◽

Ros Generation

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The role of reactive oxygen species in the immunity induced by nano-pulse stimulation

Scientific Reports ◽

10.1038/s41598-021-03342-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Siqi Guo ◽

Niculina I. Burcus ◽

Megan Scott ◽

Yu Jing ◽

Iurii Semenov

Keyword(s):

Cancer Cells ◽

Breast Cancer Cells ◽

Ros Generation ◽

Ros Production ◽

Oxygen Species ◽

Ros Scavengers ◽

Pulse Stimulation

AbstractReactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated the role of NPS induced ROS in antitumor immunity. ROS production in 4T1-luc breast cancer cells was characterized using three detection reagents, namely, Amplex Red, MitoSox Red, and Dihydroethidium. The efficiency of ROS quenching was evaluated in the presence or absence of ROS scavengers and/or antioxidants. The immunogenicity of NPS treated tumor cells was assessed by ex vivo dendritic cell activation, in vivo vaccination assay and in situ vaccination with NPS tumor ablation. We found that NPS treatment enhanced the immunogenicity of 4T1-luc mouse mammary tumor, resulted in a potent in situ vaccination protection and induced long-term T cell immunity. ROS production derived from NPS treated breast cancer cells was an electric pulse dose-dependent phenomenon. Noticeably, the dynamic pattern of hydrogen peroxide production was different from that of superoxide production. Interestingly, regardless of NPS treatment, different ROS scavengers could either block or promote ROS production and stimulate or inhibit tumor cell growth. The activation of dendritic cells was not influenced by blocking ROS generation. The results from in vivo vaccination with NPS treated cancer cells suggests that ROS generation was not a prerequisite for immune protection.

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Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1606253113 ◽

2016 ◽

Vol 113 (35) ◽

pp. E5172-E5181 ◽

Cited By ~ 48

Author(s):

Michaela Finsterbusch ◽

Pam Hall ◽

Anqi Li ◽

Sapna Devi ◽

Clare L. V. Westhorpe ◽

...

Keyword(s):

Dwell Time ◽

Tissue Injury ◽

Neutrophil Recruitment ◽

Ros Generation ◽

Glomerular Injury ◽

Inflammatory Stimuli ◽

Tnf Inhibition ◽

Patrolling Monocytes ◽

Glomerular Capillaries

Nonclassical monocytes undergo intravascular patrolling in blood vessels, positioning them ideally to coordinate responses to inflammatory stimuli. Under some circumstances, the actions of monocytes have been shown to involve promotion of neutrophil recruitment. However, the mechanisms whereby patrolling monocytes control the actions of neutrophils in the circulation are unclear. Here, we examined the contributions of monocytes to antibody- and neutrophil-dependent inflammation in a model of in situ immune complex-mediated glomerulonephritis. Multiphoton and spinning disk confocal intravital microscopy revealed that monocytes patrol both uninflamed and inflamed glomeruli using β2 and α4 integrins and CX3CR1. Monocyte depletion reduced glomerular injury, demonstrating that these cells promote inappropriate inflammation in this setting. Monocyte depletion also resulted in reductions in neutrophil recruitment and dwell time in glomerular capillaries and in reactive oxygen species (ROS) generation by neutrophils, suggesting a role for cross-talk between monocytes and neutrophils in induction of glomerulonephritis. Consistent with this hypothesis, patrolling monocytes and neutrophils underwent prolonged interactions in glomerular capillaries, with the duration of these interactions increasing during inflammation. Moreover, neutrophils that interacted with monocytes showed increased retention and a greater propensity for ROS generation in the glomerulus. Also, renal patrolling monocytes, but not neutrophils, produced TNF during inflammation, and TNF inhibition reduced neutrophil dwell time and ROS production, as well as renal injury. These findings show that monocytes and neutrophils undergo interactions within the glomerular microvasculature. Moreover, evidence indicates that, in response to an inflammatory stimulus, these interactions allow monocytes to promote neutrophil recruitment and activation within the glomerular microvasculature, leading to neutrophil-dependent tissue injury.

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