Diospyros lotus leaf and grapefruit stem extract synergistically ameliorate atopic dermatitis-like skin lesion in mice by suppressing infiltration of mast cells in skin lesions

2017 ◽  
Vol 89 ◽  
pp. 819-826 ◽  
Author(s):  
Byoung Ok Cho ◽  
Denis Nchang Che ◽  
Hong Hua Yin ◽  
Jae Young Shin ◽  
Seon Il Jang
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Skin Lesion ◽  
Skin Lesions ◽  
Lotus Leaf ◽  
Diospyros Lotus ◽  
Stem Extract

scholarly journals Topical Spilanthol Inhibits MAPK Signaling and Ameliorates Allergic Inflammation in DNCB-Induced Atopic Dermatitis in Mice

2019 ◽  
Vol 20 (10) ◽  
pp. 2490 ◽  
Author(s):  
Wen-Chung Huang ◽  
Chun-Hsun Huang ◽  
Sindy Hu ◽  
Hui-Ling Peng ◽  
Shu-Ju Wu
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Protein Kinase ◽  
Allergic Inflammation ◽  
Skin Lesions ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Mapk Pathways ◽  
Mitogen Activated Protein ◽  
Cox 2

Atopic dermatitis (AD) is a recurrent allergic skin disease caused by genetic and environmental factors. Patients with AD may experience immune imbalance, increased levels of mast cells, immunoglobulin (Ig) E and pro-inflammatory factors (Cyclooxygenase, COX-2 and inducible NO synthase, iNOS). While spilanthol (SP) has anti-inflammatory and analgesic activities, its effect on AD remains to be explored. To develop a new means of SP, inflammation-related symptoms of AD were alleviated, and 2,4-dinitrochlorobenzene (DNCB) was used to induce AD-like skin lesions in BALB/c mice. Histopathological analysis was used to examine mast cells and eosinophils infiltration in AD-like skin lesions. The levels of IgE, IgG1 and IgG2a were measured by enzyme-linked immunosorbent assay (ELISA) kits. Western blot was used for analysis of the mitogen-activated protein kinase (MAPK) pathways and COX-2 and iNOS protein expression. Topical SP treatment reduced serum IgE and IgG2a levels and suppressed COX-2 and iNOS expression via blocked mitogen-activated protein kinase (MAPK) pathways in DNCB-induced AD-like lesions. Histopathological examination revealed that SP reduced epidermal thickness and collagen accumulation and inhibited mast cells and eosinophils infiltration into the AD-like lesions skin. These results indicate that SP may protect against AD skin lesions through inhibited MAPK signaling pathways and may diminish the infiltration of inflammatory cells to block allergic inflammation.

Quantitative analysis of bikunin-laden mast cells in follicular eruptions and chronic skin lesions of atopic dermatitis

2002 ◽  
Vol 294 (9) ◽  
pp. 387-392 ◽  
Author(s):  
Rieko Isogai ◽  
Akiko Matsukura ◽  
Yoshinori Aragane ◽  
Akira Maeda ◽  
Masaharu Matsukura ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Quantitative Analysis ◽  
Mast Cells ◽  
Skin Lesions ◽  
Chronic Skin

scholarly journals Anti-Inflammatory Effect of Qingpeng Ointment in Atopic Dermatitis-Like Murine Model

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yun-Zhu Li ◽  
Xue-Yan Lu ◽  
Wei Jiang ◽  
Lin-Feng Li
Keyword(s):  
Atopic Dermatitis ◽  
Chinese Medicine ◽  
Mast Cells ◽  
Mrna Expression ◽  
Murine Model ◽  
Skin Lesions ◽  
Serum Immunoglobulin ◽  
Repeated Application ◽  
Immune Dysfunctions ◽  
Inflammatory Effect

