Nephroprotective effect of naringin in methotrexate induced renal toxicity in male rats

Methotrexate (MTX) is frequently used drug in treatment of cancer and autoimmune diseases. Unfortunately, MTX has many side effects including the hepato-renal toxicity. In this study, we hypothesized that Luteolin (Lut) exhibits protective effect against the MTX-induced hepato-renal toxicity. In order to investigate our hypothesis, the experiment was designed to examine the effect of exposure of male rats to MTX (20 mg/kg, i.p., at day 9) alone or together with Lut (50 mg/kg, oral for 14 days) compared to the control rats (received saline). The findings demonstrated that MTX treatment induced significant increases in the liver and kidney functions markers in serum samples including Aspartate transaminase (AST), Alanine transaminase (ALT), creatinine, urea and uric acid. MTX also mediated an oxidative stress expressed by elevated malondialdehyde (MDA) level and decreased level of reduced glutathione (GSH), antioxidant enzyme activities, and downregulation of the Nrf2 gene expression as an antioxidant trigger. Moreover, the inflammatory markers (NF-κB, TNF-α, and IL-1β) were significantly elevated upon MTX treatment. In addition, MTX showed an apoptotic response mediated by elevating the pro-apoptotic (Bax) and lowering the anti-apoptotic (Bcl-2) proteins. All of these changes were confirmed by the observed alterations in the histopathological examination of the hepatic and renal tissues. Lut exposure significantly reversed all the MTX-induced changes in the measured parameters suggesting its potential protective role against the MTX-induced toxicity. Finally, our findings concluded the antioxidative, anti-inflammatory and anti-apoptotic effects of Lut as a mechanism of its protective role against the MTX-induced hepato-renal toxicity in rats.

Download Full-text

Effects of Lipid-Based Oral Formulations on Plasma and Tissue Amphotericin B Concentrations and Renal Toxicity in Male Rats

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01223-08 ◽

2008 ◽

Vol 52 (11) ◽

pp. 4212-4212

Author(s):

Verica Risovic ◽

Michael Boyd ◽

Eugene Choo ◽

Kishor M. Wasan

Keyword(s):

Amphotericin B ◽

Renal Toxicity ◽

Male Rats ◽

Oral Formulations

Download Full-text

Effects of Lipid-Based Oral Formulations on Plasma and Tissue Amphotericin B Concentrations and Renal Toxicity in Male Rats

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.47.10.3339-3342.2003 ◽

2003 ◽

Vol 47 (10) ◽

pp. 3339-3342 ◽

Cited By ~ 32

Author(s):

Verica Risovic ◽

Michael Boyd ◽

Eugene Choo ◽

Kishor M. Wasan

Keyword(s):

Amphotericin B ◽

Sodium Phosphate ◽

Oral Absorption ◽

Renal Toxicity ◽

Sodium Deoxycholate ◽

Male Rats ◽

Time Curve ◽

Lipid Complex ◽

Concentration Time ◽

Oral Formulations

ABSTRACT The purpose of this study was to determine the effects of various lipid and mixed-micelle formulations on the oral absorption and renal toxicity of amphotericin B (AMB) in rats. The maximum concentration of AMB in plasma and the area under the concentration-time curve for 0 to 24 h for AMB were elevated in rats administered triglyceride (TG)-rich AMB formulations in comparison to those in rats given (i) AMB preformulated as a micelle containing sodium deoxycholate with sodium phosphate as a buffer (DOC-AMB), (ii) an AMB-lipid complex suspension, or (iii) AMB solubilized in methanol. Furthermore, our findings suggest that AMB incorporated into TG-based oral formulations has less renal toxicity than DOC-AMB.

Download Full-text

Protective Role of Chichorium intybus Extract Against Renal Toxicity Induced by Magnetite Silver Nanoparticles in Male Rats

OnLine Journal of Biological Sciences ◽

10.3844/ojbsci.2021.251.260 ◽

2021 ◽

Vol 21 (3) ◽

pp. 251-260

Author(s):

Doha M. Beltagy ◽

Ehab Tousson ◽

Nabiha I Abdo ◽

Batoul M. Izzularab

Keyword(s):

Silver Nanoparticles ◽

Renal Toxicity ◽

Protective Role ◽

Male Rats

Download Full-text

Alleviation of doxorubicin-induced nephrotoxicity by Clerodendrum volubile leaf extract in Wistar rats: A preliminary study

Journal of Herbmed Pharmacology ◽

10.34172/jhp.2020.18 ◽

2020 ◽

Vol 9 (2) ◽

pp. 138-144 ◽

Cited By ~ 1

Author(s):

Olorunfemi Raphael Molehin

Keyword(s):

