Enhanced enzymatic saccharification of rice straw by microwave pretreatment

2009 ◽  
Vol 100 (3) ◽  
pp. 1279-1284 ◽  
Author(s):  
Huan Ma ◽  
Wei-Wei Liu ◽  
Xing Chen ◽  
Yue-Jin Wu ◽  
Zeng-Liang Yu
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Microwave Pretreatment

scholarly journals A semi-dominant mutation in OsCESA9 improves biomass enzymatic digestibility and salt tolerance by relatively remodeling cell walls in rice

2020 ◽  
Author(s):  
Yafeng Ye ◽  
Shuoxun Wang ◽  
Kun Wu ◽  
Yan Ren ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Cellulose Content ◽  
Normal Plant ◽  
Wild Type ◽  
Negative Effects ◽  
Straw Return ◽  
Almost All

Abstract Background: Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another. Result: Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type (WT). Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging. Conclusion: Hence, manipulation of OsCESA9D387N can provide the perspective of the rice straw for biofuels and bioproducts due to its improved enzymatic saccharification.

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

scholarly journals Microwave pretreatment of lignocellulosic material in cholinium ionic liquid for efficient enzymatic saccharification

2014 ◽  
Vol 90 ◽  
pp. 90-95 ◽  
Author(s):  
Kazuaki Ninomiya ◽  
Takashi Yamauchi ◽  
Chiaki Ogino ◽  
Nobuaki Shimizu ◽  
Kenji Takahashi
Keyword(s):  
Ionic Liquid ◽  
Enzymatic Saccharification ◽  
Lignocellulosic Material ◽  
Microwave Pretreatment

Evaluation of Fungal Pretreatments for Enzymatic Saccharification of Rice Straw

2010 ◽  
Vol 43 (4) ◽  
pp. 401-405 ◽  
Author(s):  
Masayuki Taniguchi ◽  
Daisuke Takahashi ◽  
Daisuke Watanabe ◽  
Kenji Sakai ◽  
Kazuhiro Hoshino ◽  
...  
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification

Sulfite–formaldehyde pretreatment on rice straw for the improvement of enzymatic saccharification

2013 ◽  
Vol 142 ◽  
pp. 218-224 ◽  
Author(s):  
Feng Gu ◽  
Wangxia Wang ◽  
Lei Jing ◽  
Yongcan Jin
Keyword(s):  
Rice Straw ◽  
Enzymatic Saccharification

scholarly journals A semi-dominant mutation in OsCESA9 improves rice straw return to the field and increases salt tolerance by remodeling the cell wall in rice

2020 ◽  
Author(s):  
Yafeng Ye ◽  
Shuoxun Wang ◽  
Kun Wu ◽  
Yan Ren ◽  
Hongrui Jiang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Cellulose Content ◽  
Normal Plant ◽  
Wild Type ◽  
Negative Effects ◽  
Biomass Saccharification ◽  
Straw Return

Abstract Background Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another.Result Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type. Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging.Conclusion Hence, manipulation of OsCESA9D387N can enhance biomass saccharification and simultaneously facilitate the decay of rice straw in the soil, providing a new strategy for bioenergy crop breeding.

scholarly journals Production of Xyloligosaccharides from Rice Straw by Microwave-assisted Enzymatic Hydrolysis and Evaluation of Their Prebiotic Properties

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Alisa Pattarapisitporn ◽  
Nonglak Thiangthong ◽  
Pakorn Inthajak ◽  
Pannapapol Jaichakan ◽  
Wantana Panpa ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sugar Content ◽  
Lactobacillus Brevis ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Microwave Pretreatment ◽  
Pretreatment Time ◽  
Layer Chromatography

Rice straw (RS) is a by-product from rice production process. It is rich in cellulose, hemicellulose and lignin. RS hemicellulose mainly composes of arabinoxylan (AX). This research aimed to investigate the potential of microwave-pretreatment in AX extraction from RS and substrate to produce xylooligosaccharides (XOS) via enzymatic hydrolysis. The extractive-free RS was pretreated by microwave process at 160°C for 5-15 min, then the AX was extracted with 4% sodium hydroxide. The total sugar and reducing sugar content of AX exhibited that increasing microwave-pretreatment time increased the yield of AX. The highest AX content was found at 160°C for 10 min as 7.73%, reducing sugar content of 11.89 mg/g, and total sugar of 165.85 mg/g. The crude AX obtained by microwave-pretreatment was then used as a substrate for XOS production by two commercial xylanases of Pentopan mono BG (BG) and Ultraflo Max (UM), at the enzyme concentration of 50-300 U/g AX (50°C, pH 6.0) for 24 h.The reducing sugar content and sugar profiles were monitored by DNS assay,and thin layer chromatography (TLC) which revealed that BG 50 U/g at 12 h andUM 50 U/g at 24 h showed the promising reducing sugar of 16.4 and 25.44 mg/g,respectively. The composition of XOS derived from RS (RS-XOS) prepared by BGwas xylobiose (X2), xylotriose (X3), xylotretraose (X4), and xylopentaose (X5)while by UM was xylobiose (X2), xylotriose (X3) and xylotretraose (X5). Moreover,XOS produced by BG contained very low amount of xylose (X1). In addition, theRS-XOS could the growth of Lactobacillus brevis greater than commercial XOS.

Sign in / Sign up

Export Citation Format

Share Document