Qingpeng ointment (QP) is a Chinese medicine which has been used in treatment of atopic dermatitis (AD) in China. AD-like lesions were induced in BALB/c mice by repeated application of 2,4-dinitrofluorobenzene (DNFB) on shaved backs. The mice were then treated for 2 weeks with QP of different concentrations and Mometasone Furoate cream (MF), respectively. Macroscopic and microscopic changes of the skin lesions were observed after the treatment. The levels of serum immunoglobulin (Ig) E, tissue interferon (IFN)-γ, and interleukin (IL)-4 and IL-17A and the levels of involucrin, filaggrin, and kallikrein7 in epidermis were measured. The results show severe dermatitis with immune profiles similar to human acute AD. A significant infiltration of CD4+T and mast cells was observed in dermis of lesion but inhibited by QP after a 2-week treatment with it. The production of IgE, IL-4 and the mRNA expression of IL-17A were also suppressed, but the level of IFN-γwas increased. MF suppressed all production of these cytokines and IgE. Accordingly, the mechanism of QP on AD might correlate with its ability of modulating the immune dysfunctions rather than suppressing them. It had no effect on expressions of involucrin and filaggrin, except that its vehicle decreased the level of kallikrein7.

scholarly journals Therapeutic Effects of Chinese Herbal Formula (PTQX) on NC/Nga Mice with Atopic Dermatitis-Like Skin Lesions

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Fenggen Yan ◽  
Jing Zhang ◽  
Xiong Li ◽  
Xiumei Mo ◽  
Junfeng Liu ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Lymph Nodes ◽  
Immunoglobulin E ◽  
Skin Lesions ◽  
Therapeutic Effects ◽  
Herbal Formula ◽  
Dorsal Skin ◽  
Chinese Herbal Formula ◽  
Chinese Herbal

Atopic dermatitis (AD), also known as atopic eczema, is a chronic pruritic inflammatory skin disease. The available systemic therapies for atopic dermatitis are inadequate. Objective. This study aimed to evaluate the effects of the Chinese herbal formula Pei Tu Qing Xin (PTQX) on dermatitis severity and ear swelling, immunomodulation, and the infiltration of mast cells in a mouse model of 1-chloro-2,4-dinitrobenzene- (DNCB-) induced AD. Methods. AD-like symptoms were induced by DNCB in NC/Nga mice. Skin lesions, dermatitis, ear swelling, and scratching behaviour were evaluated. Changes in the T-helper type 1 (Th1), Th2, Th17, and regulatory T (Treg) subtypes and immunoregulation in the spleen and lymph nodes were detected by flow cytometry. Results. Histopathological and immunohistochemical analyses demonstrated that PTQX decreased the DNCB-mediated induction of mast cells and infiltration of inflammatory cells in the ear and dorsal skin. PTQX also reduced the DNCB-induced increase in the serum immunoglobulin E level, pruritus, and dermatitis (red, flaky areas) on the dorsal skin. Furthermore, PTQX regulated the balance between the populations of Th1, Th2, Th17, and Treg cells (particularly the latter two) in the lymph nodes. Conclusions. Our results suggest that the Chinese herbal formula PTQX can alleviate symptoms of AD, such as epithelial damage, redness, swelling, and pruritus, and potentially be used to treat this condition.

Inhibitory Effects of Pterocarpus santalinus Extract against IgE/Antigen-Sensitized Mast Cells and Atopic Dermatitis-Like Skin Lesions

Planta Medica ◽  
2019 ◽  
Vol 85 (07) ◽  
pp. 599-607 ◽  
Author(s):  
Baknoon Ham ◽  
Myungsuk Kim ◽  
Yang-Ju Son ◽  
Sungyul Chang ◽  
Sang Hoon Jung ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Prostaglandin E2 ◽  
Immunoglobulin E ◽  
Skin Lesions ◽  
T Helper Cell ◽  
Helper Cell ◽  
T Helper ◽  
Inhibitory Effects ◽  
Pterocarpus Santalinus

Abstract Pterocarpus santalinus has been traditionally used as a medicinal plant owing to its anti-inflammatory, anthelmintic, tonic, antihyperglycemic, and diaphoretic properties. We hypothesized that P. santalinus might have therapeutic potential in alleviating allergy and atopic dermatitis. Thus, we investigated the inhibitory effects of P. santalinus extract against allergic responses and 2,4-dinitrochlorobenzene-induced atopic dermatitis-like dorsal skin lesions using immunoglobulin E-sensitized rat basophilic leukemia-2H3 mast cells and NC/Nga mice. Degranulation and enzyme-linked immunosorbent assays were conducted to measure degranulation, proinflammatory cytokine levels, and prostaglandin E2 concentrations in immunoglobulin E/antigen-sensitized RBL-2H3 mast cells. The therapeutic efficacy of P. santalinus extract in 2,4-dinitrochlorobenzene-induced atopic dermatitis was evaluated through morphological, physiological, and immunological analysis. P. santalinus extract inhibited β-hexosaminidase and histamine release and reduced tumor necrosis factor-α, interleukin-4, and prostaglandin E2 secretion. Furthermore, P. santalinus extract suppressed atopic dermatitis-like skin lesions by regulating the serum levels of immunoglobulin E and immunoglobulin G2a, and messenger ribonucleic acid expression of T helper cell 1- and T helper cell 2-related mediators in the skin lesions. Histopathological analyses showed a decrease in epidermal thickness and intradermal inflammatory cell infiltration. These results suggested that P. santalinus extract might have beneficial effects in treating allergic and atopic dermatitis-like skin disorders.