Leaf Extract ◽

Renal Damage ◽

Renal Toxicity ◽

Methanolic Extract ◽

Male Rats ◽

Control Group ◽

Antioxidant Parameters ◽

Group A ◽

Preliminary Study ◽

Pre Treatment

Introduction: Doxorubicin (DOX), a well-known chemotherapeutic drug, has been reported to induce numerous toxic side effects including renal toxicity. This preliminary study was designed to investigate the ameliorative effects of methanolic leaf extract of Clerodendrum volubile (MECV) against DOX-induced nephrotoxicity in rats. Methods: Thirty male rats were divided into five groups; (a) Control group: rats were given 0.9% NaCl as vehicle, (b) DOX group: a single dose of DOX (25 mg/kg; i.p.) was administered and the rats were sacrificed 4 days after DOX injection, (c-e) Methanolic extract of C. volubile (MECV)-treated DOX groups: rats were given MECV (at the doses of 125, 250 and 500 mg/kg/d), respectively for 12 consecutive days, 8 days before and 4 days after the DOX administration. Results: DOX injection caused a significant increase (P<0.05) in serum creatinine and urea levels. The levels of renal antioxidant parameters: glutathione peroxidase, superoxide dismutase (SOD), catalase (CAT) and reduced glutathione were significantly (P<0.05) decreased in DOX-intoxicated rats with concomitant elevation of malondialdehyde level. Pretreatment with MECV restored antioxidant status, attenuated oxidative stress and improved kidney function markers. Pre-treatment with MECVprotected renal tissues against DOX-induced nephrotoxicity. Conclusion: The ameliorative effects of C. volubile leaves on these renal biochemical parameters may be via its antioxidant action and may serve as a novel combination agent with DOX to limit its renal damage.

Download Full-text

Evaluation of the Nephroprotective Effect of Sambucus Nigra Total Extract in a Rat Experimental Model of Gentamicine Nephrotoxicity

Revista de Chimie ◽

10.37358/rc.19.6.7256 ◽

2019 ◽

Vol 70 (6) ◽

pp. 1971-1974

Author(s):

Rodica Ungur ◽

Roxana Buzatu ◽

Radu Lacatus ◽

Robert Cristian Purdoiu ◽

Georgiana Petrut ◽

...

Keyword(s):

Phenolic Compounds ◽

Experimental Model ◽

Renal Toxicity ◽

Antioxidant Effect ◽

Protective Effects ◽

Sambucus Nigra ◽

Total Extract ◽

Nephroprotective Effect

Elderflower contains flavonoles, the most important of them being quercetin. These molecules have an important antioxidant effect. The goal of our study was to evaluate which conservation technique of elderflower results the highest concentration of rutin and and phenolic compounds. Further we evaluated the protective effects of these extracts against nephrotoxicity induced by gentamicin in rats. Sambucus nigra extract demonstrates nephroprotective effects in gentamicin-induced renal toxicity, significantly improving outcome and evolution in rats.

Download Full-text

Ameliorating activity of ginger (Zingiber officinale) extract against lead induced renal toxicity in male rats

Journal of Food Science and Technology ◽

10.1007/s13197-011-0568-9 ◽

2011 ◽

Vol 51 (5) ◽

pp. 908-914 ◽

Cited By ~ 18

Author(s):

Y. Amarnath Reddy ◽

M. Chalamaiah ◽

B. Ramesh ◽

G. Balaji ◽

P. Indira

Keyword(s):

Renal Toxicity ◽

Zingiber Officinale ◽

Male Rats

Download Full-text

Protective effect of curcumin on busulfan-induced renal toxicity in male rats

Renal Failure ◽

10.1080/0886022x.2020.1818580 ◽

2020 ◽

Vol 42 (1) ◽

pp. 969-977

Author(s):

Mohammad-Amin Abdollahifar ◽

Vahid Ebrahimi ◽

Abbas Aliaghaei ◽

Amir Raoofi ◽

Amirreza Khosravi ◽

...

Keyword(s):

Protective Effect ◽

Renal Toxicity ◽

Male Rats

Download Full-text

Influence of Sevoflurane on the Metabolism and Renal Effects of Compound A in Rats

Anesthesiology ◽

10.1097/00000542-200512000-00012 ◽

2005 ◽

Vol 103 (6) ◽

pp. 1183-1188 ◽

Cited By ~ 18

Author(s):

Evan D. Kharasch ◽

Jesara L. Schroeder ◽

Pam Sheffels ◽

H Denny Liggitt

Keyword(s):

Serum Creatinine ◽

Renal Toxicity ◽

Urine Volume ◽

Male Rats ◽

Sevoflurane Anesthesia ◽

Urea Nitrogen ◽

Compound A ◽

Renal Effects ◽

Protein Excretion

Background The sevoflurane degradation product compound A is nephrotoxic in rats. In contrast, patient exposure to compound A during sevoflurane anesthesia has no clinically significant renal effects. The mechanism for this difference is incompletely understood. One possibility is that the metabolism and toxicity of compound A in humans is prevented by sevoflurane. However, the effect of sevoflurane on compound A metabolism and nephrotoxicity is unknown. Thus, the purpose of this investigation was to determine the effect of sevoflurane on the metabolism and renal toxicity of compound A in rats. Methods Male rats received 0.25 mmol/kg intraperitoneal compound A, alone and during sevoflurane anesthesia (3%, 1.3 minimum alveolar concentration, for 3 h). Compound A metabolites in urine were quantified, and renal function was evaluated by serum creatinine and urea nitrogen, urine volume, osmolality, protein excretion, and renal tubular histology. Results Sevoflurane coadministration with compound A inhibited compound A defluorination while increasing relative metabolism through pathways of sulfoxidation and beta-lyase-catalyzed metabolism, which mediate toxicity. Sevoflurane coadministration with compound A increased some (serum creatinine and urea nitrogen, and necrosis) but not other (urine volume, osmolality, and protein excretion) indices of renal toxicity. Conclusions Sevoflurane does not suppress compound A nephrotoxicity in rats in vivo. These results do not suggest that lack of nephrotoxicity in surgical patients exposed to compound A during sevoflurane anesthesia results from an inhibitory effect of sevoflurane on compound A metabolism and toxicity. Rather, these results are consistent with differences between rats and humans in compound A exposure and inherent susceptibility to compound A nephrotoxicity.

Download Full-text