scholarly journals Ethanol Extract of Sanguisorbae Radix Inhibits Mast Cell Degranulation and Suppresses 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Ju-Hye Yang ◽  
Jae-Myung Yoo ◽  
Won-Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Ethanol Extract ◽  
Underlying Mechanism ◽  
Skin Lesions ◽  
Mouse Bone Marrow ◽  
Mice Model ◽  
Epidermal Thickness ◽  
Sanguisorba Officinalis ◽  
Sanguisorbae Radix

Sanguisorbae Radix (SR) is well known as herbal medicine named “Zi-Yu” in Korea, which is the dried roots ofSanguisorba officinalisL. (Rosacease). We investigated the underlying mechanism on the inhibition of atopic dermatitis (AD) of an ethanol extract of SR (ESR) using 2,4-dinitrochlorobenzene- (DNCB-) induced AD mice model. Oral administration of ESR significantly suppressed DNCB-induced AD-like symptoms such as scratching behavior, ear thickness, epidermal thickness, and IgE levels. To investigate the effects of ESR treatment on degranulation of IgE/Ag-activated mouse bone marrow-derived mast cells (BMMCs), we measured the release ofβ-hexosaminidase (β-HEX, degranulation marker). ESR decreased the infiltration of eosinophils and mast cells into the AD skin lesions. Furthermore, ESR significantly inhibited degranulation of IgE/Ag-activated BMMCs. We have demonstrated that ESR decreased AD symptoms in mice and inhibits degranulation of IgE/Ag-activated mast cells. Our study suggests that ESR may serve as a potential therapeutic candidate for the treatment of AD symptoms.

scholarly journals The species differences of staphylococci isolated from skin lesions in children and adolescents with atopic dermatitis

2015 ◽  
Vol 12 (3) ◽  
pp. 41-46
Author(s):  
A V Kudryavtseva ◽  
J A Savvina ◽  
O A Morozova
Keyword(s):  
Atopic Dermatitis ◽  
Skin Lesion ◽  
Children And Adolescents ◽  
Disease Severity ◽  
Bacterial Growth ◽  
Species Differences ◽  
Antibiotic Sensitivity ◽  
Skin Lesions ◽  
Oxacillin Resistance ◽  
Inflammatory Changes

Materials and methods. This study enrolled 87 patients with AD different manifestations in its acute phase. Most children were with moderate AD. In over 50% of cases we detected the erythemato-squamous form of AD (ES). 9 patients with severe manifestations had a lichenoid form. Skin smears were taken before starting therapy. The identification of staphylococcal types was performed and their antibiotic sensitivity was tested by means of the automated microbiology analyzer «Walka-way 96 plus», Siemens Healthcare Diagnostics, Germany. Results. The study proved that S. aureus and S. epidermidis (SA and SE) were mostly isolated from skin lesion: the coagulazo-negative staphylococci (CNS) included S. haemolyticus, S. warneri, S. hominis, S. saprophyticus and S. sciuri. 12,7% cases showed no bacterial growth. No difference between SA or CNS and the disease severity was recorded. CNS were proved to colonize more frequently the skin of patients with erythemato-squamous AD while SA was a more frequent colonizer in chronic AD with lichenization (р=0,081). All staphylococci were penicillin-resistant. Among CNS, 8 out of31 SE strains, 1 S. haemolyticus and 1 S. sciuri were oxacillin-resistant. Conclusion. Therefore, we can state that alongside with SA colonization and regardless of AD severity CNS skin lesion colonization is detected in patients with AD. Moreover, CNS colonization accompanies acute inflammatory changes and in some cases demonstrates oxacillin-resistance. These findings suggest the need for further research into the role that CNS play in AD progression.

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